1jr3

Crystal Structure of the Processivity Clamp Loader Gamma Complex of E. coli DNA Polymerase III

Method: X-RAY DIFFRACTION D_max: 124.1 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

▼

1. 结构基本信息 1. Structure Basics

条目编号 entry_id	1jr3
沉积日期 deposition_date	2001-08-10
结构标题 title	Crystal Structure of the Processivity Clamp Loader Gamma Complex of E. coli DNA Polymerase III
关键词 keywords	DNA Polymerase, Processivity, Processivity clamp, clamp loader, AAA+ ATPASE, TRANSFERASE; TRANSFERASE
实验方法 method	X-RAY DIFFRACTION

▼

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	40.39 Å
回转半径 Rg (电子) rg_electron	39.39 Å
零角强度 I(0) i0	589361000.00
分子量 molecular_weight	197270.0 kDa
排除体积 excluded_volume	247070 Å³
包络体积 envelope_volume	352010 Å³
水化壳体积 shell_volume	72403 Å³
包络直径 envelope_diameter	122.2 Å
壳层 Rg shell_rg	47.34 Å
包络 Rg envelope_rg	37.91 Å
形状 Rg shape_rg	39.39 Å
总 Rg total_rg	39.84 Å
总原子数 total_atoms	13845
残基数 n_residues	1769
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

▼

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	124.1 Å
Rg (实空间) rg_real	40.17 Å
Rg 误差 (实空间) rg_real_error	1.00 Å
I(0) (实空间) i0_real	5.8940e+08
I(0) 误差 (实空间) i0_real_error	1.0590e+07
Rg (倒空间) rg_reciprocal	40.39 Å
I(0) (倒空间) i0_reciprocal	589500000.0000
解质量估计 total_estimate	0.9037
解质量评级 solution_quality	EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	54.1 Å
偏度 Skewness skewness	0.048
峰度 Kurtosis kurtosis	-0.588
角度范围 angular_range	— – 0.1950 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	71450000.0000
实空间数据点数 n_real_points	40
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.937; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.980; Smooth: 0.952

▶

4. 晶体学与实验 4. Crystallography & Experiment

▶

5. 实体与聚合体信息 (5)

Entity 1 polymer

描述 description	DNA polymerase III subunit gamma
分子量 formula_weight	41510.9 kDa
EC 编号 ec_number	2.7.7.7
来源方法 src_method	man
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B,C
原子数 atom_count	13845

MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFSGTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVEDTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQKLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDALSLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGIEWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLIGRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQ

来源 Source

来源生物 organism	Escherichia coli
NCBI 分类编号 ncbi_taxonomy_id	562
宿主生物 host_organism	Escherichia coli

Entity 2 polymer

描述 description	DNA polymerase III, delta subunit
分子量 formula_weight	38745.6 kDa
EC 编号 ec_number	2.7.7.7
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	D

MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAAQGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTGLLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNLELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWVDALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKHRVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHKPLADVFIDG

来源 Source

来源生物 organism	Escherichia coli
NCBI 分类编号 ncbi_taxonomy_id	562
宿主生物 host_organism	Escherichia coli

Entity 3 polymer

描述 description	;DNA polymerase III, delta' subunit ;
分子量 formula_weight	36980.5 kDa
EC 编号 ec_number	2.7.7.7
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E

MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYALSRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGVDAVREVTEKLNEHARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCRLHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAELANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL

来源 Source

来源生物 organism	Escherichia coli
NCBI 分类编号 ncbi_taxonomy_id	562
宿主生物 host_organism	Escherichia coli

Entity 4 non-polymer

描述 description	ZINC ION
分子量 formula_weight	65.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	4

Entity 5 non-polymer

描述 description	SULFATE ION
分子量 formula_weight	96.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	3

▼

6. 折叠分类 (SCOP + CATH) 25 domains

SCOP 2.08 (10 domains)

结构域编号 domain_id	d1jr3a1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.80 — post-AAA+ oligomerization domain-like
超家族 Superfamily superfamily	a.80.1 — post-AAA+ oligomerization domain-like
家族 Family family	a.80.1.1 — DNA polymerase III clamp loader subunits, C-terminal domain

结构域编号 domain_id	d1jr3a2
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.37 — P-loop containing nucleoside triphosphate hydrolases
超家族 Superfamily superfamily	c.37.1 — P-loop containing nucleoside triphosphate hydrolases
家族 Family family	c.37.1.20 — Extended AAA-ATPase domain

结构域编号 domain_id	d1jr3b1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.80 — post-AAA+ oligomerization domain-like
超家族 Superfamily superfamily	a.80.1 — post-AAA+ oligomerization domain-like
家族 Family family	a.80.1.1 — DNA polymerase III clamp loader subunits, C-terminal domain

结构域编号 domain_id	d1jr3b2
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.37 — P-loop containing nucleoside triphosphate hydrolases
超家族 Superfamily superfamily	c.37.1 — P-loop containing nucleoside triphosphate hydrolases
家族 Family family	c.37.1.20 — Extended AAA-ATPase domain

结构域编号 domain_id	d1jr3c1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.80 — post-AAA+ oligomerization domain-like
超家族 Superfamily superfamily	a.80.1 — post-AAA+ oligomerization domain-like
家族 Family family	a.80.1.1 — DNA polymerase III clamp loader subunits, C-terminal domain

结构域编号 domain_id	d1jr3c2
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.37 — P-loop containing nucleoside triphosphate hydrolases
超家族 Superfamily superfamily	c.37.1 — P-loop containing nucleoside triphosphate hydrolases
家族 Family family	c.37.1.20 — Extended AAA-ATPase domain

结构域编号 domain_id	d1jr3d1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.80 — post-AAA+ oligomerization domain-like
超家族 Superfamily superfamily	a.80.1 — post-AAA+ oligomerization domain-like
家族 Family family	a.80.1.1 — DNA polymerase III clamp loader subunits, C-terminal domain

结构域编号 domain_id	d1jr3d2
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.37 — P-loop containing nucleoside triphosphate hydrolases
超家族 Superfamily superfamily	c.37.1 — P-loop containing nucleoside triphosphate hydrolases
家族 Family family	c.37.1.20 — Extended AAA-ATPase domain

结构域编号 domain_id	d1jr3e1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.80 — post-AAA+ oligomerization domain-like
超家族 Superfamily superfamily	a.80.1 — post-AAA+ oligomerization domain-like
家族 Family family	a.80.1.1 — DNA polymerase III clamp loader subunits, C-terminal domain

结构域编号 domain_id	d1jr3e2
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.37 — P-loop containing nucleoside triphosphate hydrolases
超家族 Superfamily superfamily	c.37.1 — P-loop containing nucleoside triphosphate hydrolases
家族 Family family	c.37.1.20 — Extended AAA-ATPase domain

CATH v4.4 (15 domains)

结构域编号 domain_id	1jr3A01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	300 — P-loop containing nucleotide triphosphate hydrolases

结构域编号 domain_id	1jr3A02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	272 — Zinc Finger, Delta Prime; domain 3
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	1jr3A03
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	8 — Helicase, Ruva Protein; domain 3
同源超家族 H-superfamily homologous superfamily	60 —

结构域编号 domain_id	1jr3B01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	300 — P-loop containing nucleotide triphosphate hydrolases

结构域编号 domain_id	1jr3B02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	272 — Zinc Finger, Delta Prime; domain 3
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	1jr3B03
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	8 — Helicase, Ruva Protein; domain 3
同源超家族 H-superfamily homologous superfamily	60 —

结构域编号 domain_id	1jr3C01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	300 — P-loop containing nucleotide triphosphate hydrolases

结构域编号 domain_id	1jr3C02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	272 — Zinc Finger, Delta Prime; domain 3
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	1jr3C03
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	8 — Helicase, Ruva Protein; domain 3
同源超家族 H-superfamily homologous superfamily	60 —

结构域编号 domain_id	1jr3D01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	300 — P-loop containing nucleotide triphosphate hydrolases

结构域编号 domain_id	1jr3D02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	272 — Zinc Finger, Delta Prime; domain 3
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	1jr3D03
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	8 — Helicase, Ruva Protein; domain 3
同源超家族 H-superfamily homologous superfamily	60 —

结构域编号 domain_id	1jr3E01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	300 — P-loop containing nucleotide triphosphate hydrolases

结构域编号 domain_id	1jr3E02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	8 — Helicase, Ruva Protein; domain 3
同源超家族 H-superfamily homologous superfamily	10 — Ubiquitin-associated (UBA) domain

结构域编号 domain_id	1jr3E03
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	272 — Zinc Finger, Delta Prime; domain 3
同源超家族 H-superfamily homologous superfamily	10 —

▶

7. 引用文献 (1)

▶

晶胞边长 a length_a	95.698 Å
晶胞边长 b length_b	95.857 Å
晶胞边长 c length_c	285.410 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	12

空间群 space_group	P 21 21 21
空间群编号 int_tables_number	19
晶系 crystal_system	—

分辨率 resolution	2.700 Å
最低分辨率 resolution_low	500.000 Å
R 因子 r_factor	—
R_work r_work	0.2676
R_free r_free	0.3043

fit	/app/data/saxs_computation/jr/1jr3/1jr3.fit
pddf	/app/data/pr_computation/jr/1jr3/1jr3.pr.out
profile	/app/data/saxs_computation/jr/1jr3/1jr3.dat

Crystal Structure of the Processivity Clamp Loader Gamma Complex of E. coli DNA Polymerase III

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合体信息 (5)

Entity 1 polymer

聚合体 Polymer

来源 Source

Entity 2 polymer

聚合体 Polymer

来源 Source

Entity 3 polymer

聚合体 Polymer

来源 Source

Entity 4 non-polymer

Entity 5 non-polymer

6. 折叠分类 (SCOP + CATH) 25 domains

SCOP 2.08 (10 domains)

CATH v4.4 (15 domains)

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)