;AMINO TERMINAL 67KDA DOMAIN OF ESCHERICHIA COLI DNA TOPOISOMERASE I (RESIDUES 2-590 OF MATURE PROTEIN) CLONING ARTIFACT ADDS TWO RESIDUES TO THE AMINO-TERMINUS WHICH WERE NOT OBSERVED IN THE EXPERIMENTAL ELECTRON DENSITY (GLY-2, SER-1). ;

Method: X-RAY DIFFRACTION D_max: 101.3 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1ecl
deposition_date	1995-05-05
title	;AMINO TERMINAL 67KDA DOMAIN OF ESCHERICHIA COLI DNA TOPOISOMERASE I (RESIDUES 2-590 OF MATURE PROTEIN) CLONING ARTIFACT ADDS TWO RESIDUES TO THE AMINO-TERMINUS WHICH WERE NOT OBSERVED IN THE EXPERIMENTAL ELECTRON DENSITY (GLY-2, SER-1). ;
keywords	BACTERIAL TYPE I, DNA CLEAVAGE, STRAND PASSAGE, TOPOISOMERASE; TOPOISOMERASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	30.22 Å
rg_electron	29.80 Å
i0	64922900.00
molecular_weight	62516.0 kDa
excluded_volume	78055 Å³
envelope_volume	103520 Å³
shell_volume	30485 Å³
envelope_diameter	105.5 Å
shell_rg	35.17 Å
envelope_rg	29.68 Å
shape_rg	29.82 Å
total_rg	30.27 Å
total_atoms	4403
n_residues	552
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	101.3 Å
rg_real	30.32 Å
rg_real_error	0.80 Å
i0_real	6.4920e+07
i0_real_error	9.7610e+05
rg_reciprocal	30.28 Å
i0_reciprocal	64920000.0000
total_estimate	0.8813
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	33.1 Å
skewness	0.400
kurtosis	-0.374
angular_range	— – 0.2600 Å⁻¹
current_alpha	0.0000
highest_alpha	9482000.0000
n_real_points	53
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.888; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.904; Smooth: 0.886

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

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6. Citations (2)

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length_a	63.190 Å
length_b	77.970 Å
length_c	138.900 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	1.900 Å
resolution_low	5.000 Å
r_factor	0.2180
r_work	0.2180
r_free	—

description	ESCHERICHIA COLI TOPOISOMERASE I
formula_weight	67326.3 kDa
ec_number	5.99.1.2
mutation	GLY SER INSERTION AT N-TERMINUS, INSERTION OF STOP CODON AT 598; ENGINEERED
src_method	man
entity_count	1

type	polypeptide(L)
strand_id	A
atom_count	4939

;AMINO TERMINAL 67KDA DOMAIN OF ESCHERICHIA COLI DNA TOPOISOMERASE I (RESIDUES 2-590 OF MATURE PROTEIN) CLONING ARTIFACT ADDS TWO RESIDUES TO THE AMINO-TERMINUS WHICH WERE NOT OBSERVED IN THE EXPERIMENTAL ELECTRON DENSITY (GLY-2, SER-1). ;

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 water

6. Citations (2)

7. Files & Curves (10)

Files (7)

Curves (3)

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	536

fit	/app/data/saxs_computation/ec/1ecl/1ecl.fit
pddf	/app/data/pr_computation/ec/1ecl/1ecl.pr.out
profile	/app/data/saxs_computation/ec/1ecl/1ecl.dat

organism	Escherichia coli
ncbi_taxonomy_id	562