2j6h

E. coli glucosamine-6-P synthase in complex with glucose-6P and 5-oxo- L-norleucine

Method: X-RAY DIFFRACTION Dmax: 113.6 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 2j6h

P(r) 距离分布 P(r) Distribution

P(r) distribution for 2j6h
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id2j6h
沉积日期 deposition_date2006-09-28
结构标题 titleE. coli glucosamine-6-P synthase in complex with glucose-6P and 5-oxo- L-norleucine
关键词 keywordsTRANSFERASE, AMMONIA CHANNELING; TRANSFERASE
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier33.26
回转半径 Rg (电子) rg_electron32.45
零角强度 I(0) i0281117000.00
分子量 molecular_weight134230.0 kDa
排除体积 excluded_volume167790 ų
包络体积 envelope_volume198720 ų
水化壳体积 shell_volume50288 ų
包络直径 envelope_diameter121.8
壳层 Rg shell_rg39.86
包络 Rg envelope_rg32.77
形状 Rg shape_rg32.46
总 Rg total_rg32.93
总原子数 total_atoms9441
残基数 n_residues1216
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax113.6
Rg (实空间) rg_real33.32
Rg 误差 (实空间) rg_real_error0.86
I(0) (实空间) i0_real2.8110e+08
I(0) 误差 (实空间) i0_real_error4.3980e+06
Rg (倒空间) rg_reciprocal33.30
I(0) (倒空间) i0_reciprocal281100000.0000
解质量估计 total_estimate0.7803
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks4
主峰位置 r_peak_primary40.2
偏度 Skewness skewness0.490
峰度 Kurtosis kurtosis0.024
角度范围 angular_range— – 0.2400 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha183300000.0000
实空间数据点数 n_real_points49
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.726; Stabil: 0.991; Sysdev: 1.000; Positv: 1.000; Valcen: 0.988; Smooth: 0.000

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (4)

6. 折叠分类 (SCOP + CATH) 10 domains

SCOP 2.08 (4 domains)

结构域编号 domain_idd2j6ha1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.80 — SIS domain
超家族 Superfamily superfamilyc.80.1 — SIS domain
家族 Family familyc.80.1.1 — double-SIS domain
结构域编号 domain_idd2j6ha2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.153 — Ntn hydrolase-like
超家族 Superfamily superfamilyd.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
家族 Family familyd.153.1.0 — automated matches
结构域编号 domain_idd2j6hb1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.80 — SIS domain
超家族 Superfamily superfamilyc.80.1 — SIS domain
家族 Family familyc.80.1.1 — double-SIS domain
结构域编号 domain_idd2j6hb2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.153 — Ntn hydrolase-like
超家族 Superfamily superfamilyd.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
家族 Family familyd.153.1.0 — automated matches

CATH v4.4 (6 domains)

结构域编号 domain_id2j6hA01
类 Class class3 — Alpha Beta
架构 Architecture architecture60 — 4-Layer Sandwich
拓扑 Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
同源超家族 H-superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
结构域编号 domain_id2j6hA02
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily10490 — Glucose-6-phosphate isomerase like protein; domain 1
结构域编号 domain_id2j6hA03
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily10490 — Glucose-6-phosphate isomerase like protein; domain 1
结构域编号 domain_id2j6hB01
类 Class class3 — Alpha Beta
架构 Architecture architecture60 — 4-Layer Sandwich
拓扑 Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
同源超家族 H-superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
结构域编号 domain_id2j6hB02
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily10490 — Glucose-6-phosphate isomerase like protein; domain 1
结构域编号 domain_id2j6hB03
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily10490 — Glucose-6-phosphate isomerase like protein; domain 1

7. 引用文献 (2)

8. 文件与曲线 (10)