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2bvw

CELLOBIOHYDROLASE II (CEL6A) FROM HUMICOLA INSOLENS IN COMPLEX WITH GLUCOSE AND CELLOTETRAOSE

Method: X-RAY DIFFRACTION Dmax: 110.5 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

CELLOBIOHYDROLASE II

Humicola insolens

UniProt Q9C1S9

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Monomer Protein × 1 其他Polymer 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 115–476 Fragment：CATALYTIC CORE DOMAIN beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 BGC beta-D-glucopyranose × 1 GOL GLYCEROL × 6 X-RAY DIFFRACTION X-ray crystallization conditions：pH 4.6；pH 4.6 Resolution 1.70 Å R-free 0.226
2 Other combination Monomer Protein × 1 其他Polymer 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain B； UniProt 115–476 Fragment：CATALYTIC CORE DOMAIN beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-alpha-D-glucopyranose × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 BGC beta-D-glucopyranose × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION X-ray crystallization conditions：pH 4.6；pH 4.6 Resolution 1.70 Å R-free 0.226

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 2BVW Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1BVW CELLOBIOHYDROLASE II (CEL6A) FROM HUMICOLA INSOLENS Deposited 1998-09-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 91–450（360 aa） Fragment：CATALYTIC CORE DOMAIN RESIDUES 91-450
Not recorded MAN alpha-D-mannopyranose × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 MG MAGNESIUM ION × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；PROTEIN WAS CONCENTRATED TO 20 MG/ML IN WATER. CRYSTALLISATION IN 200MM MAGNESIUM ACETATE IN 100MM TRIETHANOLAMINE BUFFER AT PH 7.0. PRECIPITANT WAS 22%. POLYETHYLENE GLYCOL 8000.
Resolution 1.92 Å R-free 0.210
4I5R Crystal structure of a fungal chimeric cellobiohydrolase Cel6A Deposited 2012-11-28 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 117–161（45 aa）
Not recorded PGE TRIETHYLENE GLYCOL × 1 PG4 TETRAETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 5 ACT ACETATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.75；pH 5.75, VAPOR DIFFUSION, SITTING DROP
Resolution 1.50 Å R-free 0.181
4I5U Crystal structure of a fungal chimeric cellobiohydrolase Cel6A Deposited 2012-11-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 117–161（45 aa）
Not recorded ACT ACETATE ION × 2 EDO 1,2-ETHANEDIOL × 10 PG4 TETRAETHYLENE GLYCOL × 1 PGE TRIETHYLENE GLYCOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；pH 6.0, VAPOR DIFFUSION, SITTING DROP
Resolution 1.22 Å R-free 0.158

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q9C1S9_HUMIN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–362； UniProt 115–476 Author chain B； PDBConstruct 1–362； UniProt 115–476

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 2bvw

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 2bvw
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2. Structure Basics 2. Structure Basics

Entry ID entry_id2bvw
Deposition date deposition_date1999-02-18
Structure title titleCELLOBIOHYDROLASE II (CEL6A) FROM HUMICOLA INSOLENS IN COMPLEX WITH GLUCOSE AND CELLOTETRAOSE
Keywords keywordsHYDROLASE, CELLULOSE DEGRADATION, CELLOBIOHYDROLASE, CELLULASE, GLYCOSIDE HYDROLASE FAMILY 6; HYDROLASE
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier35.08 Å
Radius of gyration Rg (electron density) rg_electron34.74 Å
Forward intensity I(0) i0106875000.00
Molecular weight molecular_weight82090.0 kDa
Excluded volume excluded_volume102000 ų
Envelope volume envelope_volume126230 ų
Hydration-shell volume shell_volume30345 ų
Envelope diameter envelope_diameter111.4 Å
Shell Rg shell_rg41.23 Å
Envelope Rg envelope_rg33.94 Å
Shape Rg shape_rg34.72 Å
Total Rg total_rg35.24 Å
Total atoms total_atoms5795
Residues n_residues721
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax110.5 Å
Rg (real space) rg_real35.28 Å
Rg uncertainty (real space) rg_real_error1.02 Å
I(0) (real space) i0_real1.0690e+08
I(0) uncertainty (real space) i0_real_error1.8580e+06
Rg (reciprocal space) rg_reciprocal35.17 Å
I(0) (reciprocal space) i0_reciprocal106900000.0000
Solution quality estimate total_estimate0.7639
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks3
Primary peak position r_peak_primary27.0 Å
Skewness Skewness skewness0.304
Kurtosis Kurtosis kurtosis-0.962
Angular range angular_range— – 0.2250 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha37570000.0000
Real-space data points n_real_points46
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.517; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.728; Smooth: 0.649
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (7)

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7. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

Domain ID domain_idd2bvwa_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.6 — 7-stranded beta/alpha barrel
Superfamily Superfamily superfamilyc.6.1 — Glycosyl hydrolases family 6, cellulases
Family Family familyc.6.1.1 — Glycosyl hydrolases family 6, cellulases
Domain ID domain_idd2bvwb_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.6 — 7-stranded beta/alpha barrel
Superfamily Superfamily superfamilyc.6.1 — Glycosyl hydrolases family 6, cellulases
Family Family familyc.6.1.1 — Glycosyl hydrolases family 6, cellulases

CATH v4.4 (2 domains)

Domain ID domain_id2bvwA00
Class class3 — Alpha Beta
Architecture architecture20 — Alpha-Beta Barrel
Topology topology20 — TIM Barrel
Homologous superfamily homologous superfamily40 — 1, 4-beta cellobiohydrolase
Domain ID domain_id2bvwB00
Class class3 — Alpha Beta
Architecture architecture20 — Alpha-Beta Barrel
Topology topology20 — TIM Barrel
Homologous superfamily homologous superfamily40 — 1, 4-beta cellobiohydrolase
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8. Citations (1)

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9. Files and Curves (10)