SAXSdb — 1iaw

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1. Protein Identity and Related Structures Protein Identity & Related Structures

TYPE II RESTRICTION ENZYME NAEI

Lechevalieria aerocolonigenes

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–DNA	Homooligomer	Protein 2 DNA 4	5'-D(TPGPCPCPAPCPGPCPCPGPGPCPGPTPGPGP*C)-3' × 4 water × 5	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	T2N1_NOCAE
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain A； PDBConstruct 1–317； UniProt 1–317 Author chain B； PDBConstruct 1–317； UniProt 1–317

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	1iaw
Deposition date deposition_date	2001-03-23
Structure title title	CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA
Keywords keywords	protein-DNA complex, HYDROLASE-DNA COMPLEX; HYDROLASE/DNA
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1iaw__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1iaw__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

1iaw__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	29.81 Å
Rg (electron density)	28.89 Å
Total Rg	29.57 Å
Atom count	6151
Residues	675
Excluded volume	103800 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`1iaw__assembly_1__model_1`	hexameric （6）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Description description	5'-D(TPGPCPCPAPCPGPCPCPGPGPCPGPTPGPGP*C)-3'
Molecular weight formula_weight	5205.3 kDa
Source method src_method	syn
Copy count entity_count	4

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	C,D,E,F
Atom count atom_count	6195

TGCCACGCCGGCGTGGC

Entity 2 Entity 2 polymer

Description description	TYPE II RESTRICTION ENZYME NAEI
Molecular weight formula_weight	35384.0 kDa
EC 编号 ec_number	3.1.21.4
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B

MTELPLQFAEPDDDLERVRATLYSLDPDGDRTAGVLRDTLDQLYDGQRTGRWNFDQLHKTEKTHMGTLVEINLHREFQFGDGFETDYEIAGVQVDCKFSMSQGAWMLPPESIGHICLVIWASDQQCAWTAGLVKVIPQFLGTANRDLKRRLTPEGRAQVVKLWPDHGKLQENLLLHIPGDVRDQIFSAKSSRGNQHGQARVNELFRRVHGRLIGRAVIATVAQQDDFMKRVRGSGGARSILRPEGIIILGHQDNDPKVANDLGLPVPRKGQVVAARVVPADEGDQRQTAEIQGRRWAVAVPGDPIVEAPVVPRKSAE

Source Source

Source organism organism	Lechevalieria aerocolonigenes
NCBI taxonomy ID ncbi_taxonomy_id	68170
Host organism host_organism	Escherichia coli

Entity 3 Entity 3 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	44

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6. Fold Classification (SCOP + CATH) 6 domains

SCOP 2.08 (2 domains)

Domain ID domain_id	d1iawa_
Class class	c — Alpha and beta proteins (a/b)
Fold Fold fold	c.52 — Restriction endonuclease-like
Superfamily Superfamily superfamily	c.52.1 — Restriction endonuclease-like
Family Family family	c.52.1.9 — Restriction endonuclease NaeI

Domain ID domain_id	d1iawb_
Class class	c — Alpha and beta proteins (a/b)
Fold Fold fold	c.52 — Restriction endonuclease-like
Superfamily Superfamily superfamily	c.52.1 — Restriction endonuclease-like
Family Family family	c.52.1.9 — Restriction endonuclease NaeI

CATH v4.4 (4 domains)

Domain ID domain_id	1iawA01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	600 — ECO RV Endonuclease; Chain A
Homologous superfamily homologous superfamily	10 — DNA mismatch repair MutH/Restriction endonuclease, type II

Domain ID domain_id	1iawA02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	10 — Arc Repressor Mutant, subunit A
Homologous superfamily homologous superfamily	10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Domain ID domain_id	1iawB01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	600 — ECO RV Endonuclease; Chain A
Homologous superfamily homologous superfamily	10 — DNA mismatch repair MutH/Restriction endonuclease, type II

Domain ID domain_id	1iawB02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	10 — Arc Repressor Mutant, subunit A
Homologous superfamily homologous superfamily	10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

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Unit-cell length a length_a	59.600 Å
Unit-cell length b length_b	65.000 Å
Unit-cell length c length_c	258.500 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	16

Space group space_group	P 21 21 21
Space-group number int_tables_number	19
Crystal system crystal_system	—

Resolution resolution	2.400 Å
Low-resolution limit resolution_low	99.000 Å
R factor r_factor	—
R_work r_work	0.2430
R_free r_free	0.2930

CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA

1. Protein Identity and Related Structures Protein Identity & Related Structures

TYPE II RESTRICTION ENZYME NAEI

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1iaw__assembly_1__model_1

1iaw__assembly_1__model_1 | I(q)

1iaw__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

Entity 3 Entity 3 water

6. Fold Classification (SCOP + CATH) 6 domains

SCOP 2.08 (2 domains)

CATH v4.4 (4 domains)

7. Citations (1)

CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA

1. Protein Identity and Related Structures Protein Identity & Related Structures

TYPE II RESTRICTION ENZYME NAEI

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1iaw__assembly_1__model_1

1iaw__assembly_1__model_1 | I(q)

1iaw__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

Entity 3 Entity 3 water

6. Fold Classification (SCOP + CATH) 6 domains

SCOP 2.08 (2 domains)

CATH v4.4 (4 domains)

7. Citations (1)