Glutamate receptor, ionotropic kainate 2
Rattus norvegicus
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein homooligomer Homooligomer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count | Chain A； UniProt 429–544 Chain A； UniProt 667–806 Chain B； UniProt 429–544 Chain B； UniProt 667–806 | Fragment：residues 1-259 | CL CHLORIDE ION × 4 QUS (S)-2-AMINO-3-(3,5-DIOXO-[1,2,4]OXADIAZOLIDIN-2-YL)-PROPIONIC ACID × 4 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 4.6；293 K；14% PEG 3350, 50mM Malonate, 20mM NaCl, 1mM EDTA, 4.5mM Quisqualic acid, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K | Resolution 1.80 Å R-free 0.255 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 1S9T | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1S50 X-ray structure of the GluR6 ligand binding core (S1S2A) in complex with glutamate at 1.65 A resolution Deposited 2004-01-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
428–544（117 aa）
Fragment：GluR6 ligand binding core
Chain A
665–806（142 aa）
Fragment：GluR6 ligand binding core
|
Not recorded | GLU GLUTAMIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；24% PEG 4000 2 TRIS 20 NaCl 1 EDTA 10 NaGlu , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.65 Å R-free 0.230 |
| 1S7Y Crystal structure of the GluR6 ligand binding core in complex with glutamate at 1.75 A resolution orthorhombic form Deposited 2004-01-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–684（256 aa）
Fragment：GluR6 S1S2
Chain B
429–684（256 aa）
Fragment：GluR6 S1S2
|
Mutation：;1st Gly is vector encoded; remaining sequence corresponds to S398-K513 & P636-E755 of the mature GluR6 peptide joined by a vector encoded GT linker ; Mutation：;1st Gly is vector encoded; remaining sequence corresponds to S398-K513 & P636-E755 of the mature GluR6 peptide joined by a vector encoded GT linker ; | GLU GLUTAMIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；293 K；14% PEG 3350, 10 Citrate, 20 NaCl, 1 EDTA, 10 NaGlutamate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.75 Å R-free 0.243 |
| 1SD3 Crystal structure of the GLUR6 ligand binding core in complex with 2S,4R-4-methylglutamate at 1.8 Angstrom resolution Deposited 2004-02-12 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
429–544（116 aa）
Fragment：residues 1-259
Chain A
667–806（140 aa）
Fragment：residues 1-259
Chain B
429–544（116 aa）
Fragment：residues 1-259
Chain B
667–806（140 aa）
Fragment：residues 1-259
|
Not recorded | SYM 2S,4R-4-METHYLGLUTAMATE × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.9；293 K；14% PEG 3350, 10mM malonate, 10mM BME, 20mM NaCl, 2mM Citrate, 1mM EDTA, 4.5mM 2S,4R-4-methylglutamate, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.256 |
| 1TT1 CRYSTAL STRUCTURE OF THE GLUR6 LIGAND BINDING CORE IN COMPLEX WITH KAINATE 1.93 A RESOLUTION Deposited 2004-06-21 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Chain A
665–806（142 aa）
Chain B
429–544（116 aa）
Chain B
665–806（142 aa）
|
Not recorded | KAI 3-(CARBOXYMETHYL)-4-ISOPROPENYLPROLINE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
streak seeding；pH 4；297 K；18% PEG 3350, 25 mM Citrate, 2 mM Malonate, 20 mM NaCl, 1 mM EDTA, 10 mM BME, pH 4.0, streak seeding, temperature 297K
|
Resolution 1.93 Å R-free 0.216 |
| 2I0B Crystal structure of the GluR6 ligand binding core ELKQ mutant dimer at 1.96 Angstroms Resolution Deposited 2006-08-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
429–544（116 aa）
Chain A
667–806（140 aa）
|
Mutation：K494E, I749L, Q753K, E757Q Mutation：K494E, I749L, Q753K, E757Q | GLU GLUTAMIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；Reservoir: 1.5 M Ammonium Sulfate, 0.1 M NaCl, 0.1 M HEPES. 0.015 M Na Acetate;
Protein: 12-22 mg/ml, 10 mM L-glutamate, 20 mM NaCl, 1 mM EDTA, pH 7.0, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.96 Å R-free 0.211 |
| 2I0B Crystal structure of the GluR6 ligand binding core ELKQ mutant dimer at 1.96 Angstroms Resolution Deposited 2006-08-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
429–544（116 aa）
Chain B
667–806（140 aa）
Chain C
429–544（116 aa）
Chain C
667–806（140 aa）
|
Mutation：K494E, I749L, Q753K, E757Q Mutation：K494E, I749L, Q753K, E757Q Mutation：K494E, I749L, Q753K, E757Q Mutation：K494E, I749L, Q753K, E757Q | GLU GLUTAMIC ACID × 2 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；Reservoir: 1.5 M Ammonium Sulfate, 0.1 M NaCl, 0.1 M HEPES. 0.015 M Na Acetate;
Protein: 12-22 mg/ml, 10 mM L-glutamate, 20 mM NaCl, 1 mM EDTA, pH 7.0, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.96 Å R-free 0.211 |
| 2I0C Crystal structure of the GluR6 ligand binding core dimer crosslinked by disulfide bonds between Y490C and L752C at 2.25 Angstroms Resolution Deposited 2006-08-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Chain A
667–806（140 aa）
Chain B
429–544（116 aa）
Chain B
667–806（140 aa）
|
Mutation：Y490C, L752C, S775E Mutation：Y490C, L752C, S775E Mutation：Y490C, L752C, S775E Mutation：Y490C, L752C, S775E | GLU GLUTAMIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；Reservoir: 20% isopropanol, 25% PEG 4k, 0.1 M NaCitrate.
Protein: 7-15 mg/ml, 10 mM HEPES, 20 mM NaCl, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.25 Å R-free 0.244 |
| 2XXR Crystal structure of the GluK2 (GluR6) wild-type LBD dimer in complex with glutamate Deposited 2010-11-11 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain A
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
|
Not recorded | GLU GLUTAMIC ACID × 2 CL CHLORIDE ION × 1 NA SODIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP. 27% PEG 4000, 3% PROPAN-2-OL, 80MM SODIUM ACETATE
|
Resolution 1.60 Å R-free 0.195 |
| 2XXT Crystal structure of the GluK2 (GluR6) wild-type LBD dimer in complex with kainate Deposited 2010-11-12 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain A
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
|
Not recorded | KAI 3-(CARBOXYMETHYL)-4-ISOPROPENYLPROLINE × 2 NA SODIUM ION × 2 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP 23% PEG 4000, 9% PROPAN-2-OL, 80MM SODIUM ACETATE.
|
Resolution 1.90 Å R-free 0.209 |
| 2XXU Crystal structure of the GluK2 (GluR6) M770K LBD dimer in complex with glutamate Deposited 2010-11-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain A
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
|
Mutation：YES Mutation：YES Mutation：YES Mutation：YES | GLU GLUTAMIC ACID × 2 CL CHLORIDE ION × 1 NA SODIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP. 27% PEG 4000, 9% PROPAN-2-OL, 80MM SODIUM ACETATE
|
Resolution 1.50 Å R-free 0.189 |
| 2XXV Crystal structure of the GluK2 (GluR6) M770K LBD dimer in complex with kainate Deposited 2010-11-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain A
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
|
Mutation：YES Mutation：YES Mutation：YES Mutation：YES | KAI 3-(CARBOXYMETHYL)-4-ISOPROPENYLPROLINE × 2 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP 27% PEG 4000, 6% PROPAN-2-OL, 80MM SODIUM ACETATE
|
Resolution 1.70 Å R-free 0.203 |
| 2XXW Crystal structure of the GluK2 (GluR6) D776K LBD dimer in complex with glutamate Deposited 2010-11-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain A
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
|
Mutation：YES Mutation：YES Mutation：YES Mutation：YES | GLU GLUTAMIC ACID × 2 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP 21% PEG 4000, 80MM SODIUM ACETATE
|
Resolution 2.30 Å R-free 0.244 |
| 2XXX Crystal structure of the GluK2 (GluR6) D776K LBD dimer in complex with glutamate (P21 21 21) Deposited 2010-11-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain A
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
|
Mutation：YES Mutation：YES Mutation：YES Mutation：YES | GLU GLUTAMIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP 21% PEG 4000, 9% PROPAN-2-OL, 80MM SODIUM ACETATE
|
Resolution 2.10 Å R-free 0.231 |
| 2XXX Crystal structure of the GluK2 (GluR6) D776K LBD dimer in complex with glutamate (P21 21 21) Deposited 2010-11-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain C
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain C
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain D
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain D
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
|
Mutation：YES Mutation：YES Mutation：YES Mutation：YES | GLU GLUTAMIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP 21% PEG 4000, 9% PROPAN-2-OL, 80MM SODIUM ACETATE
|
Resolution 2.10 Å R-free 0.231 |
| 2XXY Crystal structure of the GluK2 (GluR6) D776K LBD dimer in complex with kainate Deposited 2010-11-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain A
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
|
Mutation：YES Mutation：YES Mutation：YES Mutation：YES | KAI 3-(CARBOXYMETHYL)-4-ISOPROPENYLPROLINE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP 21% PEG 4000, 3% PROPAN-2-OL, 80MM SODIUM ACETATE
|
Resolution 3.00 Å R-free 0.264 |
| 2XXY Crystal structure of the GluK2 (GluR6) D776K LBD dimer in complex with kainate Deposited 2010-11-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain C
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain C
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain D
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain D
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
|
Mutation：YES Mutation：YES Mutation：YES Mutation：YES | KAI 3-(CARBOXYMETHYL)-4-ISOPROPENYLPROLINE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP 21% PEG 4000, 3% PROPAN-2-OL, 80MM SODIUM ACETATE
|
Resolution 3.00 Å R-free 0.264 |
| 3G3F Crystal structure of the GluR6 ligand binding domain dimer with glutamate and NaCl at 1.38 Angstrom resolution Deposited 2009-02-02 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：residues 429-544, 667-806
Chain A
667–806（140 aa）
Fragment：residues 429-544, 667-806
Chain B
429–544（116 aa）
Fragment：residues 429-544, 667-806
Chain B
667–806（140 aa）
Fragment：residues 429-544, 667-806
|
Not recorded | GLU GLUTAMIC ACID × 2 CL CHLORIDE ION × 1 NA SODIUM ION × 4 IPA ISOPROPYL ALCOHOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；16% PEG4K, 12% isopropanol, 0.1M NaCitrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.38 Å R-free 0.175 |
| 3G3G Crystal structure of the GluR6 ligand binding domain dimer K665R mutant with glutamate and NaCl at 1.3 Angstrom resolution Deposited 2009-02-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：residues 429-544, 667-806
Chain A
667–806（140 aa）
Fragment：residues 429-544, 667-806
Chain B
429–544（116 aa）
Fragment：residues 429-544, 667-806
Chain B
667–806（140 aa）
Fragment：residues 429-544, 667-806
|
Mutation：K696R Mutation：K696R Mutation：K696R Mutation：K696R | GLU GLUTAMIC ACID × 2 CL CHLORIDE ION × 3 NA SODIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；15% PEG 4K, 13% ISOPROPANOL, 0.1 M NaCitrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.30 Å R-free 0.168 |
| 3G3H Crystal structure of the GluR6 ligand binding domain dimer K665R I749L Q753K mutant with glutamate and NaCl at 1.5 Angstrom resolution Deposited 2009-02-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：residues 429-544, 667-806
Chain A
667–806（140 aa）
Fragment：residues 429-544, 667-806
Chain B
429–544（116 aa）
Fragment：residues 429-544, 667-806
Chain B
667–806（140 aa）
Fragment：residues 429-544, 667-806
|
Mutation：K696R, I780L, Q784K Mutation：K696R, I780L, Q784K Mutation：K696R, I780L, Q784K Mutation：K696R, I780L, Q784K | GLU GLUTAMIC ACID × 2 CL CHLORIDE ION × 2 NA SODIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；15% PEG 4K, 13% ISOPROPANOL, 0.1 M NaCitrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.50 Å R-free 0.176 |
| 3G3I Crystal structure of the GluR6 ligand binding domain dimer I442H K494E I749L Q753K mutant with glutamate and NaCl at 1.37 Angstrom resolution Deposited 2009-02-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：residues 429-544, 667-806
Chain A
667–806（140 aa）
Fragment：residues 429-544, 667-806
Chain B
429–544（116 aa）
Fragment：residues 429-544, 667-806
Chain B
667–806（140 aa）
Fragment：residues 429-544, 667-806
|
Mutation：I473H, K525E, I780L, Q784K Mutation：I473H, K525E, I780L, Q784K Mutation：I473H, K525E, I780L, Q784K Mutation：I473H, K525E, I780L, Q784K | GLU GLUTAMIC ACID × 2 CL CHLORIDE ION × 1 NA SODIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；18% PEG 4K, 13% ISOPROPANOL, 0.1 M NaCitrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.37 Å R-free 0.176 |
| 3G3J Crystal structure of the GluR6 ligand binding domain dimer I442H K494E K665R I749L Q753K mutant with glutamate and NaCl at 1.32 Angstrom resolution Deposited 2009-02-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：residues 429-544, 667-806
Chain A
667–806（140 aa）
Fragment：residues 429-544, 667-806
Chain B
429–544（116 aa）
Fragment：residues 429-544, 667-806
Chain B
667–806（140 aa）
Fragment：residues 429-544, 667-806
|
Mutation：I473H, K525E, K696R, I780L, Q784K Mutation：I473H, K525E, K696R, I780L, Q784K Mutation：I473H, K525E, K696R, I780L, Q784K Mutation：I473H, K525E, K696R, I780L, Q784K | GLU GLUTAMIC ACID × 2 CL CHLORIDE ION × 2 NA SODIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；15% PEG 4K, 15% ISOPROPANOL, 0.1 M NaCitrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.32 Å R-free 0.179 |
| 3G3K Crystal structure of the GluR6 ligand binding domain dimer I442H K494E K665R I749L Q753K E757Q mutant with glutamate and NaCl at 1.24 Angstrom resolution Deposited 2009-02-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：residues 429-544, 667-806
Chain A
667–806（140 aa）
Fragment：residues 429-544, 667-806
Chain B
429–544（116 aa）
Fragment：residues 429-544, 667-806
Chain B
667–806（140 aa）
Fragment：residues 429-544, 667-806
|
Mutation：I473H, K525E, K696R, I780L, Q784K, E788Q Mutation：I473H, K525E, K696R, I780L, Q784K, E788Q Mutation：I473H, K525E, K696R, I780L, Q784K, E788Q Mutation：I473H, K525E, K696R, I780L, Q784K, E788Q | GLU GLUTAMIC ACID × 2 CL CHLORIDE ION × 5 NA SODIUM ION × 2 IPA ISOPROPYL ALCOHOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；17% PEG 4K, 12% ISOPROPANOL, 0.1M NaCitrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.24 Å R-free 0.165 |
| 3H6G Crystal structure of the GluR6 amino terminal domain dimer assembly Deposited 2009-04-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
32–420（389 aa）
Fragment：UNP residues 32-420
Chain B
32–420（389 aa）
Fragment：UNP residues 32-420
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 TLA L(+)-TARTARIC ACID × 2 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；600 mM NaKTartrate, 200 mM NaCl, 20 mM NaAcetate, 1 mM EDTA, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.70 Å R-free 0.227 |
| 3H6H Crystal structure of the GluR6 amino terminal domain dimer assembly MPD form Deposited 2009-04-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
32–420（389 aa）
Fragment：UNP residues 32-420
Chain B
32–420（389 aa）
Fragment：UNP residues 32-420
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；293 K；100 mM Bicine, 10% MPD, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.90 Å R-free 0.254 |
| 3QLT Crystal structure of a GluK2 (GluR6) glycan wedge homodimer assembly Deposited 2011-02-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
32–420（389 aa）
Chain B
32–420（389 aa）
|
Mutation：A213N,G215S Mutation：A213N,G215S | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；10% PEG 8K, 20% ethylene glycol, 0.02 M each 1,6-hexanediol, 1-butanol, (RS)-1,2-propanediol, 2-propanol, 1,4 butanediol, 1,3-propanediol, and 0.1 M MOPS/HEPES-Na, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.99 Å R-free 0.293 |
| 3QLU Crystal structure of the GluK2/GluK5 (GluR6/KA2) ATD dimer assembly Deposited 2011-02-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
32–420（389 aa）
|
Mutation：A213N,G215S | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.2；293 K；0.15 M Ammonium Sulfate; 0.1 M Tris; 18% PEG 4K, pH 8.20, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.91 Å R-free 0.256 |
| 3QLU Crystal structure of the GluK2/GluK5 (GluR6/KA2) ATD dimer assembly Deposited 2011-02-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
32–420（389 aa）
|
Mutation：A213N,G215S | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CL CHLORIDE ION × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.2；293 K；0.15 M Ammonium Sulfate; 0.1 M Tris; 18% PEG 4K, pH 8.20, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.91 Å R-free 0.256 |
| 3QLV Crystal structure of the GluK2/GluK5 (GluR6/KA2) ATD tetramer assembly Deposited 2011-02-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
32–420（389 aa）
Chain D
32–420（389 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；293 K；10% Ethylene Glycol, 0.1 M HEPES, 5% PEG 8K, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.94 Å R-free 0.280 |
| 3QLV Crystal structure of the GluK2/GluK5 (GluR6/KA2) ATD tetramer assembly Deposited 2011-02-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain F
32–420（389 aa）
Chain H
32–420（389 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；293 K；10% Ethylene Glycol, 0.1 M HEPES, 5% PEG 8K, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.94 Å R-free 0.280 |
| 3QLV Crystal structure of the GluK2/GluK5 (GluR6/KA2) ATD tetramer assembly Deposited 2011-02-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain J
32–420（389 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；293 K；10% Ethylene Glycol, 0.1 M HEPES, 5% PEG 8K, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.94 Å R-free 0.280 |
| 3QLV Crystal structure of the GluK2/GluK5 (GluR6/KA2) ATD tetramer assembly Deposited 2011-02-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
32–420（389 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；293 K；10% Ethylene Glycol, 0.1 M HEPES, 5% PEG 8K, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.94 Å R-free 0.280 |
| 3QLV Crystal structure of the GluK2/GluK5 (GluR6/KA2) ATD tetramer assembly Deposited 2011-02-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
32–420（389 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；293 K；10% Ethylene Glycol, 0.1 M HEPES, 5% PEG 8K, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.94 Å R-free 0.280 |
| 3QLV Crystal structure of the GluK2/GluK5 (GluR6/KA2) ATD tetramer assembly Deposited 2011-02-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain F
32–420（389 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；293 K；10% Ethylene Glycol, 0.1 M HEPES, 5% PEG 8K, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.94 Å R-free 0.280 |
| 3QLV Crystal structure of the GluK2/GluK5 (GluR6/KA2) ATD tetramer assembly Deposited 2011-02-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 7 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain H
32–420（389 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；293 K；10% Ethylene Glycol, 0.1 M HEPES, 5% PEG 8K, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.94 Å R-free 0.280 |
| 3QLV Crystal structure of the GluK2/GluK5 (GluR6/KA2) ATD tetramer assembly Deposited 2011-02-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 8 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain J
32–420（389 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；293 K；10% Ethylene Glycol, 0.1 M HEPES, 5% PEG 8K, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.94 Å R-free 0.280 |
| 4BDL Crystal structure of the GluK2 K531A LBD dimer in complex with glutamate Deposited 2012-10-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain A
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
|
Mutation：YES Mutation：YES Mutation：YES Mutation：YES | GLU GLUTAMIC ACID × 2 NA SODIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
19% PEG 4,000, 3% PROPAN-2-OL, 80MM NA ACETATE
|
Resolution 1.75 Å R-free 0.202 |
| 4BDM Crystal structure of the GluK2 K531A LBD dimer in complex with kainate Deposited 2012-10-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain A
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
|
Mutation：YES Mutation：YES Mutation：YES Mutation：YES | KAI 3-(CARBOXYMETHYL)-4-ISOPROPENYLPROLINE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
21% PEG 4,000, 9% PROPAN-2-OL, 80MM NA ACETATE
|
Resolution 3.40 Å R-free 0.289 |
| 4BDM Crystal structure of the GluK2 K531A LBD dimer in complex with kainate Deposited 2012-10-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain C
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain C
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain D
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain D
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
|
Mutation：YES Mutation：YES Mutation：YES Mutation：YES | KAI 3-(CARBOXYMETHYL)-4-ISOPROPENYLPROLINE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
21% PEG 4,000, 9% PROPAN-2-OL, 80MM NA ACETATE
|
Resolution 3.40 Å R-free 0.289 |
| 4BDN Crystal structure of the GluK2 K531A-T779G LBD dimer in complex with glutamate Deposited 2012-10-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain A
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
|
Mutation：YES Mutation：YES Mutation：YES Mutation：YES | GLU GLUTAMIC ACID × 2 NA SODIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
25% PEG 4,000, 6% PROPAN-2-OL, 80MM NA ACETATE
|
Resolution 2.50 Å R-free 0.248 |
| 4BDN Crystal structure of the GluK2 K531A-T779G LBD dimer in complex with glutamate Deposited 2012-10-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain C
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain C
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain D
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain D
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
|
Mutation：YES Mutation：YES Mutation：YES Mutation：YES | GLU GLUTAMIC ACID × 2 NA SODIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
25% PEG 4,000, 6% PROPAN-2-OL, 80MM NA ACETATE
|
Resolution 2.50 Å R-free 0.248 |
| 4BDO Crystal structure of the GluK2 K531A-T779G LBD dimer in complex with kainate Deposited 2012-10-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain A
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
|
Mutation：YES Mutation：YES Mutation：YES Mutation：YES | KAI 3-(CARBOXYMETHYL)-4-ISOPROPENYLPROLINE × 2 NA SODIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
27% PEG 4,000, 6% PROPAN-2-OL, 80MM NA ACETATE
|
Resolution 2.55 Å R-free 0.241 |
| 4BDO Crystal structure of the GluK2 K531A-T779G LBD dimer in complex with kainate Deposited 2012-10-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain C
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain C
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain D
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain D
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
|
Mutation：YES Mutation：YES Mutation：YES Mutation：YES | KAI 3-(CARBOXYMETHYL)-4-ISOPROPENYLPROLINE × 2 NA SODIUM ION × 2 GLU GLUTAMIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
27% PEG 4,000, 6% PROPAN-2-OL, 80MM NA ACETATE
|
Resolution 2.55 Å R-free 0.241 |
| 4BDQ Crystal structure of the GluK2 R775A LBD dimer in complex with glutamate Deposited 2012-10-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain A
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
|
Mutation：YES Mutation：YES Mutation：YES Mutation：YES | GLU GLUTAMIC ACID × 2 NA SODIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
21% PEG 4,000, 80MM NA ACETATE
|
Resolution 1.90 Å R-free 0.213 |
| 4BDR Crystal structure of the GluK2 R775A LBD dimer in complex with kainate Deposited 2012-10-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain A
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
429–544（116 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
Chain B
667–806（140 aa）
Fragment：LIGAND BINDING DOMAIN, RESIDUES 429-544,667-806
|
Mutation：YES Mutation：YES Mutation：YES Mutation：YES | KAI 3-(CARBOXYMETHYL)-4-ISOPROPENYLPROLINE × 2 CL CHLORIDE ION × 1 NA SODIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
23% PEG 4,000, 3% PROPAN-2-OL, 80MM NA ACETATE
|
Resolution 1.65 Å R-free 0.197 |
| 4H8I Structure of GluK2-LBD in complex with GluAzo Deposited 2012-09-22 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：SEE REMARK 999
Chain A
667–806（140 aa）
Fragment：SEE REMARK 999
Chain B
429–544（116 aa）
Fragment：SEE REMARK 999
Chain B
667–806（140 aa）
Fragment：SEE REMARK 999
|
Not recorded | 11W (4R)-4-[(2E)-3-{4-[(E)-phenyldiazenyl]phenyl}prop-2-en-1-yl]-L-glutamic acid × 1 CL CHLORIDE ION × 1 SO4 SULFATE ION × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；2.05 M lithium sulfate, 10 mM magnesium chloride, 50 mM MES sodium, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.204 |
| 4UQQ Electron density map of GluK2 desensitized state in complex with 2S,4R-4-methylglutamate Deposited 2014-06-24 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
32–908（877 aa）
Fragment：ATD LBD AND PARTIAL TMD, RESIDUES 32-908
Chain B
32–908（877 aa）
Fragment：ATD LBD AND PARTIAL TMD, RESIDUES 32-908
Chain C
32–908（877 aa）
Fragment：ATD LBD AND PARTIAL TMD, RESIDUES 32-908
Chain D
32–908（877 aa）
Fragment：ATD LBD AND PARTIAL TMD, RESIDUES 32-908
|
Mutation：YES Mutation：YES Mutation：YES Mutation：YES | GLU GLUTAMIC ACID × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
150 MM NACL, 20 MM TRIS, 1 MM 2S,4R-4- METHYLGLUTAMATE, 0.75 MM DDM；pH 8；150 MM NACL, 20 MM TRIS, 1 MM 2S,4R-4- METHYLGLUTAMATE, 0.75 MM DDM
cryo-EM vitrification conditions
Cryogen ETHANE；ETHANE
|
Resolution 7.60 Å |
| 5CMK Crystal structure of the GluK2EM LBD dimer assembly complex with glutamate and LY466195 Deposited 2015-07-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Fragment：UNP residues 429-544, 667-806
Chain A
667–806（140 aa）
Fragment：UNP residues 429-544, 667-806
Chain B
429–544（116 aa）
Fragment：UNP residues 429-544, 667-806
Chain B
667–806（140 aa）
Fragment：UNP residues 429-544, 667-806
|
Mutation：A487T A658S N690S F704L,A487T A658S N690S F704L Mutation：A487T A658S N690S F704L,A487T A658S N690S F704L Mutation：A487T A658S N690S F704L,A487T A658S N690S F704L Mutation：A487T A658S N690S F704L,A487T A658S N690S F704L | GLU GLUTAMIC ACID × 1 LI LITHIUM ION × 2 CL CHLORIDE ION × 2 LY5 (3S,4aR,6S,8aR)-6-{[(2S)-2-carboxy-4,4-difluoropyrrolidin-1-yl]methyl}decahydroisoquinoline-3-carboxylic acid × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；Reservoir: 2M Li2SO4 3% PEG 4K 0.1M MgSO4 0.1 M NaAcetate
Protein buffer: 100 mM NaCl, 5 mM LY466195, 1 mM EDTA, 10 mM HEPES pH 7 No added glutamate
|
Resolution 1.80 Å R-free 0.187 |
| 5CMM Crystal structure of the GluK2EM LBD dimer assembly complex with 2S,4R-4-methylglutamate Deposited 2015-07-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
429–544（116 aa）
Fragment：UNP P42260 residues 429-544, UNP Q13002 residues 667-806
|
Mutation：A487T A658S N690S F704L,A487T A658S N690S F704L | SYM 2S,4R-4-METHYLGLUTAMATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；291 K；Reservoir: 18% PEG 4K
Protein buffer: 20 mM NaCl, 5 mM 2S,4R-4-methylglutamate, 1 mM EDTA, 2 mM TRIS pH 8
|
Resolution 1.27 Å R-free 0.163 |
| 5KUF GluK2EM with 2S,4R-4-methylglutamate Deposited 2016-07-13 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
32–908（877 aa）
Chain B
32–908（877 aa）
Chain C
32–908（877 aa）
Chain D
32–908（877 aa）
|
Not recorded | SYM 2S,4R-4-METHYLGLUTAMATE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 5KUH GluK2EM with LY466195 Deposited 2016-07-13 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
32–544（513 aa）
Fragment：UNP residues 32-544,667-908
Chain A
667–908（242 aa）
Fragment：UNP residues 32-544,667-908
Chain B
32–544（513 aa）
Fragment：UNP residues 32-544,667-908
Chain B
667–908（242 aa）
Fragment：UNP residues 32-544,667-908
Chain C
32–544（513 aa）
Fragment：UNP residues 32-544,667-908
Chain C
667–908（242 aa）
Fragment：UNP residues 32-544,667-908
Chain D
32–544（513 aa）
Fragment：UNP residues 32-544,667-908
Chain D
667–908（242 aa）
Fragment：UNP residues 32-544,667-908
|
Mutation：T487A, S538A, S570N, L584F Mutation：T487A, S538A, S570N, L584F Mutation：T487A, S538A, S570N, L584F Mutation：T487A, S538A, S570N, L584F Mutation：T487A, S538A, S570N, L584F Mutation：T487A, S538A, S570N, L584F Mutation：T487A, S538A, S570N, L584F Mutation：T487A, S538A, S570N, L584F | LY5 (3S,4aR,6S,8aR)-6-{[(2S)-2-carboxy-4,4-difluoropyrrolidin-1-yl]methyl}decahydroisoquinoline-3-carboxylic acid × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 11.60 Å |
| 7F56 DNQX-bound GluK2-1xNeto2 complex, with asymmetric LBD Deposited 2021-06-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Mutation：F107L Mutation：F107L Mutation：F107L Mutation：F107L | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 16 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 7F57 Kainate-bound GluK2-1xNeto2 complex, at the desensitized state Deposited 2021-06-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Mutation：F107L Mutation：F107L Mutation：F107L Mutation：F107L | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 11 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 7F59 DNQX-bound GluK2-1xNeto2 complex Deposited 2021-06-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Mutation：F107L Mutation：F107L Mutation：F107L Mutation：F107L | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 7 PGT (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 7F5A DNQX-bound GluK2-2xNeto2 complex Deposited 2021-06-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Mutation：F107L Mutation：F107L Mutation：F107L Mutation：F107L | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 10 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.40 Å |
| 7F5B LBD-TMD focused reconstruction of DNQX-bound GluK2-1xNeto2 complex Deposited 2021-06-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Mutation：F107L Mutation：F107L Mutation：F107L Mutation：F107L | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 CA CALCIUM ION × 1 PGT (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 7KS0 GluK2/K5 with 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX) Deposited 2020-11-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain B
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.30 Å |
| 7KS3 GluK2/K5 with L-Glu Deposited 2020-11-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain B
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.80 Å |
| 8F0O cryo-EM structure of homomeric kainate receptor GluK2 in resting (apo) state Deposited 2022-11-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Mutation：I567V, C576V, C595S Mutation：I567V, C576V, C595S Mutation：I567V, C576V, C595S Mutation：I567V, C576V, C595S | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.99 Å |
| 8FWQ Structure of kainate receptor GluK2 in complex with the positive allosteric modulator BPAM344 Deposited 2023-01-23 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 14 2J9 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide × 4 NA SODIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.96 Å |
| 8FWR Structure of the amino-terminal domain of kainate receptor GluK2 in complex with the positive allosteric modulator BPAM344 Deposited 2023-01-23 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8FWS Structure of the ligand-binding and transmembrane domains of kainate receptor GluK2 in complex with the positive allosteric modulator BPAM344 Deposited 2023-01-23 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | 2J9 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 16 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 NA SODIUM ION × 6 CL CHLORIDE ION × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.23 Å |
| 8FWT Structure of the amino terminal domain of kainate receptor GluK2 in complex with the positive allosteric modulator BPAM344 and competitive antagonist DNQX Deposited 2023-01-23 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.09 Å |
| 8FWU Structure of the ligand-binding and transmembrane domains of kainate receptor GluK2 in complex with the positive allosteric modulator BPAM344 and competitive antagonist DNQX Deposited 2023-01-23 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | DNQ 6,7-DINITROQUINOXALINE-2,3-DIONE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 16 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 NA SODIUM ION × 8 CL CHLORIDE ION × 2 2J9 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.18 Å |
| 8FWV Structure of the amino-terminal domain of kainate receptor GluK2 in complex with the positive allosteric modulator BPAM344 and noncompetitive inhibitor perampanel Deposited 2023-01-23 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.03 Å |
| 8FWW Structure of the ligand-binding and transmembrane domains of kainate receptor GluK2 in complex with the positive allosteric modulator BPAM344 and noncompetitive inhibitor perampanel Deposited 2023-01-23 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | 2J9 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 16 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 6ZP 2-(6'-oxo-1'-phenyl[1',6'-dihydro[2,3'-bipyridine]]-5'-yl)benzonitrile × 2 NA SODIUM ION × 7 CL CHLORIDE ION × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8GC2 Domoate-bound GluK2 kainate receptor in partially-open conformation 1 Deposited 2023-03-01 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
34–908（875 aa）
Chain B
34–908（875 aa）
Chain C
34–908（875 aa）
Chain D
34–908（875 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 DOQ (2S,3S,4S)-2-CARBOXY-4-[(1Z,3E,5R)-5-CARBOXY-1-METHYL-1,3-HEXADIENYL]-3-PYRROLIDINEACETIC ACID × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；50 mM Tris/HCl 150 mM NaCl 1mM DDM pH 8.0
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 8GC3 Domote-bound GluK2 kainate receptors in partially-open conformation 2 Deposited 2023-03-01 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
34–908（875 aa）
Chain B
34–908（875 aa）
Chain C
34–908（875 aa）
Chain D
34–908（875 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 DOQ (2S,3S,4S)-2-CARBOXY-4-[(1Z,3E,5R)-5-CARBOXY-1-METHYL-1,3-HEXADIENYL]-3-PYRROLIDINEACETIC ACID × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；50 mM Tris/HCl 150 mM NaCl 1mM DDM pH 8.0
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8GC4 Domoate-bound GluK2 kainate receptor in partially-open conformation 3 Deposited 2023-03-01 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
34–908（875 aa）
Chain B
34–908（875 aa）
Chain C
34–908（875 aa）
Chain D
34–908（875 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 DOQ (2S,3S,4S)-2-CARBOXY-4-[(1Z,3E,5R)-5-CARBOXY-1-METHYL-1,3-HEXADIENYL]-3-PYRROLIDINEACETIC ACID × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；50 mM Tris/HCl 150 mM NaCl 1mM DDM pH 8.0
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.93 Å |
| 8GC5 Domoate-bound GluK2 kainate receptors in non-active conformation Deposited 2023-03-01 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
34–908（875 aa）
Chain B
34–908（875 aa）
Chain C
34–908（875 aa）
Chain D
34–908（875 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 DOQ (2S,3S,4S)-2-CARBOXY-4-[(1Z,3E,5R)-5-CARBOXY-1-METHYL-1,3-HEXADIENYL]-3-PYRROLIDINEACETIC ACID × 4 MAN alpha-D-mannopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；50 mM Tris/HCl 150 mM NaCl 1mM DDM pH 8.0
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.93 Å |
| 8R32 Crystal structure of the GluK2 ligand-binding domain in complex with L-glutamate and BPAM344 at 1.60 A resolution Deposited 2023-11-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
429–544（116 aa）
Chain A
667–805（139 aa）
|
Not recorded | GLU GLUTAMIC ACID × 2 2J9 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide × 2 CL CHLORIDE ION × 4 NA SODIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；25% PEG4000, 9% propan-2-ol, 0.1
mM sodium acetate, pH 5.5
|
Resolution 1.60 Å R-free 0.178 |
| 8R32 Crystal structure of the GluK2 ligand-binding domain in complex with L-glutamate and BPAM344 at 1.60 A resolution Deposited 2023-11-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
429–544（116 aa）
Chain B
667–805（139 aa）
|
Not recorded | GLU GLUTAMIC ACID × 2 2J9 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide × 2 CL CHLORIDE ION × 4 NA SODIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；25% PEG4000, 9% propan-2-ol, 0.1
mM sodium acetate, pH 5.5
|
Resolution 1.60 Å R-free 0.178 |
| 9B35 Ligand-binding and transmembrane domains of kainate receptor GluK2 in the open state, a complex with agonist glutamate and positive allosteric modulator BPAM344 Deposited 2024-03-18 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | GLU GLUTAMIC ACID × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9B36 Open state of kainate receptor GluK2 in complex with agonist glutamate and positive allosteric modulator BPAM344 bound to two concanavalin A dimers. Composite map. Deposited 2024-03-18 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | 2J9 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide × 4 GLU GLUTAMIC ACID × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 14 CLR CHOLESTEROL × 8 ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.29 Å |
| 9B37 Open state of kainate receptor GluK2 in complex with agonist glutamate and positive allosteric modulator BPAM344 bound to one concanavalin A dimer. Composite map. Deposited 2024-03-18 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | 2J9 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide × 4 GLU GLUTAMIC ACID × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 14 CLR CHOLESTEROL × 8 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ZN ZINC ION × 2 CA CALCIUM ION × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.66 Å |
| 9B38 Kainate receptor GluK2 in complex with agonist glutamate with pseudo 4-fold symmetrical ligand-binding domain layer Deposited 2024-03-18 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | GLU GLUTAMIC ACID × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 18 MAN alpha-D-mannopyranose × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.36 Å |
| 9B39 Kainate receptor GluK2 in complex with agonist glutamate with asymmetric ligand-binding domain layer Deposited 2024-03-18 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | GLU GLUTAMIC ACID × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 20 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.84 Å |
| 9C5Y Structure of the amino-terminal domain of kainate receptor GluK2 in the apo state Deposited 2024-06-07 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen NITROGEN
|
Resolution 3.10 Å |
| 9C5Z Structure of Ligand binding and transmembrane domains of kainate receptor Gluk2 in apo state Deposited 2024-06-07 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen NITROGEN
|
Resolution 3.63 Å |
| 9C60 CryoEM structure of kainate receptor Gluk2 in apo state Deposited 2024-06-07 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 12 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen NITROGEN
|
Resolution 3.80 Å |
| 9CAZ Structure of kainate receptor Gluk2 in apo state Deposited 2024-06-18 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 11 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen NITROGEN
|
Resolution 3.88 Å |
| 9DXQ Ligand-binding and transmembrane domains of kainate receptor GluK2 in complex with positive allosteric modulator BPAM-344 and channel blocker Philanthotoxin-74 Deposited 2024-10-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Mutation：I567V,Y571C Mutation：I567V,Y571C Mutation：I567V,Y571C Mutation：I567V,Y571C | 2J9 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 16 CLR CHOLESTEROL × 8 NA SODIUM ION × 6 CL CHLORIDE ION × 2 A1BDR N-{7-[(4-aminobutyl)amino]heptyl}-Nalpha-butanoyl-D-tyrosinamide × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.81 Å |
| 9DXR Ligand-binding and transmembrane domains of kainate receptor GluK2 in complex with positive allosteric modulator BPAM-344 and channel blocker Nephilatoxin-8 Deposited 2024-10-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Mutation：I567V,Y571C Mutation：I567V,Y571C Mutation：I567V,Y571C Mutation：I567V,Y571C | 2J9 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 16 A1BDS N~1~-{5-[(N-{4-[(3-aminopropyl)amino]butyl}-beta-alanyl)amino]pentyl}-N~2~-[(1H-indol-3-yl)acetyl]-D-aspartamide × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9DXS Ligand-binding and transmembrane domains of kainate receptor GluK2 in complex with positive allosteric modulator BPAM-344 and channel blocker Spermine Deposited 2024-10-11 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | 2J9 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 12 CLR CHOLESTEROL × 8 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 SPM SPERMINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.55 Å |
| 9DXT Ligand-binding and transmembrane domains of kainate receptor GluK2 in complex with positive allosteric modulator BPAM-344 and channel blocker Kukoamine-A Deposited 2024-10-11 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | 2J9 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 8 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 16 CLR CHOLESTEROL × 8 AH2 1-deoxy-alpha-D-mannopyranose × 4 A1BDT N,N'-[butane-1,4-diylbis(azanediylpropane-3,1-diyl)]bis[3-(3,4-dihydroxyphenyl)propanamide] × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.75 Å |
| 9MZI CryoEM structure of GluK2 bound to glutamate in the deep desensitized state 3 Deposited 2025-01-23 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 13 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.83 Å |
| 9MZJ CryoEM structure of GluK2 bound to glutamate in the deep desensitized state 2 Deposited 2025-01-23 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 12 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.74 Å |
| 9MZK CryoEM structure of GluK2 bound to glutamate in the deep desensitized state 1 Deposited 2025-01-23 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 12 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.81 Å |
| 9MZL CryoEM structure of GluK2 bound to glutamate in the transition state Deposited 2025-01-23 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 9 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.02 Å |
| 9MZM CryoEM structure of GluK2 bound to glutamate in the shallow desensitized state, consensus map Deposited 2025-01-23 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 12 GLU GLUTAMIC ACID × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.86 Å |
| 9MZN cryoEM structure of GluK2 ATD in the shallow desensitized state Deposited 2025-01-23 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9MZO cryoEM structure of GluK2 LBD-TMD bound to glutamate in the shallow desensitized state Deposited 2025-01-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Mutation：N802C, K676C Mutation：N802C, K676C Mutation：N802C, K676C Mutation：N802C, K676C | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 GLU GLUTAMIC ACID × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.86 Å |
| 9MZP CryoEM structure of GluK2 bound to glutamate in the shallow desensitized state, composite map Deposited 2025-01-23 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 12 GLU GLUTAMIC ACID × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 9MZR cryoEM structure of GluK2 LBD-TMD bound to BPAM344 and glutamate in the non-active state Deposited 2025-01-23 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Mutation：K676C, N802C Mutation：K676C, N802C Mutation：K676C, N802C Mutation：K676C, N802C | 2J9 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide × 4 GLU GLUTAMIC ACID × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.93 Å |
| 9MZS cryoEM structure of GluK2 bound to BPAM344 and glutamate in the non-active state, composite map Deposited 2025-01-23 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 GLU GLUTAMIC ACID × 4 2J9 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 9N4L Composite map for GluK2 in the apo state with asymmetric ligand binding domain Deposited 2025-02-03 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 16 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.24 Å |
| 9N4M Composite map for GluK2 in the apo state with 2-fold symmetrical ligand-binding domain Deposited 2025-02-03 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 16 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 14 NA SODIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.77 Å |
| 9N4N Composite map for GluK2 in the open state, in complex with the positive allosteric modulator BPAM344 and agonist kainate Deposited 2025-02-03 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | KAI 3-(CARBOXYMETHYL)-4-ISOPROPENYLPROLINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 16 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 18 2J9 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.32 Å |
| 9N4O Composite map for GluK2-0xNeto2 in the apo state with asymmetric ligand-binding domain Deposited 2025-02-03 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 15 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 16 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 9N4P Composite map for GluK2-1xNeto2 in the apo state Deposited 2025-02-03 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 15 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 14 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.58 Å |
| 9N4Q Composite map for GluK2-2xNeto2 in the apo state Deposited 2025-02-03 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 15 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 15 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.48 Å |
| 9N4R Composite map for GluK2-0xNeto2 in the open state, in complex with the positive allosteric modulator BPAM344 and agonist kainate Deposited 2025-02-03 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | KAI 3-(CARBOXYMETHYL)-4-ISOPROPENYLPROLINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 16 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 20 2J9 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.48 Å |
| 9N4S Composite map for GluK2-1xNeto2 in the open state, in complex with the positive allosteric modulator BPAM344 and agonist kainate Deposited 2025-02-03 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | KAI 3-(CARBOXYMETHYL)-4-ISOPROPENYLPROLINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 17 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 19 2J9 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.34 Å |
| 9N4T Composite map for GluK2-2xNeto2 in the open state, in complex with the positive allosteric modulator BPAM344 and agonist kainate Deposited 2025-02-03 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
1–908（908 aa）
Chain B
1–908（908 aa）
Chain C
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | 2J9 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide × 4 KAI 3-(CARBOXYMETHYL)-4-ISOPROPENYLPROLINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 18 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 20 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.22 Å |
| 9OSF The intact LBD state of GluK2/K5 with 5-iodowillardiine and kynurenic acid sodium salt Deposited 2025-05-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain B
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；20 mM Tris, 300 mM NaCl, 0.35 mM DDM, pH 8.0
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.83 Å |
| 9OSG The partially ruptured LBD state of GluK2/K5 with 5-iodowillardiine and kynurenic acid sodium salt Deposited 2025-05-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain B
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.83 Å |
| 9OSI The intact LBD state of GluK2/K5 with alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) Deposited 2025-05-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain B
1–908（908 aa）
Chain D
1–908（908 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；20 mM Tris, 300 mM NaCl, 0.35 mM DDM, pH 8.0
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.95 Å |
91 other PDB entries and 106 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | GRIK2_RAT |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 2–117； UniProt 429–544 Author chain A； PDBConstruct 120–259； UniProt 667–806 Author chain B； PDBConstruct 2–117； UniProt 429–544 Author chain B； PDBConstruct 120–259； UniProt 667–806 |
