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2xnf

The Mediator Med25 activator interaction domain: Structure and cooperative binding of VP16 subdomains

Method: SOLUTION NMR
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1. Protein Identity and Related Structures Protein Identity & Related Structures

MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 25

HOMO SAPIENS

UniProt Q71SY5

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein monomer Monomer Protein 1 No other associated polymer Consistent with protein count

Other States of the Same Protein in the Database

Monomer
2ky6 2l23 2l6u 2xnf

4 assemblies

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
7emf 7lbm 8gxq 8gxs 8t9d 9ccv

6 assemblies

View Construct and Data Evidence
UniProt name MED25_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 2–151； UniProt 394–543

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id2xnf
Deposition date deposition_date2010-08-02
Structure title titleThe Mediator Med25 activator interaction domain: Structure and cooperative binding of VP16 subdomains
Keywords keywordsTRANSCRIPTION, ACTIVATED TRANSCRIPTION, MEDIATOR; TRANSCRIPTION
Experimental Method methodSOLUTION NMR
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2xnf__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2xnf__assembly_1__model_1 | I(q)

10-2 10-1 105 106 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

2xnf__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）17.11 Å
Rg (electron density)15.55 Å
Total Rg16.79 Å
Atom count2583
Residues159
Excluded volume23022 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 2xnf__assembly_1__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 2 2xnf__assembly_1__model_2 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 3 2xnf__assembly_1__model_3 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 4 2xnf__assembly_1__model_4 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 5 2xnf__assembly_1__model_5 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 6 2xnf__assembly_1__model_6 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 7 2xnf__assembly_1__model_7 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 8 2xnf__assembly_1__model_8 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 9 2xnf__assembly_1__model_9 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 10 2xnf__assembly_1__model_10 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (1)

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6. Fold Classification (SCOP + CATH) 1 domains

CATH v4.4 (1 domains)

Domain ID domain_id2xnfA00
Class class2 — Mainly Beta
Architecture architecture40 — Beta Barrel
Topology topology290 — Ku70; Chain: A; Domain 2
Homologous superfamily homologous superfamily30 — Mediator complex subunit 25, ACID domain
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7. Citations (1)