1kke

Crystal Structure of Reovirus Attachment Protein Sigma1 Trimer

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

SIGMA 1 PROTEIN

Mammalian orthoreovirus 3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 3	water × 3	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	VSI1_REOVD
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–210； UniProt 246–455 Author chain B； PDBConstruct 1–210； UniProt 246–455 Author chain C； PDBConstruct 1–210； UniProt 246–455

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	1kke
Deposition date deposition_date	2001-12-07
Structure title title	Crystal Structure of Reovirus Attachment Protein Sigma1 Trimer
Keywords keywords	reovirus, sigma1, fiber, beta-spiral, beta-barrel, trimer, receptor-binding, Viral protein; VIRAL PROTEIN
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1kke__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1kke__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

1kke__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	32.60 Å
Rg (electron density)	33.01 Å
Total Rg	33.46 Å
Atom count	4710
Residues	617
Excluded volume	83465 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`1kke__assembly_1__model_1`	trimeric （3）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (2)

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6. Fold Classification (SCOP + CATH) 12 domains

SCOP 2.08 (6 domains)

Domain ID domain_id	d1kkea1
Class class	b — All beta proteins
Fold Fold fold	b.83 — Triple beta-spiral
Superfamily Superfamily superfamily	b.83.1 — Fibre shaft of virus attachment proteins
Family Family family	b.83.1.2 — Reovirus attachment protein sigma 1

Domain ID domain_id	d1kkea2
Class class	b — All beta proteins
Fold Fold fold	b.21 — Virus attachment protein globular domain
Superfamily Superfamily superfamily	b.21.1 — Virus attachment protein globular domain
Family Family family	b.21.1.2 — Reovirus attachment protein sigma 1 head domain

Domain ID domain_id	d1kkeb1
Class class	b — All beta proteins
Fold Fold fold	b.83 — Triple beta-spiral
Superfamily Superfamily superfamily	b.83.1 — Fibre shaft of virus attachment proteins
Family Family family	b.83.1.2 — Reovirus attachment protein sigma 1

Domain ID domain_id	d1kkeb2
Class class	b — All beta proteins
Fold Fold fold	b.21 — Virus attachment protein globular domain
Superfamily Superfamily superfamily	b.21.1 — Virus attachment protein globular domain
Family Family family	b.21.1.2 — Reovirus attachment protein sigma 1 head domain

Domain ID domain_id	d1kkec1
Class class	b — All beta proteins
Fold Fold fold	b.83 — Triple beta-spiral
Superfamily Superfamily superfamily	b.83.1 — Fibre shaft of virus attachment proteins
Family Family family	b.83.1.2 — Reovirus attachment protein sigma 1

Domain ID domain_id	d1kkec2
Class class	b — All beta proteins
Fold Fold fold	b.21 — Virus attachment protein globular domain
Superfamily Superfamily superfamily	b.21.1 — Virus attachment protein globular domain
Family Family family	b.21.1.2 — Reovirus attachment protein sigma 1 head domain

CATH v4.4 (6 domains)

Domain ID domain_id	1kkeA01
Class class	2 — Mainly Beta
Architecture architecture	10 — Ribbon
Topology topology	25 — Laminin
Homologous superfamily homologous superfamily	20 — reovirus attachment protein sigma1; domain 1

Domain ID domain_id	1kkeA02
Class class	2 — Mainly Beta
Architecture architecture	60 — Sandwich
Topology topology	90 — Adenovirus Type 5 Fiber Protein (Receptor Binding Domain)
Homologous superfamily homologous superfamily	20 — Virus attachment protein , globular domain

Domain ID domain_id	1kkeB01
Class class	2 — Mainly Beta
Architecture architecture	10 — Ribbon
Topology topology	25 — Laminin
Homologous superfamily homologous superfamily	20 — reovirus attachment protein sigma1; domain 1

Domain ID domain_id	1kkeB02
Class class	2 — Mainly Beta
Architecture architecture	60 — Sandwich
Topology topology	90 — Adenovirus Type 5 Fiber Protein (Receptor Binding Domain)
Homologous superfamily homologous superfamily	20 — Virus attachment protein , globular domain

Domain ID domain_id	1kkeC01
Class class	2 — Mainly Beta
Architecture architecture	10 — Ribbon
Topology topology	25 — Laminin
Homologous superfamily homologous superfamily	20 — reovirus attachment protein sigma1; domain 1

Domain ID domain_id	1kkeC02
Class class	2 — Mainly Beta
Architecture architecture	60 — Sandwich
Topology topology	90 — Adenovirus Type 5 Fiber Protein (Receptor Binding Domain)
Homologous superfamily homologous superfamily	20 — Virus attachment protein , globular domain

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Unit-cell length a length_a	52.700 Å
Unit-cell length b length_b	89.000 Å
Unit-cell length c length_c	119.800 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	12

Space group space_group	P 21 21 21
Space-group number int_tables_number	19
Crystal system crystal_system	—

Resolution resolution	2.600 Å
Low-resolution limit resolution_low	20.000 Å
R factor r_factor	—
R_work r_work	0.1720
R_free r_free	0.2320

Description description	SIGMA 1 PROTEIN
Molecular weight formula_weight	22687.4 kDa
Source method src_method	man
Copy count entity_count	3

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C
Atom count atom_count	4899

Crystal Structure of Reovirus Attachment Protein Sigma1 Trimer

1. Protein Identity and Related Structures Protein Identity & Related Structures

SIGMA 1 PROTEIN

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1kke__assembly_1__model_1

1kke__assembly_1__model_1 | I(q)

1kke__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 water

6. Fold Classification (SCOP + CATH) 12 domains

SCOP 2.08 (6 domains)

CATH v4.4 (6 domains)

7. Citations (1)

Source organism organism	Mammalian orthoreovirus 3
NCBI taxonomy ID ncbi_taxonomy_id	10886
Host organism host_organism	Spodoptera frugiperda

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	189

Crystal Structure of Reovirus Attachment Protein Sigma1 Trimer

1. Protein Identity and Related Structures Protein Identity & Related Structures

SIGMA 1 PROTEIN

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1kke__assembly_1__model_1

1kke__assembly_1__model_1 | I(q)

1kke__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 water

6. Fold Classification (SCOP + CATH) 12 domains

SCOP 2.08 (6 domains)

CATH v4.4 (6 domains)

7. Citations (1)