SAXSdb — 1eof

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1. Protein Identity and Related Structures Protein Identity & Related Structures

POTASSIUM CHANNEL KV1.1

Aplysia californica

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count	Chain A； UniProt 66–165	Fragment：SHAKER T1 DOMAIN Mutation：N136A	No other associated polymer	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7.4；277 K；30% Isopropanol, .1 M Hepes 7.5, .2 M MgCl2, pH 7.4, VAPOR DIFFUSION, HANGING DROP	Resolution 2.38 Å R-free 0.264

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 1EOF	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
1A68 CRYSTAL STRUCTURE OF THE TETRAMERIZATION DOMAIN OF THE SHAKER POTASSIUM CHANNEL Deposited 1998-03-06	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 66–159（94 aa） Fragment：TETRAMERIZATION DOMAIN	Mutation：INSERTED MET AT N-TERMINUS	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 8.5；PROTEIN WAS CRYSTALLIZED FROM 7-8% PEG8000, 300 MM NACL, 200MM MGCL2, 20 MM TRIS, PH 8.5	Resolution 1.80 Å R-free 0.241
1EOD CRYSTAL STRUCTURE OF THE N136D MUTANT OF A SHAKER T1 DOMAIN Deposited 2000-03-22	Different mutation/modification Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 66–165（100 aa） Fragment：SHAKER T1 DOMAIN	Mutation：N136D	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；30% Isopropanol, .1 M Hepes 7.5. .2 M MgCl2,, VAPOR DIFFUSION, HANGING DROP, temperature 4K	Resolution 2.45 Å R-free 0.234
1EOE CRYSTAL STRUCTURE OF THE V135R MUTANT OF A SHAKER T1 DOMAIN Deposited 2000-03-22	Different mutation/modification Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 66–165（100 aa） Fragment：SHAKER T1 DOMAIN	Mutation：V135R	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；30% Isopropanol, .1 M Hepes 7.5, .2 M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 4K	Resolution 1.70 Å R-free 0.249
1T1D CRYSTAL STRUCTURE OF THE TETRAMERIZATION DOMAIN OF THE SHAKER POTASSIUM CHANNEL Deposited 1998-09-22	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 66–165（100 aa） Fragment：TETRAMERIZATION DOMAIN	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.5；24% ISOPROPANOL, .2M MGCL2, .1 M HEPES PH 7.5, 1MM DTT	Resolution 1.51 Å R-free 0.249

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	Q16968_APLCA
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–100； UniProt 66–165

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	1eof
Deposition date deposition_date	2000-03-22
Structure title title	CRYSTAL STRUCTURE OF THE N136A MUTANT OF A SHAKER T1 DOMAIN
Keywords keywords	POTASSIUM CHANNELS, APLYSIA KV1.1, PROTON TRANSPORT, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method method	X-RAY DIFFRACTION

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	14.76 Å
Radius of gyration Rg (electron density) rg_electron	13.33 Å
Forward intensity I(0) i0	2871980.00
Molecular weight molecular_weight	11863.0 kDa
Excluded volume excluded_volume	14839 Å³
Envelope volume envelope_volume	16423 Å³
Hydration-shell volume shell_volume	10762 Å³
Envelope diameter envelope_diameter	48.5 Å
Shell Rg shell_rg	18.71 Å
Envelope Rg envelope_rg	13.59 Å
Shape Rg shape_rg	13.29 Å
Total Rg total_rg	14.62 Å
Total atoms total_atoms	844
Residues n_residues	100
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	48.8 Å
Rg (real space) rg_real	14.69 Å
Rg uncertainty (real space) rg_real_error	0.34 Å
I(0) (real space) i0_real	2.8720e+06
I(0) uncertainty (real space) i0_real_error	2.9490e+04
Rg (reciprocal space) rg_reciprocal	14.70 Å
I(0) (reciprocal space) i0_reciprocal	2872000.0000
Solution quality estimate total_estimate	0.8750
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	2
Primary peak position r_peak_primary	18.5 Å
Skewness Skewness skewness	0.197
Kurtosis Kurtosis kurtosis	-0.255
Angular range angular_range	— – 0.5000 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	381300.0000
Real-space data points n_real_points	80
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.794; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.989

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (2)

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7. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

Domain ID domain_id	d1eofa_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.42 — POZ domain
Superfamily Superfamily superfamily	d.42.1 — POZ domain
Family Family family	d.42.1.2 — Tetramerization domain of potassium channels

CATH v4.4 (1 domains)

Domain ID domain_id	1eofA00
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	710 — Potassium Channel Kv1.1; Chain A
Homologous superfamily homologous superfamily	10 — Potassium Channel Kv1.1; Chain A

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8. Citations (1)

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Unit-cell length a length_a	59.384 Å
Unit-cell length b length_b	59.384 Å
Unit-cell length c length_c	146.894 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	16

Space group space_group	I 4 2 2
Space-group number int_tables_number	97
Crystal system crystal_system	—

Resolution resolution	2.380 Å
Low-resolution limit resolution_low	14.900 Å
R factor r_factor	0.2250
R_work r_work	0.2250
R_free r_free	0.2640

Description description	POTASSIUM CHANNEL KV1.1
Molecular weight formula_weight	12054.4 kDa
Mutation mutation	N136A
Source method src_method	man
Copy count entity_count	1

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	886

CRYSTAL STRUCTURE OF THE N136A MUTANT OF A SHAKER T1 DOMAIN

1. Protein Identity and Related Structures Protein Identity & Related Structures

POTASSIUM CHANNEL KV1.1

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

6. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

来源 Source

Entity 2 Entity 2 water

7. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

CATH v4.4 (1 domains)

8. Citations (1)

9. Files and Curves (10)

Files (7)

Curves (3)

来源生物 organism	Aplysia californica
通用名 common_name	California sea hare
NCBI Class编号 ncbi_taxonomy_id	6500
宿主生物 host_organism	Escherichia coli BL21(DE3)

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	42

fit	/app/data/saxs_computation/eo/1eof/1eof.fit
pddf	/app/data/pr_computation/eo/1eof/1eof.pr.out
profile	/app/data/saxs_computation/eo/1eof/1eof.dat