← Back to search
2rhk

Crystal structure of influenza A NS1A protein in complex with F2F3 fragment of human cellular factor CPSF30, Northeast Structural Genomics Targets OR8C and HR6309A

Method: X-RAY DIFFRACTION Dmax: 89.8 Å Quality: GOOD
▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

Non-structural protein 1

Influenza A Virus

UniProt P03495

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count Chain A； UniProt 85–215 Chain B； UniProt 85–215 Fragment：NS1A effector domain (UNP residues 85-215) Cleavage and polyadenylation specificity factor subunit 4 × 2 （O95639） NO3 NITRATE ION × 5 ZN ZINC ION × 4 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 X-RAY DIFFRACTION X-ray crystallization conditions：pH 5.5；293 K；0.5 M KNO3, 10% sucroes, 0.1M sodium acitate, pH 5.5, EVAPORATION, temperature 293K, pH 5.50 Resolution 1.95 Å R-free 0.234
2 Protein heterocomplex Heteromer Protein × 6 PDB declaration: hexameric（6） Consistent with protein copy count Chain A； UniProt 85–215 Chain B； UniProt 85–215 Fragment：NS1A effector domain (UNP residues 85-215) Cleavage and polyadenylation specificity factor subunit 4 × 3 （O95639） NO3 NITRATE ION × 9 ZN ZINC ION × 6 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 X-RAY DIFFRACTION X-ray crystallization conditions：pH 5.5；293 K；0.5 M KNO3, 10% sucroes, 0.1M sodium acitate, pH 5.5, EVAPORATION, temperature 293K, pH 5.50 Resolution 1.95 Å R-free 0.234
3 Protein heterocomplex Heteromer Protein × 3 PDB declaration: trimeric（3） Consistent with protein copy count Chain A； UniProt 85–215 Fragment：NS1A effector domain (UNP residues 85-215) Cleavage and polyadenylation specificity factor subunit 4 × 2 （O95639） NO3 NITRATE ION × 5 ZN ZINC ION × 4 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 X-RAY DIFFRACTION X-ray crystallization conditions：pH 5.5；293 K；0.5 M KNO3, 10% sucroes, 0.1M sodium acitate, pH 5.5, EVAPORATION, temperature 293K, pH 5.50 Resolution 1.95 Å R-free 0.234
4 Protein heterocomplex Heteromer Protein × 3 PDB declaration: trimeric（3） Consistent with protein copy count Chain A； UniProt 85–215 Chain B； UniProt 85–215 Fragment：NS1A effector domain (UNP residues 85-215) Cleavage and polyadenylation specificity factor subunit 4 × 1 （O95639） NO3 NITRATE ION × 4 ZN ZINC ION × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 X-RAY DIFFRACTION X-ray crystallization conditions：pH 5.5；293 K；0.5 M KNO3, 10% sucroes, 0.1M sodium acitate, pH 5.5, EVAPORATION, temperature 293K, pH 5.50 Resolution 1.95 Å R-free 0.234

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 2RHK Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1AIL N-TERMINAL FRAGMENT OF NS1 PROTEIN FROM INFLUENZA A VIRUS Deposited 1997-04-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–72（72 aa） Fragment：RNA-BINDING DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；NATIVE CRYSTALS WERE GROWN FROM 50MM NAH2PO4, 100MM NACL, 1MM NAN3 AT PH 6.5; MERCURY DERIVATIVE CRYSTAL WAS PREPARED BY SOAKING THE NATIVE CRYSTAL IN 2MM CH3HGCL WITH 10% PEG 6000 AT PH 8.0; PLATINUM DERIVATIVE CRYSTAL WAS PREPARED BY SOAKING THE NATIVE CRYSTAL IN 4MM PT(NH3)2CL2 WITH 10% PEG 6000 AT PH 6.5.
Resolution 1.90 Å R-free 0.229
1NS1 RNA-BINDING DOMAIN OF NON-STRUCTURAL PROTEIN 1 FROM INFLUENZA VIRUS, NMR, 16 STRUCTURES Deposited 1997-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–73（73 aa） Fragment：RNA-BINDING DOMAIN, RESIDUES 1 - 73
Chain B 1–73（73 aa） Fragment：RNA-BINDING DOMAIN, RESIDUES 1 - 73
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6；293 K
Resolution not provided
3EE8 Structure of NS1 effector domain Deposited 2008-09-04 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 84–205（122 aa） Fragment：Effector domain (UNP residues 84-205)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；298 K；12% (w/v) PEG3350, and 4% (v/v) Tacsimate (pH6.0), VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.60 Å R-free 0.284
3EE8 Structure of NS1 effector domain Deposited 2008-09-04 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 84–205（122 aa） Fragment：Effector domain (UNP residues 84-205)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；298 K；12% (w/v) PEG3350, and 4% (v/v) Tacsimate (pH6.0), VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.60 Å R-free 0.284
3EE9 Structure of NS1 effector domain Deposited 2008-09-04 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 84–205（122 aa） Fragment：Effector domain (UNP residues 84-205)
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions Batch；pH 5.5；298 K；20% (w/v) PEG400, 0.1M CH3COONa (pH5.5), 0.1M MgSO4, Batch, temperature 298K
Resolution 2.14 Å R-free 0.234
3EE9 Structure of NS1 effector domain Deposited 2008-09-04 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 84–205（122 aa） Fragment：Effector domain (UNP residues 84-205)
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions Batch；pH 5.5；298 K；20% (w/v) PEG400, 0.1M CH3COONa (pH5.5), 0.1M MgSO4, Batch, temperature 298K
Resolution 2.14 Å R-free 0.234
3KWG X-ray structure of NS1 effector domain W187A mutant Deposited 2009-12-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 78–205（128 aa） Fragment：NS1 effector domain
Mutation：W187A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 5.5；277 K；0.3M magnesium formate, 0.1M Bis-Tris , pH 5.5, EVAPORATION, temperature 277K
Resolution 2.21 Å R-free 0.233
3KWG X-ray structure of NS1 effector domain W187A mutant Deposited 2009-12-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 78–205（128 aa） Fragment：NS1 effector domain
Mutation：W187A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 5.5；277 K；0.3M magnesium formate, 0.1M Bis-Tris , pH 5.5, EVAPORATION, temperature 277K
Resolution 2.21 Å R-free 0.233
3KWG X-ray structure of NS1 effector domain W187A mutant Deposited 2009-12-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 78–205（128 aa） Fragment：NS1 effector domain
Chain B 78–205（128 aa） Fragment：NS1 effector domain
Mutation：W187A Mutation：W187A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 5.5；277 K；0.3M magnesium formate, 0.1M Bis-Tris , pH 5.5, EVAPORATION, temperature 277K
Resolution 2.21 Å R-free 0.233
3KWI X-ray structure of NS1 effector domain W187Y mutant Deposited 2009-12-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 78–205（128 aa） Fragment：NS1 effector domain
Mutation：W187Y No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 5.5；277 K；0.3M magnesium formate and 0.1M Bis-Tris , pH 5.5, EVAPORATION, temperature 277K
Resolution 2.21 Å R-free 0.258
3KWI X-ray structure of NS1 effector domain W187Y mutant Deposited 2009-12-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 78–205（128 aa） Fragment：NS1 effector domain
Mutation：W187Y No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 5.5；277 K；0.3M magnesium formate and 0.1M Bis-Tris , pH 5.5, EVAPORATION, temperature 277K
Resolution 2.21 Å R-free 0.258

6 other PDB entries and 11 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NS1_IAUDO
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 2–132； UniProt 85–215 Author chain B； PDBConstruct 2–132； UniProt 85–215

Cleavage and polyadenylation specificity factor subunit 4

Homo sapiens

UniProt O95639

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count Chain C； UniProt 61–121 Chain D； UniProt 61–121 Fragment：F2F3 Zinc-binding domains (UNP residues 61-121) Mutation：P94S Non-structural protein 1 × 2 （P03495） NO3 NITRATE ION × 5 ZN ZINC ION × 4 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 X-RAY DIFFRACTION X-ray crystallization conditions：pH 5.5；293 K；0.5 M KNO3, 10% sucroes, 0.1M sodium acitate, pH 5.5, EVAPORATION, temperature 293K, pH 5.50 Resolution 1.95 Å R-free 0.234
2 Protein heterocomplex Heteromer Protein × 6 PDB declaration: hexameric（6） Consistent with protein copy count Chain C； UniProt 61–121 Chain D； UniProt 61–121 Fragment：F2F3 Zinc-binding domains (UNP residues 61-121) Mutation：P94S Non-structural protein 1 × 3 （P03495） NO3 NITRATE ION × 9 ZN ZINC ION × 6 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 X-RAY DIFFRACTION X-ray crystallization conditions：pH 5.5；293 K；0.5 M KNO3, 10% sucroes, 0.1M sodium acitate, pH 5.5, EVAPORATION, temperature 293K, pH 5.50 Resolution 1.95 Å R-free 0.234
3 Protein heterocomplex Heteromer Protein × 3 PDB declaration: trimeric（3） Consistent with protein copy count Chain C； UniProt 61–121 Chain D； UniProt 61–121 Fragment：F2F3 Zinc-binding domains (UNP residues 61-121) Mutation：P94S Non-structural protein 1 × 1 （P03495） NO3 NITRATE ION × 5 ZN ZINC ION × 4 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 X-RAY DIFFRACTION X-ray crystallization conditions：pH 5.5；293 K；0.5 M KNO3, 10% sucroes, 0.1M sodium acitate, pH 5.5, EVAPORATION, temperature 293K, pH 5.50 Resolution 1.95 Å R-free 0.234
4 Protein heterocomplex Heteromer Protein × 3 PDB declaration: trimeric（3） Consistent with protein copy count Chain D； UniProt 61–121 Fragment：F2F3 Zinc-binding domains (UNP residues 61-121) Mutation：P94S Non-structural protein 1 × 2 （P03495） NO3 NITRATE ION × 4 ZN ZINC ION × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 X-RAY DIFFRACTION X-ray crystallization conditions：pH 5.5；293 K；0.5 M KNO3, 10% sucroes, 0.1M sodium acitate, pH 5.5, EVAPORATION, temperature 293K, pH 5.50 Resolution 1.95 Å R-free 0.234

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 2RHK Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2D9N Solution structure of CCCH type zinc-finger domain 2 in Cleavage and polyadenylation specificity factor Deposited 2005-12-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 61–126（66 aa） Fragment：CCCH-type zinc finger motif
Not recorded ZN ZINC ION × 2 SOLUTION NMR
NMR measurement conditions pH 7；298 K；Ionic strength (raw mmCIF value) 100mM；Pressure ambient
NMR sample composition 20mM d-Tris-HCl(pH7.0), 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 50uM ZnCl2+1mM IDA, 90% H2O, 10% D2O | 90% H2O/10% D2O
Resolution not provided
6DNH Cryo-EM structure of human CPSF-160-WDR33-CPSF-30-PAS RNA complex at 3.4 A resolution Deposited 2018-06-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 3 PDB declaration: tetrameric
Chain C 1–244（244 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；25 mM Tris-HCl, pH 7.9, 380 mM NaCl, 5 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6FUW Cryo-EM structure of the human CPSF160-WDR33-CPSF30 complex bound to the PAS AAUAAA motif at 3.1 Angstrom resolution Deposited 2018-02-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 3 PDB declaration: tetrameric
Chain C 1–178（178 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；15 seconds wait time prior to blotting
Resolution 3.07 Å
6URG Cryo-EM structure of human CPSF160-WDR33-CPSF30-CPSF100 PIM complex Deposited 2019-10-23 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain C 1–244（244 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 5 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6URO Cryo-EM structure of human CPSF160-WDR33-CPSF30-PAS RNA-CstF77 complex Deposited 2019-10-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 5 PDB declaration: hexameric
Chain C 1–244（244 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 5 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7ZYH Crystal structure of human CPSF30 in complex with hFip1 Deposited 2022-05-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 118–178（61 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；292.15 K；1.626 M (NH4)SO4, 0.1 M Bis-Tris pH 6.5
Resolution 2.20 Å R-free 0.264
7ZYH Crystal structure of human CPSF30 in complex with hFip1 Deposited 2022-05-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain D 118–178（61 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；292.15 K；1.626 M (NH4)SO4, 0.1 M Bis-Tris pH 6.5
Resolution 2.20 Å R-free 0.264
7ZYH Crystal structure of human CPSF30 in complex with hFip1 Deposited 2022-05-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain G 118–178（61 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；292.15 K；1.626 M (NH4)SO4, 0.1 M Bis-Tris pH 6.5
Resolution 2.20 Å R-free 0.264
7ZYH Crystal structure of human CPSF30 in complex with hFip1 Deposited 2022-05-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain J 118–178（61 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；292.15 K；1.626 M (NH4)SO4, 0.1 M Bis-Tris pH 6.5
Resolution 2.20 Å R-free 0.264
8E3I CRYO-EM STRUCTURE OF the human MPSF IN COMPLEX WITH THE AUUAAA poly(A) signal Deposited 2022-08-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 3 PDB declaration: tetrameric
Chain C 1–244（244 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；25 mM Tris (pH 8.0), 150 mM NaCl, and 5 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.53 Å
8E3Q CRYO-EM STRUCTURE OF the human MPSF Deposited 2022-08-17 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1–244（244 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；25 mM Tris (pH 8.0), 150 mM NaCl, and 5 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.68 Å
8R8R Cryo-EM structure of the human mPSF with PAPOA C-terminus peptide (PAPOAc) Deposited 2023-11-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: pentameric
Chain C 2–244（243 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.79 Å
9OXE CRYO-EM STRUCTURE OF the human mPSF IN COMPLEX WITH THE AGUAAA poly(A) signal Deposited 2025-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 3 PDB declaration: tetrameric
Chain C 1–244（244 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.53 Å
9OXS CRYO-EM STRUCTURE OF the human mPSF IN COMPLEX WITH THE AAUAAU poly(A) signal Deposited 2025-06-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 3 PDB declaration: tetrameric
Chain C 1–244（244 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.07 Å

11 other PDB entries and 14 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CPSF4_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain C； PDBConstruct 12–72； UniProt 61–121 Author chain D； PDBConstruct 12–72； UniProt 61–121

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 2rhk

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 2rhk
Download Download
▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id2rhk
Deposition date deposition_date2007-10-09
Structure title titleCrystal structure of influenza A NS1A protein in complex with F2F3 fragment of human cellular factor CPSF30, Northeast Structural Genomics Targets OR8C and HR6309A
Keywords keywords;Influenza A, Nonstructural protein, viral protein: host complex, Zn finger, Alternative splicing, Cytoplasm, Host-virus interaction, Interferon antiviral system evasion, Nucleus, RNA-binding, Suppressor of RNA silencing, Metal-binding, mRNA processing, Zinc, Zinc-finger, METAL BINDING PROTEIN, VIRAL PROTEIN-METAL BINDING PROTEIN COMPLEX, VIRAL PROTEIN-NUCLEAR PROTEIN COMPLEX, Structural Genomics, PSI-2, Protein Structure Initiative, Northeast Structural Genomics Consortium, NESG ;; VIRAL PROTEIN/NUCLEAR PROTEIN
Experimental Method methodX-RAY DIFFRACTION
▼

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier24.66 Å
Radius of gyration Rg (electron density) rg_electron24.02 Å
Forward intensity I(0) i031223300.00
Molecular weight molecular_weight42260.0 kDa
Excluded volume excluded_volume52630 ų
Envelope volume envelope_volume65865 ų
Hydration-shell volume shell_volume23798 ų
Envelope diameter envelope_diameter92.6 Å
Shell Rg shell_rg30.07 Å
Envelope Rg envelope_rg24.83 Å
Shape Rg shape_rg24.09 Å
Total Rg total_rg24.54 Å
Total atoms total_atoms2943
Residues n_residues361
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
▼

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax89.8 Å
Rg (real space) rg_real24.71 Å
Rg uncertainty (real space) rg_real_error0.84 Å
I(0) (real space) i0_real3.1220e+07
I(0) uncertainty (real space) i0_real_error4.3610e+05
Rg (reciprocal space) rg_reciprocal24.70 Å
I(0) (reciprocal space) i0_reciprocal31220000.0000
Solution quality estimate total_estimate0.8406
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary27.1 Å
Skewness Skewness skewness0.422
Kurtosis Kurtosis kurtosis-0.138
Angular range angular_range— – 0.3200 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha5522000.0000
Real-space data points n_real_points64
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.703; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.825; Smooth: 0.989
▶

5. Crystallography and Experiment 5. Crystallography & Experiment

▶

6. Entities and Polymers Entities & Polymers (6)

▼

7. Fold Classification (SCOP + CATH) 6 domains

SCOP 2.08 (2 domains)

Domain ID domain_idd2rhka_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.299 — Ns1 effector domain-like
Superfamily Superfamily superfamilyd.299.1 — Ns1 effector domain-like
Family Family familyd.299.1.1 — Ns1 effector domain-like
Domain ID domain_idd2rhkb_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.299 — Ns1 effector domain-like
Superfamily Superfamily superfamilyd.299.1 — Ns1 effector domain-like
Family Family familyd.299.1.1 — Ns1 effector domain-like

CATH v4.4 (4 domains)

Domain ID domain_id2rhkA00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology420 — Nucleotidyltransferase; domain 5
Homologous superfamily homologous superfamily330 — Influenza virus non-structural protein, effector domain
Domain ID domain_id2rhkB00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology420 — Nucleotidyltransferase; domain 5
Homologous superfamily homologous superfamily330 — Influenza virus non-structural protein, effector domain
Domain ID domain_id2rhkC00
Class class4 — Few Secondary Structures
Architecture architecture10 — Irregular
Topology topology1000 — CCCH zinc finger
Homologous superfamily homologous superfamily10 — Zinc finger, CCCH-type
Domain ID domain_id2rhkD00
Class class4 — Few Secondary Structures
Architecture architecture10 — Irregular
Topology topology1000 — CCCH zinc finger
Homologous superfamily homologous superfamily10 — Zinc finger, CCCH-type
▶

8. Citations (1)

▶

9. Files and Curves (10)