1fx0

Crystal structure of the chloroplast F1-ATPase from spinach

Method: X-RAY DIFFRACTION Dmax: 100.4 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 1fx0

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1fx0
下载 Download

1. 结构基本信息 1. Structure Basics

条目编号 entry_id1fx0
沉积日期 deposition_date2000-09-25
结构标题 titleCrystal structure of the chloroplast F1-ATPase from spinach
关键词 keywordslatent ATPase, thermal stability, potential tentoxin binding site, HYDROLASE; HYDROLASE
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier31.11
回转半径 Rg (电子) rg_electron30.16
零角强度 I(0) i0162768000.00
分子量 molecular_weight102250.0 kDa
排除体积 excluded_volume128530 ų
包络体积 envelope_volume159000 ų
水化壳体积 shell_volume43132 ų
包络直径 envelope_diameter107.6
壳层 Rg shell_rg38.11
包络 Rg envelope_rg30.35
形状 Rg shape_rg30.14
总 Rg total_rg30.90
总原子数 total_atoms7187
残基数 n_residues944
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax100.4
Rg (实空间) rg_real31.03
Rg 误差 (实空间) rg_real_error0.67
I(0) (实空间) i0_real1.6280e+08
I(0) 误差 (实空间) i0_real_error2.4170e+06
Rg (倒空间) rg_reciprocal31.07
I(0) (倒空间) i0_reciprocal162800000.0000
解质量估计 total_estimate0.8959
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary37.4
偏度 Skewness skewness0.288
峰度 Kurtosis kurtosis-0.389
角度范围 angular_range— – 0.2550 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha54170000.0000
实空间数据点数 n_real_points52
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.896; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.956

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (2)

6. 折叠分类 (SCOP + CATH) 12 domains

SCOP 2.08 (6 domains)

结构域编号 domain_idd1fx0a1
类 Class classa — All alpha proteins
折叠类型 Fold folda.69 — Left-handed superhelix
超家族 Superfamily superfamilya.69.1 — C-terminal domain of alpha and beta (or A/B) subunits of rotary ATPases
家族 Family familya.69.1.1 — C-terminal domain of alpha and beta subunits of F1 ATP synthase
结构域编号 domain_idd1fx0a2
类 Class classb — All beta proteins
折叠类型 Fold foldb.49 — Domain of alpha and beta subunits of F1 ATP synthase-like
超家族 Superfamily superfamilyb.49.1 — N-terminal domain of alpha and beta (or A/B) subunits of rotary ATPases
家族 Family familyb.49.1.1 — N-terminal domain of alpha and beta subunits of F1 ATP synthase
结构域编号 domain_idd1fx0a3
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
超家族 Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
家族 Family familyc.37.1.11 — RecA protein-like (ATPase-domain)
结构域编号 domain_idd1fx0b1
类 Class classa — All alpha proteins
折叠类型 Fold folda.69 — Left-handed superhelix
超家族 Superfamily superfamilya.69.1 — C-terminal domain of alpha and beta (or A/B) subunits of rotary ATPases
家族 Family familya.69.1.1 — C-terminal domain of alpha and beta subunits of F1 ATP synthase
结构域编号 domain_idd1fx0b2
类 Class classb — All beta proteins
折叠类型 Fold foldb.49 — Domain of alpha and beta subunits of F1 ATP synthase-like
超家族 Superfamily superfamilyb.49.1 — N-terminal domain of alpha and beta (or A/B) subunits of rotary ATPases
家族 Family familyb.49.1.1 — N-terminal domain of alpha and beta subunits of F1 ATP synthase
结构域编号 domain_idd1fx0b3
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
超家族 Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
家族 Family familyc.37.1.11 — RecA protein-like (ATPase-domain)

CATH v4.4 (6 domains)

结构域编号 domain_id1fx0A01
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology30 — Elongation Factor Tu (Ef-tu); domain 3
同源超家族 H-superfamily homologous superfamily20
结构域编号 domain_id1fx0A02
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
结构域编号 domain_id1fx0A03
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology150 — Lysin
同源超家族 H-superfamily homologous superfamily20 — ATP synthase alpha/beta chain, C-terminal domain
结构域编号 domain_id1fx0B01
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology10 — Thrombin, subunit H
同源超家族 H-superfamily homologous superfamily170
结构域编号 domain_id1fx0B02
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
结构域编号 domain_id1fx0B03
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology1140 — Bovine Mitochondrial F1-ATPase, ATP Synthase Beta Chain; Chain D, domain3
同源超家族 H-superfamily homologous superfamily10 — Bovine Mitochondrial F1-atpase; Atp Synthase Beta Chain; Chain D, domain 3

7. 引用文献 (2)

8. 文件与曲线 (10)