← Back to search
1owf

Crystal structure of a mutant IHF (BetaE44A) complexed with the native H' Site

Method: X-RAY DIFFRACTION
▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

Integration Host Factor Alpha-subunit

Escherichia coli

UniProt P0A6X7

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 2 DNA 3 ;Phage lambda H' site ; × 1 5'-D(*GP*GP*CP*CP*AP*AP*AP*AP*AP*AP*GP*CP*AP*TP*T)-3' × 1 5'-D(*GP*CP*TP*TP*AP*TP*CP*AP*AP*TP*TP*TP*GP*TP*TP*GP*CP*AP*CP*C)-3' × 1 Integration Host Factor beta-subunit × 1 （P0A6Y1） water × 5 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1ihf 1ouz 1owf 1owg 2ht0 2iie 2iif 9f0x 9f0y 9f0z 9f10 9f11

13 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name IHFA_ECOLI
Isoform —
PDB entities 4
Chains and sequence ranges Author chain A； PDBConstruct 1–99； UniProt 1–99

Integration Host Factor beta-subunit

Escherichia coli

UniProt P0A6Y1

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 2 DNA 3 ;Phage lambda H' site ; × 1 5'-D(*GP*GP*CP*CP*AP*AP*AP*AP*AP*AP*GP*CP*AP*TP*T)-3' × 1 5'-D(*GP*CP*TP*TP*AP*TP*CP*AP*AP*TP*TP*TP*GP*TP*TP*GP*CP*AP*CP*C)-3' × 1 Integration Host Factor Alpha-subunit × 1 （P0A6X7） water × 5 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1ihf 1ouz 1owf 1owg 2ht0 2iie 2iif 9f0x 9f0y 9f0z 9f10 9f11

13 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name IHFB_ECOLI
Isoform —
PDB entities 5
Chains and sequence ranges Author chain B； PDBConstruct 1–94； UniProt 1–94

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id1owf
Deposition date deposition_date2003-03-28
Structure title titleCrystal structure of a mutant IHF (BetaE44A) complexed with the native H' Site
Keywords keywordsprotein-DNA recognition, indirect readout, IHF, DNA bending, minor groove, TRANSCRIPTION-DNA COMPLEX; TRANSCRIPTION/DNA
Experimental Method methodX-RAY DIFFRACTION
▼

3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1owf__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1owf__assembly_1__model_1 | I(q)

10-2 10-1 106 107 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

1owf__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）23.70 Å
Rg (electron density)22.25 Å
Total Rg23.02 Å
Atom count2944
Residues260
Excluded volume47825 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 1owf__assembly_1__model_1 pentameric （5） Success 4.1.3-1-20251215 (887e7ef) View Download
▶

4. Crystallography and Experiment 4. Crystallography & Experiment

▶

5. Entities and Polymers Entities & Polymers (6)

▼

6. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

Domain ID domain_idd1owfa_
Class classa — All alpha proteins
Fold Fold folda.55 — IHF-like DNA-binding proteins
Superfamily Superfamily superfamilya.55.1 — IHF-like DNA-binding proteins
Family Family familya.55.1.1 — Prokaryotic DNA-bending protein
Domain ID domain_idd1owfb_
Class classa — All alpha proteins
Fold Fold folda.55 — IHF-like DNA-binding proteins
Superfamily Superfamily superfamilya.55.1 — IHF-like DNA-binding proteins
Family Family familya.55.1.1 — Prokaryotic DNA-bending protein

CATH v4.4 (2 domains)

Domain ID domain_id1owfA00
Class class4 — Few Secondary Structures
Architecture architecture10 — Irregular
Topology topology520 — HU Protein; Chain A
Homologous superfamily homologous superfamily10 — IHF-like DNA-binding proteins
Domain ID domain_id1owfB00
Class class4 — Few Secondary Structures
Architecture architecture10 — Irregular
Topology topology520 — HU Protein; Chain A
Homologous superfamily homologous superfamily10 — IHF-like DNA-binding proteins
▶

7. Citations (1)