2gum

Crystal structure of the extracellular domain of glycoprotein B from Herpes Simplex Virus type I

Method: X-RAY DIFFRACTION Dmax: 160.7 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 2gum

P(r) 距离分布 P(r) Distribution

P(r) distribution for 2gum
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id2gum
沉积日期 deposition_date2006-05-01
结构标题 titleCrystal structure of the extracellular domain of glycoprotein B from Herpes Simplex Virus type I
关键词 keywordsENVELOPE GLYCOPROTEIN, MEMBRANE FUSION, VIRAL PROTEIN; VIRAL PROTEIN
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier50.56
回转半径 Rg (电子) rg_electron50.93
零角强度 I(0) i0599537000.00
分子量 molecular_weight198520.0 kDa
排除体积 excluded_volume246500 ų
包络体积 envelope_volume352540 ų
水化壳体积 shell_volume62296 ų
包络直径 envelope_diameter172.0
壳层 Rg shell_rg48.23
包络 Rg envelope_rg50.33
形状 Rg shape_rg50.91
总 Rg total_rg50.86
总原子数 total_atoms14000
残基数 n_residues1732
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax160.7
Rg (实空间) rg_real50.98
Rg 误差 (实空间) rg_real_error1.49
I(0) (实空间) i0_real5.9950e+08
I(0) 误差 (实空间) i0_real_error1.1290e+07
Rg (倒空间) rg_reciprocal50.20
I(0) (倒空间) i0_reciprocal598900000.0000
解质量估计 total_estimate0.7752
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks3
主峰位置 r_peak_primary41.5
偏度 Skewness skewness0.552
峰度 Kurtosis kurtosis-0.530
角度范围 angular_range— – 0.1550 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha56570000.0000
实空间数据点数 n_real_points32
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.752; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.812; Smooth: 0.007

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (3)

6. 折叠分类 (SCOP + CATH) 15 domains

SCOP 2.08 (3 domains)

结构域编号 domain_idd2guma1
类 Class classe — Multi-domain proteins (alpha and beta)
折叠类型 Fold folde.76 — Viral glycoprotein ectodomain-like
超家族 Superfamily superfamilye.76.1 — Viral glycoprotein ectodomain-like
家族 Family familye.76.1.1 — Glycoprotein B-like
结构域编号 domain_idd2gumb_
类 Class classe — Multi-domain proteins (alpha and beta)
折叠类型 Fold folde.76 — Viral glycoprotein ectodomain-like
超家族 Superfamily superfamilye.76.1 — Viral glycoprotein ectodomain-like
家族 Family familye.76.1.1 — Glycoprotein B-like
结构域编号 domain_idd2gumc_
类 Class classe — Multi-domain proteins (alpha and beta)
折叠类型 Fold folde.76 — Viral glycoprotein ectodomain-like
超家族 Superfamily superfamilye.76.1 — Viral glycoprotein ectodomain-like
家族 Family familye.76.1.1 — Glycoprotein B-like

CATH v4.4 (12 domains)

结构域编号 domain_id2gumA01
类 Class class2 — Mainly Beta
架构 Architecture architecture30 — Roll
拓扑 Topology topology30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily1230
结构域编号 domain_id2gumA02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily1890
结构域编号 domain_id2gumA03
类 Class class2 — Mainly Beta
架构 Architecture architecture30 — Roll
拓扑 Topology topology29 — PH-domain like
同源超家族 H-superfamily homologous superfamily100
结构域编号 domain_id2gumA05
类 Class class6 — Special
架构 Architecture architecture10 — Helix non-globular
拓扑 Topology topology250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily3280
结构域编号 domain_id2gumB01
类 Class class2 — Mainly Beta
架构 Architecture architecture30 — Roll
拓扑 Topology topology30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily1230
结构域编号 domain_id2gumB02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily1890
结构域编号 domain_id2gumB03
类 Class class2 — Mainly Beta
架构 Architecture architecture30 — Roll
拓扑 Topology topology29 — PH-domain like
同源超家族 H-superfamily homologous superfamily100
结构域编号 domain_id2gumB05
类 Class class6 — Special
架构 Architecture architecture10 — Helix non-globular
拓扑 Topology topology250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily3280
结构域编号 domain_id2gumC01
类 Class class2 — Mainly Beta
架构 Architecture architecture30 — Roll
拓扑 Topology topology30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily1230
结构域编号 domain_id2gumC02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily1890
结构域编号 domain_id2gumC03
类 Class class2 — Mainly Beta
架构 Architecture architecture30 — Roll
拓扑 Topology topology29 — PH-domain like
同源超家族 H-superfamily homologous superfamily100
结构域编号 domain_id2gumC05
类 Class class6 — Special
架构 Architecture architecture10 — Helix non-globular
拓扑 Topology topology250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily3280

7. 引用文献 (1)

8. 文件与曲线 (10)