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1q90

Structure of the cytochrome b6f (plastohydroquinone : plastocyanin oxidoreductase) from Chlamydomonas reinhardtii

Method: X-RAY DIFFRACTION Dmax: 124.2 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Apocytochrome f

Chlamydomonas reinhardtii

UniProt P23577

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 18 PDB declaration: octadecameric（18） Consistent with protein copy count Chain A； UniProt 32–317 Fragment：Residues 1-292 Mutation：6 His tag at C-terminus Cytochrome b6 × 2 （Q00471） Cytochrome B6-F complex iron-sulfur subunit × 2 （P49728） Cytochrome b6-f complex subunit 4 × 2 （Q42496） Cytochrome B6-F complex iron-sulfur subunit × 2 （P23230） Cytochrome b6f complex subunit petG × 2 （P49728） Cytochrome b6f complex subunit petL × 2 （P50369） Cytochrome b6f complex subunit PETM × 2 （Q08362） Cytochrome b6f complex subunit PETN × 2 （P50369） HEC HEME C × 8 BCR BETA-CAROTENE × 2 LFA EICOSANE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 CLA CHLOROPHYLL A × 2 TDS 8-HYDROXY-5,7-DIMETHOXY-3-METHYL-2-TRIDECYL-4H-CHROMEN-4-ONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；277 K；Reservoir: 25% PEG-MME 350, 40 millimolar TRIS HCL PH 8, 40 millimolar nacl, 0.2 millimolar laurylmaltoside, 30% glycerol. Drop: 1.3 microliter protein + 0.7 microliter reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 277K Resolution 3.10 Å R-free 0.261
2 Protein heterocomplex Heteromer Protein × 36 PDB declaration: 36-meric（36） Consistent with protein copy count Chain A； UniProt 32–317 Fragment：Residues 1-292 Mutation：6 His tag at C-terminus Cytochrome b6 × 4 （Q00471） Cytochrome B6-F complex iron-sulfur subunit × 4 （P49728） Cytochrome b6-f complex subunit 4 × 4 （Q42496） Cytochrome B6-F complex iron-sulfur subunit × 4 （P23230） Cytochrome b6f complex subunit petG × 4 （P49728） Cytochrome b6f complex subunit petL × 4 （P50369） Cytochrome b6f complex subunit PETM × 4 （Q08362） Cytochrome b6f complex subunit PETN × 4 （P50369） HEC HEME C × 16 BCR BETA-CAROTENE × 4 LFA EICOSANE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 4 CLA CHLOROPHYLL A × 4 TDS 8-HYDROXY-5,7-DIMETHOXY-3-METHYL-2-TRIDECYL-4H-CHROMEN-4-ONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；277 K；Reservoir: 25% PEG-MME 350, 40 millimolar TRIS HCL PH 8, 40 millimolar nacl, 0.2 millimolar laurylmaltoside, 30% glycerol. Drop: 1.3 microliter protein + 0.7 microliter reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 277K Resolution 3.10 Å R-free 0.261

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 1Q90 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1CFM CYTOCHROME F FROM CHLAMYDOMONAS REINHARDTII Deposited 1998-09-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 32–282（251 aa）
Chain B 32–282（251 aa）
Chain C 32–282（251 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；pH 6.5
Resolution 2.00 Å R-free 0.271
1E2V N153Q mutant of cytochrome f from Chlamydomonas reinhardtii Deposited 2000-05-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 32–282（251 aa）
Mutation：YES HEC HEME C × 1 ACT ACETATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；THE PROTEIN WAS BUFFERED IN 10 MM NA2HPO4/NAH2PO4, 1 MM DTT, PH 6.5 AND THE RESERVOIR CONTAINED 100 MM MES, PH 6.5, 25 MM CALCIUM ACETATE, 1 MM DTT AND 17-19% PEG 8000.
Resolution 1.85 Å R-free 0.219
1E2V N153Q mutant of cytochrome f from Chlamydomonas reinhardtii Deposited 2000-05-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 32–282（251 aa）
Mutation：YES HEC HEME C × 1 ACT ACETATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；THE PROTEIN WAS BUFFERED IN 10 MM NA2HPO4/NAH2PO4, 1 MM DTT, PH 6.5 AND THE RESERVOIR CONTAINED 100 MM MES, PH 6.5, 25 MM CALCIUM ACETATE, 1 MM DTT AND 17-19% PEG 8000.
Resolution 1.85 Å R-free 0.219
1E2V N153Q mutant of cytochrome f from Chlamydomonas reinhardtii Deposited 2000-05-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 32–282（251 aa）
Mutation：YES HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；THE PROTEIN WAS BUFFERED IN 10 MM NA2HPO4/NAH2PO4, 1 MM DTT, PH 6.5 AND THE RESERVOIR CONTAINED 100 MM MES, PH 6.5, 25 MM CALCIUM ACETATE, 1 MM DTT AND 17-19% PEG 8000.
Resolution 1.85 Å R-free 0.219
1E2W N168F mutant of cytochrome f from Chlamydomonas reinhardtii Deposited 2000-05-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 32–282（251 aa）
Mutation：YES HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.7；THE PROTEIN WAS BUFFERED IN 10 MM NA2HPO4/NAH2PO4, PH 7.5, 1 MM DTT, THE RESERVOIR CONTAINED 100 MM MES, PH 6.7, 200 MM AMMONIUM FORMATE, 14% GLYCEROL, 17% PEG-3350.
Resolution 1.60 Å R-free 0.226
1E2W N168F mutant of cytochrome f from Chlamydomonas reinhardtii Deposited 2000-05-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 32–282（251 aa）
Mutation：YES HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.7；THE PROTEIN WAS BUFFERED IN 10 MM NA2HPO4/NAH2PO4, PH 7.5, 1 MM DTT, THE RESERVOIR CONTAINED 100 MM MES, PH 6.7, 200 MM AMMONIUM FORMATE, 14% GLYCEROL, 17% PEG-3350.
Resolution 1.60 Å R-free 0.226
1E2Z Q158L mutant of cytochrome f from Chlamydomonas reinhardtii Deposited 2000-05-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 32–282（251 aa）
Mutation：YES HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.7；THE PROTEIN WAS BUFFERED IN 10 MM NA2HPO4/NAH2PO4, 1 MM DTT, PH 7.5 AND THE RESERVOIR CONTAINED 100 MM MES, PH 6.7, 50 MM AMMONIUM FLUORIDE 5% GLYCEROL AND 19-21% PEG 3350.
Resolution 2.50 Å R-free 0.279
1E2Z Q158L mutant of cytochrome f from Chlamydomonas reinhardtii Deposited 2000-05-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 32–282（251 aa）
Mutation：YES HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.7；THE PROTEIN WAS BUFFERED IN 10 MM NA2HPO4/NAH2PO4, 1 MM DTT, PH 7.5 AND THE RESERVOIR CONTAINED 100 MM MES, PH 6.7, 50 MM AMMONIUM FLUORIDE 5% GLYCEROL AND 19-21% PEG 3350.
Resolution 2.50 Å R-free 0.279
1E2Z Q158L mutant of cytochrome f from Chlamydomonas reinhardtii Deposited 2000-05-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 32–282（251 aa）
Mutation：YES HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.7；THE PROTEIN WAS BUFFERED IN 10 MM NA2HPO4/NAH2PO4, 1 MM DTT, PH 7.5 AND THE RESERVOIR CONTAINED 100 MM MES, PH 6.7, 50 MM AMMONIUM FLUORIDE 5% GLYCEROL AND 19-21% PEG 3350.
Resolution 2.50 Å R-free 0.279
1EWH STRUCTURE OF CYTOCHROME F FROM CHLAMYDOMONAS REINHARDTII Deposited 2000-04-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 32–282（251 aa） Fragment：N-TERMINAL SOLUBLE FRAGMENT
Not recorded HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.5；293 K；PEG 8000, calcium acetate, MES, pH 6.5, VAPOR DIFFUSION, temperature 20K
Resolution 2.35 Å R-free 0.256
1EWH STRUCTURE OF CYTOCHROME F FROM CHLAMYDOMONAS REINHARDTII Deposited 2000-04-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 32–282（251 aa） Fragment：N-TERMINAL SOLUBLE FRAGMENT
Not recorded HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.5；293 K；PEG 8000, calcium acetate, MES, pH 6.5, VAPOR DIFFUSION, temperature 20K
Resolution 2.35 Å R-free 0.256
1EWH STRUCTURE OF CYTOCHROME F FROM CHLAMYDOMONAS REINHARDTII Deposited 2000-04-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 32–282（251 aa） Fragment：N-TERMINAL SOLUBLE FRAGMENT
Not recorded HEC HEME C × 1 ACT ACETATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.5；293 K；PEG 8000, calcium acetate, MES, pH 6.5, VAPOR DIFFUSION, temperature 20K
Resolution 2.35 Å R-free 0.256

5 other PDB entries and 12 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CYF_CHLRE
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–286； UniProt 32–317

Cytochrome b6

Chlamydomonas reinhardtii

UniProt Q00471

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 18 PDB declaration: octadecameric（18） Consistent with protein copy count Chain B； UniProt 1–215 Fragment：Residues 4-215 Apocytochrome f × 2 （P23577） Cytochrome B6-F complex iron-sulfur subunit × 2 （P49728） Cytochrome b6-f complex subunit 4 × 2 （Q42496） Cytochrome B6-F complex iron-sulfur subunit × 2 （P23230） Cytochrome b6f complex subunit petG × 2 （P49728） Cytochrome b6f complex subunit petL × 2 （P50369） Cytochrome b6f complex subunit PETM × 2 （Q08362） Cytochrome b6f complex subunit PETN × 2 （P50369） HEC HEME C × 8 BCR BETA-CAROTENE × 2 LFA EICOSANE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 CLA CHLOROPHYLL A × 2 TDS 8-HYDROXY-5,7-DIMETHOXY-3-METHYL-2-TRIDECYL-4H-CHROMEN-4-ONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；277 K；Reservoir: 25% PEG-MME 350, 40 millimolar TRIS HCL PH 8, 40 millimolar nacl, 0.2 millimolar laurylmaltoside, 30% glycerol. Drop: 1.3 microliter protein + 0.7 microliter reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 277K Resolution 3.10 Å R-free 0.261
2 Protein heterocomplex Heteromer Protein × 36 PDB declaration: 36-meric（36） Consistent with protein copy count Chain B； UniProt 1–215 Fragment：Residues 4-215 Apocytochrome f × 4 （P23577） Cytochrome B6-F complex iron-sulfur subunit × 4 （P49728） Cytochrome b6-f complex subunit 4 × 4 （Q42496） Cytochrome B6-F complex iron-sulfur subunit × 4 （P23230） Cytochrome b6f complex subunit petG × 4 （P49728） Cytochrome b6f complex subunit petL × 4 （P50369） Cytochrome b6f complex subunit PETM × 4 （Q08362） Cytochrome b6f complex subunit PETN × 4 （P50369） HEC HEME C × 16 BCR BETA-CAROTENE × 4 LFA EICOSANE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 4 CLA CHLOROPHYLL A × 4 TDS 8-HYDROXY-5,7-DIMETHOXY-3-METHYL-2-TRIDECYL-4H-CHROMEN-4-ONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；277 K；Reservoir: 25% PEG-MME 350, 40 millimolar TRIS HCL PH 8, 40 millimolar nacl, 0.2 millimolar laurylmaltoside, 30% glycerol. Drop: 1.3 microliter protein + 0.7 microliter reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 277K Resolution 3.10 Å R-free 0.261

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence
UniProt name CYB6_CHLRE
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–215； UniProt 1–215

Cytochrome B6-F complex iron-sulfur subunit

Chlamydomonas reinhardtii

UniProt P49728

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 18 PDB declaration: octadecameric（18） Consistent with protein copy count Chain C； UniProt 80–206 Chain G； UniProt 1–37 Fragment：Soluble domain Fragment：Residues 1-30 Apocytochrome f × 2 （P23577） Cytochrome b6 × 2 （Q00471） Cytochrome b6-f complex subunit 4 × 2 （Q42496） Cytochrome B6-F complex iron-sulfur subunit × 2 （P23230） Cytochrome b6f complex subunit petL × 2 （P50369） Cytochrome b6f complex subunit PETM × 2 （Q08362） Cytochrome b6f complex subunit PETN × 2 （P50369） HEC HEME C × 8 BCR BETA-CAROTENE × 2 LFA EICOSANE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 CLA CHLOROPHYLL A × 2 TDS 8-HYDROXY-5,7-DIMETHOXY-3-METHYL-2-TRIDECYL-4H-CHROMEN-4-ONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；277 K；Reservoir: 25% PEG-MME 350, 40 millimolar TRIS HCL PH 8, 40 millimolar nacl, 0.2 millimolar laurylmaltoside, 30% glycerol. Drop: 1.3 microliter protein + 0.7 microliter reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 277K Resolution 3.10 Å R-free 0.261
2 Protein heterocomplex Heteromer Protein × 36 PDB declaration: 36-meric（36） Consistent with protein copy count Chain C； UniProt 80–206 Chain G； UniProt 1–37 Fragment：Soluble domain Fragment：Residues 1-30 Apocytochrome f × 4 （P23577） Cytochrome b6 × 4 （Q00471） Cytochrome b6-f complex subunit 4 × 4 （Q42496） Cytochrome B6-F complex iron-sulfur subunit × 4 （P23230） Cytochrome b6f complex subunit petL × 4 （P50369） Cytochrome b6f complex subunit PETM × 4 （Q08362） Cytochrome b6f complex subunit PETN × 4 （P50369） HEC HEME C × 16 BCR BETA-CAROTENE × 4 LFA EICOSANE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 4 CLA CHLOROPHYLL A × 4 TDS 8-HYDROXY-5,7-DIMETHOXY-3-METHYL-2-TRIDECYL-4H-CHROMEN-4-ONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；277 K；Reservoir: 25% PEG-MME 350, 40 millimolar TRIS HCL PH 8, 40 millimolar nacl, 0.2 millimolar laurylmaltoside, 30% glycerol. Drop: 1.3 microliter protein + 0.7 microliter reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 277K Resolution 3.10 Å R-free 0.261

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence
UniProt name UCRIA_CHLRE
Isoform —
PDB entities 3, 6
Chains and sequence ranges Author chain C； PDBConstruct 1–127； UniProt 80–206 Author chain G； PDBConstruct 1–37； UniProt 1–37

Cytochrome b6-f complex subunit 4

Chlamydomonas reinhardtii

UniProt Q42496

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 18 PDB declaration: octadecameric（18） Consistent with protein copy count Chain D； UniProt 1–159 Fragment：Residues 4-159 Apocytochrome f × 2 （P23577） Cytochrome b6 × 2 （Q00471） Cytochrome B6-F complex iron-sulfur subunit × 2 （P49728） Cytochrome B6-F complex iron-sulfur subunit × 2 （P23230） Cytochrome b6f complex subunit petG × 2 （P49728） Cytochrome b6f complex subunit petL × 2 （P50369） Cytochrome b6f complex subunit PETM × 2 （Q08362） Cytochrome b6f complex subunit PETN × 2 （P50369） HEC HEME C × 8 BCR BETA-CAROTENE × 2 LFA EICOSANE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 CLA CHLOROPHYLL A × 2 TDS 8-HYDROXY-5,7-DIMETHOXY-3-METHYL-2-TRIDECYL-4H-CHROMEN-4-ONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；277 K；Reservoir: 25% PEG-MME 350, 40 millimolar TRIS HCL PH 8, 40 millimolar nacl, 0.2 millimolar laurylmaltoside, 30% glycerol. Drop: 1.3 microliter protein + 0.7 microliter reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 277K Resolution 3.10 Å R-free 0.261
2 Protein heterocomplex Heteromer Protein × 36 PDB declaration: 36-meric（36） Consistent with protein copy count Chain D； UniProt 1–159 Fragment：Residues 4-159 Apocytochrome f × 4 （P23577） Cytochrome b6 × 4 （Q00471） Cytochrome B6-F complex iron-sulfur subunit × 4 （P49728） Cytochrome B6-F complex iron-sulfur subunit × 4 （P23230） Cytochrome b6f complex subunit petG × 4 （P49728） Cytochrome b6f complex subunit petL × 4 （P50369） Cytochrome b6f complex subunit PETM × 4 （Q08362） Cytochrome b6f complex subunit PETN × 4 （P50369） HEC HEME C × 16 BCR BETA-CAROTENE × 4 LFA EICOSANE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 4 CLA CHLOROPHYLL A × 4 TDS 8-HYDROXY-5,7-DIMETHOXY-3-METHYL-2-TRIDECYL-4H-CHROMEN-4-ONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；277 K；Reservoir: 25% PEG-MME 350, 40 millimolar TRIS HCL PH 8, 40 millimolar nacl, 0.2 millimolar laurylmaltoside, 30% glycerol. Drop: 1.3 microliter protein + 0.7 microliter reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 277K Resolution 3.10 Å R-free 0.261

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence
UniProt name PETM_CHLRE
Isoform —
PDB entities 4
Chains and sequence ranges Author chain D； PDBConstruct 1–159； UniProt 1–159

Cytochrome B6-F complex iron-sulfur subunit

Chlamydomonas reinhardtii

UniProt P23230

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 18 PDB declaration: octadecameric（18） Consistent with protein copy count Chain R； UniProt 31–79 Fragment：Transmembrane domain Apocytochrome f × 2 （P23577） Cytochrome b6 × 2 （Q00471） Cytochrome B6-F complex iron-sulfur subunit × 2 （P49728） Cytochrome b6-f complex subunit 4 × 2 （Q42496） Cytochrome b6f complex subunit petG × 2 （P49728） Cytochrome b6f complex subunit petL × 2 （P50369） Cytochrome b6f complex subunit PETM × 2 （Q08362） Cytochrome b6f complex subunit PETN × 2 （P50369） HEC HEME C × 8 BCR BETA-CAROTENE × 2 LFA EICOSANE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 CLA CHLOROPHYLL A × 2 TDS 8-HYDROXY-5,7-DIMETHOXY-3-METHYL-2-TRIDECYL-4H-CHROMEN-4-ONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；277 K；Reservoir: 25% PEG-MME 350, 40 millimolar TRIS HCL PH 8, 40 millimolar nacl, 0.2 millimolar laurylmaltoside, 30% glycerol. Drop: 1.3 microliter protein + 0.7 microliter reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 277K Resolution 3.10 Å R-free 0.261
2 Protein heterocomplex Heteromer Protein × 36 PDB declaration: 36-meric（36） Consistent with protein copy count Chain R； UniProt 31–79 Fragment：Transmembrane domain Apocytochrome f × 4 （P23577） Cytochrome b6 × 4 （Q00471） Cytochrome B6-F complex iron-sulfur subunit × 4 （P49728） Cytochrome b6-f complex subunit 4 × 4 （Q42496） Cytochrome b6f complex subunit petG × 4 （P49728） Cytochrome b6f complex subunit petL × 4 （P50369） Cytochrome b6f complex subunit PETM × 4 （Q08362） Cytochrome b6f complex subunit PETN × 4 （P50369） HEC HEME C × 16 BCR BETA-CAROTENE × 4 LFA EICOSANE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 4 CLA CHLOROPHYLL A × 4 TDS 8-HYDROXY-5,7-DIMETHOXY-3-METHYL-2-TRIDECYL-4H-CHROMEN-4-ONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；277 K；Reservoir: 25% PEG-MME 350, 40 millimolar TRIS HCL PH 8, 40 millimolar nacl, 0.2 millimolar laurylmaltoside, 30% glycerol. Drop: 1.3 microliter protein + 0.7 microliter reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 277K Resolution 3.10 Å R-free 0.261

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence
UniProt name PETD_CHLRE
Isoform —
PDB entities 5
Chains and sequence ranges Author chain R； PDBConstruct 1–49； UniProt 31–79

Cytochrome b6f complex subunit petL

Chlamydomonas reinhardtii

UniProt P50369

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 18 PDB declaration: octadecameric（18） Consistent with protein copy count Chain L； UniProt 12–43 Chain N； UniProt 68–98 Fragment：Residues 1-32 Fragment：Residues 68-98 Apocytochrome f × 2 （P23577） Cytochrome b6 × 2 （Q00471） Cytochrome B6-F complex iron-sulfur subunit × 2 （P49728） Cytochrome b6-f complex subunit 4 × 2 （Q42496） Cytochrome B6-F complex iron-sulfur subunit × 2 （P23230） Cytochrome b6f complex subunit petG × 2 （P49728） Cytochrome b6f complex subunit PETM × 2 （Q08362） HEC HEME C × 8 BCR BETA-CAROTENE × 2 LFA EICOSANE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 CLA CHLOROPHYLL A × 2 TDS 8-HYDROXY-5,7-DIMETHOXY-3-METHYL-2-TRIDECYL-4H-CHROMEN-4-ONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；277 K；Reservoir: 25% PEG-MME 350, 40 millimolar TRIS HCL PH 8, 40 millimolar nacl, 0.2 millimolar laurylmaltoside, 30% glycerol. Drop: 1.3 microliter protein + 0.7 microliter reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 277K Resolution 3.10 Å R-free 0.261
2 Protein heterocomplex Heteromer Protein × 36 PDB declaration: 36-meric（36） Consistent with protein copy count Chain L； UniProt 12–43 Chain N； UniProt 68–98 Fragment：Residues 1-32 Fragment：Residues 68-98 Apocytochrome f × 4 （P23577） Cytochrome b6 × 4 （Q00471） Cytochrome B6-F complex iron-sulfur subunit × 4 （P49728） Cytochrome b6-f complex subunit 4 × 4 （Q42496） Cytochrome B6-F complex iron-sulfur subunit × 4 （P23230） Cytochrome b6f complex subunit petG × 4 （P49728） Cytochrome b6f complex subunit PETM × 4 （Q08362） HEC HEME C × 16 BCR BETA-CAROTENE × 4 LFA EICOSANE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 4 CLA CHLOROPHYLL A × 4 TDS 8-HYDROXY-5,7-DIMETHOXY-3-METHYL-2-TRIDECYL-4H-CHROMEN-4-ONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；277 K；Reservoir: 25% PEG-MME 350, 40 millimolar TRIS HCL PH 8, 40 millimolar nacl, 0.2 millimolar laurylmaltoside, 30% glycerol. Drop: 1.3 microliter protein + 0.7 microliter reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 277K Resolution 3.10 Å R-free 0.261

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence
UniProt name PETL_CHLRE
Isoform —
PDB entities 7, 9
Chains and sequence ranges Author chain L； PDBConstruct 1–32； UniProt 12–43 Author chain N； PDBConstruct 1–31； UniProt 68–98

Cytochrome b6f complex subunit PETM

Chlamydomonas reinhardtii

UniProt Q08362

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 18 PDB declaration: octadecameric（18） Consistent with protein copy count Chain M； UniProt 61–99 Fragment：Residues 62-95 Apocytochrome f × 2 （P23577） Cytochrome b6 × 2 （Q00471） Cytochrome B6-F complex iron-sulfur subunit × 2 （P49728） Cytochrome b6-f complex subunit 4 × 2 （Q42496） Cytochrome B6-F complex iron-sulfur subunit × 2 （P23230） Cytochrome b6f complex subunit petG × 2 （P49728） Cytochrome b6f complex subunit petL × 2 （P50369） Cytochrome b6f complex subunit PETN × 2 （P50369） HEC HEME C × 8 BCR BETA-CAROTENE × 2 LFA EICOSANE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 CLA CHLOROPHYLL A × 2 TDS 8-HYDROXY-5,7-DIMETHOXY-3-METHYL-2-TRIDECYL-4H-CHROMEN-4-ONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；277 K；Reservoir: 25% PEG-MME 350, 40 millimolar TRIS HCL PH 8, 40 millimolar nacl, 0.2 millimolar laurylmaltoside, 30% glycerol. Drop: 1.3 microliter protein + 0.7 microliter reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 277K Resolution 3.10 Å R-free 0.261
2 Protein heterocomplex Heteromer Protein × 36 PDB declaration: 36-meric（36） Consistent with protein copy count Chain M； UniProt 61–99 Fragment：Residues 62-95 Apocytochrome f × 4 （P23577） Cytochrome b6 × 4 （Q00471） Cytochrome B6-F complex iron-sulfur subunit × 4 （P49728） Cytochrome b6-f complex subunit 4 × 4 （Q42496） Cytochrome B6-F complex iron-sulfur subunit × 4 （P23230） Cytochrome b6f complex subunit petG × 4 （P49728） Cytochrome b6f complex subunit petL × 4 （P50369） Cytochrome b6f complex subunit PETN × 4 （P50369） HEC HEME C × 16 BCR BETA-CAROTENE × 4 LFA EICOSANE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 4 CLA CHLOROPHYLL A × 4 TDS 8-HYDROXY-5,7-DIMETHOXY-3-METHYL-2-TRIDECYL-4H-CHROMEN-4-ONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；277 K；Reservoir: 25% PEG-MME 350, 40 millimolar TRIS HCL PH 8, 40 millimolar nacl, 0.2 millimolar laurylmaltoside, 30% glycerol. Drop: 1.3 microliter protein + 0.7 microliter reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 277K Resolution 3.10 Å R-free 0.261

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence
UniProt name PETG_CHLRE
Isoform —
PDB entities 8
Chains and sequence ranges Author chain M； PDBConstruct 1–39； UniProt 61–99

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 1q90

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 1q90
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2. Structure Basics 2. Structure Basics

Entry ID entry_id1q90
Deposition date deposition_date2003-08-22
Structure title titleStructure of the cytochrome b6f (plastohydroquinone : plastocyanin oxidoreductase) from Chlamydomonas reinhardtii
Keywords keywords;MEMBRANE PROTEIN COMPLEX, PHOTOSYNTHESIS, ELECTRON TRANSFER, OXYDOREDUCTASE, CHLOROPHYLL, BETA-CAROTENE, STIGMATELLIN, SULFOQUINOVOSYLDIACYLGLYCEROL, MONOGALACTOSYLDIACYLGLYCEROL ;; PHOTOSYNTHESIS
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier34.03 Å
Radius of gyration Rg (electron density) rg_electron33.02 Å
Forward intensity I(0) i0153267000.00
Molecular weight molecular_weight110360.0 kDa
Excluded volume excluded_volume142700 ų
Envelope volume envelope_volume176790 ų
Hydration-shell volume shell_volume45004 ų
Envelope diameter envelope_diameter131.0 Å
Shell Rg shell_rg39.32 Å
Envelope Rg envelope_rg33.86 Å
Shape Rg shape_rg33.04 Å
Total Rg total_rg33.46 Å
Total atoms total_atoms7776
Residues n_residues952
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax124.2 Å
Rg (real space) rg_real34.19 Å
Rg uncertainty (real space) rg_real_error1.08 Å
I(0) (real space) i0_real1.5330e+08
I(0) uncertainty (real space) i0_real_error2.3880e+06
Rg (reciprocal space) rg_reciprocal34.09 Å
I(0) (reciprocal space) i0_reciprocal153300000.0000
Solution quality estimate total_estimate0.8221
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary37.0 Å
Skewness Skewness skewness0.556
Kurtosis Kurtosis kurtosis0.018
Angular range angular_range— – 0.2350 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha20300000.0000
Real-space data points n_real_points48
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.642; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.832; Smooth: 0.925
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (18)

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7. Fold Classification (SCOP + CATH) 18 domains

SCOP 2.08 (12 domains)

Domain ID domain_idd1q90a1
Class classb — All beta proteins
Fold Fold foldb.2 — Common fold of diphtheria toxin/transcription factors/cytochrome f
Superfamily Superfamily superfamilyb.2.6 — Cytochrome f, large domain
Family Family familyb.2.6.1 — Cytochrome f, large domain
Domain ID domain_idd1q90a2
Class classb — All beta proteins
Fold Fold foldb.84 — Barrel-sandwich hybrid
Superfamily Superfamily superfamilyb.84.2 — Rudiment single hybrid motif
Family Family familyb.84.2.2 — Cytochrome f, small domain
Domain ID domain_idd1q90a3
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.23 — Single transmembrane helix
Superfamily Superfamily superfamilyf.23.23 — Cytochrome f subunit of the cytochrome b6f complex, transmembrane anchor
Family Family familyf.23.23.1 — Cytochrome f subunit of the cytochrome b6f complex, transmembrane anchor
Domain ID domain_idd1q90a4
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags
Domain ID domain_idd1q90b_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.21 — Heme-binding four-helical bundle
Superfamily Superfamily superfamilyf.21.1 — Transmembrane di-heme cytochromes
Family Family familyf.21.1.2 — Cytochrome b of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)
Domain ID domain_idd1q90c_
Class classb — All beta proteins
Fold Fold foldb.33 — ISP domain
Superfamily Superfamily superfamilyb.33.1 — ISP domain
Family Family familyb.33.1.1 — Rieske iron-sulfur protein (ISP)
Domain ID domain_idd1q90d_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.32 — a domain/subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)
Superfamily Superfamily superfamilyf.32.1 — a domain/subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)
Family Family familyf.32.1.1 — a domain/subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)
Domain ID domain_idd1q90g_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.23 — Single transmembrane helix
Superfamily Superfamily superfamilyf.23.26 — PetG subunit of the cytochrome b6f complex
Family Family familyf.23.26.1 — PetG subunit of the cytochrome b6f complex
Domain ID domain_idd1q90l_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.23 — Single transmembrane helix
Superfamily Superfamily superfamilyf.23.24 — PetL subunit of the cytochrome b6f complex
Family Family familyf.23.24.1 — PetL subunit of the cytochrome b6f complex
Domain ID domain_idd1q90m_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.23 — Single transmembrane helix
Superfamily Superfamily superfamilyf.23.25 — PetM subunit of the cytochrome b6f complex
Family Family familyf.23.25.1 — PetM subunit of the cytochrome b6f complex
Domain ID domain_idd1q90n_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.23 — Single transmembrane helix
Superfamily Superfamily superfamilyf.23.27 — PetN subunit of the cytochrome b6f complex
Family Family familyf.23.27.1 — PetN subunit of the cytochrome b6f complex
Domain ID domain_idd1q90r_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.23 — Single transmembrane helix
Superfamily Superfamily superfamilyf.23.12 — ISP transmembrane anchor
Family Family familyf.23.12.1 — ISP transmembrane anchor

CATH v4.4 (6 domains)

Domain ID domain_id1q90A01
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology40 — Immunoglobulin-like
Homologous superfamily homologous superfamily830 — Cytochrome f large domain
Domain ID domain_id1q90A02
Class class2 — Mainly Beta
Architecture architecture40 — Beta Barrel
Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Homologous superfamily homologous superfamily100 — RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain
Domain ID domain_id1q90B00
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology810 — Cytochrome Bc1 Complex; Chain C
Homologous superfamily homologous superfamily10 — Cytochrome Bc1 Complex; Chain C
Domain ID domain_id1q90C00
Class class2 — Mainly Beta
Architecture architecture102 — 3-layer Sandwich
Topology topology10 — Rieske Iron-sulfur Protein
Homologous superfamily homologous superfamily10 — Rieske [2Fe-2S] iron-sulphur domain
Domain ID domain_id1q90D01
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homologous superfamily homologous superfamily510 — Single helix bin
Domain ID domain_id1q90D02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology287 — Helix Hairpins
Homologous superfamily homologous superfamily980 — plastocyanin oxidoreductase
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8. Citations (2)

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9. Files and Curves (10)