1jiw

Crystal structure of the APR-APRin complex

Method: X-RAY DIFFRACTION Dmax: 93.6 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 1jiw

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1jiw
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1jiw
沉积日期 deposition_date2001-07-03
结构标题 titleCrystal structure of the APR-APRin complex
关键词 keywordsPseudomonas aeruginosa alkaline protease inhibitor, HYDROLASE-HYDROLASE INHIBITOR COMPLEX; HYDROLASE/HYDROLASE INHIBITOR
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier27.16
回转半径 Rg (电子) rg_electron26.69
零角强度 I(0) i064794000.00
分子量 molecular_weight61123.0 kDa
排除体积 excluded_volume75485 ų
包络体积 envelope_volume89950 ų
水化壳体积 shell_volume29143 ų
包络直径 envelope_diameter96.7
壳层 Rg shell_rg32.87
包络 Rg envelope_rg27.05
形状 Rg shape_rg26.69
总 Rg total_rg27.31
总原子数 total_atoms4306
残基数 n_residues575
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax93.6
Rg (实空间) rg_real27.24
Rg 误差 (实空间) rg_real_error0.65
I(0) (实空间) i0_real6.4790e+07
I(0) 误差 (实空间) i0_real_error8.9760e+05
Rg (倒空间) rg_reciprocal27.22
I(0) (倒空间) i0_reciprocal64790000.0000
解质量估计 total_estimate0.8681
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary29.1
偏度 Skewness skewness0.446
峰度 Kurtosis kurtosis-0.223
角度范围 angular_range— – 0.2900 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha16500000.0000
实空间数据点数 n_real_points59
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.794; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.956; Smooth: 0.942

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (5)

6. 折叠分类 (SCOP + CATH) 6 domains

SCOP 2.08 (3 domains)

结构域编号 domain_idd1jiwi_
类 Class classb — All beta proteins
折叠类型 Fold foldb.61 — Streptavidin-like
超家族 Superfamily superfamilyb.61.2 — beta-Barrel protease inhibitors
家族 Family familyb.61.2.1 — Metalloprotease inhibitor
结构域编号 domain_idd1jiwp1
类 Class classb — All beta proteins
折叠类型 Fold foldb.80 — Single-stranded right-handed beta-helix
超家族 Superfamily superfamilyb.80.7 — beta-Roll
家族 Family familyb.80.7.1 — Serralysin-like metalloprotease, C-terminal domain
结构域编号 domain_idd1jiwp2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.92 — Zincin-like
超家族 Superfamily superfamilyd.92.1 — Metalloproteases ('zincins'), catalytic domain
家族 Family familyd.92.1.6 — Serralysin-like metalloprotease, catalytic (N-terminal) domain

CATH v4.4 (3 domains)

结构域编号 domain_id1jiwI00
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology128 — Lipocalin
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id1jiwP01
类 Class class2 — Mainly Beta
架构 Architecture architecture150 — 2 Solenoid
拓扑 Topology topology10 — Alkaline Protease, subunit P, domain 1
同源超家族 H-superfamily homologous superfamily10 — Serralysin-like metalloprotease, C-terminal
结构域编号 domain_id1jiwP02
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology390 — Collagenase (Catalytic Domain)
同源超家族 H-superfamily homologous superfamily10 — Collagenase (Catalytic Domain)

7. 引用文献 (1)

8. 文件与曲线 (10)