1gxg

Non-cognate protein-protein interactions: the NMR structure of the colicin E8 inhibitor protein Im8 and its interaction with the DNase domain of colicin E9

Method: SOLUTION NMR

1. Protein Identity and Related Structures Protein Identity & Related Structures

COLICIN E8 IMMUNITY PROTEIN

ESCHERICHIA COLI

UniProt P09881

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein monomer Monomer Protein 1 No other associated polymer Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name IMM8_ECOLI
Isoform
PDB entities 1
Chains and sequence ranges Author chain A; PDBConstruct 1–85; UniProt 1–85

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

2. Structure Basics 2. Structure Basics

Entry ID entry_id1gxg
Deposition date deposition_date2002-04-04
Structure title titleNon-cognate protein-protein interactions: the NMR structure of the colicin E8 inhibitor protein Im8 and its interaction with the DNase domain of colicin E9
Keywords keywordsINHIBITOR, INHIBITOR PROTEIN OF DNASE COLICIN E8, BACTERIOCIN IMMUNITY, PLASMID; INHIBITOR
Experimental Method methodSOLUTION NMR

3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1gxg__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1gxg__assembly_1__model_1 | I(q)

10-2 10-1 105 106 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

1gxg__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg(Guinier)13.41 Å
Rg (electron density)11.89 Å
Total Rg13.19 Å
Atom count1316
Residues85
Excluded volume11836 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 1gxg__assembly_1__model_1 monomeric (1) Success 4.1.3-1-20251215 (887e7ef) View Download

4. Crystallography and Experiment 4. Crystallography & Experiment

5. Entities and Polymers Entities & Polymers (1)

6. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

Domain ID domain_idd1gxga_
Class classa — All alpha proteins
Fold Fold folda.28 — Acyl carrier protein-like
Superfamily Superfamily superfamilya.28.2 — Colicin E immunity proteins
Family Family familya.28.2.1 — Colicin E immunity proteins

CATH v4.4 (1 domains)

Domain ID domain_id1gxgA00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology1200 — Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein; Chain A
Homologous superfamily homologous superfamily20 — Colicin E immunity protein

7. Citations (1)