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1u0d

Y33H Mutant of Homing endonuclease I-CreI

Method: X-RAY DIFFRACTION Dmax: 88.9 Å Quality: REASONABLE
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1. Protein Identity and Related Structures Protein Identity & Related Structures

DNA endonuclease I-CreI

Chlamydomonas reinhardtii

UniProt P05725

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Homooligomer Protein × 2 DNA 2 PDB declaration: tetrameric（4） Consistent with all polymer counts Chain A； UniProt 1–163 Chain B； UniProt 1–163 Not recorded 5'-D(*GP*CP*GP*AP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*CP*CP*G)-3' × 1 5'-D(*CP*GP*GP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*TP*TP*CP*GP*C)-3' × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 2.90 Å R-free 0.257

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 1U0D Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1AF5 GROUP I MOBILE INTRON ENDONUCLEASE Deposited 1997-03-21 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 4–138（135 aa）
Mutation：D56G No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 5；PROTEIN WAS CRYSTALLIZED FROM 23% PEG 6000, 0.1M CITRATE,PH 5.0
Resolution 3.00 Å R-free 0.373
1BP7 GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA Deposited 1998-08-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 2–153（152 aa）
Chain B 2–153（152 aa）
Not recorded CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；pH 6.5
Resolution 3.00 Å R-free 0.281
1BP7 GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA Deposited 1998-08-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain C 2–153（152 aa）
Chain D 2–153（152 aa）
Not recorded CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；pH 6.5
Resolution 3.00 Å R-free 0.281
1G9Y HOMING ENDONUCLEASE I-CREI / DNA SUBSTRATE COMPLEX WITH CALCIUM Deposited 2000-11-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 2–153（152 aa）
Chain B 2–153（152 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；25% PEG 400, pH 6.5, VAPOR DIFFUSION, HANGING DROP
Resolution 2.05 Å R-free 0.260
1G9Z LAGLIDADG HOMING ENDONUCLEASE I-CREI / DNA PRODUCT COMPLEX WITH MAGNESIUM Deposited 2000-11-28 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 2–153（152 aa）
Chain B 2–153（152 aa）
Not recorded MG MAGNESIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；30% PEG400, PH 6.5, VAPOR DIFFUSION, HANGING DROP
Resolution 1.80 Å R-free 0.249
1N3E Crystal structure of I-CreI bound to a palindromic DNA sequence I (palindrome of left side of wildtype DNA target sequence) Deposited 2002-10-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 1–163（163 aa）
Chain B 1–163（163 aa）
Not recorded CA CALCIUM ION × 3 NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；25% PEG 400, 20 mM NaCl, 10 mM CaCl2, 100 mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.50 Å R-free 0.245
1N3E Crystal structure of I-CreI bound to a palindromic DNA sequence I (palindrome of left side of wildtype DNA target sequence) Deposited 2002-10-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain G 1–163（163 aa）
Chain H 1–163（163 aa）
Not recorded CA CALCIUM ION × 3 NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；25% PEG 400, 20 mM NaCl, 10 mM CaCl2, 100 mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.50 Å R-free 0.245
1N3F Crystal structure of I-CreI bound to a palindromic DNA sequence II (palindrome of right side of wildtype DNA target sequence) Deposited 2002-10-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 1–163（163 aa）
Chain B 1–163（163 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；25% PEG 400, 20 mM NaCl, 10 mM CaCl2, 100 mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.00 Å R-free 0.228
1N3F Crystal structure of I-CreI bound to a palindromic DNA sequence II (palindrome of right side of wildtype DNA target sequence) Deposited 2002-10-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain G 1–163（163 aa）
Chain H 1–163（163 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；25% PEG 400, 20 mM NaCl, 10 mM CaCl2, 100 mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.00 Å R-free 0.228
1T9I I-CreI(D20N)/DNA complex Deposited 2004-05-17 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 1–163（163 aa）
Chain B 1–163（163 aa）
Mutation：D20N Mutation：D20N CA CALCIUM ION × 2 NA SODIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；PEG400, sodium chloride, calcium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP
Resolution 1.60 Å R-free 0.211
1T9J I-CreI(Q47E)/DNA complex Deposited 2004-05-17 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 1–163（163 aa）
Chain B 1–163（163 aa）
Mutation：Q47E Mutation：Q47E No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；PEG 400, sodium chloride, calcium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP
Resolution 2.00 Å R-free 0.268
1U0C Y33C Mutant of Homing endonuclease I-CreI Deposited 2004-07-13 Different ligand/ion Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 1–163（163 aa）
Chain B 1–163（163 aa）
Not recorded MG MAGNESIUM ION × 3 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.50 Å R-free 0.277
2I3P K28R mutant of Homing Endonuclease I-CreI Deposited 2006-08-20 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 1–153（153 aa）
Chain B 1–153（153 aa）
Mutation：K28R Mutation：K28R CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.5；298 K；PEG 400 28%, pH 6.5, VAPOR DIFFUSION, temperature 298K
Resolution 2.30 Å R-free 0.292
2I3Q Q44V mutant of Homing Endonuclease I-CreI Deposited 2006-08-20 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 1–153（153 aa）
Chain B 1–153（153 aa）
Mutation：Q44V Mutation：Q44V CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.5；298 K；PEG 400 28%, pH 6.5, VAPOR DIFFUSION, temperature 298K
Resolution 2.30 Å R-free 0.264
2O7M The C-terminal loop of the homing endonuclease I-CreI is essential for DNA binding and cleavage. Identification of a novel site for specificity engineering in the I-CreI scaffold Deposited 2006-12-11 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–156（156 aa） Fragment：residues 1-156
Chain B 1–156（156 aa） Fragment：residues 1-156
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.00 Å R-free 0.235
2VBJ Molecular basis of human XPC gene recognition and cleavage by engineered homing endonuclease heterodimers Deposited 2007-09-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 2–153（152 aa） Fragment：RESIDUES 2-153
Chain B 2–153（152 aa） Fragment：RESIDUES 2-153
Not recorded CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；HANGING-DROP DNA-PROTEIN COMPLEX SOLUTION WAS 4 MG/ML. 35% 2-ETHOXYETHANOL IN 0.1M NA-CACODYLATE PH6.5
Resolution 1.95 Å R-free 0.216
2VBL Molecular basis of human XPC gene recognition and cleavage by engineered homing endonuclease heterodimers Deposited 2007-09-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 1–153（153 aa） Fragment：RESIDUES 1-153
Chain B 1–153（153 aa） Fragment：RESIDUES 1-153
Not recorded MG MAGNESIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；4 MG/ML PROTEIN, 35% METHANOL, 0.1M NACACODYLATE PH 6.5
Resolution 1.80 Å R-free 0.197
2VBN Molecular basis of human XPC gene recognition and cleavage by engineered homing endonuclease heterodimers Deposited 2007-09-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 1–153（153 aa） Fragment：RESIDUES 1-153
Chain B 1–153（153 aa） Fragment：RESIDUES 1-153
Not recorded MG MAGNESIUM ION × 3 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；4 MG/ML PROTEIN, 20% PEG1000, 0.1M IMIDAZOLE PH 8.0, 0.2 M CAAC2
Resolution 1.90 Å R-free 0.223
2VBO Molecular basis of human XPC gene recognition and cleavage by engineered homing endonuclease heterodimers Deposited 2007-09-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 1–153（153 aa） Fragment：RESIDUES 1-153
Chain B 1–153（153 aa） Fragment：RESIDUES 1-153
Not recorded CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；pH 4.5
Resolution 1.80 Å R-free 0.225
4AAB Crystal structure of the mutant D75N I-CreI in complex with its wild- type target (The four central bases, 2NN region, are composed by GTAC from 5' to 3') Deposited 2011-12-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 2–153（152 aa）
Chain B 2–153（152 aa）
Mutation：YES Mutation：YES PGO S-1,2-PROPANEDIOL × 5 MG MAGNESIUM ION × 3 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；30% PROPANEDIOL, 0.1M HEPES PH 7.5, 20% PEG400
Resolution 2.50 Å R-free 0.225
4AAD Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in absence of metal ions at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3') Deposited 2011-12-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 2–153（152 aa）
Chain B 2–153（152 aa）
Mutation：YES Mutation：YES GOL GLYCEROL × 8 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；20% PEG300, 0.1M TRIS PH 8.5, 5% PEG8000, 10% GLYCEROL
Resolution 3.10 Å R-free 0.238
4AAE Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by AGCG from 5' to 3') Deposited 2011-12-01 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 2–154（153 aa）
Chain B 2–154（153 aa）
Mutation：YES Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 9.5；50% PEG400, 0.1M CHES PH 9.5, 0.2M NACL
Resolution 2.60 Å R-free 0.242
4AAF Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by TGCA from 5' to 3') Deposited 2011-12-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 2–153（152 aa）
Chain B 2–153（152 aa）
Mutation：YES Mutation：YES EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；35% METOH, 0.1M SODIUM CACODYLATE PH 6.5,
Resolution 2.50 Å R-free 0.247
4AAG Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in presence of Ca at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3') Deposited 2011-12-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 2–153（152 aa）
Chain B 2–153（152 aa）
Mutation：YES Mutation：YES CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；30% PEG400, 0.1M HEPES PH 7.5, 0.1M NACL
Resolution 2.80 Å R-free 0.250
4AQU Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of calcium Deposited 2012-04-19 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 2–153（152 aa） Fragment：RESIDUES 2-153
Chain B 2–153（152 aa） Fragment：RESIDUES 2-153
Not recorded CA CALCIUM ION × 2 GOL GLYCEROL × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.30 Å R-free 0.249
4AQX Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of magnesium Deposited 2012-04-19 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 2–153（152 aa） Fragment：RESIDUES 2-153
Chain B 2–153（152 aa） Fragment：RESIDUES 2-153
Not recorded GOL GLYCEROL × 5 MG MAGNESIUM ION × 3 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.20 Å R-free 0.242
6FB2 Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with its target DNA (Haemoglobin beta subunit gene) in the presence of Manganese Deposited 2017-12-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 2–154（153 aa）
Chain B 2–155（154 aa）
Not recorded MN MANGANESE (II) ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M calcium acetate, 0.1 M sodium acetate pH 4.6-5.4, 33-40% (v/v) 1,2-propanediol
Resolution 2.95 Å R-free 0.235
6FB7 Crystal Structure of the I-CreI Homing Endonuclease D75N variant in complex with its target DNA in the presence of Manganese Deposited 2017-12-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 2–153（152 aa）
Chain B 2–153（152 aa）
Not recorded MN MANGANESE (II) ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M calcium acetate, 0.1 M sodium acetate pH 4.6-5.4, 33-40% (v/v) 1,2-propanediol
Resolution 2.69 Å R-free 0.211
6FB8 Crystal Structure of the I-CreI Homing Endonuclease D75N variant in complex with an altered version of its target DNA at 5NNN region in the presence of Magnesium Deposited 2017-12-18 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 2–153（152 aa）
Chain B 2–153（152 aa）
Not recorded MG MAGNESIUM ION × 4 PGO S-1,2-PROPANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M calcium acetate, 0.1 M sodium acetate pH 4.6-5.4, 33-40% (v/v) 1,2-propanediol
Resolution 2.45 Å R-free 0.224
6FB9 Crystal Structure of the I-CreI Homing Endonuclease D75N variant in complex with an altered version of its target DNA at 5NNN region in the presence of Manganese Deposited 2017-12-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 2–153（152 aa）
Chain B 2–153（152 aa）
Not recorded MN MANGANESE (II) ION × 5 PGO S-1,2-PROPANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M calcium acetate, 0.1 M sodium acetate pH 4.6-5.4, 33-40% (v/v) 1,2-propanediol
Resolution 2.95 Å R-free 0.203

27 other PDB entries and 30 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name DNE1_CHLRE
Isoform —
PDB entities 3
Chains and sequence ranges Author chain A； PDBConstruct 1–163； UniProt 1–163 Author chain B； PDBConstruct 1–163； UniProt 1–163

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 1u0d

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 1u0d
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2. Structure Basics 2. Structure Basics

Entry ID entry_id1u0d
Deposition date deposition_date2004-07-13
Structure title titleY33H Mutant of Homing endonuclease I-CreI
Keywords keywordsDNA endonuclease I-CreI, PROTEIN/dna, HYDROLASE-dna COMPLEX; HYDROLASE/dna
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier24.73 Å
Radius of gyration Rg (electron density) rg_electron24.41 Å
Forward intensity I(0) i0200928000.00
Molecular weight molecular_weight99321.0 kDa
Excluded volume excluded_volume117340 ų
Envelope volume envelope_volume73415 ų
Hydration-shell volume shell_volume25886 ų
Envelope diameter envelope_diameter89.0 Å
Shell Rg shell_rg31.25 Å
Envelope Rg envelope_rg25.07 Å
Shape Rg shape_rg24.36 Å
Total Rg total_rg24.87 Å
Total atoms total_atoms6892
Residues n_residues704
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax88.9 Å
Rg (real space) rg_real24.95 Å
Rg uncertainty (real space) rg_real_error0.80 Å
I(0) (real space) i0_real2.0090e+08
I(0) uncertainty (real space) i0_real_error3.0490e+06
Rg (reciprocal space) rg_reciprocal24.90 Å
I(0) (reciprocal space) i0_reciprocal200900000.0000
Solution quality estimate total_estimate0.6601
Solution quality rating solution_quality REASONABLE a REASONABLE solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary25.4 Å
Skewness Skewness skewness0.571
Kurtosis Kurtosis kurtosis-0.164
Angular range angular_range— – 0.3200 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha6128000.0000
Real-space data points n_real_points64
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.625; Stabil: 1.000; Sysdev: 0.338; Positv: 1.000; Valcen: 0.692; Smooth: 0.996
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (3)

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7. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

Domain ID domain_idd1u0da_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.95 — Homing endonuclease-like
Superfamily Superfamily superfamilyd.95.2 — Homing endonucleases
Family Family familyd.95.2.1 — Group I mobile intron endonuclease
Domain ID domain_idd1u0db_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.95 — Homing endonuclease-like
Superfamily Superfamily superfamilyd.95.2 — Homing endonucleases
Family Family familyd.95.2.1 — Group I mobile intron endonuclease

CATH v4.4 (2 domains)

Domain ID domain_id1u0dA00
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology28 — Endonuclease I-creI
Homologous superfamily homologous superfamily10 — Homing endonucleases
Domain ID domain_id1u0dB00
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology28 — Endonuclease I-creI
Homologous superfamily homologous superfamily10 — Homing endonucleases
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8. Citations (1)

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9. Files and Curves (10)