2hpo

Structure of Aminopeptidase N from E. coli Suggests a Compartmentalized, Gated Active Site

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Aminopeptidase N

Escherichia coli K12

UniProt P04825

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein monomer Monomer Protein 1 ZINC ION × 1 GLYCEROL × 2 water × 1 Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name AMPN_ECOLI
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A; PDBConstruct 23–891; UniProt 1–869

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id2hpo
Deposition date deposition_date2006-07-17
Structure title titleStructure of Aminopeptidase N from E. coli Suggests a Compartmentalized, Gated Active Site
Keywords keywordsmultidomain, closed, compartmentalized active site, HYDROLASE; HYDROLASE
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2hpo__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2hpo__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

2hpo__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg(Guinier)28.92 Å
Rg (electron density)28.05 Å
Total Rg28.74 Å
Atom count6953
Residues866
Excluded volume123110 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 2hpo__assembly_1__model_1 monomeric (1) Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (4)

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6. Fold Classification (SCOP + CATH) 5 domains

CATH v4.4 (5 domains)

Domain ID domain_id2hpoA01
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology40 — Immunoglobulin-like
Homologous superfamily homologous superfamily1730 — tricorn interacting facor f3 domain
Domain ID domain_id2hpoA02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology2010 — Zincin-like
Homologous superfamily homologous superfamily30 —
Domain ID domain_id2hpoA03
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology390 — Neutral Protease; domain 2
Homologous superfamily homologous superfamily10 — Neutral Protease Domain 2
Domain ID domain_id2hpoA04
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology40 — Immunoglobulin-like
Homologous superfamily homologous superfamily1840 — Aminopeptidase N, middle-beta domain
Domain ID domain_id2hpoA05
Class class1 — Mainly Alpha
Architecture architecture25 — Alpha Horseshoe
Topology topology50 — Zincin-like fold
Homologous superfamily homologous superfamily10 — Peptidase M1, alanyl aminopeptidase, C-terminal domain
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7. Citations (1)