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1qwq

Solution structure of the monomeric N67D mutant of Bovine Seminal Ribonuclease

Method: SOLUTION NMR Dmax: 47.4 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Ribonuclease

Bos taurus

UniProt P00669

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 27–150 Mutation：N67D No other associated polymer SOLUTION NMR NMR measurement conditions：pH 5.65；300 K；Ionic strength (raw mmCIF value) 0；Pressure ambient NMR sample composition：2.0MM MONOMERIC BOVINE SEMINAL RIBONUCLEASE U-97% 15N | 95% H2O/5% D2O NMR sample composition：2.0MM MONOMERIC BOVINE SEMINAL RIBONUCLEASE | 95% H2O/5% D2O Resolution not provided

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 1QWQ Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11BA BINDING OF A SUBSTRATE ANALOGUE TO A DOMAIN SWAPPING PROTEIN IN THE COMPLEX OF BOVINE SEMINAL RIBONUCLEASE WITH URIDYLYL-2',5'-ADENOSINE Deposited 1999-03-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain not uniquely mapped Reference range not declared
Chain A 27–150（124 aa）
Not recorded SO4 SULFATE ION × 3 UPA URIDYLYL-2'-5'-PHOSPHO-ADENOSINE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.8；pH 4.8
Resolution 2.06 Å
1BSR BOVINE SEMINAL RIBONUCLEASE STRUCTURE AT 1.9 ANGSTROMS RESOLUTION Deposited 1993-04-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 27–150（124 aa）
Chain B 27–150（124 aa）
Not recorded SO4 SULFATE ION × 7 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å
1BSR BOVINE SEMINAL RIBONUCLEASE STRUCTURE AT 1.9 ANGSTROMS RESOLUTION Deposited 1993-04-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 27–150（124 aa）
Chain B 27–150（124 aa）
Not recorded SO4 SULFATE ION × 14 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å
1N1X Crystal Structure Analysis of the monomeric [S-carboxyamidomethyl-Cys31, S-carboxyamidomethyl-Cys32] Bovine seminal ribonuclease Deposited 2002-10-21 Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–150（124 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.7；293 K；PEG 4000, TRIS-HCl, sodium acetate, acetonitrile, glycerol (cryoprotectant), pH 8.7, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K
Resolution 1.45 Å R-free 0.258
1N3Z Crystal structure of the [S-carboxyamidomethyl-Cys31, S-carboxyamidomethyl-Cys32] monomeric derivative of the bovine seminal ribonuclease in the liganded state Deposited 2002-10-30 Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–150（124 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) U3P 3'-URIDINEMONOPHOSPHATE × 1 ADN ADENOSINE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.7；293 K；PEG 4000, sodium acetate, acetonitrile, glycerol (cryoprotectant), TRIS-HCl, pH 8.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.65 Å R-free 0.230
1R3M Crystal structure of the dimeric unswapped form of bovine seminal ribonuclease Deposited 2003-10-02 Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–150（124 aa）
Not recorded PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 8.4；294 K；MPD, ammonium sulfate, pH 8.4, EVAPORATION, temperature 294K
Resolution 2.20 Å R-free 0.292
1R3M Crystal structure of the dimeric unswapped form of bovine seminal ribonuclease Deposited 2003-10-02 Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 27–150（124 aa）
Not recorded PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 8.4；294 K；MPD, ammonium sulfate, pH 8.4, EVAPORATION, temperature 294K
Resolution 2.20 Å R-free 0.292
1R5C X-ray structure of the complex of Bovine seminal ribonuclease swapping dimer with d(CpA) Deposited 2003-10-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 27–150（124 aa）
Chain B 27–150（124 aa）
Not recorded CPA 2'-DEOXYCYTIDINE-2'-DEOXYADENOSINE-3',5'-MONOPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 5.4；293 K；62-64% saturation ammonium sulfate, 8-10% 2-methyl-2,4-pentandiol, dinucleotide-protein molar ratio of 8:1, pH 5.4, MICRO-BATCH, temperature 293K
Resolution 2.10 Å R-free 0.242
1R5D X-ray structure of bovine seminal ribonuclease swapping dimer from a new crystal form Deposited 2003-10-10 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 27–150（124 aa）
Chain B 27–150（124 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；PEG 4000, 0.1 M sodium acetate, 0.1 M TRIS-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.50 Å R-free 0.255
1TQ9 Non-covalent swapped dimer of Bovine Seminal Ribonuclease in complex with 2'-DEOXYCYTIDINE-2'-DEOXYADENOSINE-3',5'-MONOPHOSPHATE Deposited 2004-06-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 27–150（124 aa）
Chain B 27–150（124 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) CPA 2'-DEOXYCYTIDINE-2'-DEOXYADENOSINE-3',5'-MONOPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；PEG 8000, isopropanol, sodium acetate, cacodilate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 2.00 Å R-free 0.269
1Y92 Crystal structure of the P19A/N67D Variant Of Bovine seminal Ribonuclease Deposited 2004-12-14 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 27–150（124 aa）
Chain B 27–150（124 aa）
Mutation：P19A, N67D Mutation：P19A, N67D No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；PEG 4000, Tris-HCl, sodium acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.20 Å R-free 0.202
1Y94 Crystal structure of the G16S/N17T/P19A/S20A/N67D Variant Of Bovine seminal Ribonuclease Deposited 2004-12-14 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 27–150（124 aa）
Chain B 27–150（124 aa）
Mutation：G16S, N17T, P19A, S20A, N67D Mutation：G16S, N17T, P19A, S20A, N67D No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；PEG 4000, TRIS-HCL, SODIUM ACETATE, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.20 Å R-free 0.259
2LFJ Solution structure of the monomeric derivative of BS-RNase Deposited 2011-07-06 Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–150（124 aa）
Mutation：N67D No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 5.8；300 K
NMR sample composition 1 mM [U-100% 13C; U-100% 15N] mBS, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-100% 15N] mBS, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
3BCM Crystal Structure of The Unswapped Form of P19A/L28Q/N67D BS-RNase Deposited 2007-11-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 27–150（124 aa）
Chain B 27–150（124 aa）
Mutation：P19A, L28Q, N67D Mutation：P19A, L28Q, N67D PO4 PHOSPHATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.4；277 K；30% methyl pentanediol, 50mM TRIS-HCl pH 8.4, 0.1M ammonium phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 2.25 Å R-free 0.252
3BCM Crystal Structure of The Unswapped Form of P19A/L28Q/N67D BS-RNase Deposited 2007-11-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 27–150（124 aa）
Chain B 27–150（124 aa）
Mutation：P19A, L28Q, N67D Mutation：P19A, L28Q, N67D PO4 PHOSPHATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.4；277 K；30% methyl pentanediol, 50mM TRIS-HCl pH 8.4, 0.1M ammonium phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 2.25 Å R-free 0.252
3BCO Crystal Structure of The Swapped FOrm of P19A/L28Q/N67D BS-RNase Deposited 2007-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 27–150（124 aa）
Chain B 27–150（124 aa）
Mutation：P19A, L28Q, N67D Mutation：P19A, L28Q, N67D No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；28% w/v PEG 8000, 0.2M SODIUM ACETATE, 0.1M CACODILATE pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.25 Å R-free 0.250
3BCP Crystal Structure of The Swapped non covalent form of P19A/L28Q/N67D BS-RNase Deposited 2007-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 27–150（124 aa）
Chain B 27–150（124 aa）
Mutation：P19A, L28Q, N67D Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：P19A, L28Q, N67D Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；27% w/v PEG 8000, 0.1M sodium phosphate, 0.2M calcium chloride, 0.1M cacodilate pH 5.3-5.9, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.57 Å R-free 0.323
3BCP Crystal Structure of The Swapped non covalent form of P19A/L28Q/N67D BS-RNase Deposited 2007-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 27–150（124 aa）
Chain D 27–150（124 aa）
Mutation：P19A, L28Q, N67D Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：P19A, L28Q, N67D Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；27% w/v PEG 8000, 0.1M sodium phosphate, 0.2M calcium chloride, 0.1M cacodilate pH 5.3-5.9, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.57 Å R-free 0.323
3BCP Crystal Structure of The Swapped non covalent form of P19A/L28Q/N67D BS-RNase Deposited 2007-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 27–150（124 aa）
Chain B 27–150（124 aa）
Chain C 27–150（124 aa）
Chain D 27–150（124 aa）
Mutation：P19A, L28Q, N67D Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：P19A, L28Q, N67D Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：P19A, L28Q, N67D Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：P19A, L28Q, N67D Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；27% w/v PEG 8000, 0.1M sodium phosphate, 0.2M calcium chloride, 0.1M cacodilate pH 5.3-5.9, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.57 Å R-free 0.323
3DJO Bovine Seminal Ribonuclease uridine 2' phosphate complex Deposited 2008-06-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 27–150（124 aa）
Chain B 27–150（124 aa）
Not recorded U2P PHOSPHORIC ACID MONO-[2-(2,4-DIOXO-3,4-DIHYDRO-2H-PYRIMIDIN-1-YL)-4-HYDROXY-5-HYDROXYMETHYL-TETRAHYDRO-FURAN-3-YL] ESTER × 2 ACT ACETATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；289 K；30% PEG4000, 0.1M sodium acetate, 0.1M Tris/HCl, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 1.60 Å R-free 0.226
3DJP Bovine Seminal Ribonuclease- Uridine 3' phosphate complex Deposited 2008-06-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 27–150（124 aa）
Chain B 27–150（124 aa）
Not recorded UA3 URACIL ARABINOSE-3'-PHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；289 K；30% PEG4000, 0.1M sodium acetate, 0.1M Tris/HCl, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 1.60 Å R-free 0.230
3DJQ Bovine Seminal Ribonuclease- Uridine 5' diphosphate complex Deposited 2008-06-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 27–150（124 aa）
Chain B 27–150（124 aa）
Not recorded UDP URIDINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；289 K；30% PEG4000, 0.1M sodium acetate, 0.1 M Tris/HCl, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 1.53 Å R-free 0.233
3DJV Bovine Seminal Ribonuclease- cytidine 3' phosphate complex Deposited 2008-06-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 27–150（124 aa）
Chain B 27–150（124 aa）
Not recorded C3P CYTIDINE-3'-MONOPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；289 K；30% PEG4000, 0.1M sodium acetate, 0.1M Tris/HCl, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 1.60 Å R-free 0.204
3DJX Bovine Seminal Ribonuclease- cytidine 5' phosphate complex Deposited 2008-06-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 27–150（124 aa）
Chain B 27–150（124 aa）
Not recorded C5P CYTIDINE-5'-MONOPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；289 K；30% PEG4000, 0.1M sodium acetate, 0.1M Tris/HCl, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 1.69 Å R-free 0.204
4N4C Crystal structure of the C-terminal swapped dimer of a Bovine seminal ribonuclease mutant Deposited 2013-10-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 27–150（124 aa） Fragment：UNP residues 27-150
Chain B 27–150（124 aa） Fragment：UNP residues 27-150
Mutation：G16S, N17T, P19A, S20A Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：G16S, N17T, P19A, S20A Non-standard monomer：Yes (specific site not provided by mmCIF) PO4 PHOSPHATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6；293 K；16-20% w/v PEG35K, 0.2 M lithium chloride, 0.1 M sodium cacodylate buffer, pH 6.0, 3% v/v acetonitrile, VAPOR DIFFUSION, temperature 293K
Resolution 2.48 Å R-free 0.186

20 other PDB entries and 25 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RNS_BOVIN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–124； UniProt 27–150

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 1qwq

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 1qwq
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2. Structure Basics 2. Structure Basics

Entry ID entry_id1qwq
Deposition date deposition_date2003-09-03
Structure title titleSolution structure of the monomeric N67D mutant of Bovine Seminal Ribonuclease
Keywords keywordsALPHA-BETA-PROTEIN, Hydrolase; HYDROLASE
Experimental Method methodSOLUTION NMR
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier14.84 Å
Radius of gyration Rg (electron density) rg_electron14.93 Å
Forward intensity I(0) i0783615000.00
Molecular weight molecular_weight217910.0 kDa
Excluded volume excluded_volume265550 ų
Envelope volume envelope_volume32061 ų
Hydration-shell volume shell_volume16084 ų
Envelope diameter envelope_diameter54.9 Å
Shell Rg shell_rg22.77 Å
Envelope Rg envelope_rg17.03 Å
Shape Rg shape_rg14.89 Å
Total Rg total_rg15.21 Å
Total atoms total_atoms29904
Residues n_residues1984
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax47.4 Å
Rg (real space) rg_real14.80 Å
Rg uncertainty (real space) rg_real_error0.35 Å
I(0) (real space) i0_real7.8360e+08
I(0) uncertainty (real space) i0_real_error1.0330e+07
Rg (reciprocal space) rg_reciprocal14.81 Å
I(0) (reciprocal space) i0_reciprocal783600000.0000
Solution quality estimate total_estimate0.8206
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary15.6 Å
Skewness Skewness skewness0.208
Kurtosis Kurtosis kurtosis-0.586
Angular range angular_range— – 0.5000 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha541000.0000
Real-space data points n_real_points80
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.893; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.988; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (1)

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7. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

Domain ID domain_idd1qwqa_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.5 — RNase A-like
Superfamily Superfamily superfamilyd.5.1 — RNase A-like
Family Family familyd.5.1.1 — Ribonuclease A-like

CATH v4.4 (1 domains)

Domain ID domain_id1qwqA00
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology130 — P-30 Protein
Homologous superfamily homologous superfamily10 — Ribonuclease A-like domain
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8. Citations (2)

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9. Files and Curves (10)