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1L6E
Solution structure of the docking and dimerization domain of protein kinase A II-alpha (RIIalpha D/D). Alternatively called the N-terminal dimerization domain of the regulatory subunit of protein kinase A.
Deposited 2002-03-08
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Different construct
Different experimental conditions
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Assembly 1
Protein homooligomer
Homooligomer；Protein × 2
PDB declaration: dimeric
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Chain A
2–43（42 aa）
Fragment：N-terminal docking and dimerization domain
Chain B
2–43（42 aa）
Fragment：N-terminal docking and dimerization domain
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Not recorded
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No recorded non-water small molecule
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SOLUTION NMR
NMR measurement conditions
pH 4；298 K；Ionic strength (raw mmCIF value) 0.012；Pressure 1
NMR measurement conditions
pH 4；298 K；Ionic strength (raw mmCIF value) 0.012；Pressure 1
NMR measurement conditions
pH 4；298 K；Ionic strength (raw mmCIF value) 0.012；Pressure 1
NMR measurement conditions
pH 4；298 K；Ionic strength (raw mmCIF value) 0.012；Pressure 1
NMR sample composition
2mM dimer protein in 20 mM sodium phosphate buffer, 90%H2O-10% D2O, at pH 4 and temperature 298K | 90% H2O/10% D2O
NMR sample composition
1mM dimer protein 15N in 20 mM sodium phosphate buffer, 90%H2O-10% D2O, at pH 4 and temperature 298K | 90% H2O/10% D2O
NMR sample composition
1mM dimer protein 13C-15N in 20 mM sodium phosphate buffer, 90%H2O-10% D2O, at pH 4 and temperature 298K | 90% H2O/10% D2O
NMR sample composition
Mixture of 2mM unlabeled and 2 mM 13C-15N labeled sample unfolded in 5 M guanadine hydrochloride and refolded in 20 mM sodium phosphate buffer, 90% H2O-10% D2O, at pH 4 and temperature 298K | 90% H2O/10% D2O
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Resolution not provided
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2QVS
Crystal Structure of Type IIa Holoenzyme of cAMP-dependent Protein Kinase
Deposited 2007-08-08
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Different construct
Different mutation/modification
Different oligomeric state
Different experimental method
Different experimental conditions
Different structure-quality metrics
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Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
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Chain B
92–401（310 aa）
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Not recorded
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No recorded non-water small molecule
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X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；295.5 K；1.9 M sodium formate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.5K
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Resolution 2.50 Å
R-free 0.251
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9D2F
3D structure and atomic model of RS3 of mouse respiratory cilia
Deposited 2024-08-08
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Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
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Assembly 1
Protein heterocomplex
Heteromer；Protein × 29
PDB declaration: 29-meric
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Chain b
1–401（401 aa）
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Not recorded
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ZN ZINC ION × 2
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ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；30 mM HEPES pH 7.4, 25 mM KCl, 5 mM MgSO4, 1 mM EGTA, 0.1 mM EDTA
cryo-EM vitrification conditions
Cryogen ETHANE；4 microliters of splayed axoneme sample were applied to glow-discharged Quantifoil R2/2 Cu grids. After removing excess liquid by blotting the grid from the backside with Whatman filter paper for 2-3 s, the grid was immediately plunge-frozen in liquid ethane using a home-made plunge freezer.
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Resolution 4.70 Å
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