1rio

Structure of bacteriophage lambda cI-NTD in complex with sigma-region4 of Thermus aquaticus bound to DNA

Method: X-RAY DIFFRACTION Dmax: 89.5 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 1rio

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1rio
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1rio
沉积日期 deposition_date2003-11-17
结构标题 titleStructure of bacteriophage lambda cI-NTD in complex with sigma-region4 of Thermus aquaticus bound to DNA
关键词 keywordsHELIX-TURN-HELIX, TRANSCRIPTION ACTIVATION, transcription-DNA COMPLEX; transcription/DNA
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier26.11
回转半径 Rg (电子) rg_electron25.24
零角强度 I(0) i053072200.00
分子量 molecular_weight45486.0 kDa
排除体积 excluded_volume51967 ų
包络体积 envelope_volume69424 ų
水化壳体积 shell_volume24061 ų
包络直径 envelope_diameter92.5
壳层 Rg shell_rg31.24
包络 Rg envelope_rg25.25
形状 Rg shape_rg25.25
总 Rg total_rg25.75
总原子数 total_atoms3110
残基数 n_residues302
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax89.5
Rg (实空间) rg_real26.27
Rg 误差 (实空间) rg_real_error0.96
I(0) (实空间) i0_real5.3070e+07
I(0) 误差 (实空间) i0_real_error8.0580e+05
Rg (倒空间) rg_reciprocal26.22
I(0) (倒空间) i0_reciprocal53070000.0000
解质量估计 total_estimate0.8674
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary29.4
偏度 Skewness skewness0.495
峰度 Kurtosis kurtosis-0.194
角度范围 angular_range— – 0.3050 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha4791000.0000
实空间数据点数 n_real_points62
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.819; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.855; Smooth: 0.964

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (7)

6. 折叠分类 (SCOP + CATH) 8 domains

SCOP 2.08 (5 domains)

结构域编号 domain_idd1rioa1
类 Class classa — All alpha proteins
折叠类型 Fold folda.35 — lambda repressor-like DNA-binding domains
超家族 Superfamily superfamilya.35.1 — lambda repressor-like DNA-binding domains
家族 Family familya.35.1.2 — Phage repressors
结构域编号 domain_idd1rioa2
类 Class classl — Artifacts
折叠类型 Fold foldl.1 — Tags
超家族 Superfamily superfamilyl.1.1 — Tags
家族 Family familyl.1.1.1 — Tags
结构域编号 domain_idd1riob1
类 Class classa — All alpha proteins
折叠类型 Fold folda.35 — lambda repressor-like DNA-binding domains
超家族 Superfamily superfamilya.35.1 — lambda repressor-like DNA-binding domains
家族 Family familya.35.1.2 — Phage repressors
结构域编号 domain_idd1riob2
类 Class classl — Artifacts
折叠类型 Fold foldl.1 — Tags
超家族 Superfamily superfamilyl.1.1 — Tags
家族 Family familyl.1.1.1 — Tags
结构域编号 domain_idd1rioh_
类 Class classa — All alpha proteins
折叠类型 Fold folda.4 — DNA/RNA-binding 3-helical bundle
超家族 Superfamily superfamilya.4.13 — Sigma3 and sigma4 domains of RNA polymerase sigma factors
家族 Family familya.4.13.2 — Sigma4 domain

CATH v4.4 (3 domains)

结构域编号 domain_id1rioA00
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology260 — 434 Repressor (Amino-terminal Domain)
同源超家族 H-superfamily homologous superfamily40 — lambda repressor-like DNA-binding domains
结构域编号 domain_id1rioB00
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology260 — 434 Repressor (Amino-terminal Domain)
同源超家族 H-superfamily homologous superfamily40 — lambda repressor-like DNA-binding domains
结构域编号 domain_id1rioH00
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

7. 引用文献 (1)

8. 文件与曲线 (10)