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1lu1

THE STRUCTURE OF THE DOLICHOS BIFLORUS SEED LECTIN IN COMPLEX WITH THE FORSSMAN DISACCHARIDE

Method: X-RAY DIFFRACTION Dmax: 59.9 Å Quality: REASONABLE
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1. Protein Identity and Related Structures Protein Identity & Related Structures

LECTIN

Vigna unguiculata subsp. cylindrica

UniProt P05045

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Homooligomer Protein × 4 其他Polymer 4 PDB declaration: tetrameric（4） Consistent with protein copy count Chain A； UniProt 23–275 Not recorded 2-acetamido-2-deoxy-alpha-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-galactopyranose × 4 CA CALCIUM ION × 4 MN MANGANESE (II) ION × 4 ADE ADENINE × 4 X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；100 MM HEPES PH 7.5 0.2 M MGCL2 30% (W/V) PEG 400 Resolution 2.60 Å R-free 0.234

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 1LU1 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1BJQ THE DOLICHOS BIFLORUS SEED LECTIN IN COMPLEX WITH ADENINE Deposited 1998-06-26 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 23–275（253 aa）
Chain B 23–275（253 aa）
Chain C 23–275（253 aa）
Chain D 23–275（253 aa）
Not recorded CA CALCIUM ION × 4 MN MANGANESE (II) ION × 4 ADE ADENINE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；100 MM NA-CACODYLATE PH 6.5 10 % (W/V) PEG 8000 20 MM MGAC2 1 MM ADENINE
Resolution 2.65 Å R-free 0.246
1BJQ THE DOLICHOS BIFLORUS SEED LECTIN IN COMPLEX WITH ADENINE Deposited 1998-06-26 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain E 23–275（253 aa）
Chain F 23–275（253 aa）
Chain G 23–275（253 aa）
Chain H 23–275（253 aa）
Not recorded CA CALCIUM ION × 4 MN MANGANESE (II) ION × 4 ADE ADENINE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；100 MM NA-CACODYLATE PH 6.5 10 % (W/V) PEG 8000 20 MM MGAC2 1 MM ADENINE
Resolution 2.65 Å R-free 0.246
1LU2 DOLICHOS BIFLORUS SEED LECTIN IN COMPLEX WITH THE BLOOD GROUP A TRISACCHARIDE Deposited 1998-07-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 23–275（253 aa）
Chain B 23–275（253 aa）
Not recorded A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 4 CA CALCIUM ION × 4 MN MANGANESE (II) ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.6；100 MM NACACO PH 6.6 15% (W/V) PEG-ME 5000 10 MM BLOOD GROUP A TRISACCHARIDE
Resolution 2.80 Å R-free 0.219

2 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name LEC1_DOLBI
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–253； UniProt 23–275

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 1lu1

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 1lu1
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2. Structure Basics 2. Structure Basics

Entry ID entry_id1lu1
Deposition date deposition_date1998-07-24
Structure title titleTHE STRUCTURE OF THE DOLICHOS BIFLORUS SEED LECTIN IN COMPLEX WITH THE FORSSMAN DISACCHARIDE
Keywords keywordsLEGUME LECTINS, FORSSMAN DISACCHARIDE, DOLICHOS BIFLORUS SEED LECTIN, LECTIN; LECTIN
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier18.47 Å
Radius of gyration Rg (electron density) rg_electron17.13 Å
Forward intensity I(0) i013063500.00
Molecular weight molecular_weight27210.0 kDa
Excluded volume excluded_volume34007 ų
Envelope volume envelope_volume37822 ų
Hydration-shell volume shell_volume18252 ų
Envelope diameter envelope_diameter60.4 Å
Shell Rg shell_rg23.62 Å
Envelope Rg envelope_rg17.43 Å
Shape Rg shape_rg17.10 Å
Total Rg total_rg18.19 Å
Total atoms total_atoms1921
Residues n_residues253
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax59.9 Å
Rg (real space) rg_real18.67 Å
Rg uncertainty (real space) rg_real_error0.12 Å
I(0) (real space) i0_real1.2820e+07
I(0) uncertainty (real space) i0_real_error1.2850e+05
Rg (reciprocal space) rg_reciprocal18.38 Å
I(0) (reciprocal space) i0_reciprocal13060000.0000
Solution quality estimate total_estimate0.6750
Solution quality rating solution_quality REASONABLE a REASONABLE solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary24.6 Å
Skewness Skewness skewness0.292
Kurtosis Kurtosis kurtosis-0.069
Angular range angular_range— – 0.4300 Å−1
Current regularization parameter α current_alpha7.8110
Highest regularization parameter α highest_alpha3226000.0000
Real-space data points n_real_points74
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.847; Stabil: 0.931; Sysdev: 0.000; Positv: 1.000; Valcen: 0.995; Smooth: 0.471
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (5)

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7. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

Domain ID domain_idd1lu1a_
Class classb — All beta proteins
Fold Fold foldb.29 — Concanavalin A-like lectins/glucanases
Superfamily Superfamily superfamilyb.29.1 — Concanavalin A-like lectins/glucanases
Family Family familyb.29.1.1 — Legume lectins

CATH v4.4 (1 domains)

Domain ID domain_id1lu1A00
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology120 — Jelly Rolls
Homologous superfamily homologous superfamily200 —
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8. Citations (1)

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9. Files and Curves (10)