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1L6E
Solution structure of the docking and dimerization domain of protein kinase A II-alpha (RIIalpha D/D). Alternatively called the N-terminal dimerization domain of the regulatory subunit of protein kinase A.
提交 2002-03-08
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构建体不同
实验环境不同
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Assembly 1
蛋白同源多聚体
同源多聚体；蛋白 × 2
PDB 声明：dimeric
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链 A
2–43（42 aa）
片段：N-terminal docking and dimerization domain
链 B
2–43（42 aa）
片段：N-terminal docking and dimerization domain
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未记录
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未记录非水小分子
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SOLUTION NMR
NMR测量条件
pH 4；298 K；离子强度（mmCIF原始值）0.012；压力 1
NMR测量条件
pH 4；298 K；离子强度（mmCIF原始值）0.012；压力 1
NMR测量条件
pH 4；298 K；离子强度（mmCIF原始值）0.012；压力 1
NMR测量条件
pH 4；298 K；离子强度（mmCIF原始值）0.012；压力 1
NMR样品组成
2mM dimer protein in 20 mM sodium phosphate buffer, 90%H2O-10% D2O, at pH 4 and temperature 298K | 90% H2O/10% D2O
NMR样品组成
1mM dimer protein 15N in 20 mM sodium phosphate buffer, 90%H2O-10% D2O, at pH 4 and temperature 298K | 90% H2O/10% D2O
NMR样品组成
1mM dimer protein 13C-15N in 20 mM sodium phosphate buffer, 90%H2O-10% D2O, at pH 4 and temperature 298K | 90% H2O/10% D2O
NMR样品组成
Mixture of 2mM unlabeled and 2 mM 13C-15N labeled sample unfolded in 5 M guanadine hydrochloride and refolded in 20 mM sodium phosphate buffer, 90% H2O-10% D2O, at pH 4 and temperature 298K | 90% H2O/10% D2O
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分辨率未提供
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2QVS
Crystal Structure of Type IIa Holoenzyme of cAMP-dependent Protein Kinase
提交 2007-08-08
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构建体不同
突变/修饰不同
聚集状态不同
实验方法不同
实验环境不同
结构质量不同
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Assembly 1
蛋白异源复合物
异源复合物；蛋白 × 2
PDB 声明：dimeric
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链 B
92–401（310 aa）
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未记录
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未记录非水小分子
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X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 5.5；295.5 K；1.9 M sodium formate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.5K
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分辨率 2.50 Å
R-free 0.251
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9D2F
3D structure and atomic model of RS3 of mouse respiratory cilia
提交 2024-08-08
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构建体不同
突变/修饰不同
聚集状态不同
配体/离子不同
实验方法不同
实验环境不同
结构质量不同
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Assembly 1
蛋白异源复合物
异源复合物；蛋白 × 29
PDB 声明：29-meric
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链 b
1–401（401 aa）
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未记录
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ZN ZINC ION × 2
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ELECTRON MICROSCOPY
cryo-EM缓冲液
pH 7.4；30 mM HEPES pH 7.4, 25 mM KCl, 5 mM MgSO4, 1 mM EGTA, 0.1 mM EDTA
cryo-EM玻璃化条件
冷冻剂 ETHANE；4 microliters of splayed axoneme sample were applied to glow-discharged Quantifoil R2/2 Cu grids. After removing excess liquid by blotting the grid from the backside with Whatman filter paper for 2-3 s, the grid was immediately plunge-frozen in liquid ethane using a home-made plunge freezer.
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分辨率 4.70 Å
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