SAXSdb — 1fok

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1. Protein Identity and Related Structures Protein Identity & Related Structures

PROTEIN (FOKI RESTRICTION ENDONUCLEASE)

Planomicrobium okeanokoites

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–DNA	Monomer	Protein 1 DNA 2	;DNA (5'-D(TPCPGPGPAPTPGPAPTPAPAPCPGPCPTPAPG PTPCPA)-3') ; × 1 ;DNA (5'-D(APTPGPAPCPTPAPGPCPGPTPTPAPTPCPAPT PCPCPG)-3') ; × 1 water × 3	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	T2F1_FLAOK
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain A； PDBConstruct 1–576； UniProt 8–583

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	1fok
Deposition date deposition_date	1997-04-18
Structure title title	STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI BOUND TO DNA
Keywords keywords	;COMPLEX (ENDONUCLEASE-DNA), TYPE IIS, RESTRICTION ENDONUCLEASE, DEOXYRIBONUCLEASE, DNA HYDROLYSIS, DNA CLEAVAGE, HYDROLASE-DNA COMPLEX ;; HYDROLASE/DNA
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1fok__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1fok__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

1fok__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	27.76 Å
Rg (electron density)	26.87 Å
Total Rg	27.65 Å
Atom count	5355
Residues	608
Excluded volume	93375 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`1fok__assembly_1__model_1`	trimeric （3）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Description description	;DNA (5'-D(TPCPGPGPAPTPGPAPTPAPAPCPGPCPTPAPG PTPCPA)-3') ;
Molecular weight formula_weight	6158.0 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	B
Atom count atom_count	5527

TCGGATGATAACGCTAGTCA

Source Source

Source organism organism	Planomicrobium okeanokoites
NCBI taxonomy ID ncbi_taxonomy_id	244
Host organism host_organism	Escherichia coli

Entity 2 Entity 2 polymer

Description description	;DNA (5'-D(APTPGPAPCPTPAPGPCPGPTPTPAPTPCPAPT PCPCPG)-3') ;
Molecular weight formula_weight	6109.0 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	C

ATGACTAGCGTTATCATCCG

Entity 3 Entity 3 polymer

Description description	PROTEIN (FOKI RESTRICTION ENDONUCLEASE)
Molecular weight formula_weight	65519.2 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A

KIRTFGWVQNPGKFENLKRVVQVFDRNSKVHNEVKNIKIPTLVKESKIQKELVAIMNQHDLIYTYKELVGTGTSIRSEAPCDAIIQATIADQGNKKGYIDNWSSDGFLRWAHALGFIEYINKSDSFVITDVGLAYSKSADGSAIEKEILIEAISSYPPAIRILTLLEDGQHLTKFDLGKNLGFSGESGFTSLPEGILLDTLANAMPKDKGEIRNNWEGSSDKYARMIGGWLDKLGLVKQGKKEFIIPTLGKPDNKEFISHAFKITGEGLKVLRRAKGSTKFTRVPKRVYWEMLATNLTDKEYVRTRRALILEILIKAGSLKIEQIQDNLKKLGFDEVIETIENDIKGLINTGIFIEIKGRFYQLKDHILQFVIPNRGVTKQLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF

Source Source

Source organism organism	Planomicrobium okeanokoites
NCBI taxonomy ID ncbi_taxonomy_id	244

Entity 4 Entity 4 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	172

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6. Fold Classification (SCOP + CATH) 7 domains

SCOP 2.08 (4 domains)

Domain ID domain_id	d1foka1
Class class	a — All alpha proteins
Fold Fold fold	a.4 — DNA/RNA-binding 3-helical bundle
Superfamily Superfamily superfamily	a.4.5 — 'Winged helix' DNA-binding domain
Family Family family	a.4.5.12 — Restriction endonuclease FokI, N-terminal (recognition) domain

Domain ID domain_id	d1foka2
Class class	a — All alpha proteins
Fold Fold fold	a.4 — DNA/RNA-binding 3-helical bundle
Superfamily Superfamily superfamily	a.4.5 — 'Winged helix' DNA-binding domain
Family Family family	a.4.5.12 — Restriction endonuclease FokI, N-terminal (recognition) domain

Domain ID domain_id	d1foka3
Class class	a — All alpha proteins
Fold Fold fold	a.4 — DNA/RNA-binding 3-helical bundle
Superfamily Superfamily superfamily	a.4.5 — 'Winged helix' DNA-binding domain
Family Family family	a.4.5.12 — Restriction endonuclease FokI, N-terminal (recognition) domain

Domain ID domain_id	d1foka4
Class class	c — Alpha and beta proteins (a/b)
Fold Fold fold	c.52 — Restriction endonuclease-like
Superfamily Superfamily superfamily	c.52.1 — Restriction endonuclease-like
Family Family family	c.52.1.12 — Restriction endonuclease FokI, C-terminal (catalytic) domain

CATH v4.4 (3 domains)

Domain ID domain_id	1fokA01
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	241 — FokI Restriction Endonuclease; Chain A, domain 1
Homologous superfamily homologous superfamily	10 — Foki Restriction Endonuclease, Chain A, domain 1

Domain ID domain_id	1fokA02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	10 — Arc Repressor Mutant, subunit A
Homologous superfamily homologous superfamily	10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Domain ID domain_id	1fokA03
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	91 — Restriction Endonuclease
Homologous superfamily homologous superfamily	30 —

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Unit-cell length a length_a	65.590 Å
Unit-cell length b length_b	119.340 Å
Unit-cell length c length_c	71.520 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	101.42 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	2

Space group space_group	P 1 21 1
Space-group number int_tables_number	4
Crystal system crystal_system	—

Resolution resolution	2.800 Å
Low-resolution limit resolution_low	8.000 Å
R factor r_factor	0.2140
R_work r_work	0.2140
R_free r_free	0.2960

STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI BOUND TO DNA

1. Protein Identity and Related Structures Protein Identity & Related Structures

PROTEIN (FOKI RESTRICTION ENDONUCLEASE)

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1fok__assembly_1__model_1

1fok__assembly_1__model_1 | I(q)

1fok__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Source Source

Entity 4 Entity 4 water

6. Fold Classification (SCOP + CATH) 7 domains

SCOP 2.08 (4 domains)

CATH v4.4 (3 domains)

7. Citations (2)

STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI BOUND TO DNA

1. Protein Identity and Related Structures Protein Identity & Related Structures

PROTEIN (FOKI RESTRICTION ENDONUCLEASE)

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1fok__assembly_1__model_1

1fok__assembly_1__model_1 | I(q)

1fok__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Source Source

Entity 4 Entity 4 water

6. Fold Classification (SCOP + CATH) 7 domains

SCOP 2.08 (4 domains)

CATH v4.4 (3 domains)

7. Citations (2)