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2fk4

Solution structure of the C-terminal zinc binding domain of the HPV16 E6 oncoprotein

Method: SOLUTION NMR Dmax: 43.3 Å Quality: REASONABLE
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Protein E6

Human papillomavirus type 16

UniProt P03126

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 87–158 Fragment：C-TERMINAL DOMAIN Mutation：C4S, C20S, C34S, C63S ZN ZINC ION × 1 SOLUTION NMR NMR measurement conditions：pH 6.8；288 K；Ionic strength (raw mmCIF value) 50 mM；Pressure ambient NMR measurement conditions：pH 6.8；288 K；Ionic strength (raw mmCIF value) 50 mM；Pressure ambient NMR sample composition：1.0 mM E6C U-15N; 20 mM TRIS-HCl, 50 mM NaCl, 1mM DTT | 90% H2O/10% D2O NMR sample composition：1.0 mM E6C, 20 mM TRIS-HCl, 50 mM NaCl, 1mM DTT | 90% H2O/10% D2O Resolution not provided

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 2FK4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2KPL MAGI-1 PDZ1 / E6CT Deposited 2009-10-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 148–157（10 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.8；295 K；Pressure ambient
NMR sample composition 0.2-0.6mM MAGI-1 PDZ1-1, 0.02-0.10mM sodium phosphate-2, 50mM sodium chloride-3, 2mM DTT-4, 0.6-1.8mM E6CT-5, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 0.2-0.6mM [U-15N] MAGI-1 PDZ1-6, 0.02-0.10mM sodium phosphate-7, 50mM sodium chloride-8, 2mM DTT-9, 0.6-1.8mM E6CT-10, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 0.2-0.6mM [U-100% 13C; U-100% 15N] MAGI-1 PDZ1-11, 0.02-0.10mM sodium phosphate-12, 50mM sodium chloride-13, 2mM DTT-14, 0.6-1.8mM E6CT-15, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2LJX Structure of the monomeric N-terminal domain of HPV16 E6 oncoprotein Deposited 2011-09-30 Different construct Different mutation/modification Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 7–89（83 aa） Fragment：Zinc finger containing residues 7-89
Mutation：F47R, C80S ZN ZINC ION × 1 SOLUTION NMR
NMR measurement conditions pH 6.8；296 K；Ionic strength (raw mmCIF value) 50 mM；Pressure ambient
NMR sample composition 1 mM E6, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-100% 13C; U-100% 15N] E6, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-100% 15N] E6, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2LJY Haddock model structure of the N-terminal domain dimer of HPV16 E6 Deposited 2011-09-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 7–89（83 aa） Fragment：Zinc finger containing residues 7-86
Chain B 7–89（83 aa） Fragment：Zinc finger containing residues 7-86
Mutation：C80S Mutation：C80S ZN ZINC ION × 2 SOLUTION NMR
NMR measurement conditions pH 6.8；296 K；Ionic strength (raw mmCIF value) 50 mM NaCl；Pressure ambient
NMR sample composition 0.3 mM E6, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 0.3 mM [U-100% 15N] E6, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 0.3 mM [U-100% 13C; U-100% 15N] E6, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2LJZ Structure of the C-terminal domain of HPV16 E6 oncoprotein Deposited 2011-09-30 Different construct Different mutation/modification Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 87–158（72 aa） Fragment：Zinc finger containing residues 87-158
Mutation：C80S, C97S, C111S, C140S ZN ZINC ION × 1 SOLUTION NMR
NMR measurement conditions pH 6.8；286 K；Ionic strength (raw mmCIF value) 50 mM NaCl；Pressure ambient
NMR sample composition 1 mM E6, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-100% 13C; U-100% 15N] E6, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-100% 15N] E6, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
4GIZ Crystal structure of full-length human papillomavirus oncoprotein E6 in complex with LXXLL peptide of ubiquitin ligase E6AP at 2.55 A resolution Deposited 2012-08-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 9–150（142 aa） Fragment：unp residues 9-150
Mutation：F47R,C80S,C97S,C111S,C140S ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 9.5；290 K；10% peg8000, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K
Resolution 2.55 Å R-free 0.196
4GIZ Crystal structure of full-length human papillomavirus oncoprotein E6 in complex with LXXLL peptide of ubiquitin ligase E6AP at 2.55 A resolution Deposited 2012-08-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 9–150（142 aa） Fragment：unp residues 9-150
Mutation：F47R,C80S,C97S,C111S,C140S ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 9.5；290 K；10% peg8000, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K
Resolution 2.55 Å R-free 0.196
4JOP CFTR Associated Ligand (CAL) PDZ bound to HPV16 E6 oncoprotein C-terminal peptide (TRRETQL) Deposited 2013-03-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 152–158（7 aa） Fragment：HPV16 E6 peptide
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；32% (w/v) polyethylene glycol (PEG), 0.125 M sodium chloride, 0.1 M tris(hydroxymethyl)aminomethane (Tris), pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 1.80 Å R-free 0.219
4JOP CFTR Associated Ligand (CAL) PDZ bound to HPV16 E6 oncoprotein C-terminal peptide (TRRETQL) Deposited 2013-03-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 152–158（7 aa） Fragment：HPV16 E6 peptide
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；32% (w/v) polyethylene glycol (PEG), 0.125 M sodium chloride, 0.1 M tris(hydroxymethyl)aminomethane (Tris), pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 1.80 Å R-free 0.219
4XR8 Crystal structure of the HPV16 E6/E6AP/p53 ternary complex at 2.25 A resolution Deposited 2015-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain F 8–158（151 aa）
Mutation：C80S,C97S,C111S,C140S PEG DI(HYDROXYETHYL)ETHER × 2 ZN ZINC ION × 3 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；290 K；7.5 % PEG 20K, 0.05 M MES pH 6.5
Resolution 2.25 Å R-free 0.246
4XR8 Crystal structure of the HPV16 E6/E6AP/p53 ternary complex at 2.25 A resolution Deposited 2015-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain H 8–158（151 aa）
Mutation：C80S,C97S,C111S,C140S PEG DI(HYDROXYETHYL)ETHER × 2 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；290 K；7.5 % PEG 20K, 0.05 M MES pH 6.5
Resolution 2.25 Å R-free 0.246
6HKS Crystal structure of the PTPN3 PDZ domain bound to the HPV16 E6 oncoprotein C-terminal peptide Deposited 2018-09-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 148–158（11 aa）
Not recorded IOD IODIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；277 K；20% w/v PEG 3350 0.2 mM KI pH 7
Resolution 2.19 Å R-free 0.247
6HKS Crystal structure of the PTPN3 PDZ domain bound to the HPV16 E6 oncoprotein C-terminal peptide Deposited 2018-09-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain H 148–158（11 aa）
Not recorded IOD IODIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；277 K；20% w/v PEG 3350 0.2 mM KI pH 7
Resolution 2.19 Å R-free 0.247
6HKS Crystal structure of the PTPN3 PDZ domain bound to the HPV16 E6 oncoprotein C-terminal peptide Deposited 2018-09-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain I 148–158（11 aa）
Not recorded IOD IODIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；277 K；20% w/v PEG 3350 0.2 mM KI pH 7
Resolution 2.19 Å R-free 0.247
6HKS Crystal structure of the PTPN3 PDZ domain bound to the HPV16 E6 oncoprotein C-terminal peptide Deposited 2018-09-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain J 148–158（11 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；277 K；20% w/v PEG 3350 0.2 mM KI pH 7
Resolution 2.19 Å R-free 0.247
6HKS Crystal structure of the PTPN3 PDZ domain bound to the HPV16 E6 oncoprotein C-terminal peptide Deposited 2018-09-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain K 148–158（11 aa）
Not recorded IOD IODIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；277 K；20% w/v PEG 3350 0.2 mM KI pH 7
Resolution 2.19 Å R-free 0.247
6HKS Crystal structure of the PTPN3 PDZ domain bound to the HPV16 E6 oncoprotein C-terminal peptide Deposited 2018-09-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain L 148–158（11 aa）
Not recorded IOD IODIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；277 K；20% w/v PEG 3350 0.2 mM KI pH 7
Resolution 2.19 Å R-free 0.247
6SIV Structure of HPV16 E6 oncoprotein in complex with mutant IRF3 LxxLL motif Deposited 2019-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 6–158（153 aa）
Mutation：F1047R,C1080S,C1097S,C1111S,C1140S ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；298 K；PEG 1500 30%
Resolution 1.75 Å R-free 0.220
6SJA Structure of HPV16 E6 oncoprotein in complex with IRF3 LxxLL motif Deposited 2019-08-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 7–158（152 aa）
Mutation：F1047R,C1080S,C1097S,C1111S,C1140S ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 6.8；298 K；PEG 1500 30%
Resolution 1.50 Å R-free 0.191
6TWU MAGI1_2 complexed with a phosphomimetic 16E6 peptide Deposited 2020-01-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 149–158（10 aa）
Not recorded GOL GLYCEROL × 1 CIT CITRIC ACID × 2 CA CALCIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；22% PEG3000, 100mM Na-citrate (pH5.5), 100mM Na-citrate
Resolution 2.40 Å R-free 0.255
7UAJ Crystal structure of apo HPV16 E6 Deposited 2022-03-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 8–158（151 aa）
Chain B 8–158（151 aa）
Chain C 8–158（151 aa）
Chain D 8–158（151 aa）
Not recorded ZN ZINC ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；0.1 M HEPES, 8% ethylene glycol and 10% PEG 8000 at pH 7.5
Resolution 3.25 Å R-free 0.282
8GCR HPV16 E6-E6AP-p53 complex Deposited 2023-03-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–158（158 aa）
Mutation：C87S,C104S,C118S,C147S ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.38 Å
8JRN Structure of E6AP-E6 complex in Att1 state Deposited 2023-06-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–158（158 aa）
Chain D 1–158（158 aa）
Mutation：C87S/C104S/C118S/C147S Mutation：C87S/C104S/C118S/C147S ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
8JRO Structure of E6AP-E6 complex in Att2 state Deposited 2023-06-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–158（158 aa）
Chain D 1–158（158 aa）
Mutation：C87S/C104S/C118S/C147S Mutation：C87S/C104S/C118S/C147S ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.01 Å
8JRP Structure of E6AP-E6 complex in Att3 state Deposited 2023-06-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 9–149（141 aa）
Chain D 9–149（141 aa）
Mutation：C87S/C104S/C118S/C147S Mutation：C87S/C104S/C118S/C147S ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.58 Å
8JRQ Structure of E6AP-E6 complex in Det1 state Deposited 2023-06-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–158（158 aa）
Chain D 1–158（158 aa）
Mutation：C87S/C104S/C118S/C147S Mutation：C87S/C104S/C118S/C147S ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.15 Å
8JRR Structure of E6AP-E6 complex in Det2 state Deposited 2023-06-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 12–146（135 aa）
Chain D 12–146（135 aa）
Mutation：C87S/C104S/C118S Mutation：C87S/C104S/C118S ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.35 Å
8R1F Monomeric E6AP-E6-p53 ternary complex Deposited 2023-11-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–158（158 aa）
Mutation：C80S,C97S,C111S,C140S ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.67 Å
8R1G Dimeric ternary structure of E6AP-E6-p53 Deposited 2023-11-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–158（158 aa）
Chain E 1–158（158 aa）
Mutation：C80S,C97S,C111S,C140S Mutation：C80S,C97S,C111S,C140S ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.99 Å
9CHT Human E3 ligase E6AP in complex with HPV16-E6 and p53 Deposited 2024-07-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 8–158（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å

21 other PDB entries and 29 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name VE6_HPV16
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 4–75； UniProt 87–158

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 2fk4

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 2fk4
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2. Structure Basics 2. Structure Basics

Entry ID entry_id2fk4
Deposition date deposition_date2006-01-04
Structure title titleSolution structure of the C-terminal zinc binding domain of the HPV16 E6 oncoprotein
Keywords keywordsZINC BINDING DOMAIN, ONCOPROTEIN, Metal binding protein; Metal binding protein, Oncoprotein
Experimental Method methodSOLUTION NMR
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier11.90 Å
Radius of gyration Rg (electron density) rg_electron11.56 Å
Forward intensity I(0) i0108774000.00
Molecular weight molecular_weight80026.0 kDa
Excluded volume excluded_volume97730 ų
Envelope volume envelope_volume20024 ų
Hydration-shell volume shell_volume12179 ų
Envelope diameter envelope_diameter48.4 Å
Shell Rg shell_rg19.76 Å
Envelope Rg envelope_rg14.57 Å
Shape Rg shape_rg11.50 Å
Total Rg total_rg12.12 Å
Total atoms total_atoms11070
Residues n_residues670
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax43.3 Å
Rg (real space) rg_real11.86 Å
Rg uncertainty (real space) rg_real_error0.29 Å
I(0) (real space) i0_real1.0880e+08
I(0) uncertainty (real space) i0_real_error1.1530e+06
Rg (reciprocal space) rg_reciprocal11.86 Å
I(0) (reciprocal space) i0_reciprocal108800000.0000
Solution quality estimate total_estimate0.6435
Solution quality rating solution_quality REASONABLE a REASONABLE solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary15.9 Å
Skewness Skewness skewness0.248
Kurtosis Kurtosis kurtosis0.071
Angular range angular_range— – 0.5000 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha111200.0000
Real-space data points n_real_points80
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.545; Stabil: 1.000; Sysdev: 0.256; Positv: 1.000; Valcen: 0.982; Smooth: 0.974
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (2)

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7. Fold Classification (SCOP + CATH) 3 domains

SCOP 2.08 (2 domains)

Domain ID domain_idd2fk4a1
Class classg — Small proteins
Fold Fold foldg.90 — E6 C-terminal domain-like
Superfamily Superfamily superfamilyg.90.1 — E6 C-terminal domain-like
Family Family familyg.90.1.1 — E6 C-terminal domain-like
Domain ID domain_idd2fk4a2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags

CATH v4.4 (1 domains)

Domain ID domain_id2fk4A00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology240 — CRO Repressor
Homologous superfamily homologous superfamily40 — E6 early regulatory protein
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8. Citations (2)

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9. Files and Curves (10)