2eul

Structure of the transcription factor Gfh1.

Method: X-RAY DIFFRACTION D_max: 96.9 Å Quality: REASONABLE

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	2eul
沉积日期 deposition_date	2005-10-28
结构标题 title	Structure of the transcription factor Gfh1.
关键词 keywords	;Gfh1, transcription factor, RNA polymerase, RIKEN Structural Genomics/Proteomics Initiative, RSGI, Structural Genomics, TRANSCRIPTION ;; TRANSCRIPTION
实验方法 method	X-RAY DIFFRACTION

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	30.49 Å
回转半径 Rg (电子) rg_electron	29.98 Å
零角强度 I(0) i0	91268300.00
分子量 molecular_weight	70328.0 kDa
排除体积 excluded_volume	86072 Å³
包络体积 envelope_volume	119520 Å³
水化壳体积 shell_volume	33856 Å³
包络直径 envelope_diameter	105.0 Å
壳层 Rg shell_rg	36.29 Å
包络 Rg envelope_rg	29.73 Å
形状 Rg shape_rg	30.06 Å
总 Rg total_rg	30.31 Å
总原子数 total_atoms	4833
残基数 n_residues	624
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	96.9 Å
Rg (实空间) rg_real	30.41 Å
Rg 误差 (实空间) rg_real_error	0.66 Å
I(0) (实空间) i0_real	9.1270e+07
I(0) 误差 (实空间) i0_real_error	1.3450e+06
Rg (倒空间) rg_reciprocal	30.45 Å
I(0) (倒空间) i0_reciprocal	91270000.0000
解质量估计 total_estimate	0.6777
解质量评级 solution_quality	REASONABLE a REASONABLE solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	39.1 Å
偏度 Skewness skewness	0.245
峰度 Kurtosis kurtosis	-0.308
角度范围 angular_range	— – 0.2600 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	7694000.0000
实空间数据点数 n_real_points	53
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.881; Stabil: 1.000; Sysdev: 0.077; Positv: 1.000; Valcen: 1.000; Smooth: 0.934

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (3)

Entity 1 polymer

描述 description	anti-cleavage anti-GreA transcription factor Gfh1
分子量 formula_weight	17206.5 kDa
来源方法 src_method	man
拷贝数 entity_count	4

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D
原子数 atom_count	5219

MAREVKLTKAGYERLMQQLERERERLQEATKILQELMESSDDYDDSGLEAAKQEKARIEARIDSLEDILSRAVILEEGSGEVIGLGSVVELEDPLSGERLSVQVVSPAEANVLDTPMKISDASPMGKALLGHRVGDVLSLDTPKGRREFRVVAIHG

来源 Source

来源生物 organism	Thermus thermophilus
NCBI 分类编号 ncbi_taxonomy_id	300852
宿主生物 host_organism	Escherichia coli

Entity 2 non-polymer

描述 description	ZINC ION
分子量 formula_weight	65.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	25

Entity 3 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	386

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6. 折叠分类 (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

结构域编号 domain_id	d2eula1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.2 — Long alpha-hairpin
超家族 Superfamily superfamily	a.2.1 — GreA transcript cleavage protein, N-terminal domain
家族 Family family	a.2.1.1 — GreA transcript cleavage protein, N-terminal domain

结构域编号 domain_id	d2eula2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.26 — FKBP-like
超家族 Superfamily superfamily	d.26.1 — FKBP-like
家族 Family family	d.26.1.2 — GreA transcript cleavage factor, C-terminal domain

结构域编号 domain_id	d2eulb1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.2 — Long alpha-hairpin
超家族 Superfamily superfamily	a.2.1 — GreA transcript cleavage protein, N-terminal domain
家族 Family family	a.2.1.1 — GreA transcript cleavage protein, N-terminal domain

结构域编号 domain_id	d2eulb2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.26 — FKBP-like
超家族 Superfamily superfamily	d.26.1 — FKBP-like
家族 Family family	d.26.1.2 — GreA transcript cleavage factor, C-terminal domain

结构域编号 domain_id	d2eulc1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.2 — Long alpha-hairpin
超家族 Superfamily superfamily	a.2.1 — GreA transcript cleavage protein, N-terminal domain
家族 Family family	a.2.1.1 — GreA transcript cleavage protein, N-terminal domain

结构域编号 domain_id	d2eulc2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.26 — FKBP-like
超家族 Superfamily superfamily	d.26.1 — FKBP-like
家族 Family family	d.26.1.2 — GreA transcript cleavage factor, C-terminal domain

结构域编号 domain_id	d2euld1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.2 — Long alpha-hairpin
超家族 Superfamily superfamily	a.2.1 — GreA transcript cleavage protein, N-terminal domain
家族 Family family	a.2.1.1 — GreA transcript cleavage protein, N-terminal domain

结构域编号 domain_id	d2euld2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.26 — FKBP-like
超家族 Superfamily superfamily	d.26.1 — FKBP-like
家族 Family family	d.26.1.2 — GreA transcript cleavage factor, C-terminal domain

CATH v4.4 (8 domains)

结构域编号 domain_id	2eulA01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	287 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily	180 — Transcription elongation factor, GreA/GreB, N-terminal domain

结构域编号 domain_id	2eulA02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	10 — Roll
拓扑 Topology topology	50 — Chitinase A; domain 3
同源超家族 H-superfamily homologous superfamily	30 — Transcription elongation factor, GreA/GreB, C-terminal domain

结构域编号 domain_id	2eulB01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	287 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily	180 — Transcription elongation factor, GreA/GreB, N-terminal domain

结构域编号 domain_id	2eulB02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	10 — Roll
拓扑 Topology topology	50 — Chitinase A; domain 3
同源超家族 H-superfamily homologous superfamily	30 — Transcription elongation factor, GreA/GreB, C-terminal domain

结构域编号 domain_id	2eulC01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	287 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily	180 — Transcription elongation factor, GreA/GreB, N-terminal domain

结构域编号 domain_id	2eulC02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	10 — Roll
拓扑 Topology topology	50 — Chitinase A; domain 3
同源超家族 H-superfamily homologous superfamily	30 — Transcription elongation factor, GreA/GreB, C-terminal domain

结构域编号 domain_id	2eulD01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	287 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily	180 — Transcription elongation factor, GreA/GreB, N-terminal domain

结构域编号 domain_id	2eulD02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	10 — Roll
拓扑 Topology topology	50 — Chitinase A; domain 3
同源超家族 H-superfamily homologous superfamily	30 — Transcription elongation factor, GreA/GreB, C-terminal domain

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7. 引用文献 (1)

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晶胞边长 a length_a	59.291 Å
晶胞边长 b length_b	59.291 Å
晶胞边长 c length_c	218.876 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	16

空间群 space_group	P 43
空间群编号 int_tables_number	78
晶系 crystal_system	—

分辨率 resolution	2.400 Å
最低分辨率 resolution_low	30.000 Å
R 因子 r_factor	0.2060
R_work r_work	0.2060
R_free r_free	0.2450

fit	/app/data/saxs_computation/eu/2eul/2eul.fit
pddf	/app/data/pr_computation/eu/2eul/2eul.pr.out
profile	/app/data/saxs_computation/eu/2eul/2eul.dat