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3aoh

RNA polymerase-Gfh1 complex (Crystal type 1)

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

DNA-directed RNA polymerase subunit alpha

OrganismNot specified

UniProt Q5SHR6

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Other combination Heteromer Protein 6 DNA 1 RNA 1 DNA-directed RNA polymerase subunit beta × 1 （Q8RQE9） ;DNA-directed RNA polymerase subunit beta' ; × 1 （Q8RQE8） DNA-directed RNA polymerase subunit omega × 1 （Q8RQE7） ;DNA (5'-D(*GP*GP*TP*CP*TP*GP*TP*AP*TP*CP*AP*CP*GP*AP*GP*CP*CP*AP*CP*CP*GP*CP*CP*GP*CP*AP*T)-3') ; × 1 ;RNA (5'-R(*CP*CP*CP*CP*GP*GP*AP*AP*GP*AP*UP*CP*AP*UP*CP*UP*UP*CP*CP*GP*GP*GP*GP*GP*AP*U*GP*CP*GP*GP*CP*GP*G)-3') ; × 1 Anti-cleavage anti-GreA transcription factor Gfh1 × 1 （Q5SJG6） ZINC ION × 1 MAGNESIUM ION × 1 Consistent with all polymers
2 Protein heterocomplex Heteromer Protein 6 DNA-directed RNA polymerase subunit beta × 1 （Q8RQE9） ;DNA-directed RNA polymerase subunit beta' ; × 1 （Q8RQE8） DNA-directed RNA polymerase subunit omega × 1 （Q8RQE7） Anti-cleavage anti-GreA transcription factor Gfh1 × 1 （Q5SJG6） ZINC ION × 1 MAGNESIUM ION × 1 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 6 DNA-directed RNA polymerase subunit beta × 1 （Q8RQE9） ;DNA-directed RNA polymerase subunit beta' ; × 1 （Q8RQE8） DNA-directed RNA polymerase subunit omega × 1 （Q8RQE7） Anti-cleavage anti-GreA transcription factor Gfh1 × 1 （Q5SJG6） ZINC ION × 1 MAGNESIUM ION × 1 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1zyr 1zyr 2a6e 2a6e 2a6h 2a6h 2cw0 2cw0 2o5i 2o5i 2o5j 2o5j

95 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name RPOA_THET8
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–315； UniProt 1–315 Author chain B； PDBConstruct 1–315； UniProt 1–315 Author chain F； PDBConstruct 1–315； UniProt 1–315 Author chain G； PDBConstruct 1–315； UniProt 1–315 Author chain K； PDBConstruct 1–315； UniProt 1–315 Author chain L； PDBConstruct 1–315； UniProt 1–315

DNA-directed RNA polymerase subunit beta

OrganismNot specified

UniProt Q8RQE9

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Other combination Heteromer Protein 6 DNA 1 RNA 1 DNA-directed RNA polymerase subunit alpha × 2 （Q5SHR6） ;DNA-directed RNA polymerase subunit beta' ; × 1 （Q8RQE8） DNA-directed RNA polymerase subunit omega × 1 （Q8RQE7） ;DNA (5'-D(*GP*GP*TP*CP*TP*GP*TP*AP*TP*CP*AP*CP*GP*AP*GP*CP*CP*AP*CP*CP*GP*CP*CP*GP*CP*AP*T)-3') ; × 1 ;RNA (5'-R(*CP*CP*CP*CP*GP*GP*AP*AP*GP*AP*UP*CP*AP*UP*CP*UP*UP*CP*CP*GP*GP*GP*GP*GP*AP*U*GP*CP*GP*GP*CP*GP*G)-3') ; × 1 Anti-cleavage anti-GreA transcription factor Gfh1 × 1 （Q5SJG6） ZINC ION × 1 MAGNESIUM ION × 1 Consistent with all polymers
2 Protein heterocomplex Heteromer Protein 6 DNA-directed RNA polymerase subunit alpha × 2 （Q5SHR6） ;DNA-directed RNA polymerase subunit beta' ; × 1 （Q8RQE8） DNA-directed RNA polymerase subunit omega × 1 （Q8RQE7） Anti-cleavage anti-GreA transcription factor Gfh1 × 1 （Q5SJG6） ZINC ION × 1 MAGNESIUM ION × 1 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 6 DNA-directed RNA polymerase subunit alpha × 2 （Q5SHR6） ;DNA-directed RNA polymerase subunit beta' ; × 1 （Q8RQE8） DNA-directed RNA polymerase subunit omega × 1 （Q8RQE7） Anti-cleavage anti-GreA transcription factor Gfh1 × 1 （Q5SJG6） ZINC ION × 1 MAGNESIUM ION × 1 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1smy 1smy 1zyr 1zyr 2a68 2a68 2a69 2a69 2a6e 2a6e 2a6h 2a6h

147 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name RPOB_THET8
Isoform —
PDB entities 2
Chains and sequence ranges Author chain C； PDBConstruct 1–1119； UniProt 1–1119 Author chain H； PDBConstruct 1–1119； UniProt 1–1119 Author chain M； PDBConstruct 1–1119； UniProt 1–1119

;DNA-directed RNA polymerase subunit beta' ;

OrganismNot specified

UniProt Q8RQE8

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Other combination Heteromer Protein 6 DNA 1 RNA 1 DNA-directed RNA polymerase subunit alpha × 2 （Q5SHR6） DNA-directed RNA polymerase subunit beta × 1 （Q8RQE9） DNA-directed RNA polymerase subunit omega × 1 （Q8RQE7） ;DNA (5'-D(*GP*GP*TP*CP*TP*GP*TP*AP*TP*CP*AP*CP*GP*AP*GP*CP*CP*AP*CP*CP*GP*CP*CP*GP*CP*AP*T)-3') ; × 1 ;RNA (5'-R(*CP*CP*CP*CP*GP*GP*AP*AP*GP*AP*UP*CP*AP*UP*CP*UP*UP*CP*CP*GP*GP*GP*GP*GP*AP*U*GP*CP*GP*GP*CP*GP*G)-3') ; × 1 Anti-cleavage anti-GreA transcription factor Gfh1 × 1 （Q5SJG6） ZINC ION × 1 MAGNESIUM ION × 1 Consistent with all polymers
2 Protein heterocomplex Heteromer Protein 6 DNA-directed RNA polymerase subunit alpha × 2 （Q5SHR6） DNA-directed RNA polymerase subunit beta × 1 （Q8RQE9） DNA-directed RNA polymerase subunit omega × 1 （Q8RQE7） Anti-cleavage anti-GreA transcription factor Gfh1 × 1 （Q5SJG6） ZINC ION × 1 MAGNESIUM ION × 1 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 6 DNA-directed RNA polymerase subunit alpha × 2 （Q5SHR6） DNA-directed RNA polymerase subunit beta × 1 （Q8RQE9） DNA-directed RNA polymerase subunit omega × 1 （Q8RQE7） Anti-cleavage anti-GreA transcription factor Gfh1 × 1 （Q5SJG6） ZINC ION × 1 MAGNESIUM ION × 1 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1smy 1smy 1zyr 1zyr 2a68 2a68 2a69 2a69 2a6e 2a6e 2a6h 2a6h

133 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name RPOC_THET8
Isoform —
PDB entities 3
Chains and sequence ranges Author chain D； PDBConstruct 1–1524； UniProt 1–1524 Author chain I； PDBConstruct 1–1524； UniProt 1–1524 Author chain N； PDBConstruct 1–1524； UniProt 1–1524

DNA-directed RNA polymerase subunit omega

OrganismNot specified

UniProt Q8RQE7

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Other combination Heteromer Protein 6 DNA 1 RNA 1 DNA-directed RNA polymerase subunit alpha × 2 （Q5SHR6） DNA-directed RNA polymerase subunit beta × 1 （Q8RQE9） ;DNA-directed RNA polymerase subunit beta' ; × 1 （Q8RQE8） ;DNA (5'-D(*GP*GP*TP*CP*TP*GP*TP*AP*TP*CP*AP*CP*GP*AP*GP*CP*CP*AP*CP*CP*GP*CP*CP*GP*CP*AP*T)-3') ; × 1 ;RNA (5'-R(*CP*CP*CP*CP*GP*GP*AP*AP*GP*AP*UP*CP*AP*UP*CP*UP*UP*CP*CP*GP*GP*GP*GP*GP*AP*U*GP*CP*GP*GP*CP*GP*G)-3') ; × 1 Anti-cleavage anti-GreA transcription factor Gfh1 × 1 （Q5SJG6） ZINC ION × 1 MAGNESIUM ION × 1 Consistent with all polymers
2 Protein heterocomplex Heteromer Protein 6 DNA-directed RNA polymerase subunit alpha × 2 （Q5SHR6） DNA-directed RNA polymerase subunit beta × 1 （Q8RQE9） ;DNA-directed RNA polymerase subunit beta' ; × 1 （Q8RQE8） Anti-cleavage anti-GreA transcription factor Gfh1 × 1 （Q5SJG6） ZINC ION × 1 MAGNESIUM ION × 1 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 6 DNA-directed RNA polymerase subunit alpha × 2 （Q5SHR6） DNA-directed RNA polymerase subunit beta × 1 （Q8RQE9） ;DNA-directed RNA polymerase subunit beta' ; × 1 （Q8RQE8） Anti-cleavage anti-GreA transcription factor Gfh1 × 1 （Q5SJG6） ZINC ION × 1 MAGNESIUM ION × 1 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1zyr 1zyr 2a6e 2a6e 2a6h 2a6h 2be5 2be5 2cw0 2cw0 2o5i 2o5i

113 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name RPOZ_THET8
Isoform —
PDB entities 4
Chains and sequence ranges Author chain E； PDBConstruct 1–99； UniProt 1–99 Author chain J； PDBConstruct 1–99； UniProt 1–99 Author chain O； PDBConstruct 1–99； UniProt 1–99

Anti-cleavage anti-GreA transcription factor Gfh1

Thermus thermophilus

UniProt Q5SJG6

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Other combination Heteromer Protein 6 DNA 1 RNA 1 DNA-directed RNA polymerase subunit alpha × 2 （Q5SHR6） DNA-directed RNA polymerase subunit beta × 1 （Q8RQE9） ;DNA-directed RNA polymerase subunit beta' ; × 1 （Q8RQE8） DNA-directed RNA polymerase subunit omega × 1 （Q8RQE7） ;DNA (5'-D(*GP*GP*TP*CP*TP*GP*TP*AP*TP*CP*AP*CP*GP*AP*GP*CP*CP*AP*CP*CP*GP*CP*CP*GP*CP*AP*T)-3') ; × 1 ;RNA (5'-R(*CP*CP*CP*CP*GP*GP*AP*AP*GP*AP*UP*CP*AP*UP*CP*UP*UP*CP*CP*GP*GP*GP*GP*GP*AP*U*GP*CP*GP*GP*CP*GP*G)-3') ; × 1 ZINC ION × 1 MAGNESIUM ION × 1 Consistent with all polymers
2 Protein heterocomplex Heteromer Protein 6 DNA-directed RNA polymerase subunit alpha × 2 （Q5SHR6） DNA-directed RNA polymerase subunit beta × 1 （Q8RQE9） ;DNA-directed RNA polymerase subunit beta' ; × 1 （Q8RQE8） DNA-directed RNA polymerase subunit omega × 1 （Q8RQE7） ZINC ION × 1 MAGNESIUM ION × 1 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 6 DNA-directed RNA polymerase subunit alpha × 2 （Q5SHR6） DNA-directed RNA polymerase subunit beta × 1 （Q8RQE9） ;DNA-directed RNA polymerase subunit beta' ; × 1 （Q8RQE8） DNA-directed RNA polymerase subunit omega × 1 （Q8RQE7） ZINC ION × 1 MAGNESIUM ION × 1 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer
2eul 2eul

2 assemblies

Homotetramer
2eul 2eul 2eul 2eul 2eul

5 assemblies

Other homooligomers

None

Heteromeric or nucleic-acid complex
3aoh 3aoh 3aoh 3aoi 3aoi 3aoi

6 assemblies

View Construct and Data Evidence
UniProt name Q5SJG6_THET8
Isoform —
PDB entities 7
Chains and sequence ranges Author chain X； PDBConstruct 1–156； UniProt 1–156 Author chain Y； PDBConstruct 1–156； UniProt 1–156 Author chain Z； PDBConstruct 1–156； UniProt 1–156

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id3aoh
Deposition date deposition_date2010-09-28
Structure title titleRNA polymerase-Gfh1 complex (Crystal type 1)
Keywords keywordsStructural Genomics, RIKEN Structural Genomics/Proteomics Initiative, RSGI, Transcription, TRANSFERASE-DNA-RNA complex; Transcription, TRANSFERASE/DNA/RNA
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

3aoh__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

3aoh__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

3aoh__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）49.68 Å
Rg (electron density)49.84 Å
Total Rg49.96 Å
Atom count25099
Residues3159
Excluded volume447510 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 3aoh__assembly_1__model_1 octameric （8） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 3aoh__assembly_2__model_1 hexameric （6） Success 4.1.3-1-20251215 (887e7ef) View Download
3 1 3aoh__assembly_3__model_1 hexameric （6） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (9)

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7. Citations (1)