1ngm

Crystal structure of a yeast Brf1-TBP-DNA ternary complex

Method: X-RAY DIFFRACTION
▼

1. 蛋白身份与相关结构 Protein Identity & Related Structures

Transcription initiation factor TFIID

Saccharomyces cerevisiae

UniProt P13393

当前结构中的状态

Assembly 物理组成 蛋白状态 分子拷贝数 共同组分 数据核对
1 蛋白–DNA 异源复合物 蛋白 2 DNA 2 5'-D(*CP*TP*AP*TP*AP*AP*AP*AP*AP*AP*AP*TP*GP*TP*TP*TP*TP*TP*T)-3' × 1 5'-D(*AP*AP*AP*AP*AP*AP*CP*AP*TP*TP*TP*TP*TP*TP*TP*AP*TP*AP*G)-3' × 1 Transcription factor IIIB BRF1 subunit × 1 (P29056) 与全部聚合物一致
2 蛋白–DNA 异源复合物 蛋白 2 DNA 2 5'-D(*CP*TP*AP*TP*AP*AP*AP*AP*AP*AP*AP*TP*GP*TP*TP*TP*TP*TP*T)-3' × 1 5'-D(*AP*AP*AP*AP*AP*AP*CP*AP*TP*TP*TP*TP*TP*TP*TP*AP*TP*AP*G)-3' × 1 Transcription factor IIIB BRF1 subunit × 1 (P29056) 与全部聚合物一致
3 蛋白–DNA 异源复合物 蛋白 2 DNA 2 5'-D(*CP*TP*AP*TP*AP*AP*AP*AP*AP*AP*AP*TP*GP*TP*TP*TP*TP*TP*T)-3' × 1 5'-D(*AP*AP*AP*AP*AP*AP*CP*AP*TP*TP*TP*TP*TP*TP*TP*AP*TP*AP*G)-3' × 1 Transcription factor IIIB BRF1 subunit × 1 (P29056) 与全部聚合物一致
4 蛋白–DNA 异源复合物 蛋白 2 DNA 2 5'-D(*CP*TP*AP*TP*AP*AP*AP*AP*AP*AP*AP*TP*GP*TP*TP*TP*TP*TP*T)-3' × 1 5'-D(*AP*AP*AP*AP*AP*AP*CP*AP*TP*TP*TP*TP*TP*TP*TP*AP*TP*AP*G)-3' × 1 Transcription factor IIIB BRF1 subunit × 1 (P29056) 与全部聚合物一致

数据库中的同蛋白其他状态

查看构建体与数据证据
UniProt名称 TBP_YEAST
Isoform —
PDB实体 3
链与序列区间 作者链 A; PDB构建体 1–180; UniProt 60–239 作者链 E; PDB构建体 1–180; UniProt 60–239 作者链 I; PDB构建体 1–180; UniProt 60–239 作者链 M; PDB构建体 1–180; UniProt 60–239

Transcription factor IIIB BRF1 subunit

Saccharomyces cerevisiae

UniProt P29056

当前结构中的状态

Assembly 物理组成 蛋白状态 分子拷贝数 共同组分 数据核对
1 蛋白–DNA 异源复合物 蛋白 2 DNA 2 5'-D(*CP*TP*AP*TP*AP*AP*AP*AP*AP*AP*AP*TP*GP*TP*TP*TP*TP*TP*T)-3' × 1 5'-D(*AP*AP*AP*AP*AP*AP*CP*AP*TP*TP*TP*TP*TP*TP*TP*AP*TP*AP*G)-3' × 1 Transcription initiation factor TFIID × 1 (P13393) 与全部聚合物一致
2 蛋白–DNA 异源复合物 蛋白 2 DNA 2 5'-D(*CP*TP*AP*TP*AP*AP*AP*AP*AP*AP*AP*TP*GP*TP*TP*TP*TP*TP*T)-3' × 1 5'-D(*AP*AP*AP*AP*AP*AP*CP*AP*TP*TP*TP*TP*TP*TP*TP*AP*TP*AP*G)-3' × 1 Transcription initiation factor TFIID × 1 (P13393) 与全部聚合物一致
3 蛋白–DNA 异源复合物 蛋白 2 DNA 2 5'-D(*CP*TP*AP*TP*AP*AP*AP*AP*AP*AP*AP*TP*GP*TP*TP*TP*TP*TP*T)-3' × 1 5'-D(*AP*AP*AP*AP*AP*AP*CP*AP*TP*TP*TP*TP*TP*TP*TP*AP*TP*AP*G)-3' × 1 Transcription initiation factor TFIID × 1 (P13393) 与全部聚合物一致
4 蛋白–DNA 异源复合物 蛋白 2 DNA 2 5'-D(*CP*TP*AP*TP*AP*AP*AP*AP*AP*AP*AP*TP*GP*TP*TP*TP*TP*TP*T)-3' × 1 5'-D(*AP*AP*AP*AP*AP*AP*CP*AP*TP*TP*TP*TP*TP*TP*TP*AP*TP*AP*G)-3' × 1 Transcription initiation factor TFIID × 1 (P13393) 与全部聚合物一致

数据库中的同蛋白其他状态

查看构建体与数据证据
UniProt名称 TF3B_YEAST
Isoform —
PDB实体 4
链与序列区间 作者链 B; PDB构建体 3–72; UniProt 437–506 作者链 F; PDB构建体 3–72; UniProt 437–506 作者链 J; PDB构建体 3–72; UniProt 437–506 作者链 N; PDB构建体 3–72; UniProt 437–506

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。 链映射与序列区间收在“数据证据”中;数据库内部编号、导入时间和 assembly 操作表达式仅用于维护,因此不在读者页面展示。

▼

2. 结构基本信息 2. Structure Basics

条目编号 entry_id1ngm
沉积日期 deposition_date2002-12-17
结构标题 titleCrystal structure of a yeast Brf1-TBP-DNA ternary complex
关键词 keywordsTRANSCRIPTION, TFIIIB, TBP, BRF, BRF1, TAF, PROTEIN-DNA COMPLEX, TRANSCRIPTION-DNA COMPLEX; TRANSCRIPTION/DNA
实验方法 methodX-RAY DIFFRACTION
▼

3. Official assembly/model 理论 SAXS Official SAXS Profiles

页面只读取最新 assembly 计算结果。每一条曲线都对应明确的 PDB、official biological assembly 和 coordinate model。

1ngm__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

下载该曲线 .dat

1ngm__assembly_1__model_1 | I(q)

10-2 10-1 105 106 107 q (1/Angstrom) I(q)
100 个绘图点;横轴和纵轴均采用对数尺度。

1ngm__assembly_1__model_1 | P(r) · Pending

新版 assembly/model P(r) 尚未计算 此处仅保留位置,不展示旧数据 r (Angstrom) P(r)
P(r) 将按相同 assembly/model 分别计算和展示。
Rg(Guinier)24.39 Å
Rg(电子密度)23.77 Å
总 Rg24.52 Å
原子数2775
残基数290
排除体积47552 ų
最大 q0.500 Å⁻¹
Assembly Model 结构单元 聚集说明 状态 CRYSOL 操作
1 1 1ngm__assembly_1__model_1 tetrameric (4) 成功 4.1.3-1-20251215 (887e7ef) 查看 下载
2 1 1ngm__assembly_2__model_1 tetrameric (4) 成功 4.1.3-1-20251215 (887e7ef) 查看 下载
3 1 1ngm__assembly_3__model_1 tetrameric (4) 成功 4.1.3-1-20251215 (887e7ef) 查看 下载
4 1 1ngm__assembly_4__model_1 tetrameric (4) 成功 4.1.3-1-20251215 (887e7ef) 查看 下载
▶

4. 晶体学与实验 4. Crystallography & Experiment

▶

5. 实体与聚合物信息 Entities & Polymers (4)

▼

6. 折叠分类 (SCOP + CATH) 26 domains

SCOP 2.08 (14 domains)

结构域编号 domain_idd1ngma1
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.129 — TBP-like
超家族 Superfamily superfamilyd.129.1 — TATA-box binding protein-like
家族 Family familyd.129.1.1 — TATA-box binding protein (TBP), C-terminal domain
结构域编号 domain_idd1ngma2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.129 — TBP-like
超家族 Superfamily superfamilyd.129.1 — TATA-box binding protein-like
家族 Family familyd.129.1.1 — TATA-box binding protein (TBP), C-terminal domain
结构域编号 domain_idd1ngmb1
类 Class classj — Peptides
折叠类型 Fold foldj.104 — TBP-binding domain of the transcription factor IIIb Brf1 subunit
超家族 Superfamily superfamilyj.104.1 — TBP-binding domain of the transcription factor IIIb Brf1 subunit
家族 Family familyj.104.1.1 — TBP-binding domain of the transcription factor IIIb Brf1 subunit
结构域编号 domain_idd1ngmb2
类 Class classl — Artifacts
折叠类型 Fold foldl.1 — Tags
超家族 Superfamily superfamilyl.1.1 — Tags
家族 Family familyl.1.1.1 — Tags
结构域编号 domain_idd1ngme1
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.129 — TBP-like
超家族 Superfamily superfamilyd.129.1 — TATA-box binding protein-like
家族 Family familyd.129.1.1 — TATA-box binding protein (TBP), C-terminal domain
结构域编号 domain_idd1ngme2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.129 — TBP-like
超家族 Superfamily superfamilyd.129.1 — TATA-box binding protein-like
家族 Family familyd.129.1.1 — TATA-box binding protein (TBP), C-terminal domain
结构域编号 domain_idd1ngmf1
类 Class classj — Peptides
折叠类型 Fold foldj.104 — TBP-binding domain of the transcription factor IIIb Brf1 subunit
超家族 Superfamily superfamilyj.104.1 — TBP-binding domain of the transcription factor IIIb Brf1 subunit
家族 Family familyj.104.1.1 — TBP-binding domain of the transcription factor IIIb Brf1 subunit
结构域编号 domain_idd1ngmf2
类 Class classl — Artifacts
折叠类型 Fold foldl.1 — Tags
超家族 Superfamily superfamilyl.1.1 — Tags
家族 Family familyl.1.1.1 — Tags
结构域编号 domain_idd1ngmi1
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.129 — TBP-like
超家族 Superfamily superfamilyd.129.1 — TATA-box binding protein-like
家族 Family familyd.129.1.1 — TATA-box binding protein (TBP), C-terminal domain
结构域编号 domain_idd1ngmi2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.129 — TBP-like
超家族 Superfamily superfamilyd.129.1 — TATA-box binding protein-like
家族 Family familyd.129.1.1 — TATA-box binding protein (TBP), C-terminal domain
结构域编号 domain_idd1ngmj_
类 Class classj — Peptides
折叠类型 Fold foldj.104 — TBP-binding domain of the transcription factor IIIb Brf1 subunit
超家族 Superfamily superfamilyj.104.1 — TBP-binding domain of the transcription factor IIIb Brf1 subunit
家族 Family familyj.104.1.1 — TBP-binding domain of the transcription factor IIIb Brf1 subunit
结构域编号 domain_idd1ngmm1
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.129 — TBP-like
超家族 Superfamily superfamilyd.129.1 — TATA-box binding protein-like
家族 Family familyd.129.1.1 — TATA-box binding protein (TBP), C-terminal domain
结构域编号 domain_idd1ngmm2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.129 — TBP-like
超家族 Superfamily superfamilyd.129.1 — TATA-box binding protein-like
家族 Family familyd.129.1.1 — TATA-box binding protein (TBP), C-terminal domain
结构域编号 domain_idd1ngmn_
类 Class classj — Peptides
折叠类型 Fold foldj.104 — TBP-binding domain of the transcription factor IIIb Brf1 subunit
超家族 Superfamily superfamilyj.104.1 — TBP-binding domain of the transcription factor IIIb Brf1 subunit
家族 Family familyj.104.1.1 — TBP-binding domain of the transcription factor IIIb Brf1 subunit

CATH v4.4 (12 domains)

结构域编号 domain_id1ngmA01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology310 — TATA-Binding Protein
同源超家族 H-superfamily homologous superfamily10 — TATA-Binding Protein
结构域编号 domain_id1ngmA02
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology310 — TATA-Binding Protein
同源超家族 H-superfamily homologous superfamily10 — TATA-Binding Protein
结构域编号 domain_id1ngmB00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily650 — Single helix bin
结构域编号 domain_id1ngmE01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology310 — TATA-Binding Protein
同源超家族 H-superfamily homologous superfamily10 — TATA-Binding Protein
结构域编号 domain_id1ngmE02
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology310 — TATA-Binding Protein
同源超家族 H-superfamily homologous superfamily10 — TATA-Binding Protein
结构域编号 domain_id1ngmF00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily650 — Single helix bin
结构域编号 domain_id1ngmI01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology310 — TATA-Binding Protein
同源超家族 H-superfamily homologous superfamily10 — TATA-Binding Protein
结构域编号 domain_id1ngmI02
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology310 — TATA-Binding Protein
同源超家族 H-superfamily homologous superfamily10 — TATA-Binding Protein
结构域编号 domain_id1ngmJ00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily650 — Single helix bin
结构域编号 domain_id1ngmM01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology310 — TATA-Binding Protein
同源超家族 H-superfamily homologous superfamily10 — TATA-Binding Protein
结构域编号 domain_id1ngmM02
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology310 — TATA-Binding Protein
同源超家族 H-superfamily homologous superfamily10 — TATA-Binding Protein
结构域编号 domain_id1ngmN00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily650 — Single helix bin
▶

7. 引用文献 (1)