SAXSdb — 1tq9

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Ribonuclease, seminal

OrganismNot specified

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein homooligomer Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count	Chain A； UniProt 27–150 Chain B； UniProt 27–150	Non-standard monomer：Yes (specific site not provided by mmCIF)	CPA 2'-DEOXYCYTIDINE-2'-DEOXYADENOSINE-3',5'-MONOPHOSPHATE × 2	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；PEG 8000, isopropanol, sodium acetate, cacodilate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K	Resolution 2.00 Å R-free 0.269

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 1TQ9	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
11BA BINDING OF A SUBSTRATE ANALOGUE TO A DOMAIN SWAPPING PROTEIN IN THE COMPLEX OF BOVINE SEMINAL RIBONUCLEASE WITH URIDYLYL-2',5'-ADENOSINE Deposited 1999-03-17	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain not uniquely mapped Reference range not declared Chain A 27–150（124 aa）	Not recorded	SO4 SULFATE ION × 3 UPA URIDYLYL-2'-5'-PHOSPHO-ADENOSINE × 2	X-RAY DIFFRACTION X-ray crystallization conditions pH 4.8；pH 4.8	Resolution 2.06 Å
1BSR BOVINE SEMINAL RIBONUCLEASE STRUCTURE AT 1.9 ANGSTROMS RESOLUTION Deposited 1993-04-28	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 27–150（124 aa） Chain B 27–150（124 aa）	Not recorded	SO4 SULFATE ION × 7	X-RAY DIFFRACTION mmCIF provides none of the parsed conditions	Resolution 1.90 Å
1BSR BOVINE SEMINAL RIBONUCLEASE STRUCTURE AT 1.9 ANGSTROMS RESOLUTION Deposited 1993-04-28	Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 27–150（124 aa） Chain B 27–150（124 aa）	Not recorded	SO4 SULFATE ION × 14	X-RAY DIFFRACTION mmCIF provides none of the parsed conditions	Resolution 1.90 Å
1N1X Crystal Structure Analysis of the monomeric [S-carboxyamidomethyl-Cys31, S-carboxyamidomethyl-Cys32] Bovine seminal ribonuclease Deposited 2002-10-21	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 27–150（124 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.7；293 K；PEG 4000, TRIS-HCl, sodium acetate, acetonitrile, glycerol (cryoprotectant), pH 8.7, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K	Resolution 1.45 Å R-free 0.258
1N3Z Crystal structure of the [S-carboxyamidomethyl-Cys31, S-carboxyamidomethyl-Cys32] monomeric derivative of the bovine seminal ribonuclease in the liganded state Deposited 2002-10-30	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 27–150（124 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	U3P 3'-URIDINEMONOPHOSPHATE × 1 ADN ADENOSINE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.7；293 K；PEG 4000, sodium acetate, acetonitrile, glycerol (cryoprotectant), TRIS-HCl, pH 8.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 1.65 Å R-free 0.230
1QWQ Solution structure of the monomeric N67D mutant of Bovine Seminal Ribonuclease Deposited 2003-09-03	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 27–150（124 aa）	Mutation：N67D	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 5.65；300 K；Ionic strength (raw mmCIF value) 0；Pressure ambient NMR sample composition 2.0MM MONOMERIC BOVINE SEMINAL RIBONUCLEASE U-97% 15N \| 95% H2O/5% D2O NMR sample composition 2.0MM MONOMERIC BOVINE SEMINAL RIBONUCLEASE \| 95% H2O/5% D2O	Resolution not provided
1R3M Crystal structure of the dimeric unswapped form of bovine seminal ribonuclease Deposited 2003-10-02	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 27–150（124 aa）	Not recorded	PO4 PHOSPHATE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 8.4；294 K；MPD, ammonium sulfate, pH 8.4, EVAPORATION, temperature 294K	Resolution 2.20 Å R-free 0.292
1R3M Crystal structure of the dimeric unswapped form of bovine seminal ribonuclease Deposited 2003-10-02	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 27–150（124 aa）	Not recorded	PO4 PHOSPHATE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 8.4；294 K；MPD, ammonium sulfate, pH 8.4, EVAPORATION, temperature 294K	Resolution 2.20 Å R-free 0.292
1R5C X-ray structure of the complex of Bovine seminal ribonuclease swapping dimer with d(CpA) Deposited 2003-10-10	Different mutation/modification Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 27–150（124 aa） Chain B 27–150（124 aa）	Not recorded	CPA 2'-DEOXYCYTIDINE-2'-DEOXYADENOSINE-3',5'-MONOPHOSPHATE × 2	X-RAY DIFFRACTION X-ray crystallization conditions MICROBATCH；pH 5.4；293 K；62-64% saturation ammonium sulfate, 8-10% 2-methyl-2,4-pentandiol, dinucleotide-protein molar ratio of 8:1, pH 5.4, MICRO-BATCH, temperature 293K	Resolution 2.10 Å R-free 0.242
1R5D X-ray structure of bovine seminal ribonuclease swapping dimer from a new crystal form Deposited 2003-10-10	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 27–150（124 aa） Chain B 27–150（124 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；PEG 4000, 0.1 M sodium acetate, 0.1 M TRIS-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 2.50 Å R-free 0.255
1Y92 Crystal structure of the P19A/N67D Variant Of Bovine seminal Ribonuclease Deposited 2004-12-14	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 27–150（124 aa） Chain B 27–150（124 aa）	Mutation：P19A, N67D Mutation：P19A, N67D	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；PEG 4000, Tris-HCl, sodium acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 2.20 Å R-free 0.202
1Y94 Crystal structure of the G16S/N17T/P19A/S20A/N67D Variant Of Bovine seminal Ribonuclease Deposited 2004-12-14	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 27–150（124 aa） Chain B 27–150（124 aa）	Mutation：G16S, N17T, P19A, S20A, N67D Mutation：G16S, N17T, P19A, S20A, N67D	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；PEG 4000, TRIS-HCL, SODIUM ACETATE, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 2.20 Å R-free 0.259
2LFJ Solution structure of the monomeric derivative of BS-RNase Deposited 2011-07-06	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 27–150（124 aa）	Mutation：N67D	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 5.8；300 K NMR sample composition 1 mM [U-100% 13C; U-100% 15N] mBS, 90% H2O/10% D2O \| 90% H2O/10% D2O NMR sample composition 1 mM [U-100% 15N] mBS, 90% H2O/10% D2O \| 90% H2O/10% D2O	Resolution not provided
3BCM Crystal Structure of The Unswapped Form of P19A/L28Q/N67D BS-RNase Deposited 2007-11-13	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 27–150（124 aa） Chain B 27–150（124 aa）	Mutation：P19A, L28Q, N67D Mutation：P19A, L28Q, N67D	PO4 PHOSPHATE ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.4；277 K；30% methyl pentanediol, 50mM TRIS-HCl pH 8.4, 0.1M ammonium phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 277K	Resolution 2.25 Å R-free 0.252
3BCM Crystal Structure of The Unswapped Form of P19A/L28Q/N67D BS-RNase Deposited 2007-11-13	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 27–150（124 aa） Chain B 27–150（124 aa）	Mutation：P19A, L28Q, N67D Mutation：P19A, L28Q, N67D	PO4 PHOSPHATE ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.4；277 K；30% methyl pentanediol, 50mM TRIS-HCl pH 8.4, 0.1M ammonium phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 277K	Resolution 2.25 Å R-free 0.252
3BCO Crystal Structure of The Swapped FOrm of P19A/L28Q/N67D BS-RNase Deposited 2007-11-13	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 27–150（124 aa） Chain B 27–150（124 aa）	Mutation：P19A, L28Q, N67D Mutation：P19A, L28Q, N67D	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；28% w/v PEG 8000, 0.2M SODIUM ACETATE, 0.1M CACODILATE pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	Resolution 2.25 Å R-free 0.250
3BCP Crystal Structure of The Swapped non covalent form of P19A/L28Q/N67D BS-RNase Deposited 2007-11-13	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 27–150（124 aa） Chain B 27–150（124 aa）	Mutation：P19A, L28Q, N67D Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：P19A, L28Q, N67D Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；27% w/v PEG 8000, 0.1M sodium phosphate, 0.2M calcium chloride, 0.1M cacodilate pH 5.3-5.9, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	Resolution 2.57 Å R-free 0.323
3BCP Crystal Structure of The Swapped non covalent form of P19A/L28Q/N67D BS-RNase Deposited 2007-11-13	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain C 27–150（124 aa） Chain D 27–150（124 aa）	Mutation：P19A, L28Q, N67D Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：P19A, L28Q, N67D Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；27% w/v PEG 8000, 0.1M sodium phosphate, 0.2M calcium chloride, 0.1M cacodilate pH 5.3-5.9, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	Resolution 2.57 Å R-free 0.323
3BCP Crystal Structure of The Swapped non covalent form of P19A/L28Q/N67D BS-RNase Deposited 2007-11-13	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 27–150（124 aa） Chain B 27–150（124 aa） Chain C 27–150（124 aa） Chain D 27–150（124 aa）	Mutation：P19A, L28Q, N67D Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：P19A, L28Q, N67D Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：P19A, L28Q, N67D Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：P19A, L28Q, N67D Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；27% w/v PEG 8000, 0.1M sodium phosphate, 0.2M calcium chloride, 0.1M cacodilate pH 5.3-5.9, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	Resolution 2.57 Å R-free 0.323
3DJO Bovine Seminal Ribonuclease uridine 2' phosphate complex Deposited 2008-06-24	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 27–150（124 aa） Chain B 27–150（124 aa）	Not recorded	U2P PHOSPHORIC ACID MONO-[2-(2,4-DIOXO-3,4-DIHYDRO-2H-PYRIMIDIN-1-YL)-4-HYDROXY-5-HYDROXYMETHYL-TETRAHYDRO-FURAN-3-YL] ESTER × 2 ACT ACETATE ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；289 K；30% PEG4000, 0.1M sodium acetate, 0.1M Tris/HCl, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 1.60 Å R-free 0.226
3DJP Bovine Seminal Ribonuclease- Uridine 3' phosphate complex Deposited 2008-06-24	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 27–150（124 aa） Chain B 27–150（124 aa）	Not recorded	UA3 URACIL ARABINOSE-3'-PHOSPHATE × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；289 K；30% PEG4000, 0.1M sodium acetate, 0.1M Tris/HCl, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 1.60 Å R-free 0.230
3DJQ Bovine Seminal Ribonuclease- Uridine 5' diphosphate complex Deposited 2008-06-24	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 27–150（124 aa） Chain B 27–150（124 aa）	Not recorded	UDP URIDINE-5'-DIPHOSPHATE × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；289 K；30% PEG4000, 0.1M sodium acetate, 0.1 M Tris/HCl, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 1.53 Å R-free 0.233
3DJV Bovine Seminal Ribonuclease- cytidine 3' phosphate complex Deposited 2008-06-24	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 27–150（124 aa） Chain B 27–150（124 aa）	Not recorded	C3P CYTIDINE-3'-MONOPHOSPHATE × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；289 K；30% PEG4000, 0.1M sodium acetate, 0.1M Tris/HCl, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 1.60 Å R-free 0.204
3DJX Bovine Seminal Ribonuclease- cytidine 5' phosphate complex Deposited 2008-06-24	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 27–150（124 aa） Chain B 27–150（124 aa）	Not recorded	C5P CYTIDINE-5'-MONOPHOSPHATE × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；289 K；30% PEG4000, 0.1M sodium acetate, 0.1M Tris/HCl, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 1.69 Å R-free 0.204
4N4C Crystal structure of the C-terminal swapped dimer of a Bovine seminal ribonuclease mutant Deposited 2013-10-08	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 27–150（124 aa） Fragment：UNP residues 27-150 Chain B 27–150（124 aa） Fragment：UNP residues 27-150	Mutation：G16S, N17T, P19A, S20A Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：G16S, N17T, P19A, S20A Non-standard monomer：Yes (specific site not provided by mmCIF)	PO4 PHOSPHATE ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION；pH 6；293 K；16-20% w/v PEG35K, 0.2 M lithium chloride, 0.1 M sodium cacodylate buffer, pH 6.0, 3% v/v acetonitrile, VAPOR DIFFUSION, temperature 293K	Resolution 2.48 Å R-free 0.186

20 other PDB entries and 25 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RNS_BOVIN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–124； UniProt 27–150 Author chain B； PDBConstruct 1–124； UniProt 27–150

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	1tq9
Deposition date deposition_date	2004-06-17
Structure title title	Non-covalent swapped dimer of Bovine Seminal Ribonuclease in complex with 2'-DEOXYCYTIDINE-2'-DEOXYADENOSINE-3',5'-MONOPHOSPHATE
Keywords keywords	Protein-dinucleotide complex, Cytotoxic action, Hydrolase; HYDROLASE
Experimental Method method	X-RAY DIFFRACTION

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	21.48 Å
Radius of gyration Rg (electron density) rg_electron	21.44 Å
Forward intensity I(0) i0	17295200.00
Molecular weight molecular_weight	28552.0 kDa
Excluded volume excluded_volume	34560 Å³
Envelope volume envelope_volume	41469 Å³
Hydration-shell volume shell_volume	17347 Å³
Envelope diameter envelope_diameter	79.1 Å
Shell Rg shell_rg	26.83 Å
Envelope Rg envelope_rg	21.80 Å
Shape Rg shape_rg	21.44 Å
Total Rg total_rg	22.12 Å
Total atoms total_atoms	1972
Residues n_residues	244
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	77.3 Å
Rg (real space) rg_real	21.70 Å
Rg uncertainty (real space) rg_real_error	0.69 Å
I(0) (real space) i0_real	1.7300e+07
I(0) uncertainty (real space) i0_real_error	2.4820e+05
Rg (reciprocal space) rg_reciprocal	21.66 Å
I(0) (reciprocal space) i0_reciprocal	17290000.0000
Solution quality estimate total_estimate	0.8095
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	2
Primary peak position r_peak_primary	20.5 Å
Skewness Skewness skewness	0.596
Kurtosis Kurtosis kurtosis	-0.094
Angular range angular_range	— – 0.3700 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	3555000.0000
Real-space data points n_real_points	69
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.658; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.625; Smooth: 0.921

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (3)

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7. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

Domain ID domain_id	d1tq9a_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.5 — RNase A-like
Superfamily Superfamily superfamily	d.5.1 — RNase A-like
Family Family family	d.5.1.1 — Ribonuclease A-like

Domain ID domain_id	d1tq9b_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.5 — RNase A-like
Superfamily Superfamily superfamily	d.5.1 — RNase A-like
Family Family family	d.5.1.1 — Ribonuclease A-like

CATH v4.4 (2 domains)

Domain ID domain_id	1tq9A00
Class class	3 — Alpha Beta
Architecture architecture	10 — Roll
Topology topology	130 — P-30 Protein
Homologous superfamily homologous superfamily	10 — Ribonuclease A-like domain

Domain ID domain_id	1tq9B00
Class class	3 — Alpha Beta
Architecture architecture	10 — Roll
Topology topology	130 — P-30 Protein
Homologous superfamily homologous superfamily	10 — Ribonuclease A-like domain

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8. Citations (8)

;Structure and Stability of the Non-covalent Swapped Dimer of Bovine Seminal Ribonuclease: AN ENZYME TAILORED TO EVADE RIBONUCLEASE PROTEIN INHIBITOR ;

J.Biol.Chem. (2004)

PMID: 15192098 · DOI: 10.1074/jbc.M405655200 PRIMARY

Population shift vs induced fit: the case of bovine seminal ribonuclease swapping dimer

Biopolymers (2004)

PMID: 15048772 · DOI: 10.1002/bip.20016

The unswapped chain of bovine seminal ribonuclease: Crystal structure of the free and liganded monomeric derivative

Proteins (2003)

PMID: 12833549 · DOI: 10.1002/prot.10407

Crystal structure of the dimeric unswapped form of bovine seminal ribonuclease

FEBS Lett. (2003)

PMID: 14596923 · DOI: 10.1016/S0014-5793(03)01114-1

A potential allosteric subsite generated by domain swapping in bovine seminal ribonuclease

J.Mol.Biol. (1999)

PMID: 10543951 · DOI: 10.1006/jmbi.1999.3158

;Binding of a substrate analog to a domain swapping protein: X-ray structure of the complex of bovine seminal ribonuclease with uridylyl(2',5')adenosine ;

Protein Sci. (1998)

PMID: 10082366

Swapping structural determinants of ribonucleases: an energetic analysis of the hinge peptide 16-22

Proc.Natl.Acad.Sci.USA (1995)

PMID: 7731986 · DOI: 10.1073/pnas.92.9.3799

Bovine seminal ribonuclease: structure at 1.9 A resolution

Acta Crystallogr.,Sect.D (1993)

DOI: 10.1107/S0907444993003403

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Unit-cell length a length_a	41.471 Å
Unit-cell length b length_b	69.689 Å
Unit-cell length c length_c	110.804 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	8

Space group space_group	P 21 21 21
Space-group number int_tables_number	19
Crystal system crystal_system	—

Resolution resolution	2.000 Å
Low-resolution limit resolution_low	10.000 Å
R factor r_factor	0.2120
R_work r_work	0.2060
R_free r_free	0.2690

Description description	Ribonuclease, seminal
Molecular weight formula_weight	13746.7 kDa
EC 编号 ec_number	3.1.27.5
Source method src_method	nat
Copy count entity_count	2

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B
Atom count atom_count	2093

Non-covalent swapped dimer of Bovine Seminal Ribonuclease in complex with 2'-DEOXYCYTIDINE-2'-DEOXYADENOSINE-3',5'-MONOPHOSPHATE

1. Protein Identity and Related Structures Protein Identity & Related Structures

Ribonuclease, seminal

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

6. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 water

7. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

CATH v4.4 (2 domains)

8. Citations (8)

9. Files and Curves (10)

Files (7)

Curves (3)

Description description	2'-DEOXYCYTIDINE-2'-DEOXYADENOSINE-3',5'-MONOPHOSPHATE
Molecular weight formula_weight	540.4 kDa
Source method src_method	syn
Copy count entity_count	2

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	121

fit	/app/data/saxs_computation/tq/1tq9/1tq9.fit
pddf	/app/data/pr_computation/tq/1tq9/1tq9.pr.out
profile	/app/data/saxs_computation/tq/1tq9/1tq9.dat