← Back to search

1efn

HIV-1 NEF PROTEIN IN COMPLEX WITH R96I MUTANT FYN SH3 DOMAIN

Method: X-RAY DIFFRACTION D_max: 71.7 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	1efn
deposition_date	1996-06-29
title	HIV-1 NEF PROTEIN IN COMPLEX WITH R96I MUTANT FYN SH3 DOMAIN
keywords	;COMPLEX (SH3 DOMAIN-VIRAL ENHANCER), PROTO-ONCOGENE, TRANSFERASE, TYROSINE-PROTEIN KINASE, PHOSPHORYLATION, AIDS, MYRISTYLATION, GTP-BINDING, ATP-BINDING, SH3 DOMAIN, SH2 DOMAIN, PPII HELIX, PXXP MOTIF, COMPLEX (SH3 DOMAIN-VIRAL ENHANCER) complex ;; COMPLEX (SH3 DOMAIN/VIRAL ENHANCER)
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	23.34 Å
rg_electron	22.48 Å
i0	23300200.00
molecular_weight	37480.0 kDa
excluded_volume	46780 Å³
envelope_volume	56087 Å³
shell_volume	21244 Å³
envelope_diameter	73.1 Å
shell_rg	28.75 Å
envelope_rg	22.39 Å
shape_rg	22.42 Å
total_rg	23.42 Å
total_atoms	2643
n_residues	322
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	71.7 Å
rg_real	23.25 Å
rg_real_error	0.42 Å
i0_real	2.3300e+07
i0_real_error	3.1970e+05
rg_reciprocal	23.27 Å
i0_reciprocal	23300000.0000
total_estimate	0.9140
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	33.1 Å
skewness	0.128
kurtosis	-0.603
angular_range	— – 0.3400 Å⁻¹
current_alpha	0.0000
highest_alpha	3866000.0000
n_real_points	66
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.962; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.994

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (4)

Entity 1 polymer

description	FYN TYROSINE KINASE
formula_weight	6652.2 kDa
ec_number	2.7.1.112
mutation	R96I
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,C
atom_count	2742

ALFVALYDYEAITEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDS

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli BL21(DE3)

Entity 2 polymer

description	HIV-1 NEF PROTEIN
formula_weight	17809.0 kDa
mutation	T71R
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	B,D

ACAWLEAQEEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGENTSLLHPVSLHGMDDPEREVLEWRFDSRLAFHHVARELHPEYFKN

Source

organism	Human immunodeficiency virus 1
ncbi_taxonomy_id	11676
host_organism	Escherichia coli

Entity 3 non-polymer

description	TRIMETHYL LEAD ION
formula_weight	252.3 kDa
src_method	syn
entity_count	2

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	99

▶

6. Citations (3)

▶

length_a	107.800 Å
length_b	107.800 Å
length_c	229.100 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	24

space_group	P 65 2 2
int_tables_number	179
crystal_system	—

resolution	2.500 Å
resolution_low	6.000 Å
r_factor	0.2150
r_work	0.2150
r_free	0.2820

fit	/app/data/saxs_computation/ef/1efn/1efn.fit
pddf	/app/data/pr_computation/ef/1efn/1efn.pr.out
profile	/app/data/saxs_computation/ef/1efn/1efn.dat

HIV-1 NEF PROTEIN IN COMPLEX WITH R96I MUTANT FYN SH3 DOMAIN

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

Entity 4 water

6. Citations (3)

7. Files & Curves (10)

Files (7)

Curves (3)