1jof

Neurospora crassa 3-carboxy-cis,cis-mucoante lactonizing enzyme

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

CARBOXY-CIS,CIS-MUCONATE CYCLASE

Neurospora crassa

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 4	SULFATE ION × 4 BETA-MERCAPTOETHANOL × 8 ;PIPERAZINE-N,N'-BIS(2-ETHANESULFONIC ACID) ; × 1 water × 4	Consistent with protein count
2	Protein homooligomer	Homooligomer	Protein 4	SULFATE ION × 4 BETA-MERCAPTOETHANOL × 8 ;PIPERAZINE-N,N'-BIS(2-ETHANESULFONIC ACID) ; × 1 water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CMLE_NEUCR
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–365； UniProt 1–365 Author chain B； PDBConstruct 1–365； UniProt 1–365 Author chain C； PDBConstruct 1–365； UniProt 1–365 Author chain D； PDBConstruct 1–365； UniProt 1–365 Author chain E； PDBConstruct 1–365； UniProt 1–365 Author chain F； PDBConstruct 1–365； UniProt 1–365 Author chain G； PDBConstruct 1–365； UniProt 1–365 Author chain H； PDBConstruct 1–365； UniProt 1–365

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	1jof
Deposition date deposition_date	2001-07-28
Structure title title	Neurospora crassa 3-carboxy-cis,cis-mucoante lactonizing enzyme
Keywords keywords	BETA-PROPELLER, HOMOTETRAMER, SEMET-PROTEIN, ISOMERASE; ISOMERASE
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1jof__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1jof__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

1jof__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	37.98 Å
Rg (electron density)	37.32 Å
Total Rg	37.63 Å
Atom count	11562
Residues	1420
Excluded volume	204910 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`1jof__assembly_1__model_1`	tetrameric （4）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`1jof__assembly_2__model_1`	tetrameric （4）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Description description	CARBOXY-CIS,CIS-MUCONATE CYCLASE
Molecular weight formula_weight	41715.4 kDa
EC 编号 ec_number	5.5.1.5
Source method src_method	man
Copy count entity_count	8

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E,F,G,H
Atom count atom_count	23837

PLHHLMIGTWTPPGAIFTVQFDDEKLTCKLIKRTEIPQDEPISWMTFDHERKNIYGAAMKKWSSFAVKSPTEIVHEASHPIGGHPRANDADTNTRAIFLLAAKQPPYAVYANPFYKFAGYGNVFSVSETGKLEKNVQNYEYQENTGIHGMVFDPTETYLYSADLTANKLWTHRKLASGEVELVGSVDAPDPGDHPRWVAMHPTGNYLYALMEAGNRICEYVIDPATHMPVYTHHSFPLIPPGIPDRDPETGKGLYRADVCALTFSGKYMFASSRANKFELQGYIAGFKLRDCGSIEKQLFLSPTPTSGGHSNAVSPCPWSDEWMAITDDQEGWLEIYRWKDEFLHRVARVRIPEPGFGMNAIWYD

Source Source

Source organism organism	Neurospora crassa
NCBI taxonomy ID ncbi_taxonomy_id	5141
Host organism host_organism	Escherichia coli

Entity 2 Entity 2 non-polymer

Description description	SULFATE ION
Molecular weight formula_weight	96.1 kDa
Source method src_method	syn
Copy count entity_count	8

Entity 3 Entity 3 non-polymer

Description description	BETA-MERCAPTOETHANOL
Molecular weight formula_weight	78.1 kDa
Source method src_method	syn
Copy count entity_count	16

Entity 4 Entity 4 non-polymer

Description description	;PIPERAZINE-N,N'-BIS(2-ETHANESULFONIC ACID) ;
Molecular weight formula_weight	302.4 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 5 Entity 5 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	713

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6. Fold Classification (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

Domain ID domain_id	d1jofa_
Class class	b — All beta proteins
Fold Fold fold	b.69 — 7-bladed beta-propeller
Superfamily Superfamily superfamily	b.69.10 — 3-carboxy-cis,cis-mucoante lactonizing enzyme
Family Family family	b.69.10.1 — 3-carboxy-cis,cis-mucoante lactonizing enzyme

Domain ID domain_id	d1jofb_
Class class	b — All beta proteins
Fold Fold fold	b.69 — 7-bladed beta-propeller
Superfamily Superfamily superfamily	b.69.10 — 3-carboxy-cis,cis-mucoante lactonizing enzyme
Family Family family	b.69.10.1 — 3-carboxy-cis,cis-mucoante lactonizing enzyme

Domain ID domain_id	d1jofc_
Class class	b — All beta proteins
Fold Fold fold	b.69 — 7-bladed beta-propeller
Superfamily Superfamily superfamily	b.69.10 — 3-carboxy-cis,cis-mucoante lactonizing enzyme
Family Family family	b.69.10.1 — 3-carboxy-cis,cis-mucoante lactonizing enzyme

Domain ID domain_id	d1jofd_
Class class	b — All beta proteins
Fold Fold fold	b.69 — 7-bladed beta-propeller
Superfamily Superfamily superfamily	b.69.10 — 3-carboxy-cis,cis-mucoante lactonizing enzyme
Family Family family	b.69.10.1 — 3-carboxy-cis,cis-mucoante lactonizing enzyme

Domain ID domain_id	d1jofe_
Class class	b — All beta proteins
Fold Fold fold	b.69 — 7-bladed beta-propeller
Superfamily Superfamily superfamily	b.69.10 — 3-carboxy-cis,cis-mucoante lactonizing enzyme
Family Family family	b.69.10.1 — 3-carboxy-cis,cis-mucoante lactonizing enzyme

Domain ID domain_id	d1joff_
Class class	b — All beta proteins
Fold Fold fold	b.69 — 7-bladed beta-propeller
Superfamily Superfamily superfamily	b.69.10 — 3-carboxy-cis,cis-mucoante lactonizing enzyme
Family Family family	b.69.10.1 — 3-carboxy-cis,cis-mucoante lactonizing enzyme

Domain ID domain_id	d1jofg_
Class class	b — All beta proteins
Fold Fold fold	b.69 — 7-bladed beta-propeller
Superfamily Superfamily superfamily	b.69.10 — 3-carboxy-cis,cis-mucoante lactonizing enzyme
Family Family family	b.69.10.1 — 3-carboxy-cis,cis-mucoante lactonizing enzyme

Domain ID domain_id	d1jofh_
Class class	b — All beta proteins
Fold Fold fold	b.69 — 7-bladed beta-propeller
Superfamily Superfamily superfamily	b.69.10 — 3-carboxy-cis,cis-mucoante lactonizing enzyme
Family Family family	b.69.10.1 — 3-carboxy-cis,cis-mucoante lactonizing enzyme

CATH v4.4 (8 domains)

Domain ID domain_id	1jofA00
Class class	2 — Mainly Beta
Architecture architecture	130 — 7 Propeller
Topology topology	10 — Methylamine Dehydrogenase; Chain H
Homologous superfamily homologous superfamily	10 — YVTN repeat-like/Quinoprotein amine dehydrogenase

Domain ID domain_id	1jofB00
Class class	2 — Mainly Beta
Architecture architecture	130 — 7 Propeller
Topology topology	10 — Methylamine Dehydrogenase; Chain H
Homologous superfamily homologous superfamily	10 — YVTN repeat-like/Quinoprotein amine dehydrogenase

Domain ID domain_id	1jofC00
Class class	2 — Mainly Beta
Architecture architecture	130 — 7 Propeller
Topology topology	10 — Methylamine Dehydrogenase; Chain H
Homologous superfamily homologous superfamily	10 — YVTN repeat-like/Quinoprotein amine dehydrogenase

Domain ID domain_id	1jofD00
Class class	2 — Mainly Beta
Architecture architecture	130 — 7 Propeller
Topology topology	10 — Methylamine Dehydrogenase; Chain H
Homologous superfamily homologous superfamily	10 — YVTN repeat-like/Quinoprotein amine dehydrogenase

Domain ID domain_id	1jofE00
Class class	2 — Mainly Beta
Architecture architecture	130 — 7 Propeller
Topology topology	10 — Methylamine Dehydrogenase; Chain H
Homologous superfamily homologous superfamily	10 — YVTN repeat-like/Quinoprotein amine dehydrogenase

Domain ID domain_id	1jofF00
Class class	2 — Mainly Beta
Architecture architecture	130 — 7 Propeller
Topology topology	10 — Methylamine Dehydrogenase; Chain H
Homologous superfamily homologous superfamily	10 — YVTN repeat-like/Quinoprotein amine dehydrogenase

Domain ID domain_id	1jofG00
Class class	2 — Mainly Beta
Architecture architecture	130 — 7 Propeller
Topology topology	10 — Methylamine Dehydrogenase; Chain H
Homologous superfamily homologous superfamily	10 — YVTN repeat-like/Quinoprotein amine dehydrogenase

Domain ID domain_id	1jofH00
Class class	2 — Mainly Beta
Architecture architecture	130 — 7 Propeller
Topology topology	10 — Methylamine Dehydrogenase; Chain H
Homologous superfamily homologous superfamily	10 — YVTN repeat-like/Quinoprotein amine dehydrogenase

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Unit-cell length a length_a	92.290 Å
Unit-cell length b length_b	160.600 Å
Unit-cell length c length_c	237.500 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	32

Space group space_group	P 21 21 21
Space-group number int_tables_number	19
Crystal system crystal_system	—

Resolution resolution	2.500 Å
Low-resolution limit resolution_low	20.000 Å
R factor r_factor	—
R_work r_work	0.2120
R_free r_free	0.2510

Neurospora crassa 3-carboxy-cis,cis-mucoante lactonizing enzyme

1. Protein Identity and Related Structures Protein Identity & Related Structures

CARBOXY-CIS,CIS-MUCONATE CYCLASE

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1jof__assembly_1__model_1

1jof__assembly_1__model_1 | I(q)

1jof__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 water

6. Fold Classification (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

CATH v4.4 (8 domains)

7. Citations (2)

Neurospora crassa 3-carboxy-cis,cis-mucoante lactonizing enzyme

1. Protein Identity and Related Structures Protein Identity & Related Structures

CARBOXY-CIS,CIS-MUCONATE CYCLASE

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1jof__assembly_1__model_1

1jof__assembly_1__model_1 | I(q)

1jof__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 water

6. Fold Classification (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

CATH v4.4 (8 domains)

7. Citations (2)