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2ast

Crystal structure of Skp1-Skp2-Cks1 in complex with a p27 peptide

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

S-phase kinase-associated protein 1A

Homo sapiens

UniProt P63208

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 4 S-phase kinase-associated protein 2 × 1 （Q13309） Cyclin-dependent kinases regulatory subunit 1 × 1 （P61024） Cyclin-dependent kinase inhibitor 1B × 1 （P46527） BENZAMIDINE × 2 water × 3 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 8 S-phase kinase-associated protein 2 × 2 （Q13309） Cyclin-dependent kinases regulatory subunit 1 × 2 （P61024） Cyclin-dependent kinase inhibitor 1B × 2 （P46527） BENZAMIDINE × 4 water × 6 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 8 S-phase kinase-associated protein 2 × 2 （Q13309） Cyclin-dependent kinases regulatory subunit 1 × 2 （P61024） Cyclin-dependent kinase inhibitor 1B × 2 （P46527） BENZAMIDINE × 4 water × 6 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
5xyl

1 assemblies

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1fqv 1fqv 1fqv 1fqv 1fqv 1fqv 1fqv 1fqv 1fs1 1fs2 1ldk 1ldk

97 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name SKP1_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–159； UniProt 1–159

S-phase kinase-associated protein 2

Homo sapiens

UniProt Q13309

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 4 S-phase kinase-associated protein 1A × 1 （P63208） Cyclin-dependent kinases regulatory subunit 1 × 1 （P61024） Cyclin-dependent kinase inhibitor 1B × 1 （P46527） BENZAMIDINE × 2 water × 3 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 8 S-phase kinase-associated protein 1A × 2 （P63208） Cyclin-dependent kinases regulatory subunit 1 × 2 （P61024） Cyclin-dependent kinase inhibitor 1B × 2 （P46527） BENZAMIDINE × 4 water × 6 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 8 S-phase kinase-associated protein 1A × 2 （P63208） Cyclin-dependent kinases regulatory subunit 1 × 2 （P61024） Cyclin-dependent kinase inhibitor 1B × 2 （P46527） BENZAMIDINE × 4 water × 6 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1fqv 1fqv 1fqv 1fqv 1fqv 1fqv 1fqv 1fqv 1fs1 1fs2 1ldk 1ldk

39 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name SKP2_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–336； UniProt 89–424

Cyclin-dependent kinases regulatory subunit 1

Homo sapiens

UniProt P61024

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 4 S-phase kinase-associated protein 1A × 1 （P63208） S-phase kinase-associated protein 2 × 1 （Q13309） Cyclin-dependent kinase inhibitor 1B × 1 （P46527） BENZAMIDINE × 2 water × 3 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 8 S-phase kinase-associated protein 1A × 2 （P63208） S-phase kinase-associated protein 2 × 2 （Q13309） Cyclin-dependent kinase inhibitor 1B × 2 （P46527） BENZAMIDINE × 4 water × 6 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 8 S-phase kinase-associated protein 1A × 2 （P63208） S-phase kinase-associated protein 2 × 2 （Q13309） Cyclin-dependent kinase inhibitor 1B × 2 （P46527） BENZAMIDINE × 4 water × 6 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer
1dks 1dkt

2 assemblies

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1buh 2ass 2ass 2ass 2ast 2ast 2ast 4yc6 4yc6 4yc6 4yc6 7b5l

24 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name CKS1_HUMAN
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C； PDBConstruct 1–69； UniProt 5–73

Cyclin-dependent kinase inhibitor 1B

OrganismNot specified

UniProt P46527

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 4 S-phase kinase-associated protein 1A × 1 （P63208） S-phase kinase-associated protein 2 × 1 （Q13309） Cyclin-dependent kinases regulatory subunit 1 × 1 （P61024） BENZAMIDINE × 2 water × 3 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 8 S-phase kinase-associated protein 1A × 2 （P63208） S-phase kinase-associated protein 2 × 2 （Q13309） Cyclin-dependent kinases regulatory subunit 1 × 2 （P61024） BENZAMIDINE × 4 water × 6 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 8 S-phase kinase-associated protein 1A × 2 （P63208） S-phase kinase-associated protein 2 × 2 （Q13309） Cyclin-dependent kinases regulatory subunit 1 × 2 （P61024） BENZAMIDINE × 4 water × 6 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1h27 1jsu 2ast 2ast 2ast 5uq3 6ath 6p8e 6p8f 6p8g 7b5l 7b5m

19 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name CDN1B_HUMAN
Isoform —
PDB entities 4
Chains and sequence ranges Author chain D； PDBConstruct 1–10； UniProt 181–190

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id2ast
Deposition date deposition_date2005-08-24
Structure title titleCrystal structure of Skp1-Skp2-Cks1 in complex with a p27 peptide
Keywords keywordsSCF-substrate complex, LRR, CELL CYCLE, PROTEIN TURNOVER COMPLEX, LIGASE-LIGASE INHIBITOR complex; LIGASE/LIGASE INHIBITOR
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2ast__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2ast__assembly_1__model_1 | I(q)

10-2 10-1 106 107 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

2ast__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）29.64 Å
Rg (electron density)29.30 Å
Total Rg29.94 Å
Atom count4395
Residues545
Excluded volume78656 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 2ast__assembly_1__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 2ast__assembly_2__model_1 octameric （8） Success 4.1.3-1-20251215 (887e7ef) View Download
3 1 2ast__assembly_3__model_1 octameric （8） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (6)

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6. Fold Classification (SCOP + CATH) 6 domains

SCOP 2.08 (3 domains)

Domain ID domain_idd2astb1
Class classa — All alpha proteins
Fold Fold folda.158 — F-box domain
Superfamily Superfamily superfamilya.158.1 — F-box domain
Family Family familya.158.1.1 — F-box domain
Domain ID domain_idd2astb2
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.10 — Leucine-rich repeat, LRR (right-handed beta-alpha superhelix)
Superfamily Superfamily superfamilyc.10.1 — RNI-like
Family Family familyc.10.1.3 — Cyclin A/CDK2-associated p19, Skp2
Domain ID domain_idd2astc_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.97 — Cell cycle regulatory proteins
Superfamily Superfamily superfamilyd.97.1 — Cell cycle regulatory proteins
Family Family familyd.97.1.1 — Cell cycle regulatory proteins

CATH v4.4 (3 domains)

Domain ID domain_id2astA00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology710 — Potassium Channel Kv1.1; Chain A
Homologous superfamily homologous superfamily10 — Potassium Channel Kv1.1; Chain A
Domain ID domain_id2astB00
Class class3 — Alpha Beta
Architecture architecture80 — Alpha-Beta Horseshoe
Topology topology10 — Leucine-rich repeat, LRR (right-handed beta-alpha superhelix)
Homologous superfamily homologous superfamily10 — Ribonuclease Inhibitor
Domain ID domain_id2astC00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology170 — Cyclin-Dependent Kinase Subunit Type 2
Homologous superfamily homologous superfamily10 — Cyclin-dependent kinase, regulatory subunit
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7. Citations (1)