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26qv

Crystal structure of monoalkyl phthalate hydrolase from Rhodococcus sp. EG-5

Method: X-RAY DIFFRACTION Dmax: 225.2 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Mono-ethylhexylphthalate hydrolase

Rhodococcus sp. EG-5

UniProt A0A0U5ADH4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein homooligomer Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain A； UniProt 1–303 Chain B； UniProt 1–303 Not recorded No other associated polymer X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 3.00 Å R-free 0.294
2 Protein homooligomer Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain C； UniProt 1–303 Chain D； UniProt 1–303 Not recorded No other associated polymer X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 3.00 Å R-free 0.294
3 Protein homooligomer Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain E； UniProt 1–303 Chain H； UniProt 1–303 Not recorded No other associated polymer X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 3.00 Å R-free 0.294
4 Protein homooligomer Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain F； UniProt 1–303 Chain G； UniProt 1–303 Not recorded No other associated polymer X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 3.00 Å R-free 0.294
5 Protein homooligomer Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain I； UniProt 1–303 Chain J； UniProt 1–303 Not recorded No other associated polymer X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 3.00 Å R-free 0.294

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 26QV Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
9WTK Crystal structure of monoalkyl phthalate hydrolase in complex with MBP from Rhodococcus sp. EG-5 Deposited 2025-09-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–303（303 aa）
Not recorded PEG DI(HYDROXYETHYL)ETHER × 6 EDO 1,2-ETHANEDIOL × 4 PHT PHTHALIC ACID × 1 1BO 1-BUTANOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Magnesium sulfate heptahydrate, 20% w/v PEG 3350, pH 6.0
Resolution 2.80 Å R-free 0.286
9WTK Crystal structure of monoalkyl phthalate hydrolase in complex with MBP from Rhodococcus sp. EG-5 Deposited 2025-09-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 10 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain J 1–303（303 aa）
Not recorded PEG DI(HYDROXYETHYL)ETHER × 5 EDO 1,2-ETHANEDIOL × 5 PHT PHTHALIC ACID × 1 MLI MALONATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Magnesium sulfate heptahydrate, 20% w/v PEG 3350, pH 6.0
Resolution 2.80 Å R-free 0.286
9WTK Crystal structure of monoalkyl phthalate hydrolase in complex with MBP from Rhodococcus sp. EG-5 Deposited 2025-09-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–303（303 aa）
Not recorded PEG DI(HYDROXYETHYL)ETHER × 2 EDO 1,2-ETHANEDIOL × 13 PHT PHTHALIC ACID × 1 1BO 1-BUTANOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Magnesium sulfate heptahydrate, 20% w/v PEG 3350, pH 6.0
Resolution 2.80 Å R-free 0.286
9WTK Crystal structure of monoalkyl phthalate hydrolase in complex with MBP from Rhodococcus sp. EG-5 Deposited 2025-09-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 1–303（303 aa）
Not recorded PEG DI(HYDROXYETHYL)ETHER × 3 EDO 1,2-ETHANEDIOL × 5 PHT PHTHALIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Magnesium sulfate heptahydrate, 20% w/v PEG 3350, pH 6.0
Resolution 2.80 Å R-free 0.286
9WTK Crystal structure of monoalkyl phthalate hydrolase in complex with MBP from Rhodococcus sp. EG-5 Deposited 2025-09-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 1–303（303 aa）
Not recorded PEG DI(HYDROXYETHYL)ETHER × 7 EDO 1,2-ETHANEDIOL × 6 PHT PHTHALIC ACID × 1 1BO 1-BUTANOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Magnesium sulfate heptahydrate, 20% w/v PEG 3350, pH 6.0
Resolution 2.80 Å R-free 0.286
9WTK Crystal structure of monoalkyl phthalate hydrolase in complex with MBP from Rhodococcus sp. EG-5 Deposited 2025-09-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain E 1–303（303 aa）
Not recorded PEG DI(HYDROXYETHYL)ETHER × 7 EDO 1,2-ETHANEDIOL × 4 PHT PHTHALIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Magnesium sulfate heptahydrate, 20% w/v PEG 3350, pH 6.0
Resolution 2.80 Å R-free 0.286
9WTK Crystal structure of monoalkyl phthalate hydrolase in complex with MBP from Rhodococcus sp. EG-5 Deposited 2025-09-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain F 1–303（303 aa）
Not recorded PEG DI(HYDROXYETHYL)ETHER × 5 EDO 1,2-ETHANEDIOL × 7 PHT PHTHALIC ACID × 1 MLI MALONATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Magnesium sulfate heptahydrate, 20% w/v PEG 3350, pH 6.0
Resolution 2.80 Å R-free 0.286
9WTK Crystal structure of monoalkyl phthalate hydrolase in complex with MBP from Rhodococcus sp. EG-5 Deposited 2025-09-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 7 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain G 1–303（303 aa）
Not recorded PEG DI(HYDROXYETHYL)ETHER × 4 EDO 1,2-ETHANEDIOL × 8 PHT PHTHALIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Magnesium sulfate heptahydrate, 20% w/v PEG 3350, pH 6.0
Resolution 2.80 Å R-free 0.286
9WTK Crystal structure of monoalkyl phthalate hydrolase in complex with MBP from Rhodococcus sp. EG-5 Deposited 2025-09-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 8 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain H 1–303（303 aa）
Not recorded PEG DI(HYDROXYETHYL)ETHER × 1 EDO 1,2-ETHANEDIOL × 5 PHT PHTHALIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Magnesium sulfate heptahydrate, 20% w/v PEG 3350, pH 6.0
Resolution 2.80 Å R-free 0.286
9WTK Crystal structure of monoalkyl phthalate hydrolase in complex with MBP from Rhodococcus sp. EG-5 Deposited 2025-09-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 9 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain I 1–303（303 aa）
Not recorded PEG DI(HYDROXYETHYL)ETHER × 4 EDO 1,2-ETHANEDIOL × 4 PHT PHTHALIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Magnesium sulfate heptahydrate, 20% w/v PEG 3350, pH 6.0
Resolution 2.80 Å R-free 0.286

1 other PDB entries and 10 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name A0A0U5ADH4_9NOCA
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–303； UniProt 1–303 Author chain B； PDBConstruct 1–303； UniProt 1–303 Author chain C； PDBConstruct 1–303； UniProt 1–303 Author chain D； PDBConstruct 1–303； UniProt 1–303 Author chain E； PDBConstruct 1–303； UniProt 1–303 Author chain F； PDBConstruct 1–303； UniProt 1–303 Author chain G； PDBConstruct 1–303； UniProt 1–303 Author chain H； PDBConstruct 1–303； UniProt 1–303 Author chain I； PDBConstruct 1–303； UniProt 1–303 Author chain J； PDBConstruct 1–303； UniProt 1–303

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 26qv

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 26qv
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2. Structure Basics 2. Structure Basics

Entry ID entry_id26qv
Deposition date deposition_date2026-05-11
最后修订 last_revision2026-06-10
Structure title titleCrystal structure of monoalkyl phthalate hydrolase from Rhodococcus sp. EG-5
Keywords keywordsMono-butyl phthalate, HYDROLASE; HYDROLASE
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier68.78 Å
Radius of gyration Rg (electron density) rg_electron69.47 Å
Forward intensity I(0) i01322140000.00
Molecular weight molecular_weight310390.0 kDa
Excluded volume excluded_volume390240 ų
Envelope volume envelope_volume587760 ų
Hydration-shell volume shell_volume77557 ų
Envelope diameter envelope_diameter247.7 Å
Shell Rg shell_rg60.05 Å
Envelope Rg envelope_rg67.58 Å
Shape Rg shape_rg69.47 Å
Total Rg total_rg69.27 Å
Total atoms total_atoms43729
Residues n_residues2868
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax225.2 Å
Rg (real space) rg_real69.27 Å
Rg uncertainty (real space) rg_real_error2.03 Å
I(0) (real space) i0_real1.3210e+09
I(0) uncertainty (real space) i0_real_error2.9150e+07
Rg (reciprocal space) rg_reciprocal66.63 Å
I(0) (reciprocal space) i0_reciprocal1315000000.0000
Solution quality estimate total_estimate0.7990
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary49.0 Å
Skewness Skewness skewness0.575
Kurtosis Kurtosis kurtosis-0.323
Angular range angular_range— – 0.1150 Å−1
Current regularization parameter α current_alpha0.0009
Highest regularization parameter α highest_alpha37880000.0000
Real-space data points n_real_points24
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.815; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.734; Smooth: 0.206
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (2)

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8. Citations (1)

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9. Files and Curves (10)