3b78

Structure of the eEF2-ExoA(R551H)-NAD+ complex

Method: X-RAY DIFFRACTION Dmax: 197.0 Å Quality: REASONABLE

1. 蛋白身份与相关结构 Protein Identity & Related Structures

Elongation factor 2

物种未注明

UniProt P32324

当前结构中的状态

Assembly 聚集状态 构建体 突变与修饰 配体、离子与共同组分 实验方法与环境 结构质量
1 蛋白异源复合物 异源复合物 蛋白 × 2 PDB 声明:dimeric(2) 与蛋白拷贝数一致 链 A; UniProt 1–842 非标准单体:是(mmCIF未提供具体位点) Exotoxin A × 1 (P11439) NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray结晶条件:VAPOR DIFFUSION, SITTING DROP;pH 7.2;293 K;7% PEG-10000, 3.5mM MPD, 100 mM HEPES, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K 分辨率 2.50 Å R-free 0.242
2 蛋白异源复合物 异源复合物 蛋白 × 2 PDB 声明:dimeric(2) 与蛋白拷贝数一致 链 C; UniProt 1–842 非标准单体:是(mmCIF未提供具体位点) Exotoxin A × 1 (P11439) NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray结晶条件:VAPOR DIFFUSION, SITTING DROP;pH 7.2;293 K;7% PEG-10000, 3.5mM MPD, 100 mM HEPES, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K 分辨率 2.50 Å R-free 0.242
3 蛋白异源复合物 异源复合物 蛋白 × 2 PDB 声明:dimeric(2) 与蛋白拷贝数一致 链 E; UniProt 1–842 非标准单体:是(mmCIF未提供具体位点) Exotoxin A × 1 (P11439) NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray结晶条件:VAPOR DIFFUSION, SITTING DROP;pH 7.2;293 K;7% PEG-10000, 3.5mM MPD, 100 mM HEPES, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K 分辨率 2.50 Å R-free 0.242

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly, “相对当前条目”直接指出证据层面的不同;没有差异标签表示当前已读取字段一致。

共 41 个其他 PDB 条目、57 个 assembly。 打开独立比较页并筛选聚集状态

查看构建体与数据证据
UniProt名称 EF2_YEAST
Isoform
PDB实体 1
链与序列区间 作者链 A; PDB构建体 1–842; UniProt 1–842 作者链 C; PDB构建体 1–842; UniProt 1–842 作者链 E; PDB构建体 1–842; UniProt 1–842

Exotoxin A

Pseudomonas aeruginosa

UniProt P11439

当前结构中的状态

Assembly 聚集状态 构建体 突变与修饰 配体、离子与共同组分 实验方法与环境 结构质量
1 蛋白异源复合物 异源复合物 蛋白 × 2 PDB 声明:dimeric(2) 与蛋白拷贝数一致 链 B; UniProt 425–630 片段:catalytic domain 突变:R551H Elongation factor 2 × 1 (P32324) NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray结晶条件:VAPOR DIFFUSION, SITTING DROP;pH 7.2;293 K;7% PEG-10000, 3.5mM MPD, 100 mM HEPES, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K 分辨率 2.50 Å R-free 0.242
2 蛋白异源复合物 异源复合物 蛋白 × 2 PDB 声明:dimeric(2) 与蛋白拷贝数一致 链 D; UniProt 425–630 片段:catalytic domain 突变:R551H Elongation factor 2 × 1 (P32324) NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray结晶条件:VAPOR DIFFUSION, SITTING DROP;pH 7.2;293 K;7% PEG-10000, 3.5mM MPD, 100 mM HEPES, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K 分辨率 2.50 Å R-free 0.242
3 蛋白异源复合物 异源复合物 蛋白 × 2 PDB 声明:dimeric(2) 与蛋白拷贝数一致 链 F; UniProt 425–630 片段:catalytic domain 突变:R551H Elongation factor 2 × 1 (P32324) NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray结晶条件:VAPOR DIFFUSION, SITTING DROP;pH 7.2;293 K;7% PEG-10000, 3.5mM MPD, 100 mM HEPES, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K 分辨率 2.50 Å R-free 0.242

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly, “相对当前条目”直接指出证据层面的不同;没有差异标签表示当前已读取字段一致。

共 8 个其他 PDB 条目、15 个 assembly。 打开独立比较页并筛选聚集状态

查看构建体与数据证据
UniProt名称 TOXA_PSEAE
Isoform
PDB实体 2
链与序列区间 作者链 B; PDB构建体 2–207; UniProt 425–630 作者链 D; PDB构建体 2–207; UniProt 425–630 作者链 F; PDB构建体 2–207; UniProt 425–630

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。 链映射与序列区间收在“数据证据”中;数据库内部编号、导入时间和 assembly 操作表达式仅用于维护,因此不在读者页面展示。

SAXS 散射曲线 SAXS Profile

SAXS profile for 3b78

P(r) 距离分布 P(r) Distribution

P(r) distribution for 3b78
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2. 结构基本信息 2. Structure Basics

条目编号 entry_id3b78
沉积日期 deposition_date2007-10-30
结构标题 titleStructure of the eEF2-ExoA(R551H)-NAD+ complex
关键词 keywords;elongation factor, toxin, ADP-ribosylation, toxin-substrate complex, Cytoplasm, GTP-binding, Nucleotide-binding, Phosphoprotein, Protein biosynthesis, RNA-binding, rRNA-binding, Glycosyltransferase, NAD, Transferase, BIOSYNTHETIC PROTEIN-TRANSFERASE COMPLEX ;; BIOSYNTHETIC PROTEIN/TRANSFERASE
实验方法 methodX-RAY DIFFRACTION

3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier64.13
回转半径 Rg (电子) rg_electron64.69
零角强度 I(0) i01634730000.00
分子量 molecular_weight342470.0 kDa
排除体积 excluded_volume430130 ų
包络体积 envelope_volume668800 ų
水化壳体积 shell_volume91695 ų
包络直径 envelope_diameter212.2
壳层 Rg shell_rg58.30
包络 Rg envelope_rg62.61
形状 Rg shape_rg64.71
总 Rg total_rg64.48
总原子数 total_atoms24118
残基数 n_residues3087
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax197.0
Rg (实空间) rg_real64.49
Rg 误差 (实空间) rg_real_error1.96
I(0) (实空间) i0_real1.6350e+09
I(0) 误差 (实空间) i0_real_error3.5370e+07
Rg (倒空间) rg_reciprocal63.76
I(0) (倒空间) i0_reciprocal1633000000.0000
解质量估计 total_estimate0.6057
解质量评级 solution_quality REASONABLE a REASONABLE solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary73.9
偏度 Skewness skewness0.366
峰度 Kurtosis kurtosis-0.528
角度范围 angular_range— – 0.1200 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha40390000.0000
实空间数据点数 n_real_points25
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.942; Stabil: 0.999; Sysdev: 0.013; Positv: 1.000; Valcen: 0.992; Smooth: 0.016

5. 晶体学与实验 5. Crystallography & Experiment

6. 实体与聚合物信息 Entities & Polymers (4)

7. 折叠分类 (SCOP + CATH) 42 domains

SCOP 2.08 (21 domains)

结构域编号 domain_idd3b78a1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
超家族 Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
家族 Family familyc.37.1.8 — G proteins
结构域编号 domain_idd3b78a2
类 Class classb — All beta proteins
折叠类型 Fold foldb.43 — Reductase/isomerase/elongation factor common domain
超家族 Superfamily superfamilyb.43.3 — Translation proteins
家族 Family familyb.43.3.1 — Elongation factors
结构域编号 domain_idd3b78a3
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.11 — EF-G C-terminal domain-like
家族 Family familyd.58.11.1 — EF-G/eEF-2 domains III and V
结构域编号 domain_idd3b78a4
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.14 — Ribosomal protein S5 domain 2-like
超家族 Superfamily superfamilyd.14.1 — Ribosomal protein S5 domain 2-like
家族 Family familyd.14.1.1 — Translational machinery components
结构域编号 domain_idd3b78a5
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.11 — EF-G C-terminal domain-like
家族 Family familyd.58.11.1 — EF-G/eEF-2 domains III and V
结构域编号 domain_idd3b78b1
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.166 — ADP-ribosylation
超家族 Superfamily superfamilyd.166.1 — ADP-ribosylation
家族 Family familyd.166.1.1 — ADP-ribosylating toxins
结构域编号 domain_idd3b78b2
类 Class classl — Artifacts
折叠类型 Fold foldl.1 — Tags
超家族 Superfamily superfamilyl.1.1 — Tags
家族 Family familyl.1.1.1 — Tags
结构域编号 domain_idd3b78c1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
超家族 Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
家族 Family familyc.37.1.8 — G proteins
结构域编号 domain_idd3b78c2
类 Class classb — All beta proteins
折叠类型 Fold foldb.43 — Reductase/isomerase/elongation factor common domain
超家族 Superfamily superfamilyb.43.3 — Translation proteins
家族 Family familyb.43.3.1 — Elongation factors
结构域编号 domain_idd3b78c3
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.11 — EF-G C-terminal domain-like
家族 Family familyd.58.11.1 — EF-G/eEF-2 domains III and V
结构域编号 domain_idd3b78c4
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.14 — Ribosomal protein S5 domain 2-like
超家族 Superfamily superfamilyd.14.1 — Ribosomal protein S5 domain 2-like
家族 Family familyd.14.1.1 — Translational machinery components
结构域编号 domain_idd3b78c5
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.11 — EF-G C-terminal domain-like
家族 Family familyd.58.11.1 — EF-G/eEF-2 domains III and V
结构域编号 domain_idd3b78d1
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.166 — ADP-ribosylation
超家族 Superfamily superfamilyd.166.1 — ADP-ribosylation
家族 Family familyd.166.1.1 — ADP-ribosylating toxins
结构域编号 domain_idd3b78d2
类 Class classl — Artifacts
折叠类型 Fold foldl.1 — Tags
超家族 Superfamily superfamilyl.1.1 — Tags
家族 Family familyl.1.1.1 — Tags
结构域编号 domain_idd3b78e1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
超家族 Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
家族 Family familyc.37.1.8 — G proteins
结构域编号 domain_idd3b78e2
类 Class classb — All beta proteins
折叠类型 Fold foldb.43 — Reductase/isomerase/elongation factor common domain
超家族 Superfamily superfamilyb.43.3 — Translation proteins
家族 Family familyb.43.3.1 — Elongation factors
结构域编号 domain_idd3b78e3
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.11 — EF-G C-terminal domain-like
家族 Family familyd.58.11.1 — EF-G/eEF-2 domains III and V
结构域编号 domain_idd3b78e4
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.14 — Ribosomal protein S5 domain 2-like
超家族 Superfamily superfamilyd.14.1 — Ribosomal protein S5 domain 2-like
家族 Family familyd.14.1.1 — Translational machinery components
结构域编号 domain_idd3b78e5
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.11 — EF-G C-terminal domain-like
家族 Family familyd.58.11.1 — EF-G/eEF-2 domains III and V
结构域编号 domain_idd3b78f1
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.166 — ADP-ribosylation
超家族 Superfamily superfamilyd.166.1 — ADP-ribosylation
家族 Family familyd.166.1.1 — ADP-ribosylating toxins
结构域编号 domain_idd3b78f2
类 Class classl — Artifacts
折叠类型 Fold foldl.1 — Tags
超家族 Superfamily superfamilyl.1.1 — Tags
家族 Family familyl.1.1.1 — Tags

CATH v4.4 (21 domains)

结构域编号 domain_id3b78A01
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
结构域编号 domain_id3b78A02
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology1430 — Yeast translation eEF2 (G' domain)
同源超家族 H-superfamily homologous superfamily10 — Yeast translation eEF2 (G' domain)
结构域编号 domain_id3b78A03
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology30 — Elongation Factor Tu (Ef-tu); domain 3
同源超家族 H-superfamily homologous superfamily10 — Translation factors
结构域编号 domain_id3b78A04
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily870 — Elongation Factor G (Translational Gtpase), domain 3
结构域编号 domain_id3b78A05
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology230 — Ribosomal Protein S5; domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id3b78A06
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily240
结构域编号 domain_id3b78B00
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology175 — Diphtheria Toxin; domain 1
同源超家族 H-superfamily homologous superfamily10 — Diphtheria Toxin, domain 1
结构域编号 domain_id3b78C01
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
结构域编号 domain_id3b78C02
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology1430 — Yeast translation eEF2 (G' domain)
同源超家族 H-superfamily homologous superfamily10 — Yeast translation eEF2 (G' domain)
结构域编号 domain_id3b78C03
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology30 — Elongation Factor Tu (Ef-tu); domain 3
同源超家族 H-superfamily homologous superfamily10 — Translation factors
结构域编号 domain_id3b78C04
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily870 — Elongation Factor G (Translational Gtpase), domain 3
结构域编号 domain_id3b78C05
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology230 — Ribosomal Protein S5; domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id3b78C06
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily240
结构域编号 domain_id3b78D00
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology175 — Diphtheria Toxin; domain 1
同源超家族 H-superfamily homologous superfamily10 — Diphtheria Toxin, domain 1
结构域编号 domain_id3b78E01
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
结构域编号 domain_id3b78E02
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology1430 — Yeast translation eEF2 (G' domain)
同源超家族 H-superfamily homologous superfamily10 — Yeast translation eEF2 (G' domain)
结构域编号 domain_id3b78E03
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology30 — Elongation Factor Tu (Ef-tu); domain 3
同源超家族 H-superfamily homologous superfamily10 — Translation factors
结构域编号 domain_id3b78E04
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily870 — Elongation Factor G (Translational Gtpase), domain 3
结构域编号 domain_id3b78E05
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology230 — Ribosomal Protein S5; domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id3b78E06
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily240
结构域编号 domain_id3b78F00
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology175 — Diphtheria Toxin; domain 1
同源超家族 H-superfamily homologous superfamily10 — Diphtheria Toxin, domain 1

8. 引用文献 (1)

9. 文件与曲线 (10)