1sqj

Crystal Structure Analysis of Oligoxyloglucan reducing-end-specific cellobiohydrolase (OXG-RCBH)

Method: X-RAY DIFFRACTION Dmax: 151.4 Å Quality: REASONABLE

SAXS 散射曲线 SAXS Profile

SAXS profile for 1sqj

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1sqj
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1sqj
沉积日期 deposition_date2004-03-19
结构标题 titleCrystal Structure Analysis of Oligoxyloglucan reducing-end-specific cellobiohydrolase (OXG-RCBH)
关键词 keywordsBETA-PROPELLER, HYDROLASE; HYDROLASE
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier47.48
回转半径 Rg (电子) rg_electron48.37
零角强度 I(0) i0419551000.00
分子量 molecular_weight165560.0 kDa
排除体积 excluded_volume205080 ų
包络体积 envelope_volume265830 ų
水化壳体积 shell_volume49283 ų
包络直径 envelope_diameter161.6
壳层 Rg shell_rg46.65
包络 Rg envelope_rg48.10
形状 Rg shape_rg48.37
总 Rg total_rg48.31
总原子数 total_atoms11709
残基数 n_residues1541
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax151.4
Rg (实空间) rg_real48.29
Rg 误差 (实空间) rg_real_error1.46
I(0) (实空间) i0_real4.1960e+08
I(0) 误差 (实空间) i0_real_error6.7930e+06
Rg (倒空间) rg_reciprocal47.49
I(0) (倒空间) i0_reciprocal419100000.0000
解质量估计 total_estimate0.7348
解质量评级 solution_quality REASONABLE a REASONABLE solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary36.7
偏度 Skewness skewness0.542
峰度 Kurtosis kurtosis-0.618
角度范围 angular_range— – 0.1650 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha32350000.0000
实空间数据点数 n_real_points34
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.632; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.644; Smooth: 0.008

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (2)

6. 折叠分类 (SCOP + CATH) 8 domains

SCOP 2.08 (4 domains)

结构域编号 domain_idd1sqja1
类 Class classb — All beta proteins
折叠类型 Fold foldb.69 — 7-bladed beta-propeller
超家族 Superfamily superfamilyb.69.13 — Oligoxyloglucan reducing end-specific cellobiohydrolase
家族 Family familyb.69.13.1 — Oligoxyloglucan reducing end-specific cellobiohydrolase
结构域编号 domain_idd1sqja2
类 Class classb — All beta proteins
折叠类型 Fold foldb.69 — 7-bladed beta-propeller
超家族 Superfamily superfamilyb.69.13 — Oligoxyloglucan reducing end-specific cellobiohydrolase
家族 Family familyb.69.13.1 — Oligoxyloglucan reducing end-specific cellobiohydrolase
结构域编号 domain_idd1sqjb1
类 Class classb — All beta proteins
折叠类型 Fold foldb.69 — 7-bladed beta-propeller
超家族 Superfamily superfamilyb.69.13 — Oligoxyloglucan reducing end-specific cellobiohydrolase
家族 Family familyb.69.13.1 — Oligoxyloglucan reducing end-specific cellobiohydrolase
结构域编号 domain_idd1sqjb2
类 Class classb — All beta proteins
折叠类型 Fold foldb.69 — 7-bladed beta-propeller
超家族 Superfamily superfamilyb.69.13 — Oligoxyloglucan reducing end-specific cellobiohydrolase
家族 Family familyb.69.13.1 — Oligoxyloglucan reducing end-specific cellobiohydrolase

CATH v4.4 (4 domains)

结构域编号 domain_id1sqjA01
类 Class class2 — Mainly Beta
架构 Architecture architecture130 — 7 Propeller
拓扑 Topology topology10 — Methylamine Dehydrogenase; Chain H
同源超家族 H-superfamily homologous superfamily10 — YVTN repeat-like/Quinoprotein amine dehydrogenase
结构域编号 domain_id1sqjA02
类 Class class2 — Mainly Beta
架构 Architecture architecture130 — 7 Propeller
拓扑 Topology topology10 — Methylamine Dehydrogenase; Chain H
同源超家族 H-superfamily homologous superfamily10 — YVTN repeat-like/Quinoprotein amine dehydrogenase
结构域编号 domain_id1sqjB01
类 Class class2 — Mainly Beta
架构 Architecture architecture130 — 7 Propeller
拓扑 Topology topology10 — Methylamine Dehydrogenase; Chain H
同源超家族 H-superfamily homologous superfamily10 — YVTN repeat-like/Quinoprotein amine dehydrogenase
结构域编号 domain_id1sqjB02
类 Class class2 — Mainly Beta
架构 Architecture architecture130 — 7 Propeller
拓扑 Topology topology10 — Methylamine Dehydrogenase; Chain H
同源超家族 H-superfamily homologous superfamily10 — YVTN repeat-like/Quinoprotein amine dehydrogenase

7. 引用文献 (3)

8. 文件与曲线 (10)