SAXSdb — 1hk9

▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

PROTEIN HFQ

ESCHERICHIA COLI

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein homooligomer Homooligomer Protein × 6 PDB declaration: hexameric（6） Consistent with protein copy count	Chain A； UniProt 1–72 Chain B； UniProt 1–72 Chain C； UniProt 1–72 Chain D； UniProt 1–72 Chain E； UniProt 1–72 Chain F； UniProt 1–72	Fragment：RESIDUES 1-72	No other associated polymer	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 4.6；293 K；CRYSTALS WERE OBTAINED BY VAPOR DIFFUSION IN 2UL SITTING DROPS. THE RESERVOIR CONTAINED 25% PEG 4000, 0.2 M NH4-ACETATE AND 0.2 M NA-ACETATE PH 4.6. CRYSTALLIZATION WERE CARRIED OUT AT 20C.	Resolution 2.15 Å R-free 0.262

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 1HK9	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
2Y90 Crystal structure of Hfq riboregulator from E. coli (P6 space group) Deposited 2011-02-11	Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 1–102（102 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；CRYSTALS WERE OBTAINED BY VAPOR DIFFUSION IN 2UL HANGING DROPS AT 20C. THE RESERVOIR CONTAINED 1.6 M AMMONIUM SULFATE, 0.1 M TRIS-HCL PH 8.0.	Resolution 2.25 Å R-free 0.226
2YHT Crystal structure of Hfq riboregulator from E. coli (P1 space group) Deposited 2011-05-06	Different construct Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 1–72（72 aa） Fragment：SM-LIKE FOLD, RESIDUES 1-72 Chain B 1–72（72 aa） Fragment：SM-LIKE FOLD, RESIDUES 1-72 Chain C 1–72（72 aa） Fragment：SM-LIKE FOLD, RESIDUES 1-72 Chain D 1–72（72 aa） Fragment：SM-LIKE FOLD, RESIDUES 1-72 Chain E 1–72（72 aa） Fragment：SM-LIKE FOLD, RESIDUES 1-72 Chain F 1–72（72 aa） Fragment：SM-LIKE FOLD, RESIDUES 1-72	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.4；293 K；CRYSTALS WERE OBTAINED BY VAPOR DIFFUSION IN 2UL SITTING DROPS. THE RESERVOIR CONTAINED 30% PEG 3350, 0.1 M NA-CITRATE PH 5.4. CRYSTALLIZATIONS WERE CARRIED OUT AT 20 DEGREES CELSIUS.	Resolution 2.90 Å R-free 0.229
2YHT Crystal structure of Hfq riboregulator from E. coli (P1 space group) Deposited 2011-05-06	Different construct Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain G 1–72（72 aa） Fragment：SM-LIKE FOLD, RESIDUES 1-72 Chain H 1–72（72 aa） Fragment：SM-LIKE FOLD, RESIDUES 1-72 Chain I 1–72（72 aa） Fragment：SM-LIKE FOLD, RESIDUES 1-72 Chain J 1–72（72 aa） Fragment：SM-LIKE FOLD, RESIDUES 1-72 Chain K 1–72（72 aa） Fragment：SM-LIKE FOLD, RESIDUES 1-72 Chain L 1–72（72 aa） Fragment：SM-LIKE FOLD, RESIDUES 1-72	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.4；293 K；CRYSTALS WERE OBTAINED BY VAPOR DIFFUSION IN 2UL SITTING DROPS. THE RESERVOIR CONTAINED 30% PEG 3350, 0.1 M NA-CITRATE PH 5.4. CRYSTALLIZATIONS WERE CARRIED OUT AT 20 DEGREES CELSIUS.	Resolution 2.90 Å R-free 0.229
3GIB Crystal Structure of the Complex of the E. coli Hfq with Poly(A) Deposited 2009-03-05	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Homooligomer；Protein × 6 PDB declaration: octameric	Chain A 2–69（68 aa） Fragment：N-terminal fragment (2-69) Chain B 2–69（68 aa） Fragment：N-terminal fragment (2-69) Chain C 2–69（68 aa） Fragment：N-terminal fragment (2-69)	Not recorded	NHE 2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID × 6	X-RAY DIFFRACTION X-ray crystallization conditions pH 9.5；298 K；40% MPD, 0.1 M CHES 9.5, hanging drop, temperature 298K	Resolution 2.40 Å R-free 0.259
3QHS Crystal structure of full-length Hfq from Escherichia coli Deposited 2011-01-26	Different construct Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 1–102（102 aa） Chain B 1–102（102 aa） Chain C 1–102（102 aa） Chain D 1–102（102 aa） Chain E 1–102（102 aa） Chain F 1–102（102 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；30% v/v PEG 550 MME, 0.05M calcium chloride dihydrate, 0.1M BIS-TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	Resolution 2.85 Å R-free 0.250
3QHS Crystal structure of full-length Hfq from Escherichia coli Deposited 2011-01-26	Different construct Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain G 1–102（102 aa） Chain H 1–102（102 aa） Chain I 1–102（102 aa） Chain J 1–102（102 aa） Chain K 1–102（102 aa） Chain L 1–102（102 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；30% v/v PEG 550 MME, 0.05M calcium chloride dihydrate, 0.1M BIS-TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	Resolution 2.85 Å R-free 0.250
3QO3 Crystal structure of Escherichia coli Hfq, in complex with ATP Deposited 2011-02-09	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 1–65（65 aa） Fragment：N-terminal (L)Sm core-domain, UNP residues 1-65 Chain B 1–65（65 aa） Fragment：N-terminal (L)Sm core-domain, UNP residues 1-65 Chain C 1–65（65 aa） Fragment：N-terminal (L)Sm core-domain, UNP residues 1-65 Chain D 1–65（65 aa） Fragment：N-terminal (L)Sm core-domain, UNP residues 1-65 Chain E 1–65（65 aa） Fragment：N-terminal (L)Sm core-domain, UNP residues 1-65 Chain F 1–65（65 aa） Fragment：N-terminal (L)Sm core-domain, UNP residues 1-65	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M Hepes, 10% (W/V) PEG 8000, 8% (V/V) Ethylene glycol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K	Resolution 2.15 Å R-free 0.257
3VU3 Crystal structure of the Hfq and catalase HPII complex Deposited 2012-06-15	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric	Chain C 1–102（102 aa） Chain D 1–102（102 aa） Chain E 1–102（102 aa） Chain F 1–102（102 aa） Chain G 1–102（102 aa） Chain H 1–102（102 aa）	Not recorded	HEM PROTOPORPHYRIN IX CONTAINING FE × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；293 K；0.1M Tris-HCl, 0.18M NaCl, 10% PEG4000, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 2.85 Å R-free 0.246
4JRI Crystal Structure of Escherichia coli Hfq Proximal Edge Mutant Deposited 2013-03-21	Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 2–69（68 aa） Fragment：UNP residues 2-69 Chain B 2–69（68 aa） Fragment：UNP residues 2-69	Mutation：F39W Mutation：F39W	No recorded non-water small molecule	X-RAY DIFFRACTION mmCIF provides none of the parsed conditions	Resolution 1.83 Å R-free 0.257
4JRI Crystal Structure of Escherichia coli Hfq Proximal Edge Mutant Deposited 2013-03-21	Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain C 2–69（68 aa） Fragment：UNP residues 2-69 Chain D 2–69（68 aa） Fragment：UNP residues 2-69	Mutation：F39W Mutation：F39W	No recorded non-water small molecule	X-RAY DIFFRACTION mmCIF provides none of the parsed conditions	Resolution 1.83 Å R-free 0.257
4JRK Crystal Structure of Escherichia coli Hfq Surface Mutant Deposited 2013-03-21	Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 2–69（68 aa） Fragment：UNP residues 2-69 Chain B 2–69（68 aa） Fragment：UNP residues 2-69 Chain C 2–69（68 aa） Fragment：UNP residues 2-69	Mutation：F11W Mutation：F11W Mutation：F11W	No recorded non-water small molecule	X-RAY DIFFRACTION mmCIF provides none of the parsed conditions	Resolution 1.89 Å R-free 0.216
4JUV Crystal Structure of Escherichia coli Hfq Distal Face 1 Mutant Deposited 2013-03-25	Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 2–69（68 aa） Chain B 2–69（68 aa） Chain C 2–69（68 aa） Chain D 2–69（68 aa） Chain E 2–69（68 aa） Chain F 2–69（68 aa）	Mutation：Y25W Mutation：Y25W Mutation：Y25W Mutation：Y25W Mutation：Y25W Mutation：Y25W	No recorded non-water small molecule	X-RAY DIFFRACTION mmCIF provides none of the parsed conditions	Resolution 2.19 Å
4RCB Crystal structure of E Coli Hfq Deposited 2014-09-15	Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 5–71（67 aa） Fragment：UNP RESIDUES 5-71	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；0.5M Lithium chloride, 1.6M Ammonium sulfate , VAPOR DIFFUSION, SITTING DROP	Resolution 1.63 Å R-free 0.248
4RCC Crystal structure of E Coli Hfq Deposited 2014-09-15	Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 5–71（67 aa） Fragment：UNP RESIDUES 5-71	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；0.1M TRIS.HCl pH 8.5, 1.5M Ammonium sulfate, 15 %(w/v) Glycerol, VAPOR DIFFUSION, SITTING DROP	Resolution 1.98 Å R-free 0.213
4V2S Crystal structure of Hfq in complex with the sRNA RydC Deposited 2014-10-14	Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Homooligomer；Protein × 6 PDB declaration: heptameric	Chain A 1–102（102 aa） Chain B 1–102（102 aa） Chain C 1–102（102 aa） Chain D 1–102（102 aa） Chain E 1–102（102 aa） Chain F 1–102（102 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 6.5；0.2 M TRI-SODIUM CITRATE, 0.1 M SODIUM CACODYLATE (PH 6.5), 15% ISOPROPANOL	Resolution 3.48 Å R-free 0.282
9GU5 Crystal Structure of Hfq V22A Deposited 2024-09-18	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Homooligomer；Protein × 6 PDB declaration: heptameric	Chain A 1–102（102 aa） Chain B 1–102（102 aa） Chain C 1–102（102 aa） Chain D 1–102（102 aa） Chain E 1–102（102 aa） Chain F 1–102（102 aa）	Mutation：V22A Mutation：V22A Mutation：V22A Mutation：V22A Mutation：V22A Mutation：V22A	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；22.5 %v/v PEGSB, 0.2 M LiSO4, 0.05 M Zn Acet, 0.1 M BIS-TRIS	Resolution 2.90 Å R-free 0.387
9GU5 Crystal Structure of Hfq V22A Deposited 2024-09-18	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain G 1–102（102 aa） Chain H 1–102（102 aa） Chain I 1–102（102 aa） Chain J 1–102（102 aa） Chain K 1–102（102 aa） Chain L 1–102（102 aa）	Mutation：V22A Mutation：V22A Mutation：V22A Mutation：V22A Mutation：V22A Mutation：V22A	MG MAGNESIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；22.5 %v/v PEGSB, 0.2 M LiSO4, 0.05 M Zn Acet, 0.1 M BIS-TRIS	Resolution 2.90 Å R-free 0.387
9H45 Crystal Structure of Hfq V22A Deposited 2024-10-17	Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein–RNA Homooligomer；Protein × 6 PDB declaration: heptameric	Chain A 1–102（102 aa） Chain B 1–102（102 aa） Chain C 1–102（102 aa） Chain D 1–102（102 aa） Chain E 1–102（102 aa） Chain F 1–102（102 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；15 %v/v PEGSH 0.15 M NH4 Acet 0.1 M Na3 Cit	Resolution 2.08 Å R-free 0.323

14 other PDB entries and 18 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	HFQ_ECOLI
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 3–74； UniProt 1–72 Author chain B； PDBConstruct 3–74； UniProt 1–72 Author chain C； PDBConstruct 3–74； UniProt 1–72 Author chain D； PDBConstruct 3–74； UniProt 1–72 Author chain E； PDBConstruct 3–74； UniProt 1–72 Author chain F； PDBConstruct 3–74； UniProt 1–72

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id	1hk9
Deposition date deposition_date	2003-03-06
Structure title title	Crystal structure of the Hfq protein from Escherichia coli
Keywords keywords	RNA-BINDING PROTEIN, SM-LIKE, PLEIOTROPIC REGULATOR, RNA BINDING PROTEIN, RNA CHAPERONE; RNA BINDING PROTEIN
Experimental Method method	X-RAY DIFFRACTION

▼

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	23.95 Å
Radius of gyration Rg (electron density) rg_electron	22.48 Å
Forward intensity I(0) i0	29377100.00
Molecular weight molecular_weight	43976.0 kDa
Excluded volume excluded_volume	56150 Å³
Envelope volume envelope_volume	66988 Å³
Hydration-shell volume shell_volume	24544 Å³
Envelope diameter envelope_diameter	70.4 Å
Shell Rg shell_rg	29.42 Å
Envelope Rg envelope_rg	22.46 Å
Shape Rg shape_rg	22.45 Å
Total Rg total_rg	23.43 Å
Total atoms total_atoms	3104
Residues n_residues	394
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

▼

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	70.7 Å
Rg (real space) rg_real	23.84 Å
Rg uncertainty (real space) rg_real_error	0.35 Å
I(0) (real space) i0_real	2.9380e+07
I(0) uncertainty (real space) i0_real_error	3.6080e+05
Rg (reciprocal space) rg_reciprocal	23.86 Å
I(0) (reciprocal space) i0_reciprocal	29380000.0000
Solution quality estimate total_estimate	0.9165
Solution quality rating solution_quality	EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks	2
Primary peak position r_peak_primary	30.9 Å
Skewness Skewness skewness	0.153
Kurtosis Kurtosis kurtosis	-0.638
Angular range angular_range	— – 0.3300 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	14380000.0000
Real-space data points n_real_points	65
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.982; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.965

▶

5. Crystallography and Experiment 5. Crystallography & Experiment

▶

6. Entities and Polymers Entities & Polymers (2)

▼

7. Fold Classification (SCOP + CATH) 12 domains

SCOP 2.08 (6 domains)

Domain ID domain_id	d1hk9a_
Class class	b — All beta proteins
Fold Fold fold	b.38 — Sm-like fold
Superfamily Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
Family Family family	b.38.1.2 — Pleiotropic translational regulator Hfq

Domain ID domain_id	d1hk9b_
Class class	b — All beta proteins
Fold Fold fold	b.38 — Sm-like fold
Superfamily Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
Family Family family	b.38.1.2 — Pleiotropic translational regulator Hfq

Domain ID domain_id	d1hk9c_
Class class	b — All beta proteins
Fold Fold fold	b.38 — Sm-like fold
Superfamily Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
Family Family family	b.38.1.2 — Pleiotropic translational regulator Hfq

Domain ID domain_id	d1hk9d_
Class class	b — All beta proteins
Fold Fold fold	b.38 — Sm-like fold
Superfamily Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
Family Family family	b.38.1.2 — Pleiotropic translational regulator Hfq

Domain ID domain_id	d1hk9e_
Class class	b — All beta proteins
Fold Fold fold	b.38 — Sm-like fold
Superfamily Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
Family Family family	b.38.1.2 — Pleiotropic translational regulator Hfq

Domain ID domain_id	d1hk9f_
Class class	b — All beta proteins
Fold Fold fold	b.38 — Sm-like fold
Superfamily Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
Family Family family	b.38.1.2 — Pleiotropic translational regulator Hfq

CATH v4.4 (6 domains)

Domain ID domain_id	1hk9A00
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	1hk9B00
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	1hk9C00
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	1hk9D00
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	1hk9E00
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	1hk9F00
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	100 —

▶

8. Citations (6)

Sm-Like Proteins in Eubacteria: The Crystal Structure of the Hfq Protein from Escherichia Coli

Nucleic Acids Res. (2003)

PMID: 12853626 · DOI: 10.1093/NAR/GKG480 PRIMARY

The Sm-Like Hfq Protein Increases Oxys RNA Interaction with Target Mrnas

Mol.Cell (2002)

PMID: 11804582 · DOI: 10.1016/S1097-2765(01)00437-3

Hfq: A Bacterial Sm-Like Protein that Mediates RNA-RNA Interaction

Mol.Cell (2002)

PMID: 11804583 · DOI: 10.1016/S1097-2765(01)00436-1

Structures of the Pleiotropic Translational Regulator Hfq and an Hfq-RNA Complex: A Bacterial Sm-Like Protein

Embo J. (2002)

PMID: 12093755 · DOI: 10.1093/EMBOJ/CDF322

Characterization of Broadly Pleiotropic Phenotypes Caused by an Hfq Insertion Mutation in Escherichia Coli K-12

Mol.Microbiol. (1994)

PMID: 7984093 · DOI: 10.1111/J.1365-2958.1994.TB00400.X

;Bacterial Proteins Required for Replication of Phage Q Ribonucleic Acid. Purification and Properties of Host Factor I, a Ribonucleic Acid-Binding Protein ;

J.Biol.Chem. (1972)

PMID: 4550762

▶

Unit-cell length a length_a	61.350 Å
Unit-cell length b length_b	61.350 Å
Unit-cell length c length_c	166.100 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	120.00 °
Molecules per unit cell Z z_pdb	36

Space group space_group	P 61
Space-group number int_tables_number	169
Crystal system crystal_system	—

Resolution resolution	2.150 Å
Low-resolution limit resolution_low	20.000 Å
R factor r_factor	0.2080
R_work r_work	0.2080
R_free r_free	0.2620

Description description	PROTEIN HFQ
Molecular weight formula_weight	8257.6 kDa
Source method src_method	man
Copy count entity_count	6

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E,F
Atom count atom_count	3240

Crystal structure of the Hfq protein from Escherichia coli

1. Protein Identity and Related Structures Protein Identity & Related Structures

PROTEIN HFQ

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

6. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

来源 Source

Entity 2 Entity 2 water

7. Fold Classification (SCOP + CATH) 12 domains

SCOP 2.08 (6 domains)

CATH v4.4 (6 domains)

8. Citations (6)

9. Files and Curves (10)

Files (7)

Curves (3)

来源生物 organism	ESCHERICHIA COLI
NCBI Class编号 ncbi_taxonomy_id	562
宿主生物 host_organism	ESCHERICHIA COLI

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	136

fit	/app/data/saxs_computation/hk/1hk9/1hk9.fit
pddf	/app/data/pr_computation/hk/1hk9/1hk9.pr.out
profile	/app/data/saxs_computation/hk/1hk9/1hk9.dat