Crystal structure of chi and psi subunit heterodimer from DNA POL III

entry_id	1em8
deposition_date	2000-03-16
title	Crystal structure of chi and psi subunit heterodimer from DNA POL III
keywords	DNA Pol III, heterodimer, clamp-loader, alpha-beta fold, GENE REGULATION; GENE REGULATION
method	X-RAY DIFFRACTION

rg_guinier	33.43 Å
rg_electron	33.15 Å
i0	50185000.00
molecular_weight	56254.0 kDa
excluded_volume	70421 ų
envelope_volume	96577 ų
shell_volume	25174 ų
envelope_diameter	126.9 Å
shell_rg	38.28 Å
envelope_rg	32.40 Å
shape_rg	33.13 Å
total_rg	33.67 Å
total_atoms	3969
n_residues	516
n_harmonics	20
q_range	— – 0.5000 Å−1
n_points	101
shell_type	directional
solvent_density	0.3340 e/ų
contrast_shell	0.0300 e/ų
crysol_version	4.1.3

dmax	117.3 Å
rg_real	33.60 Å
rg_real_error	1.64 Å
i0_real	5.0180e+07
i0_real_error	1.0910e+06
rg_reciprocal	33.49 Å
i0_reciprocal	50180000.0000
total_estimate	0.7047
solution_quality	REASONABLE a REASONABLE solution
n_peaks	4
r_peak_primary	22.5 Å
skewness	0.357
kurtosis	-0.463
angular_range	— – 0.2350 Å−1
current_alpha	0.0000
highest_alpha	19090000.0000
n_real_points	48
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.548; Stabil: 0.998; Sysdev: 1.000; Positv: 1.000; Valcen: 0.518; Smooth: 0.000

Unit Cell

length_a	64.377 Å
length_b	65.719 Å
length_c	73.429 Å
angle_alpha	90.00 °
angle_beta	116.15 °
angle_gamma	90.00 °
z_pdb	4

Symmetry

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

Refinement

resolution	2.100 Å
resolution_low	20.000 Å
r_factor	0.2330
r_work	0.2290
r_free	0.2650

Diffraction

temperature

100.0 K

Files (7)

• pr_distribution /app/data/pr_computation/em/1em8/pr_distribution.json
• pr_output /app/data/pr_computation/em/1em8/1em8.pr.out
• pr_plot /app/data/visualization/pr_computation/em/1em8/1em8_pr_distribution.png
• saxs_curve_dat /app/data/saxs_computation/em/1em8/1em8.dat
• saxs_fit /app/data/saxs_computation/em/1em8/1em8.fit
• saxs_log /app/data/saxs_computation/em/1em8/1em8.log
• saxs_plot /app/data/visualization/saxs_computation/em/1em8/1em8_saxs_profile.png

Curves (3)

fit	/app/data/saxs_computation/em/1em8/1em8.fit
pddf	/app/data/pr_computation/em/1em8/1em8.pr.out
profile	/app/data/saxs_computation/em/1em8/1em8.dat