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22fn

Cryo-EM structure of AsCas12a in complex with crDNA and RNA target

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

CRISPR-associated endonuclease Cas12a

Acidaminococcus sp. BV3L6

UniProt U2UMQ6

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Other combination Monomer Protein 1 DNA 1 RNA 1 ;RNA (5'-R(P*GP*AP*CP*AP*GP*CP*CP*CP*AP*CP*AP*UP*GP*GP*CP*AP*UP*UP*CP*CP*AP*CP*U)-3') ; × 1 DNA (41-MER) × 1 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
22fn 5b43 5kk5 5xh6 5xh7 8sfh 8sfi 8sfj 8sfl 8sfn 8sfo 8sfp

18 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name CS12A_ACISB
Isoform —
PDB entities 2
Chains and sequence ranges Author chain A； PDBConstruct 1–1307； UniProt 1–1307

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id22fn
Deposition date deposition_date2026-01-08
Structure title titleCryo-EM structure of AsCas12a in complex with crDNA and RNA target
Keywords keywordsCas12a, crDNA, RNA, DNA BINDING PROTEIN; DNA BINDING PROTEIN
Experimental Method methodELECTRON MICROSCOPY
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

22fn__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

22fn__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

22fn__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）37.48 Å
Rg (electron density)37.49 Å
Total Rg37.83 Å
Atom count11105
Residues1266
Excluded volume193700 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 22fn__assembly_1__model_1 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (3)

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7. Citations (1)