1tbg

BETA-GAMMA DIMER OF THE HETEROTRIMERIC G-PROTEIN TRANSDUCIN

Method: X-RAY DIFFRACTION D_max: 124.9 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	1tbg
沉积日期 deposition_date	1996-06-15
结构标题 title	BETA-GAMMA DIMER OF THE HETEROTRIMERIC G-PROTEIN TRANSDUCIN
关键词 keywords	COMPLEX (GTP-BINDING-TRANSDUCER), EYE, TRANSDUCER, PRENYLATION, COMPLEX (GTP-BINDING-TRANSDUCER) complex; COMPLEX (GTP-BINDING/TRANSDUCER)
实验方法 method	X-RAY DIFFRACTION

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	39.06 Å
回转半径 Rg (电子) rg_electron	38.76 Å
零角强度 I(0) i0	533529000.00
分子量 molecular_weight	179260.0 kDa
排除体积 excluded_volume	220310 Å³
包络体积 envelope_volume	283090 Å³
水化壳体积 shell_volume	60177 Å³
包络直径 envelope_diameter	132.1 Å
壳层 Rg shell_rg	45.20 Å
包络 Rg envelope_rg	38.37 Å
形状 Rg shape_rg	38.78 Å
总 Rg total_rg	39.03 Å
总原子数 total_atoms	12541
残基数 n_residues	1623
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	124.9 Å
Rg (实空间) rg_real	38.92 Å
Rg 误差 (实空间) rg_real_error	0.75 Å
I(0) (实空间) i0_real	5.3350e+08
I(0) 误差 (实空间) i0_real_error	8.3290e+06
Rg (倒空间) rg_reciprocal	39.01 Å
I(0) (倒空间) i0_reciprocal	533600000.0000
解质量估计 total_estimate	0.9016
解质量评级 solution_quality	EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	46.8 Å
偏度 Skewness skewness	0.184
峰度 Kurtosis kurtosis	-0.591
角度范围 angular_range	— – 0.2000 Å⁻¹
当前正则化参数 α current_alpha	0.0001
最高正则化参数 α highest_alpha	139200000.0000
实空间数据点数 n_real_points	41
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.933; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.919

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (3)

Entity 1 polymer

描述 description	TRANSDUCIN
分子量 formula_weight	37416.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	4

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D
原子数 atom_count	13273

MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Entity 2 polymer

描述 description	TRANSDUCIN
分子量 formula_weight	7980.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	4

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E,F,G,H

APVINIEDLTEKDKLKMEVDQLKKEVTLERMLVSKCCEEFRDYVEERSGEDPLVKGIPEDKNPFKELK

Entity 3 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	732

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6. 折叠分类 (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

结构域编号 domain_id	d1tbga_
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.69 — 7-bladed beta-propeller
超家族 Superfamily superfamily	b.69.4 — WD40 repeat-like
家族 Family family	b.69.4.1 — WD40-repeat

结构域编号 domain_id	d1tbgb_
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.69 — 7-bladed beta-propeller
超家族 Superfamily superfamily	b.69.4 — WD40 repeat-like
家族 Family family	b.69.4.1 — WD40-repeat

结构域编号 domain_id	d1tbgc_
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.69 — 7-bladed beta-propeller
超家族 Superfamily superfamily	b.69.4 — WD40 repeat-like
家族 Family family	b.69.4.1 — WD40-repeat

结构域编号 domain_id	d1tbgd_
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.69 — 7-bladed beta-propeller
超家族 Superfamily superfamily	b.69.4 — WD40 repeat-like
家族 Family family	b.69.4.1 — WD40-repeat

结构域编号 domain_id	d1tbge_
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.137 — Non-globular all-alpha subunits of globular proteins
超家族 Superfamily superfamily	a.137.3 — Transducin (heterotrimeric G protein), gamma chain
家族 Family family	a.137.3.1 — Transducin (heterotrimeric G protein), gamma chain

结构域编号 domain_id	d1tbgf_
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.137 — Non-globular all-alpha subunits of globular proteins
超家族 Superfamily superfamily	a.137.3 — Transducin (heterotrimeric G protein), gamma chain
家族 Family family	a.137.3.1 — Transducin (heterotrimeric G protein), gamma chain

结构域编号 domain_id	d1tbgg_
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.137 — Non-globular all-alpha subunits of globular proteins
超家族 Superfamily superfamily	a.137.3 — Transducin (heterotrimeric G protein), gamma chain
家族 Family family	a.137.3.1 — Transducin (heterotrimeric G protein), gamma chain

结构域编号 domain_id	d1tbgh_
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.137 — Non-globular all-alpha subunits of globular proteins
超家族 Superfamily superfamily	a.137.3 — Transducin (heterotrimeric G protein), gamma chain
家族 Family family	a.137.3.1 — Transducin (heterotrimeric G protein), gamma chain

CATH v4.4 (8 domains)

结构域编号 domain_id	1tbgA00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	130 — 7 Propeller
拓扑 Topology topology	10 — Methylamine Dehydrogenase; Chain H
同源超家族 H-superfamily homologous superfamily	10 — YVTN repeat-like/Quinoprotein amine dehydrogenase

结构域编号 domain_id	1tbgB00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	130 — 7 Propeller
拓扑 Topology topology	10 — Methylamine Dehydrogenase; Chain H
同源超家族 H-superfamily homologous superfamily	10 — YVTN repeat-like/Quinoprotein amine dehydrogenase

结构域编号 domain_id	1tbgC00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	130 — 7 Propeller
拓扑 Topology topology	10 — Methylamine Dehydrogenase; Chain H
同源超家族 H-superfamily homologous superfamily	10 — YVTN repeat-like/Quinoprotein amine dehydrogenase

结构域编号 domain_id	1tbgD00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	130 — 7 Propeller
拓扑 Topology topology	10 — Methylamine Dehydrogenase; Chain H
同源超家族 H-superfamily homologous superfamily	10 — YVTN repeat-like/Quinoprotein amine dehydrogenase

结构域编号 domain_id	1tbgE00
类 Class class	4 — Few Secondary Structures
架构 Architecture architecture	10 — Irregular
拓扑 Topology topology	260 — G Protein Gi Gamma 2
同源超家族 H-superfamily homologous superfamily	10 — Transducin (heterotrimeric G protein), gamma chain

结构域编号 domain_id	1tbgF00
类 Class class	4 — Few Secondary Structures
架构 Architecture architecture	10 — Irregular
拓扑 Topology topology	260 — G Protein Gi Gamma 2
同源超家族 H-superfamily homologous superfamily	10 — Transducin (heterotrimeric G protein), gamma chain

结构域编号 domain_id	1tbgG00
类 Class class	4 — Few Secondary Structures
架构 Architecture architecture	10 — Irregular
拓扑 Topology topology	260 — G Protein Gi Gamma 2
同源超家族 H-superfamily homologous superfamily	10 — Transducin (heterotrimeric G protein), gamma chain

结构域编号 domain_id	1tbgH00
类 Class class	4 — Few Secondary Structures
架构 Architecture architecture	10 — Irregular
拓扑 Topology topology	260 — G Protein Gi Gamma 2
同源超家族 H-superfamily homologous superfamily	10 — Transducin (heterotrimeric G protein), gamma chain

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7. 引用文献 (5)

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晶胞边长 a length_a	85.100 Å
晶胞边长 b length_b	94.000 Å
晶胞边长 c length_c	194.700 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	16

空间群 space_group	P 21 21 21
空间群编号 int_tables_number	19
晶系 crystal_system	—

分辨率 resolution	2.100 Å
最低分辨率 resolution_low	6.000 Å
R 因子 r_factor	0.2000
R_work r_work	0.2000
R_free r_free	0.3060

fit	/app/data/saxs_computation/tb/1tbg/1tbg.fit
pddf	/app/data/pr_computation/tb/1tbg/1tbg.pr.out
profile	/app/data/saxs_computation/tb/1tbg/1tbg.dat

BETA-GAMMA DIMER OF THE HETEROTRIMERIC G-PROTEIN TRANSDUCIN

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

5. 实体与聚合体信息 (3)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 water

6. 折叠分类 (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

CATH v4.4 (8 domains)

7. 引用文献 (5)

8. 文件与曲线 (10)

Files (7)

Curves (3)