TETRACYCLINE REPRESSOR
Escherichia coli
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein homooligomer Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count | Chain A； UniProt 1–207 | Not recorded | MG MAGNESIUM ION × 2 CTC 7-CHLOROTETRACYCLINE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions | Resolution 2.10 Å |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 2TCT | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1A6I TET REPRESSOR, CLASS D VARIANT Deposited 1998-02-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–217（216 aa）
|
Mutation：A2S, N5D, R6K, E7S, S8K, D11N, A12S, T20V, D23E, I36V | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；pH 8.0
|
Resolution 2.40 Å R-free 0.275 |
| 1BJZ TETRACYCLINE CHELATED MG2+-ION INITIATES HELIX UNWINDING FOR TET REPRESSOR INDUCTION Deposited 1998-06-29 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–207（206 aa）
|
Mutation：A2S | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；pH 8.0
|
Resolution 2.20 Å R-free 0.296 |
| 1ORK TET REPRESSOR, CLASS D IN COMPLEX WITH 9-(N,N-DIMETHYLGLYCYLAMIDO)-6-DEMETHYL-6-DEOXY-TETRACYCLINE Deposited 1998-05-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–207（206 aa）
|
Mutation：S2A | MG MAGNESIUM ION × 2 ATC 9-(N,N-DIMETHYLGLYCYLAMIDO)-6-DEOXY-6-DEMETHYL-TETRACYCLINE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；pH 8.0
|
Resolution 2.40 Å R-free 0.275 |
| 1QPI CRYSTAL STRUCTURE OF TETRACYCLINE REPRESSOR/OPERATOR COMPLEX Deposited 1999-05-25 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
3–205（203 aa）
|
Not recorded | IMD IMIDAZOLE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；pH 8.00
|
Resolution 2.50 Å R-free 0.307 |
| 2FJ1 Crystal Structure Analysis of Tet Repressor (class D) in Complex with 7-Chlortetracycline-Nickel(II) Deposited 2005-12-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–208（207 aa）
Fragment：residues 2-208
|
Mutation：A2S | NI NICKEL (II) ION × 2 CL CHLORIDE ION × 6 CTC 7-CHLOROTETRACYCLINE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；1.58 M Ammonium sulphate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 2.20 Å R-free 0.215 |
| 2NS7 How an in vitro selected peptide mimics the antibiotic tetracycline to induce TET repressor Deposited 2006-11-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
188–208（21 aa）
Chain B
188–208（21 aa）
|
Mutation：C68S, C88N, C121T, C144S Mutation：C68S, C88N, C121T, C144S | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；292 K；1.4M ammonium sulfate, 0.1M sodium chloride, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 2.40 Å R-free 0.277 |
| 2NS7 How an in vitro selected peptide mimics the antibiotic tetracycline to induce TET repressor Deposited 2006-11-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain C
188–208（21 aa）
Chain D
188–208（21 aa）
|
Mutation：C68S, C88N, C121T, C144S Mutation：C68S, C88N, C121T, C144S | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；292 K；1.4M ammonium sulfate, 0.1M sodium chloride, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 2.40 Å R-free 0.277 |
| 2NS8 How an in vitro selected peptide mimics the antibiotic tetracycline to induce TET repressor Deposited 2006-11-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric |
Chain A
188–208（21 aa）
Chain B
188–208（21 aa）
|
Mutation：C68S, C88N, C121T, C144S Mutation：C68S, C88N, C121T, C144S | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.8；292 K；4M sodium formate, 0.1M Tris-HCl, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 2.55 Å R-free 0.270 |
| 2NS8 How an in vitro selected peptide mimics the antibiotic tetracycline to induce TET repressor Deposited 2006-11-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
188–208（21 aa）
Chain D
188–208（21 aa）
|
Mutation：C68S, C88N, C121T, C144S Mutation：C68S, C88N, C121T, C144S | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.8；292 K；4M sodium formate, 0.1M Tris-HCl, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 2.55 Å R-free 0.270 |
| 2NS8 How an in vitro selected peptide mimics the antibiotic tetracycline to induce TET repressor Deposited 2006-11-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Insufficient information Heteromer；Protein × 9 PDB declaration: nonameric |
Chain A
188–208（21 aa）
Chain B
188–208（21 aa）
Chain C
188–208（21 aa）
Chain D
188–208（21 aa）
|
Mutation：C68S, C88N, C121T, C144S Mutation：C68S, C88N, C121T, C144S Mutation：C68S, C88N, C121T, C144S Mutation：C68S, C88N, C121T, C144S | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.8；292 K；4M sodium formate, 0.1M Tris-HCl, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 2.55 Å R-free 0.270 |
| 2O7O Crystal structure analysis of TetR(D) complex with doxycycline Deposited 2006-12-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–207（207 aa）
Fragment：residues 2-208
|
Mutation：A2S | MG MAGNESIUM ION × 2 SO4 SULFATE ION × 2 CL CHLORIDE ION × 4 DXT (4S,4AR,5S,5AR,6R,12AS)-4-(DIMETHYLAMINO)-3,5,10,12,12A-PENTAHYDROXY-6-METHYL-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2-CARBOXAMIDE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；1.1M AMMONIUM SULPHATE, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.89 Å R-free 0.243 |
| 2TRT TETRACYCLINE REPRESSOR CLASS D Deposited 1994-03-04 | Different construct Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–217（217 aa）
|
Not recorded | MG MAGNESIUM ION × 2 TAC TETRACYCLINE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.50 Å |
| 2VKE Tet repressor class D complexed with cobalt and tetracycline Deposited 2007-12-18 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–208（207 aa）
Fragment：RESIDUES 2-208
|
Not recorded | TAC TETRACYCLINE × 2 CO COBALT (II) ION × 2 CL CHLORIDE ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；HANGING DROP VAPOR DIFFUSION WITH 2 UL (18 MG/ML PROTEIN) AND 2 UL (0.8 M AS, 50 MM TRIS/CL PH 7.5, 200 MM NACL, 5 MM COCL2). CRYSTAL WAS CRYOCOOLED WITH PARAFFIN OIL AS CRYOPROTECTANT. CRYSTAL SIZE WAS 0.6 X 0.6 X 0.2 MM
|
Resolution 1.62 Å R-free 0.297 |
| 2VKV TetR (BD) variant L17G with reverse phenotype Deposited 2008-01-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
51–208（158 aa）
Fragment：RESIDUES 1-50,51-208
|
Mutation：YES | TDC 5A,6-ANHYDROTETRACYCLINE × 2 MG MAGNESIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.7；277 K；0.1 M TRIS-HCL (PH 8.7), 0.3 M MGCL2, 20 % PEG 4000, 3 % DIOXANE, AT 277 K
|
Resolution 1.74 Å R-free 0.225 |
| 2X6O Tet Repressor class D in complex with 7-chlor-2-cyano-iso- tetracycline Deposited 2010-02-18 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–208（206 aa）
Fragment：DNA-BINDING DOMAIN, RESIDUES 3-208
|
Not recorded | 2TC (4S,4AS,6S,8AS)-6-[(1S)-7-CHLORO-4-HYDROXY-1-METHYL-3-OXO-1,3-DIHYDRO-2-BENZOFURAN-1-YL]-4-(DIMETHYLAMINO)-3,8A-DIHYDROXY-1,8-DIOXO-1,4,4A,5,6,7,8,8A-OCTAHYDRONAPHTHALENE-2-CARBONITRILE × 2 CL CHLORIDE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；50 MM TRIS/HCL PH 9.5, 750 MM (NH4)2SO4, 70 MM NACL, 1 MM EDTA
|
Resolution 2.30 Å R-free 0.269 |
| 2X9D Tet repressor (class D) in complex with iso-7-chlortetracycline Deposited 2010-03-15 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–208（207 aa）
Fragment：RESIDUES 2-208
|
Mutation：YES | ITC ISO-7-CHLORTETRACYCLINE × 2 CL CHLORIDE ION × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；0.002ML PROTEIN/COMPLEX (0.050 ML TETR(D) (C = 17.3 MG/ML)50 ML 2MM ISO-CLTC) PLUS ML BUFFER (50 MM TRIS PH 8, 1.1 M (NH4)2SO4, 100 MM NACL, 10 MM MGCL2) HANGING DROP AGAINST 0.5 ML BUFFER
|
Resolution 2.34 Å R-free 0.280 |
| 2XB5 Tet repressor (class D) in complex with 7-Iodotetracycline Deposited 2010-04-05 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–208（207 aa）
Fragment：RESIDUES 2-208
|
Not recorded | I7T 7-IODOTETRACYCLINE × 2 MG MAGNESIUM ION × 2 CL CHLORIDE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；10MICROL PROTEIN (0.2MM TETR, 1MM IODOTC, 5MM MGCL2), 5MICROL RESERVOIR (35% AMMONIUM SULPHATE, TRIS-HCL PH 7.2) VAPOUR DIFFUSSION HANGING DROP
|
Resolution 2.50 Å R-free 0.230 |
| 2XGC Crystal structure of a designed heterodimeric variant T-A(I)B of the tetracycline repressor Deposited 2010-06-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
51–208（158 aa）
Fragment：RESIDUES 1-50,51-208
|
Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.15 Å R-free 0.271 |
| 2XGC Crystal structure of a designed heterodimeric variant T-A(I)B of the tetracycline repressor Deposited 2010-06-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
51–208（158 aa）
Fragment：RESIDUES 1-50,51-208
|
Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.15 Å R-free 0.271 |
| 2XGD Crystal structure of a designed homodimeric variant T-A(L)A(L) of the tetracycline repressor Deposited 2010-06-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
51–208（158 aa）
Fragment：RESIDUES 1-50,51-208
|
Mutation：YES | CL CHLORIDE ION × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.25 Å R-free 0.259 |
| 2XGE Crystal structure of a designed heterodimeric variant T-A(A)B of the tetracycline repressor Deposited 2010-06-03 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
51–208（158 aa）
Fragment：RESIDUES 1-50,51-208
|
Not recorded | EDO 1,2-ETHANEDIOL × 2 NA SODIUM ION × 2 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.14 Å R-free 0.260 |
| 2XGE Crystal structure of a designed heterodimeric variant T-A(A)B of the tetracycline repressor Deposited 2010-06-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
51–208（158 aa）
Fragment：RESIDUES 1-50,51-208
|
Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.14 Å R-free 0.260 |
| 2XPU TetR(D) in complex with anhydrotetracycline. Deposited 2010-08-30 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–208（207 aa）
Fragment：RESIDUES 2-208
|
Not recorded | TDC 5A,6-ANHYDROTETRACYCLINE × 2 CL CHLORIDE ION × 4 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
50 MM MES PH 6.5, 1.1 M (NH4)2SO4, 100 MM NACL, 1 MM EDTA
|
Resolution 1.55 Å R-free 0.272 |
| 2XPU TetR(D) in complex with anhydrotetracycline. Deposited 2010-08-30 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–208（207 aa）
Fragment：RESIDUES 2-208
|
Not recorded | TDC 5A,6-ANHYDROTETRACYCLINE × 2 CL CHLORIDE ION × 4 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
50 MM MES PH 6.5, 1.1 M (NH4)2SO4, 100 MM NACL, 1 MM EDTA
|
Resolution 1.55 Å R-free 0.272 |
| 2XPV TetR(D) in complex with minocycline and magnesium. Deposited 2010-08-30 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–208（207 aa）
Fragment：RESIDUES 2-208
|
Not recorded | MIY (4S,4AS,5AR,12AS)-4,7-BIS(DIMETHYLAMINO)-3,10,12,12A-TETRAHYDROXY-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2- CARBOXAMIDE × 2 MG MAGNESIUM ION × 2 CL CHLORIDE ION × 16 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；12% PEG 6000, 2.0 M NACL, pH 8
|
Resolution 1.49 Å R-free 0.201 |
| 2XPW TetR(D) in complex with oxytetracycline and magnesium. Deposited 2010-08-30 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–208（207 aa）
Fragment：RESIDUES 2-208
|
Not recorded | OTC OXYTETRACYCLINE × 2 MG MAGNESIUM ION × 2 CL CHLORIDE ION × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
0.45 M NA-MALONATE, 0.45 M NA-SUCCINATE, 0.1 M NA-CACODYLATE PH 5.55
|
Resolution 1.44 Å R-free 0.205 |
| 2XRL Tet-repressor class D T103A with doxycycline Deposited 2010-09-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–208（206 aa）
Fragment：RESIDUES 2-208
|
Mutation：YES | CL CHLORIDE ION × 4 DXT (4S,4AR,5S,5AR,6R,12AS)-4-(DIMETHYLAMINO)-3,5,10,12,12A-PENTAHYDROXY-6-METHYL-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2-CARBOXAMIDE × 2 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；HANGING DROP VAPOR DIFFUSION WITH 2 UL (14 MG/ML PROTEIN) AND 2 UL (0.6 M NACL, 15 % PEG 4000, 0.1 M MES PH 6.5). CRYSTAL WAS CRYOCOOLED WITH PARAFFIN OIL AS CRYOPROTECTANT.
|
Resolution 1.85 Å R-free 0.279 |
| 3FK6 Crystal structure of TetR triple mutant (H64K, S135L, S138I) Deposited 2008-12-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
51–208（158 aa）
Fragment：DNA-binding domain (residues 1-50) and the effector-binding domain (residues 51-208)
Chain B
51–208（158 aa）
Fragment：DNA-binding domain (residues 1-50) and the effector-binding domain (residues 51-208)
|
Mutation：H64K, S135L, S138I Mutation：H64K, S135L, S138I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；292 K；1M dipotassium hydrogen phosphate, 200mM sodium chloride, 50mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 2.10 Å R-free 0.262 |
| 3FK7 Crystal structure of TetR triple mutant (H64K, S135L, S138I) in complex with 4-ddma-atc Deposited 2008-12-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
51–208（158 aa）
Fragment：DNA-binding domain (residues 1-50) and the effector-binding domain (residues 51-208)
Chain B
51–208（158 aa）
Fragment：DNA-binding domain (residues 1-50) and the effector-binding domain (residues 51-208)
|
Mutation：H64K, S135L, S138I Mutation：H64K, S135L, S138I | MG MAGNESIUM ION × 2 4DM (4aS,12aS)-3,10,11,12a-tetrahydroxy-6-methyl-1,12-dioxo-1,4,4a,5,12,12a-hexahydrotetracene-2-carboxamide × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；292 K；1M dipotassium hydrogen phosphate, 200mM sodium chloride, 50mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 2.06 Å R-free 0.261 |
| 3ZQF Structure of Tetracycline repressor in complex with antiinducer peptide-TAP1 Deposited 2011-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
188–208（21 aa）
Fragment：RESIDUES 1-187,188-208
|
Mutation：YES | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6；RESERVOIR: 3 M NACL, 0.1 M BIS-TRIS, PH 6.
|
Resolution 2.56 Å R-free 0.247 |
| 3ZQG Structure of Tetracycline repressor in complex with antiinducer peptide-TAP2 Deposited 2011-06-09 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
188–208（21 aa）
Fragment：RESIDUES 1-187,188-208
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；RESERVOIR: 3 M NACL, 0.1 M BIS-TRIS, PH 6.5
|
Resolution 2.45 Å R-free 0.234 |
| 3ZQH Structure of Tetracycline repressor in complex with inducer peptide- TIP3 Deposited 2011-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
188–208（21 aa）
Fragment：RESIDUES 1-187,188-208
|
Mutation：YES | EDO 1,2-ETHANEDIOL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 9；0.2 M NACL, 0.1 M TRIS PH 9.0, 25% PEG 3350.
|
Resolution 1.60 Å R-free 0.224 |
| 3ZQI Structure of Tetracycline repressor in complex with inducer peptide- TIP2 Deposited 2011-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
188–208（21 aa）
Fragment：RESIDUES 1-187,188-208
Chain B
188–208（21 aa）
Fragment：RESIDUES 1-187,188-208
|
Mutation：YES Mutation：YES | EDO 1,2-ETHANEDIOL × 5 MG MAGNESIUM ION × 1 PG4 TETRAETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；0.2 M MAGNESIUM ACETATE, 0.1 M SODIUM CACODYLATE, PH 7.0, 20% PEG 8000.
|
Resolution 1.50 Å R-free 0.195 |
| 4ABZ TetR(D) in Complex with Tigecycline and Magnesium, co-crystallized Deposited 2011-12-12 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain AAA
2–208（207 aa）
|
Mutation：A2S | T1C TIGECYCLINE × 2 MG MAGNESIUM ION × 2 CL CHLORIDE ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；291 K；Protein: 10.0 mg/mL TetR(D), 0.2M NaCl, 50 mM Tris/HCl, pH 8.0, 2mM [TigeTC], 2mM MgCl2.
Precipitant: 1M (NH4)2SO4, 0.2M NaCl, 0.05M Tris/HCl, pH 8.0, 10mM MgCl2.
Protein/precipitant 2+2 microL, against 0.5mL Precipitant.
|
Resolution 1.89 Å R-free 0.258 |
| 4AUX Tet repressor class D in complex with 9-nitrotetracycline Deposited 2012-05-22 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–208（207 aa）
Fragment：RESIDUES 2-208
|
Not recorded | XTC (4S,4aS,5aR,12aS)-4-(dimethylamino)-3,10,12,12a-tetrahydroxy-9-nitro-1,11-dioxo-1,4,4a,5,5a,6,11,12a-octahydrotetracene-2-carboxamide × 2 MG MAGNESIUM ION × 2 CL CHLORIDE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；291 K；1 M (NH4)2S04, 200 MM NACL, 5 MM MGCL2 AND 20 MM TRIS-HCL, PH 8.0 WITH 10 MG/ML TETR, 2 MM 9-NITROTC AND 5 MM MGCL2, HANGING DROP, TEMPERATURE 291K
|
Resolution 2.25 Å R-free 0.258 |
| 4B1R Tetracycline repressor class D mutant H100A in complex with iso-7- Chlortetracycline Deposited 2012-07-12 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–208（206 aa）
Fragment：RESIDUES 3-208
|
Mutation：YES | ITC ISO-7-CHLORTETRACYCLINE × 2 CL CHLORIDE ION × 10 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；0.1 M MES, PH 6.5, 0.6 M LI2SO4, 16.7 % PEG 4000
|
Resolution 1.95 Å R-free 0.260 |
| 4B3A Tetracycline repressor class D mutant H100A in complex with tetracycline Deposited 2012-07-23 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–208（207 aa）
Fragment：RESIDUES 2-208
|
Mutation：YES | TAC TETRACYCLINE × 2 CL CHLORIDE ION × 6 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；0.5 M AMMONIUM SULPHATE, 1 M LITHIUM SULPHATE, 0.1 M MES PH 6.5
|
Resolution 1.70 Å R-free 0.250 |
| 4D7M TetR(D) in complex with anhydrotetracycline and magnesium Deposited 2014-11-25 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–208（207 aa）
|
Not recorded | TDC 5A,6-ANHYDROTETRACYCLINE × 2 MG MAGNESIUM ION × 2 SO4 SULFATE ION × 2 CL CHLORIDE ION × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 9；50 MM TRIS-HCL PH 9.0, 1 M (NH4)2SO4, 70 MM NACL, 10 MM MGCL2
|
Resolution 1.55 Å R-free 0.217 |
| 4V2F Tetracycline repressor TetR(D), unliganded Deposited 2014-10-09 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–208（206 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；PH 8.0
|
Resolution 2.40 Å R-free 0.278 |
| 4V2G Tetracycline repressor TetR(D) bound to chlortetracycline and iso- chlortetracycline Deposited 2014-10-09 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–208（206 aa）
Chain B
3–208（206 aa）
|
Not recorded | ITC ISO-7-CHLORTETRACYCLINE × 1 CTC 7-CHLOROTETRACYCLINE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；PH 8.0
|
Resolution 2.71 Å R-free 0.252 |
| 6FPL TETR(D) E147A MUTANT IN COMPLEX WITH TETRACYCLINE AND MAGNESIUM Deposited 2018-02-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–208（207 aa）
|
Mutation：A2S, E147A | TAC TETRACYCLINE × 2 MG MAGNESIUM ION × 2 CL CHLORIDE ION × 12 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；Precipitant 0.1M Na-citrat, 0.8M Li2SO4, 0.5M (NH4)2SO4,
0.1mL Protein (10.19mg/ml, 0.1M MES pH 6.5) + 0.1mL 2mM Tc + 0.001mL 3M MgCl2
1:4 Precipitant/Protein
|
Resolution 1.60 Å R-free 0.221 |
| 6FPM TetR(D) T103A mutant in complex with tetracycline and magnesium Deposited 2018-02-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–208（207 aa）
|
Mutation：A2S, T103A | TAC TETRACYCLINE × 2 MG MAGNESIUM ION × 2 CL CHLORIDE ION × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；0.1M MES pH 6.5, 24% PEG 400, 70mM MgCl2.
2mM TC, 14.55mg/ml protein
|
Resolution 1.85 Å R-free 0.267 |
| 6FTS TETR(D) N82A MUTANT IN COMPLEX WITH PEG4 Deposited 2018-02-23 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–208（206 aa）
|
Not recorded | PG4 TETRAETHYLENE GLYCOL × 2 CL CHLORIDE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；Precipitant: 2M (NH4)2SO4, 6% PEG400, 0.1M Tris pH8.5.
19.65mg/ml Protein, 2mM Tetracycline, 3M MgCl2.
|
Resolution 2.00 Å R-free 0.272 |
| 6QJW TetR(D) T103A mutant in complex with 7-chlortetracycline and magnesium Deposited 2019-01-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–208（207 aa）
|
Mutation：A2S, T103A | CTC 7-CHLOROTETRACYCLINE × 2 MG MAGNESIUM ION × 2 CL CHLORIDE ION × 10 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；295 K；Precipitant: 30%PEG 400, 0.1M MES pH 6.5, 60 mM MgCl2. Protein: 12.15 mg/mL protein in 100mM NaCl, 50 mM Tris buffer, pH 8.0, 4mM 7ClTc, 3M MgCl2. Protein/precipitant 1/1
|
Resolution 2.10 Å R-free 0.290 |
| 6QJX TetR(D) T103A mutant in complex with minocycline and magnesium Deposited 2019-01-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–208（207 aa）
|
Mutation：A2S, T103A | MIY (4S,4AS,5AR,12AS)-4,7-BIS(DIMETHYLAMINO)-3,10,12,12A-TETRAHYDROXY-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2- CARBOXAMIDE × 2 MG MAGNESIUM ION × 2 CL CHLORIDE ION × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；Precipitant: 0.1 M MES pH 6.5, 24% PEG 400, 70 mM MgCl2. Protein: 14.55 mg/mL protein in 100mM NaCl, 50 mM Tris buffer, pH 8.0, with 2mM MinoTc, 0.38 uL 3M MgCl2. Precipitant/protein 1/4.
|
Resolution 2.10 Å R-free 0.280 |
| 6RBL TETR(D) E147A MUTANT IN COMPLEX WITH DOXYCYCLINE AND MAGNESIUM Deposited 2019-04-10 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–208（207 aa）
|
Mutation：A2S, E147A | DXT (4S,4AR,5S,5AR,6R,12AS)-4-(DIMETHYLAMINO)-3,5,10,12,12A-PENTAHYDROXY-6-METHYL-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2-CARBOXAMIDE × 2 MG MAGNESIUM ION × 2 CL CHLORIDE ION × 12 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；295 K；Precipitant: 2.0M (NH4)2SO4, 3% PEG 400, 0.1M MES pH 6.5,
Protein: 0.1mL protein, 0.1,L 2mM doxyTc, 0.001mL 3M MgCl2, 100mM NaCl, 50mM Tris pH8.0,
1/3 protein/precipitant
|
Resolution 1.90 Å R-free 0.246 |
| 6RBM TETR(D) E147A MUTANT IN COMPLEX WITH MINOCYCLINE AND MAGNESIUM Deposited 2019-04-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–208（207 aa）
|
Mutation：A2S, E147A | MIY (4S,4AS,5AR,12AS)-4,7-BIS(DIMETHYLAMINO)-3,10,12,12A-TETRAHYDROXY-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2- CARBOXAMIDE × 2 MG MAGNESIUM ION × 2 CL CHLORIDE ION × 12 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；295 K；precipitant: 0.5M (NH4)2SO4, 0.68M Li2SO4, 0.1M Na-citrate,
protein: 0.1mL protein, 0.1mL 2mM minoTc, 0.0005mL 3M MgCl2, 100 mM NaCl, 50 mM Tris pH 8.0,
ratio 2/2
|
Resolution 2.05 Å R-free 0.263 |
| 6RCR TETR(D) H100A MUTANT IN COMPLEX WITH DOXYCYCLINE AND MAGNESIUM Deposited 2019-04-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–208（207 aa）
|
Mutation：A2S, H100A | DXT (4S,4AR,5S,5AR,6R,12AS)-4-(DIMETHYLAMINO)-3,5,10,12,12A-PENTAHYDROXY-6-METHYL-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2-CARBOXAMIDE × 2 MG MAGNESIUM ION × 2 CL CHLORIDE ION × 10 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；295 K；precipitant: 0.5M (NH4)2SO4, 1.0M Li2SO4, 0.1 M MES pH 6.5,
protein: 0.050mL protein(10mg/mL), 0.050mL 2mM doxyTc, 0.0005mL 3M MgCl2, 100mM NaCl, 50mM Tris pH 8.0,
371 protein/precipitant
|
Resolution 2.05 Å R-free 0.257 |
| 6RGX TETR(D) N82A MUTANT IN COMPLEX WITH DOXYCYCLINE AND MAGNESIUM Deposited 2019-04-17 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–208（206 aa）
Chain B
3–208（206 aa）
|
Mutation：A2S, N82A Mutation：A2S, N82A | CL CHLORIDE ION × 10 PG4 TETRAETHYLENE GLYCOL × 1 PGE TRIETHYLENE GLYCOL × 1 DXT (4S,4AR,5S,5AR,6R,12AS)-4-(DIMETHYLAMINO)-3,5,10,12,12A-PENTAHYDROXY-6-METHYL-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2-CARBOXAMIDE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；295 K；precipitant: 0.1 M HEPES pH 7.5, 1.75M (NH4)2SO4, 5% PEG 400, 0.2M NaCl,
protein: 0.1mL protein, 0.3mL 2mM doxyTc, 0.001mL 3M MgCl2, 100mM NaCl, 50mM Tris, pH 8.0,
Protein/precipitant 2/2
|
Resolution 1.80 Å R-free 0.243 |
| 6YR1 TetR(D) soaked with Tigecycline I4(1)22 Deposited 2020-04-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain AAA
2–208（207 aa）
|
Mutation：A2S | T1C TIGECYCLINE × 2 MG MAGNESIUM ION × 2 CL CHLORIDE ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；Native crystal soaked in 1M (NH4)2SO4, 0.2M NaCl, 0.05M Tris/HCl, pH 7.0, 0.0142 mM Tigecycline, 2mM MgCl2, 22days
|
Resolution 2.30 Å R-free 0.279 |
| 6YR2 TetR(D) soaked with Tigecycline P4(1)2(1)2 Deposited 2020-04-19 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain AAA
2–208（207 aa）
Chain BBB
2–208（207 aa）
|
Mutation：A2S Mutation：A2S | SO4 SULFATE ION × 2 CL CHLORIDE ION × 8 T1C TIGECYCLINE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；Native crystal soaked in 1M (NH4)2SO4, 0.2M NaCl, 0.05M Tris/HCl, pH 7.0, 0.142 mM TigeTC, 2mM MgCl2, 14hours.
|
Resolution 1.95 Å R-free 0.237 |
45 other PDB entries and 51 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | TETR4_ECOLI |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–207； UniProt 1–207 |
