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1ee9

;CRYSTAL STRUCTURE OF THE NAD-DEPENDENT 5,10-METHYLENETETRAHYDROFOLATE DEHYDROGENASE FROM SACCHAROMYCES CEREVISIAE COMPLEXED WITH NAD ;

Method: X-RAY DIFFRACTION D_max: 65.0 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1ee9
deposition_date	2000-01-31
title	;CRYSTAL STRUCTURE OF THE NAD-DEPENDENT 5,10-METHYLENETETRAHYDROFOLATE DEHYDROGENASE FROM SACCHAROMYCES CEREVISIAE COMPLEXED WITH NAD ;
keywords	NUCLEOTIDE-BINDING DOMAIN, PROTEIN-NAD COMPLEX, MONOFUNCTIONAL, DEHYDROGENASE, FOLATE, OXIDOREDUCTASE; OXIDOREDUCTASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	20.81 Å
rg_electron	19.70 Å
i0	22658300.00
molecular_weight	36361.0 kDa
excluded_volume	45593 Å³
envelope_volume	52437 Å³
shell_volume	21990 Å³
envelope_diameter	66.3 Å
shell_rg	26.31 Å
envelope_rg	19.89 Å
shape_rg	19.71 Å
total_rg	20.55 Å
total_atoms	2555
n_residues	317
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	65.0 Å
rg_real	20.69 Å
rg_real_error	0.31 Å
i0_real	2.2660e+07
i0_real_error	2.7250e+05
rg_reciprocal	20.72 Å
i0_reciprocal	22660000.0000
total_estimate	0.9003
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	25.4 Å
skewness	0.172
kurtosis	-0.434
angular_range	— – 0.3800 Å⁻¹
current_alpha	0.0000
highest_alpha	4033000.0000
n_real_points	70
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.910; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.991; Smooth: 0.980

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	5,10-METHYLENETETRAHYDROFOLATE DEHYDROGENASE
formula_weight	36284.6 kDa
ec_number	1.5.1.15
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	2561

MSKPGRTILASKVAETFNTEIINNVEEYKKTHNGQGPLLVGFLANNDPAAKMYATWTQKTSESMGFRYDLRVIEDKDFLEEAIIQANGDDSVNGIMVYFPVFGNAQDQYLQQVVCKEKDVEGLNHVYYQNLYHNVRYLDKENRLKSILPCTPLAIVKILEFLKIYNNLLPEGNRLYGKKCIVINRSEIVGRPLAALLANDGATVYSVDVNNIQKFTRGESLKLNKHHVEDLGEYSEDLLKKCSLDSDVVITGVPSENYKFPTEYIKEGAVCINFACTKNFSDDVKEKASLYVPMTGKVTIAMLLRNMLRLVRNVELSKEK

Source

organism	Saccharomyces cerevisiae
common_name	;baker's yeast ;
ncbi_taxonomy_id	4932
host_organism	Escherichia coli

Entity 2 non-polymer

description	NICOTINAMIDE-ADENINE-DINUCLEOTIDE
formula_weight	663.4 kDa
src_method	syn
entity_count	1

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	6

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6. Citations (2)

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length_a	73.600 Å
length_b	73.600 Å
length_c	161.601 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	P 41 21 2
int_tables_number	92
crystal_system	—

resolution	3.000 Å
resolution_low	20.000 Å
r_factor	0.2540
r_work	0.2480
r_free	0.3600

fit	/app/data/saxs_computation/ee/1ee9/1ee9.fit
pddf	/app/data/pr_computation/ee/1ee9/1ee9.pr.out
profile	/app/data/saxs_computation/ee/1ee9/1ee9.dat

;CRYSTAL STRUCTURE OF THE NAD-DEPENDENT 5,10-METHYLENETETRAHYDROFOLATE DEHYDROGENASE FROM SACCHAROMYCES CEREVISIAE COMPLEXED WITH NAD ;

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction