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1bvw

CELLOBIOHYDROLASE II (CEL6A) FROM HUMICOLA INSOLENS

Method: X-RAY DIFFRACTION Dmax: 62.5 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

PROTEIN (CELLOBIOHYDROLASE II)

Humicola insolens

UniProt Q9C1S9

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 91–450 Fragment：CATALYTIC CORE DOMAIN RESIDUES 91-450 MAN alpha-D-mannopyranose × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 MG MAGNESIUM ION × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION X-ray crystallization conditions：pH 7；PROTEIN WAS CONCENTRATED TO 20 MG/ML IN WATER. CRYSTALLISATION IN 200MM MAGNESIUM ACETATE IN 100MM TRIETHANOLAMINE BUFFER AT PH 7.0. PRECIPITANT WAS 22%. POLYETHYLENE GLYCOL 8000. Resolution 1.92 Å R-free 0.210

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 1BVW Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2BVW CELLOBIOHYDROLASE II (CEL6A) FROM HUMICOLA INSOLENS IN COMPLEX WITH GLUCOSE AND CELLOTETRAOSE Deposited 1999-02-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric
Chain A 115–476（362 aa） Fragment：CATALYTIC CORE DOMAIN
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 BGC beta-D-glucopyranose × 1 GOL GLYCEROL × 6 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；pH 4.6
Resolution 1.70 Å R-free 0.226
2BVW CELLOBIOHYDROLASE II (CEL6A) FROM HUMICOLA INSOLENS IN COMPLEX WITH GLUCOSE AND CELLOTETRAOSE Deposited 1999-02-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric
Chain B 115–476（362 aa） Fragment：CATALYTIC CORE DOMAIN
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 BGC beta-D-glucopyranose × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；pH 4.6
Resolution 1.70 Å R-free 0.226
4I5R Crystal structure of a fungal chimeric cellobiohydrolase Cel6A Deposited 2012-11-28 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 117–161（45 aa）
Not recorded PGE TRIETHYLENE GLYCOL × 1 PG4 TETRAETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 5 ACT ACETATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.75；pH 5.75, VAPOR DIFFUSION, SITTING DROP
Resolution 1.50 Å R-free 0.181
4I5U Crystal structure of a fungal chimeric cellobiohydrolase Cel6A Deposited 2012-11-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 117–161（45 aa）
Not recorded ACT ACETATE ION × 2 EDO 1,2-ETHANEDIOL × 10 PG4 TETRAETHYLENE GLYCOL × 1 PGE TRIETHYLENE GLYCOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；pH 6.0, VAPOR DIFFUSION, SITTING DROP
Resolution 1.22 Å R-free 0.158

3 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q9C1S9_HUMIN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–360； UniProt 91–450

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 1bvw

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 1bvw
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2. Structure Basics 2. Structure Basics

Entry ID entry_id1bvw
Deposition date deposition_date1998-09-19
Structure title titleCELLOBIOHYDROLASE II (CEL6A) FROM HUMICOLA INSOLENS
Keywords keywordsCELLULOSE DEGRADATION, CELLOBIOHYDROLASE, CELLULASE, GLYCOSIDE HYDROLASE FAMILY 6, HYDROLASE; HYDROLASE
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier20.46 Å
Radius of gyration Rg (electron density) rg_electron19.31 Å
Forward intensity I(0) i028324500.00
Molecular weight molecular_weight40340.0 kDa
Excluded volume excluded_volume50138 ų
Envelope volume envelope_volume55739 ų
Hydration-shell volume shell_volume23341 ų
Envelope diameter envelope_diameter64.2 Å
Shell Rg shell_rg26.52 Å
Envelope Rg envelope_rg19.47 Å
Shape Rg shape_rg19.27 Å
Total Rg total_rg20.29 Å
Total atoms total_atoms2850
Residues n_residues360
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax62.5 Å
Rg (real space) rg_real20.29 Å
Rg uncertainty (real space) rg_real_error0.23 Å
I(0) (real space) i0_real2.8320e+07
I(0) uncertainty (real space) i0_real_error2.8780e+05
Rg (reciprocal space) rg_reciprocal20.32 Å
I(0) (reciprocal space) i0_reciprocal28320000.0000
Solution quality estimate total_estimate0.8962
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary27.7 Å
Skewness Skewness skewness0.050
Kurtosis Kurtosis kurtosis-0.474
Angular range angular_range— – 0.3900 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha6862000.0000
Real-space data points n_real_points71
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.899; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.971; Smooth: 0.979
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (6)

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7. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

Domain ID domain_idd1bvwa_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.6 — 7-stranded beta/alpha barrel
Superfamily Superfamily superfamilyc.6.1 — Glycosyl hydrolases family 6, cellulases
Family Family familyc.6.1.1 — Glycosyl hydrolases family 6, cellulases

CATH v4.4 (1 domains)

Domain ID domain_id1bvwA00
Class class3 — Alpha Beta
Architecture architecture20 — Alpha-Beta Barrel
Topology topology20 — TIM Barrel
Homologous superfamily homologous superfamily40 — 1, 4-beta cellobiohydrolase
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8. Citations (1)

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9. Files and Curves (10)