Chymotrypsin-like elastase family member 1
OrganismNot specified
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain A； UniProt 27–266 | Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；291 K；The initial concentration of the protein was 20 mg/ml in 10% glycerol solution. The reservoir contained a 250 mM Na2SO4. For cryo-protection, it was supplemented with 25% glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K | Resolution 1.40 Å R-free 0.148 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 3MU5 | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1B0E CRYSTAL STRUCTURE OF PORCINE PANCREATIC ELASTASE WITH MDL 101,146 Deposited 1998-11-09 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SEI 1-{3-METHYL-2-[4-(MORPHOLINE-4-CARBONYL)-BENZOYLAMINO]-BUTYRYL}-PYRROLIDINE-2-CARBOXYLIC ACID (3,3,4,4,4-PENTAFLUORO-1-ISOPROPYL-2-OXO-BUTYL)-AMIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；SITTING DROP PROCEDURE. PROTEIN DROP: 13 MG/ML PPE (SERVA) 9.3 MM SODIUM ACETATE (PH 5.0), 22.4 MM SODIUM SULFATE, 2.2 MM MDL 103,139 IN DMF RESERVOIR: 10 MM SODIUM ACETATE (PH 5.0), 20 MM SODIUM SULFATE., vapor diffusion - sitting drop
|
Resolution 1.80 Å |
| 1BMA BENZYL METHYL AMINIMIDE INHIBITOR COMPLEXED TO PORCINE PANCREATIC ELASTASE Deposited 1995-05-01 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 0QH (1R)-1-benzyl-1-methyl-1-(2-{[4-(1-methylethyl)phenyl]amino}-2-oxoethyl)-2-{(2S)-4-methyl-2-[(trifluoroacetyl)amino]pentanoyl}diazanium × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
THE INHIBITOR WAS SOAKED INTO THE GROWN CRYSTALS USING A
4 MM STOCK SOLUTION OF THE INHIBITOR IN 10% ACETONITRILE.
|
Resolution 1.80 Å |
| 1BTU PORCINE PANCREATIC ELASTASE COMPLEXED WITH (3S, 4R)-1-TOLUENESULPHONYL-3-ETHYL-AZETIDIN-2-ONE-4-CARBOXYLIC ACID Deposited 1998-09-01 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 2BL (3R)-3-ethyl-N-[(4-methylphenyl)sulfonyl]-L-aspartic acid × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5；pH 5.0
|
Resolution 1.60 Å R-free 0.220 |
| 1C1M PORCINE ELASTASE UNDER XENON PRESSURE (8 BAR) Deposited 1999-07-22 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 XE XENON × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.5；1.4 MG ENZYME IN 0.1 ML ACETATE BUFFER (0.05M) PLUS 0.0001 ML PRECIPITATING AGENT (SODIUM SULPHATE 1M), pH 5.50
|
Resolution 2.20 Å R-free 0.201 |
| 1E34 PORCINE PANCREATIC ELASTASE COMPLEXED WITH (3S, 4S)N-PARA- TOLUENESULPHONYL-3-ETHYL-4-(CARBOXYLIC ACID) PYRROLIDIN-2-ONE SOAKED IN PH 9 BUFFER FOR ONE MINUTE Deposited 2000-06-06 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 TPX (2S,3S)-3-FORMYL-2-({[(4-METHYLPHENYL)SULFONYL]AMINO}METHYL)PENTANOIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 9；25MM SODIUM SULPHATE, 25MM SODIUM ACETATE, PH 5.0; THEN SOAKED IN 25 MM SODIUM SULPHATE, 50MM SODIUM ACETATE, 20 MG/ML GAMMA-LACTAM, 10% (V/V) DMSO, PH 5.0 FOR 24 HOURS
|
Resolution 1.80 Å R-free 0.218 |
| 1E35 PORCINE PANCREATIC ELASTASE COMPLEXED WITH (3S, 4S)N-PARA-TOLUENESULPHONYL -3-ETHYL-4-(CARBOXYLIC ACID)PYRROLIDIN-2-ONE SOAKED IN PH 9 BUFFER FOR TWO MINUTES Deposited 2000-06-06 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 TPX (2S,3S)-3-FORMYL-2-({[(4-METHYLPHENYL)SULFONYL]AMINO}METHYL)PENTANOIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 9；25MM SODIUM SULPHATE, 25MM SODIUM ACETATE, PH 5.0; THEN SOAKED IN 25 MM SODIUM SULPHATE, 50MM SODIUM ACETATE, 20 MG/ML GAMMA-LACTAM, 10% (V/V) DMSO, PH 5.0 FOR 24 HOURS
|
Resolution 1.90 Å R-free 0.225 |
| 1E36 PORCINE PANCREATIC ELASTASE COMPLEXED WITH (3S, 4S)N-PARA-NITROBENZENESULPHONYL -3-ETHYL-4-(CARBOXYLIC ACID)PYRROLIDIN-2-ONE Deposited 2000-06-06 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 TPY (2S,3S)-3-FORMYL-2-({[(4-NITROPHENYL)SULFONYL]AMINO}METHYL)PENTANOIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5；25MM SODIUM SULPHATE, 25MM SODIUM ACETATE, PH 5.0; THEN SOAKED IN 25 MM SODIUM SULPHATE, 50MM SODIUM ACETATE, 20 MG/ML GAMMA-LACTAM, 10% (V/V) DMSO, PH 5.0 FOR 24 HOURS
|
Resolution 1.70 Å R-free 0.220 |
| 1E37 PORCINE PANCREATIC ELASTASE COMPLEXED WITH (3S, 4S)N-PARA-NITROBENZENESULPHONYL -3-ETHYL-4-(CARBOXYLIC ACID)PYRROLIDIN-2-ONE SOAKED IN PH 9 BUFFER FOR 1 MINUTE Deposited 2000-06-06 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 TPY (2S,3S)-3-FORMYL-2-({[(4-NITROPHENYL)SULFONYL]AMINO}METHYL)PENTANOIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 9；25MM SODIUM SULPHATE, 25MM SODIUM ACETATE, PH 5.0; THEN SOAKED IN 25 MM SODIUM SULPHATE, 50MM SODIUM ACETATE, 20 MG/ML GAMMA-LACTAM, 10% (V/V) DMSO, PH 5.0 FOR 24 HOURS
|
Resolution 1.75 Å R-free 0.230 |
| 1E38 PORCINE PANCREATIC ELASTASE COMPLEXED WITH (3S, 4S)N-PARA-NITROBENZENESULPHONYL -3-ETHYL-4-(CARBOXYLIC ACID)PYRROLIDIN-2-ONE SOAKED IN PH 9 BUFFER FOR 2 MINUTES Deposited 2000-06-06 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 TPY (2S,3S)-3-FORMYL-2-({[(4-NITROPHENYL)SULFONYL]AMINO}METHYL)PENTANOIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 9；25MM SODIUM SULPHATE, 25MM SODIUM ACETATE, PH 5.0; THEN SOAKED IN 25 MM SODIUM SULPHATE, 50MM SODIUM ACETATE, 20 MG/ML GAMMA-LACTAM, 10% (V/V) DMSO, PH 5.0 FOR 24 HOURS
|
Resolution 1.70 Å R-free 0.220 |
| 1EAI COMPLEX OF ASCARIS CHYMOTRPSIN/ELASTASE INHIBITOR WITH PORCINE ELASTASE Deposited 1999-03-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
27–266（240 aa）
Chain B
27–266（240 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 2.40 Å |
| 1EAI COMPLEX OF ASCARIS CHYMOTRPSIN/ELASTASE INHIBITOR WITH PORCINE ELASTASE Deposited 1999-03-25 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
27–266（240 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 2.40 Å |
| 1EAI COMPLEX OF ASCARIS CHYMOTRPSIN/ELASTASE INHIBITOR WITH PORCINE ELASTASE Deposited 1999-03-25 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
27–266（240 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 2.40 Å |
| 1EAS NONPEPTIDIC INHIBITORS OF HUMAN LEUKOCYTE ELASTASE. 3. DESIGN, SYNTHESIS, X-RAY CRYSTALLOGRAPHIC ANALYSIS, AND STRUCTURE-ACTIVITY RELATIONSHIPS FOR A SERIES OF ORALLY ACTIVE 3-AMINO-6-PHENYLPYRIDIN-2-ONE TRIFLUOROMETHYL KETONES Deposited 1994-11-22 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 3 TFK 3-[[(METHYLAMINO)SULFONYL]AMINO]-2-OXO-6-PHENYL-N-[3,3,3-TRIFLUORO-1-(1-METHYLETHYL)-2-OXOPHENYL]-1(2H)-PYRIDINE ACETAMIDE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.80 Å |
| 1EAT NONPEPTIDIC INHIBITORS OF HUMAN LEUKOCYTE ELASTASE. 5. DESIGN, SYNTHESIS, AND X-RAY CRYSTALLOGRAPHY OF A SERIES OF ORALLY ACTIVE 5-AMINO-PYRIMIDIN-6-ONE-CONTAINING TRIFLUOROMETHYLKETONES Deposited 1994-11-22 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 2 TFI 2-[5-METHANESULFONYLAMINO-2-(4-AMINOPHENYL)-6-OXO-1,6-DIHYDRO-1-PYRIMIDINYL]-N-(3,3,3-TRIFLUORO-1-ISOPROPYL-2-OXOPROPYL)ACETAMIDE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1EAU NONPEPTIDIC INHIBITORS OF HUMAN LEUKOCYTE ELASTASE. 6. DESIGN OF A POTENT, INTRATRACHEALLY ACTIVE, PYRIDONE-BASED TRIFLUOROMETHYL KETONE Deposited 1994-11-22 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 1 BDK 2-[5-AMINO-6-OXO-2-(2-THIENYL)-1,6-DIHYDROPYRIMIDIN-1-YL)-N-[3,3-DIFLUORO -1-ISOPROPYL-2-OXO-3-(N-(2-MORPHOLINO ETHYL)CARBAMOYL]PROPYL]ACETAMIDE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1ELA Analogous inhibitors of elastase do not always bind analogously Deposited 1993-12-07 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | 0Z1 6-ammonio-N-(trifluoroacetyl)-L-norleucyl-N-[4-(1-methylethyl)phenyl]-L-prolinamide × 1 CA CALCIUM ION × 1 SO4 SULFATE ION × 1 ACY ACETIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
THE COMPLEX WAS CO-CRYSTALLIZED. THE CO-CRYSTALS WERE
GROWN BY THE METHOD OF VAPOR DIFFUSION IN 40 MICROLITER
DROPS CONTAINING 7MG/ML OF ELASTASE, 0.1 MILLIMOLAR
TFA-LYS-PRO-ISO, 1.4 MILLIMOLAR SODIUM SULFATE AND 100
MILLIMOLAR ACETATE BUFFER AT PH 5.
|
Resolution 2.00 Å |
| 1ELB Analogous inhibitors of elastase do not always bind analogously Deposited 1993-12-07 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | 0Z4 6-ammonio-N-(trifluoroacetyl)-L-norleucyl-N-[4-(1-methylethyl)phenyl]-L-leucinamide × 1 CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
THE COMPLEX WAS DONE BY SOAKING OF THE INHIBITOR. THE
NATIVE ELASTASE CRYSTALS WERE TRANSFERRED FROM THE SITTING
DROP TO A HIGH-SALT MOTHER LIQUOR CONTAINING THE
TFA-LYS-PRO-ISO INHIBITOR AT A CONCENTRATION OF 0.1
MILLIMOLAR. THE INHIBITOR WAS SOAKED FOR FOUR DAYS.
|
Resolution 2.10 Å |
| 1ELC Analogous inhibitors of elastase do not always bind analogously Deposited 1993-12-07 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | 0Z3 6-ammonio-N-(trifluoroacetyl)-L-norleucyl-N-[4-(1-methylethyl)phenyl]-L-phenylalaninamide × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
THE COMPLEX WAS CO-CRYSTALLIZED. THE CO-CRYSTALS WERE
GROWN BY THE METHOD OF VAPOR DIFFUSION IN 40 MICROLITER
DROPS CONTAINING 9MG(SLASH)ML OF ELASTASE, 0.1 MILLIMOLAR
TFA-LYS-PRO-ISO, 1.4 MILLIMOLAR SODIUM SULFATE AND 100
MILLIMOLAR SODIUM ACETATE AT PH 5. THE RESERVOIR CONSISTED
OF 100 MILLIMOLAR SODIUM SULFATE AND 100 MILLIMOLAR ACETATE
BUFFER AT PH 5.
|
Resolution 1.75 Å |
| 1ELD Structural analysis of the active site of porcine pancreatic elastase based on the x-ray crystal structures of complexes with trifluoroacetyl-dipeptide-anilide inhibitors Deposited 1994-10-24 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
27–266（240 aa）
|
Not recorded | 0Z0 N-(trifluoroacetyl)-L-phenylalanyl-N-[4-(trifluoromethyl)phenyl]-L-alaninamide × 1 CA CALCIUM ION × 1 ACY ACETIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
THE COMPLEX WAS CO-CRYSTALLIZED. THE CO-CRYSTALS WERE
GROWN BY THE METHOD OF VAPOR DIFFUSION IN 40 MICROLITER
DROPS CONTAINING 7MG/ML OF ELASTASE, 0.1 MILLIMOLAR
TFA-PHE-ALA-TFM, 1.4 MILLIMOLAR SODIUM SULFATE AND 100
MILLIMOLAR SODIUM ACETATE AT PH 5. THE RESERVOIR CONSISTED
OF 100 MILLIMOLAR SODIUM SULFATE AND 100 MILLIMOLAR ACETATE
BUFFER AT PH 5.
|
Resolution 2.00 Å |
| 1ELE STRUCTURAL ANALYSIS OF THE ACTIVE SITE OF PORCINE PANCREATIC ELASTASE BASED ON THE X-RAY CRYSTAL STRUCTURES OF COMPLEXES WITH TRIFLUOROACETYL-DIPEPTIDE-ANILIDE INHIBITORS Deposited 1994-10-24 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
27–266（240 aa）
|
Not recorded | 0QN N-(trifluoroacetyl)-L-valyl-N-[4-(trifluoromethyl)phenyl]-L-alaninamide × 1 CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
THE INHIBITOR WAS SOAKED INTO NATIVE ELASTASE CRYSTALS.
|
Resolution 2.00 Å |
| 1ELF NATURE OF THE INACTIVATION OF ELASTASE BY N-PEPTIDYL-O-AROYL HYDROXYLAMINE AS A FUNCTION OF PH Deposited 1995-03-13 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 2 BAF (TERT-BUTYLOXYCARBONYL)-ALANYL-AMINO ETHYL-FORMAMIDE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.70 Å |
| 1ELG NATURE OF THE INACTIVATION OF ELASTASE BY N-PEPTIDYL-O-AROYL HYDROXYLAMINE AS A FUNCTION OF PH Deposited 1995-03-13 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 BAA (TERT-BUTYLOXYCARBONYL)-ALANYL-ALANYL-AMINE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.65 Å |
| 1ESA DIRECT STRUCTURE OBSERVATION OF AN ACYL-ENZYME INTERMEDIATE IN THE HYDROLYSIS OF AN ESTER SUBSTRATE BY ELASTASE Deposited 1994-02-04 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.65 Å |
| 1ESB DIRECT STRUCTURE OBSERVATION OF AN ACYL-ENZYME INTERMEDIATE IN THE HYDROLYSIS OF AN ESTER SUBSTRATE BY ELASTASE Deposited 1994-02-04 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | BBL N-[(BENZYLOXY)CARBONYL]-L-ALANINE × 1 CA CALCIUM ION × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.30 Å |
| 1EST THE ATOMIC STRUCTURE OF CRYSTALLINE PORCINE PANCREATIC ELASTASE AT 2.5 ANGSTROMS RESOLUTION. COMPARISONS WITH THE STRUCTURE OF ALPHA-CHYMOTRYPSIN Deposited 1976-05-17 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | SO4 SULFATE ION × 1 TSU PARA-TOLUENE SULFONATE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.50 Å |
| 1FLE CRYSTAL STRUCTURE OF ELAFIN COMPLEXED WITH PORCINE PANCREATIC ELASTASE Deposited 1996-07-04 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
27–266（240 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.9；pH 5.9
|
Resolution 1.90 Å |
| 1FZZ THE CRYSTAL STRUCTURE OF THE COMPLEX OF NON-PEPTIDIC INHIBITOR ONO-6818 AND PORCINE PANCREATIC ELASTASE. Deposited 2000-10-04 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | 681 2-(5-AMINO-6-OXO-2-PHENYL-6H-PYRIMIDIN-1-YL)-N-[2-(5-TERT-BUTYL-1,3,4-OXADIAZOL-2-YL)-1-(METHYLETHYL)-2-HYDROXYETHYL]ACETAMIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；Sodium sulfate, Sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 1.86 Å R-free 0.238 |
| 1GVK Porcine pancreatic elastase acyl enzyme at 0.95 A resolution Deposited 2002-02-14 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
27–266（240 aa）
|
Not recorded | SO4 SULFATE ION × 2 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5；293 K；25 MG/ML PPE, 17.5 MG/ML N-AC-NPI- CO2H, 50MM SODIUM ACETATE PH5.0, 25 MM SODIUM SULPHATE, 20 C, PH 5.00
|
Resolution 0.94 Å R-free 0.147 |
| 1GVK Porcine pancreatic elastase acyl enzyme at 0.95 A resolution Deposited 2002-02-14 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
27–266（240 aa）
|
Not recorded | SO4 SULFATE ION × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5；293 K；25 MG/ML PPE, 17.5 MG/ML N-AC-NPI- CO2H, 50MM SODIUM ACETATE PH5.0, 25 MM SODIUM SULPHATE, 20 C, PH 5.00
|
Resolution 0.94 Å R-free 0.147 |
| 1GWA Triiodide derivative of porcine pancreas elastase Deposited 2002-03-13 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 IOD IODIDE ION × 23 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；PROTEIN SOLN: 5% PPE IN 0.1M CH3COONA PH5.0, 0.02% NAN3 RESERVOIR: 10MM CH3COONA, 10MM NASO4 DROP:5UL PROTEIN SOLN + 5UL RESERVOIR SITTING DROP VAPOUR DIFFUSION, pH 5.00
|
Resolution 1.85 Å R-free 0.205 |
| 1H9L PORCINE PANCREATIC ELASTASE COMPLEXED WITH ACETYL-VAL-GLU-PRO-ILE-COOH Deposited 2001-03-13 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5；CRYSTALS PREPARED FROM 25MM SODIUM SULPHATE, 25MM SODIUM ACETATE (PH 5.0), 25 MG/ML PPE AND THEN SOAKED WITH ACETYL-VAL-GLU-PRO-ILE (17 MG/ML) DISSOLVED IN SODIUM ACETATE (PH 5.0, 50MM) AND SODIUM SULPHATE (25 MM)
|
Resolution 1.67 Å R-free 0.199 |
| 1HAX Snapshots of serine protease catalysis: (A) acyl-enzyme intermediate between porcine pancreatic elastase and human beta-casomorphin-7 at pH 5 Deposited 2001-04-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5；25MM SODIUM SULPHATE, 25MM SODIUM ACETATE (PH 5.0), 25 MG/ML PPE AND 25 MG/ML BCM7.
|
Resolution 1.60 Å R-free 0.201 |
| 1HAY Snapshots of serine protease catalysis: (B) acyl-enzyme intermediate between porcine pancreatic elastase and human beta-casomorphin-7 jumped to pH 10 for 10 seconds Deposited 2001-04-10 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 10；CRYSTALLISED FROM 25MM SODIUM SULPHATE, 25MM SODIUM ACETATE (PH 5.0), 25 MG/ML PPE AND 25 MG/ML BCM7.
|
Resolution 1.70 Å R-free 0.214 |
| 1HAZ Snapshots of serine protease catalysis: (C) acyl-enzyme intermediate between porcine pancreatic elastase and human beta-casomorphin-7 jumped to pH 9 for 1 minute Deposited 2001-04-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 9；CRYSTALLISED FROM 25MM SODIUM SULPHATE, 25MM SODIUM ACETATE (PH 5.0), 25 MG/ML PPE AND 25 MG/ML BCM7.
|
Resolution 1.40 Å R-free 0.202 |
| 1HB0 Snapshots of serine protease catalysis: (D) acyl-enzyme intermediate between porcine pancreatic elastase and human beta-casomorphin-7 jumped to pH 10 for 2 minutes Deposited 2001-04-10 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 10；CRYSTALLISED FROM 25MM SODIUM SULPHATE, 25MM SODIUM ACETATE (PH 5.0), 25 MG/ML PPE AND 25 MG/ML BCM7.
|
Resolution 2.05 Å R-free 0.246 |
| 1HV7 PORCINE PANCREATIC ELASTASE COMPLEXED WITH GW311616A Deposited 2001-01-08 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
Fragment：RESIDUES 27-266
|
Not recorded | SO4 SULFATE ION × 1 616 N-[2-(1-FORMYL-2-METHYL-PROPYL)-1-(4-PIPERIDIN-1-YL-BUT-2-ENOYL)-PYRROLIDIN-3-YL]-METHANESULFONAMIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；293 K；PEG4000, ammonium sulphate, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.201 |
| 1INC CRYSTAL STRUCTURES OF THE COMPLEX OF PORCINE PANCREATIC ELASTASE WITH TWO VALINE-DERIVED BENZOXAZINONE INHIBITORS Deposited 1993-03-19 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | SO4 SULFATE ION × 1 CA CALCIUM ION × 1 ICL [1-(3-CHLORO-2-FORMYL-PHENYLCARBAMOYL)-2-METHYL-PROPYL]-CARBAMIC ACID TERT-BUTYL ESTER × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.94 Å |
| 1JIM STEREOSPECIFIC REACTION OF 3-METHOXY-4-CHLORO-7-AMINOISOCOUMARIN WITH CRYSTALLINE PORCINE PANCREATIC ELASTASE Deposited 1993-03-19 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | SO4 SULFATE ION × 1 ICU METHYL(2-ACETOXY-2-(2-CARBOXY-4-AMINO-PHENYL))ACETATE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.31 Å |
| 1L0Z THE STRUCTURE OF PORCINE PANCREATIC ELASTASE COMPLEXED WITH XENON AND BROMIDE, CRYOPROTECTED WITH DRY PARAFFIN OIL Deposited 2002-02-14 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 BR BROMIDE ION × 32 XE XENON × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；10 mM sodium acetate, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.50 Å R-free 0.209 |
| 1L1G The Structure of Porcine Pancreatic Elastase Complexed with Xenon and Bromide, Cryoprotected with Glycerol Deposited 2002-02-16 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | SO4 SULFATE ION × 1 NA SODIUM ION × 1 BR BROMIDE ION × 8 XE XENON × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；10 mM sodium acetate, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.50 Å R-free 0.215 |
| 1LKA Porcine Pancreatic Elastase/Ca-Complex Deposited 2002-04-24 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | ACT ACETATE ION × 1 CL CHLORIDE ION × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.1；293 K；sodium acetate, sodium citrate, calcium chloride, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.216 |
| 1LKB Porcine Pancreatic Elastase/Na-Complex Deposited 2002-04-24 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | SO4 SULFATE ION × 2 CL CHLORIDE ION × 1 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.1；293 K；sodium acetate, sodium sulfate, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.203 |
| 1LVY PORCINE ELASTASE Deposited 1996-07-20 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.5；CRYSTALLIZED IN NA2SO4 SOLUTION, pH 5.5
|
Resolution 1.87 Å R-free 0.229 |
| 1MCV Crystal Structure Analysis of a Hybrid Squash Inhibitor in Complex with Porcine Pancreatic Elastase Deposited 2002-08-06 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；sodium citrate, ammonium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 1.80 Å R-free 0.221 |
| 1MMJ Porcine pancreatic elastase complexed with a potent peptidyl inhibitor, FR136706 Deposited 2002-09-04 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain N
27–266（240 aa）
|
Not recorded | SO4 SULFATE ION × 1 CA CALCIUM ION × 1 FR1 2-[4-[[(S)-1-[[(S)-2-[[(RS)-3,3,3-TRIFLUORO-1-ISOPROPYL-2-OXOPROPYL]AMINOCARBONYL]PYRROLIDIN-1-YL-]CARBONYL]-2-METHYLPROPYL]AMINOCARBONYL]BENZOYLAMINO]ACETIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；sodium sulphate, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 2.20 Å R-free 0.241 |
| 1NES STRUCTURE OF THE PRODUCT COMPLEX OF ACETYL-ALA-PRO-ALA WITH PORCINE PANCREATIC ELASTASE AT 1.65 ANGSTROMS RESOLUTION Deposited 1995-07-31 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain E
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.65 Å |
| 1OKX Binding Structure of Elastase Inhibitor Scyptolin A Deposited 2003-07-31 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
27–266（240 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5；PH 5.00
|
Resolution 2.80 Å R-free 0.260 |
| 1OKX Binding Structure of Elastase Inhibitor Scyptolin A Deposited 2003-07-31 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
27–266（240 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5；PH 5.00
|
Resolution 2.80 Å R-free 0.260 |
| 1QGF PORCINE PANCREATIC ELASTASE COMPLEXED WITH (3R, 4S)N-PARA-TOLUENESULPHONYL-3-ETHYL-4-(CARBOXYLIC ACID)PYRROLIDIN-2-ONE Deposited 1999-04-27 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 TPX (2S,3S)-3-FORMYL-2-({[(4-METHYLPHENYL)SULFONYL]AMINO}METHYL)PENTANOIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5；25MM SODIUM SULPHATE, 25MM SODIUM ACETATE, PH 5.0; THEN SOAKED IN 25 MM SODIUM
SULPHATE, 50MM SODIUM ACETATE, 20 MG/ML GAMMA-LACTAM, 10% (V/V) DMSO, PH 5.0
FOR 24 HOURS, pH 5.00
|
Resolution 1.70 Å R-free 0.210 |
| 1QIX Porcine pancreatic elastase complexed with human beta-casomorphin-7 Deposited 1999-06-18 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5；CRYSTALLISED FROM 25MM SODIUM SULPHATE, 25MM SODIUM ACETATE (PH 5.0), 25 MG/ML PPE AND 25 MG/ML BCM7.
|
Resolution 1.90 Å R-free 0.219 |
| 1QNJ THE STRUCTURE OF NATIVE PORCINE PANCREATIC ELASTASE AT ATOMIC RESOLUTION (1.1 A) Deposited 1999-10-15 | Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；291 K；0.25 M NA2SO4 18 DEGREES CELSIUS, pH 8.00
|
Resolution 1.10 Å R-free 0.165 |
| 1QR3 Structure of porcine pancreatic elastase in complex with FR901277, a novel macrocyclic inhibitor of elastases at 1.6 angstrom resolution Deposited 1999-06-18 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；293 K；SODIUM SULFATE, SODIUM ACETATE, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.60 Å |
| 1UO6 PORCINE PANCREATIC ELASTASE/Xe-COMPLEX Deposited 2003-09-16 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | GOL GLYCEROL × 1 SO4 SULFATE ION × 2 CL CHLORIDE ION × 1 NA SODIUM ION × 1 XE XENON × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.1；SODIUM ACETATE, SODIUM SULFATE, pH 5.10
|
Resolution 1.65 Å R-free 0.208 |
| 1UVO Structure Of The Complex Of Porcine Pancreatic Elastase In Complex With Cadmium Refined At 1.85 A Resolution (Crystal A) Deposited 2004-01-21 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–240（240 aa）
|
Not recorded | ACT ACETATE ION × 1 GOL GLYCEROL × 1 CL CHLORIDE ION × 1 CD CADMIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.1；pH 5.10
|
Resolution 1.85 Å R-free 0.205 |
| 1UVP Structure Of The Complex Of Porcine Pancreatic Elastase In Complex With Cadmium Refined At 1.85 A Resolution (Crystal B) Deposited 2004-01-21 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CD CADMIUM ION × 1 ACT ACETATE ION × 1 GOL GLYCEROL × 1 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.1；SODIUM ACETATE,SODIUM SULFATE, pH 5.10
|
Resolution 1.85 Å R-free 0.205 |
| 2A7C On the Routine Use of Soft X-Rays in Macromolecular Crystallography, Part III- The Optimal Data Collection Wavelength Deposited 2005-07-05 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | SO4 SULFATE ION × 2 XE XENON × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.65 Å R-free 0.182 |
| 2A7J On the Routine Use of Soft X-Rays in Macromolecular Crystallography, Part III- The Optimal Data Collection Wavelength Deposited 2005-07-05 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | ACT ACETATE ION × 1 CA CALCIUM ION × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.65 Å R-free 0.189 |
| 2BB4 Porcine pancreatic elastase complexed with beta-casomorphin-7 and Asp-Phe at pH 5.0 Deposited 2005-10-17 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；294 K；25MM SODIUM SULPHATE, 25MM SODIUM ACETATE (PH 5.0), 25 MG/ML PPE, 17.5 MG/ML BETA-CASOMORPHIN-7, AND THEN SOAKED WITH ASP-PHE (2MG) IN 250MM SODIUM ACETATE (PH 5.0) (5 MICROLITRES) FOR 30 MIN, VAPOR DIFFUSION, HANGING DROP, temperature 294K
|
Resolution 1.60 Å R-free 0.197 |
| 2BD2 Porcine pancreatic elastase complexed with beta-casomorphin-7 and Arg-Phe at pH 5.0 Deposited 2005-10-20 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；294 K；25MM SODIUM SULPHATE, 25MM SODIUM ACETATE (PH 5.0), 25 MG/ML PPE AND 17.5 MG/ML BCM7, AND THEN SOAKED WITH ARG-PHE (2MG) IN 250MM SODIUM ACETATE (PH 5.0) (5 MICROLITRES) FOR 45 MIN, VAPOR DIFFUSION, HANGING DROP, temperature 294K
|
Resolution 1.70 Å R-free 0.205 |
| 2BD3 Porcine pancreatic elastase complexed with beta-casomorphin-7 and Lys-Ala-NH2 at pH 5.0 Deposited 2005-10-20 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；294 K；25MM SODIUM SULPHATE, 25MM SODIUM ACETATE (PH 5.0), 25 MG/ML PPE AND 17.5 MG/ML BCM7, AND THEN SOAKED WITH Lys-Ala-NH2 (50MG/ML) IN 250MM SODIUM ACETATE (PH 5.0) (5 MICROLITRES) FOR 30 MIN, VAPOR DIFFUSION, HANGING DROP, temperature 294K
|
Resolution 1.60 Å R-free 0.201 |
| 2BD4 Porcine pancreatic elastase complexed with beta-casomorphin-7 and Lys-Ser at pH 5.0 Deposited 2005-10-20 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；294 K；25MM SODIUM SULPHATE, 25MM SODIUM ACETATE (PH 5.0), 25 MG/ML PPE AND 17.5 MG/ML BCM7, AND THEN SOAKED WITH LYS-SER (50MG/ML) IN 250MM SODIUM ACETATE (PH 5.0) (5 MICROLITRES) FOR 60 MIN, VAPOR DIFFUSION, HANGING DROP, temperature 294K
|
Resolution 1.70 Å R-free 0.220 |
| 2BD5 Porcine pancreatic elastase complexed with beta-casomorphin-7 and Lys-Ser at pH 5 and immersed in pH 9 buffer for 30 seconds Deposited 2005-10-20 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；294 K；25MM SODIUM SULPHATE, 25MM SODIUM ACETATE (PH 5.0), 25 MG/ML PPE AND 17.5 MG/ML BCM7, AND THEN SOAKED WITH LYS-SER (50 MG/ML) IN 250MM SODIUM ACETATE (PH 5.0) (5 MICROLITRES) FOR 60 MIN , VAPOR DIFFUSION, HANGING DROP, temperature 294K
|
Resolution 1.80 Å R-free 0.212 |
| 2BD7 Porcine pancreatic elastase complexed with beta-casomorphin-7 and Arg-Phe at pH 5.0 (50 min soak) Deposited 2005-10-20 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；294 K；25MM SODIUM SULPHATE, 25MM SODIUM ACETATE (PH 5.0), 25 MG/ML PPE AND 17.5 MG/ML BCM7, AND THEN SOAKED WITH ARG-PHE (2 MG) IN 250MM SODIUM ACETATE (PH 5.0) (5 MICROLITRES) FOR 50 MIN, VAPOR DIFFUSION, HANGING DROP, temperature 294K
|
Resolution 1.60 Å R-free 0.211 |
| 2BD8 Porcine pancreatic elastase complexed with beta-casomorphin-7 and Arg-Phe at pH 5.0 (50 min soak) and immersed in pH 9 buffer for 30 seconds Deposited 2005-10-20 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；294 K；25MM SODIUM SULPHATE, 25MM SODIUM ACETATE (PH 5.0), 25 MG/ML PPE AND 17.5 MG/ML BCM7, AND THEN SOAKED WITH ARG-PHE (2 MG) IN 250MM SODIUM ACETATE (PH 5.0) (5 MICROLITRES) FOR 50 MIN, VAPOR DIFFUSION, HANGING DROP, temperature 294K
|
Resolution 1.70 Å R-free 0.220 |
| 2BD9 Porcine pancreatic elastase complexed with beta-casomorphin-7 and Arg-Phe at pH 5.0 (50 min soak) and immersed in pH 9 buffer for 28 seconds (2nd pH jump) Deposited 2005-10-20 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；294 K；25MM SODIUM SULPHATE, 25MM SODIUM ACETATE (PH 5.0), 25 MG/ML PPE AND 17.5 MG/ML BCM7, AND THEN SOAKED WITH ARG-PHE (2 MG) IN 250MM SODIUM ACETATE (PH 5.0) (5 MICROLITRES) FOR 50 MIN , VAPOR DIFFUSION, HANGING DROP, temperature 294K
|
Resolution 1.90 Å R-free 0.226 |
| 2BDA Porcine pancreatic elastase complexed with N-acetyl-NPI and Ala-Ala at pH 5.0 Deposited 2005-10-20 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；294 K；25MM SODIUM SULPHATE, 25MM SODIUM ACETATE (PH 5.0), 25 MG/ML PPE AND 17.5 MG/ML AcNPI, AND THEN SOAKED WITH ALA-ALA (2 MG) IN 250MM SODIUM ACETATE (PH 5.0) (5 MICROLITRES) FOR 30 MIN, VAPOR DIFFUSION, HANGING DROP, temperature 294K
|
Resolution 1.80 Å R-free 0.212 |
| 2BDB Porcine pancreatic elastase complexed with Asn-Pro-Ile and Ala-Ala at pH 5.0 Deposited 2005-10-20 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 ALA ALANINE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；294 K；25MM SODIUM SULPHATE, 25MM SODIUM ACETATE (PH 5.0), 25 MG/ML PPE AND 17.5 MG/ML NPI, AND THEN SOAKED WITH Ala-Ala (2 MG) IN 250MM SODIUM ACETATE (PH 5.0) (5 MICROLITRES) FOR 30 MIN, VAPOR DIFFUSION, HANGING DROP, temperature 294K
|
Resolution 1.70 Å R-free 0.194 |
| 2BDC Porcine pancreatic elastase complexed with Asn-Pro-Ile at pH 5.0 Deposited 2005-10-20 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；294 K；25MM SODIUM SULPHATE, 25MM SODIUM ACETATE (PH 5.0), 25 MG/ML PPE AND 17.5 MG/ML NPI, VAPOR DIFFUSION, HANGING DROP, temperature 294K
|
Resolution 1.80 Å R-free 0.222 |
| 2BLO Elastase before a high dose x-ray "burn" Deposited 2005-03-08 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5；.250 M AMMONIUM SULFATE, .1 M PH 5.0 SODIUM ACETATE
|
Resolution 1.33 Å R-free 0.140 |
| 2BLQ Elastase After A High Dose X-Ray "Burn" Deposited 2005-03-08 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
.250 M AMMONIUM SULFATE,.1 M PH 5.0 SODIUM ACETATE
|
Resolution 1.33 Å R-free 0.142 |
| 2CV3 Crystal structure of porcine pancreatic elastase complexed with a macroclyclic peptide inhibitor Deposited 2005-05-31 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
27–266（240 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；293 K；Sodium sulfate, sodium aceteate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.90 Å R-free 0.224 |
| 2D26 Active site distortion is sufficient for proteinase inhibit second crystal structure of covalent serpin-proteinase complex Deposited 2005-09-03 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
27–266（240 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；291 K；PEG3350, pH 8.10, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 3.30 Å R-free 0.312 |
| 2DE8 Crystal structure of porcine pancreatic elastase with a unique conformation induced by Tris Deposited 2006-02-09 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CL CHLORIDE ION × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；277 K；0.3M sodium chloride, 50mM Tris buffer, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.50 Å R-free 0.246 |
| 2DE9 Crystal structure of porcine pancreatic elastase complexed with Tris after soaking a tris-free solution Deposited 2006-02-09 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；277 K；0.3M sodium chloride, 50mM Tris , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.30 Å R-free 0.226 |
| 2EST Crystallographic study of the binding of a trifluoroacetyl dipeptide anilide inhibitor with elastase Deposited 1986-03-24 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
27–266（240 aa）
|
Not recorded | 2Z5 6-ammonio-N-(trifluoroacetyl)-L-norleucyl-N-[4-(trifluoromethyl)phenyl]-L-alaninamide × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.50 Å |
| 2FO9 Structure of porcine pancreatic elastase in 95% acetone Deposited 2006-01-13 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 2 ACN ACETONE × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；291 K；15 mg/ml elastase, 25 mM Sodium Acetate, 135 mM Sodium sulfate, 10 ul drop (5x5). See also: Sawyer et al., J.Mol.Biol.118, 137-208 (1978)., pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.00 Å R-free 0.230 |
| 2FOA Structure of porcine pancreatic elastase in 40/50/10 % benzene Deposited 2006-01-13 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；291 K；15 mg/ml elastase, 25 mM Sodium Acetate, 135 mM Sodium sulfate, 10 ul drop (5x5). See also: Sawyer et al., J.Mol.Biol. 118, 137-208 (1978)., pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 1.90 Å R-free 0.200 |
| 2FOB Structure of porcine pancreatic elastase in 40/50/10 cyclohexane Deposited 2006-01-13 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 IPA ISOPROPYL ALCOHOL × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；291 K；15 mg/ml elastase, 25 mM Sodium Acetate, 135 mM Sodium sulfate, 10 ul drop (5x5). See also: Sawyer et al., J.Mol.Biol. 118, 137-208 (1978)., pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 1.90 Å R-free 0.198 |
| 2FOC Structure of porcine pancreatic elastase in 55% dimethylformamide Deposited 2006-01-13 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 2 DMF DIMETHYLFORMAMIDE × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；291 K；15 mg/ml elastase, 25 mM Sodium Acetate, 135 mM Sodium sulfate, 10 ul drop (5x5). See also: Sawyer et al., J.Mol.Biol. 118, 137-208 (1978). , pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.00 Å R-free 0.234 |
| 2FOD Structure of porcine pancreatic elastase in 80% ethanol Deposited 2006-01-13 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 EOH ETHANOL × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；291 K；15 mg/ml elastase, 25 mM Sodium Acetate, 135 mM Sodium sulfate, 10 ul drop (5x5). See also: Sawyer et al., J.Mol.Biol. 118, 37-208 (1978)., pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.00 Å R-free 0.224 |
| 2FOE Structure of porcine pancreatic elastase in 80% hexane Deposited 2006-01-13 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 2 217 (2S)-HEX-5-ENE-1,2-DIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；291 K；15 mg/ml elastase, 25 mM Sodium Acetate, 135 mM Sodium sulfate, 10 ul drop (5x5). See also: Sawyer et al., J.Mol.Biol. 118, 137-208 (1978). , pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.20 Å R-free 0.205 |
| 2FOF Structure of porcine pancreatic elastase in 80% isopropanol Deposited 2006-01-13 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；291 K；15 mg/ml elastase, 25 mM Sodium Acetate, 135 mM Sodium sulfate, 10 ul drop (5x5). See also: Sawyer et al., J.Mol.Biol. 118, 137-208 (1978), pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.20 Å R-free 0.199 |
| 2FOG Structure of porcine pancreatic elastase in 40% trifluoroethanol Deposited 2006-01-13 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 ETF TRIFLUOROETHANOL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；291 K；15 mg/ml elastase, 25 mM Sodium Acetate, 135 mM Sodium sulfate, 10 ul drop (5x5). See also: Sawyer et al., J.Mol.Biol. 118, 137-208 (1978), pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 1.90 Å R-free 0.220 |
| 2FOH Structure of porcine pancreatic elastase in 40% trifluoroethanol Deposited 2006-01-13 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 ETF TRIFLUOROETHANOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；291 K；15 mg/ml elastase, 25 mM Sodium Acetate, 135 mM Sodium sulfate, 10 ul drop (5x5). See also: Sawyer et al., J.Mol.Biol. 118, 137-208 (1978), pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 1.80 Å R-free 0.193 |
| 2G4T anomalous substructure of porcine pancreatic elastase (Na) Deposited 2006-02-22 | Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.15 Å R-free 0.239 |
| 2G4U Anomalous substructure of porcine pancreatic elastaase (Ca) Deposited 2006-02-22 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 FLC CITRATE ANION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.84 Å R-free 0.177 |
| 2H1U Porcine pancreatic elastase complexed with MetPheLeuGlu at pH 5.0 Deposited 2006-05-17 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；294 K；25mm sodium sulphate, 25mm sodium acetate (ph 5.0), 25mg/ml PPE, 17.5mg/ml MFLE , VAPOR DIFFUSION, HANGING DROP, temperature 294K
|
Resolution 1.60 Å R-free 0.205 |
| 2IOT Clavulanic Acid bound to Elastase Deposited 2006-10-10 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | SO4 SULFATE ION × 1 CL5 N-(3-OXOPROPYL)GLYCINE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；200uM Na2SO4, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.60 Å R-free 0.235 |
| 2OQU High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths Deposited 2007-02-01 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 XE XENON × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；30mM sodium sulfate, 50mM sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.226 |
| 2V0B SAD Structure solution porcine pancreatic elastase from a Selenate derivative Deposited 2007-05-11 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SE4 SELENATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.050 M NASEO4, 20 % ETHYLENE GLYCOL, USING THE HANGING DROP METHOD AT 20 C., pH 7.0
|
Resolution 1.65 Å R-free 0.206 |
| 2V35 Porcine Pancreatic Elastase in complex with inhibitor JM54 Deposited 2007-06-12 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | J54 (2R)-3-{[(BENZYLAMINO)CARBONYL]AMINO}-2-HYDROXYPROPANOIC ACID × 1 NA SODIUM ION × 1 SO4 SULFATE ION × 1 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.1；100 MM SODIUM ACETATE PH 5.1 200 MM SODIUM SULFATE
|
Resolution 1.67 Å R-free 0.186 |
| 3E3T Structure of porcine pancreatic elastase with the magic triangle I3C Deposited 2008-08-08 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | SO4 SULFATE ION × 2 NA SODIUM ION × 1 IOD IODIDE ION × 1 I3C 5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；277 K；0.1M HEPES, 0.6M sodium sulfate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.60 Å R-free 0.213 |
| 3EST STRUCTURE OF NATIVE PORCINE PANCREATIC ELASTASE AT 1.65 ANGSTROMS RESOLUTION Deposited 1987-09-17 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.65 Å |
| 3HGN Structure of porcine pancreatic elastase complexed with a potent peptidyl inhibitor FR130180 determined by neutron crystallography Deposited 2009-05-14 | Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | FRW 4-[[(2S)-3-methyl-1-oxo-1-[(2S)-2-[[(3S)-1,1,1-trifluoro-4-methyl-2-oxo-pentan-3-yl]carbamoyl]pyrrolidin-1-yl]butan-2-yl]carbamoyl]benzoic acid × 1 CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
Experimental method not declared
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；293 K；0.3M sodium sulphate, 0.05M D-substituted sodium acetate, pH5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.65 Å |
| 3HGP Structure of porcine pancreatic elastase complexed with a potent peptidyl inhibitor FR130180 determined by high resolution crystallography Deposited 2009-05-14 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | FRW 4-[[(2S)-3-methyl-1-oxo-1-[(2S)-2-[[(3S)-1,1,1-trifluoro-4-methyl-2-oxo-pentan-3-yl]carbamoyl]pyrrolidin-1-yl]butan-2-yl]carbamoyl]benzoic acid × 1 CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；0.3M sodium sulphate, 0.05M sodium acetate, pH5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 0.94 Å R-free 0.128 |
| 3MNB Investigation of global and local effects of radiation damage on porcine pancreatic elastase. First stage of radiation damage Deposited 2010-04-21 | Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.2；297 K；The initial concentration of the protein was 10 mg/ml in water. The reservoir contained 25 mM Na2SO4. The cryo-solution contained 25 mM Na2SO4 and 25% glycerol, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
Resolution 1.20 Å R-free 0.138 |
| 3MNC Investigation of global and local effects of radiation damage on porcine pancreatic elastase. Second stage of radiation damage Deposited 2010-04-21 | Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.2；297 K；The initial concentration of the protein was 10 mg/ml in water. The reservoir contained 25 mM Na2SO4. The cryo-solution contained 25 mM Na2SO4 and 25% glycerol, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
Resolution 1.12 Å R-free 0.141 |
| 3MNS Investigation of global and local effects of radiation damage on porcine pancreatic elastase. Third stage of radiation damage Deposited 2010-04-22 | Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.2；297 K；The initial concentration of the protein was 10 mg/ml in water. The reservoir contained 25 mM Na2SO4, pH = 8.2. The cryo-solution contained 25 mM Na2SO4 and 25% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
Resolution 1.50 Å R-free 0.168 |
| 3MNX Investigation of global and local effects of radiation damage on porcine pancreatic elastase. Fourth stage of radiation damage Deposited 2010-04-22 | Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.2；297 K；The initial concentration of the protein was 10 mg/ml in water. The reservoir contained 25 mM Na2SO4, pH = 8.2. The cryo-solution contained 25 mM Na2SO4 and 25% glycerol , VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
Resolution 1.39 Å R-free 0.158 |
| 3MO3 Investigation of global and local effects of radiation damage on porcine pancreatic elastase. Fifth stage of radiation damage Deposited 2010-04-22 | Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.2；297 K；The initial concentration of the protein was 10 mg/ml in water. The reservoir contained 25 mM Na2SO4. The cryo-solution contained 25 mM Na2SO4 and 25% glycerol, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
Resolution 1.80 Å R-free 0.211 |
| 3MO6 Investigation of global and local effects of radiation damage on porcine pancreatic elastase. Sixth stage of radiation damage Deposited 2010-04-22 | Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.2；297 K；The initial concentration of the protein was 10 mg/ml in water. The reservoir contained 25 mM Na2SO4. The cryo-solution contained 25 mM Na2SO4 and 25% glycerol, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
Resolution 1.66 Å R-free 0.213 |
| 3MO9 Investigation of global and local effects of radiation damage on porcine pancreatic elastase. Seventh stage of radiation damage Deposited 2010-04-22 | Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.2；297 K；The initial concentration of the protein was 10 mg/ml in water. The reservoir contained 25 mM Na2SO4. The cryo-solution contained 25 mM Na2SO4 and 25% glycerol, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
Resolution 2.00 Å R-free 0.233 |
| 3MOC Investigation of global and local effects of radiation damage on porcine pancreatic elastase. Eighth stage of radiation damage Deposited 2010-04-22 | Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.2；297 K；The initial concentration of the protein was 10 mg/ml in water. The reservoir contained 25 mM Na2SO4. The cryo-solution contained 25 mM Na2SO4 and 25% glycerol, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
Resolution 1.82 Å R-free 0.241 |
| 3MTY Comparison of the character and the speed of X-ray-induced structural changes of porcine pancreatic elastase at two temperatures, 100 and 15K. The data set was collected from region A of the crystal. First step of radiation damage Deposited 2010-05-01 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291 K；The initial concentration of the protein was 20 mg/ml in 10% glycerol solution. The reservoir contained a 250 mM Na2SO4. For cryo-protection, it was supplemented with 25% glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 1.10 Å R-free 0.132 |
| 3MU0 Comparison of the character and the speed of X-ray-induced structural changes of porcine pancreatic elastase at two temperatures, 100 and 15K. The data set was collected from region A of the crystal. Third step of radiation damage Deposited 2010-05-01 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291 K；The initial concentration of the protein was 20 mg/ml in 10% glycerol solution. The reservoir contained a 250 mM Na2SO4. For cryo-protection, it was supplemented with 25% glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 1.40 Å R-free 0.142 |
| 3MU1 Comparison of the character and the speed of X-ray-induced structural changes of porcine pancreatic elastase at two temperatures, 100 and 15K. The data set was collected from region A of the crystal. Fifth step of radiation damage Deposited 2010-05-01 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291 K；The initial concentration of the protein was 20 mg/ml in 10% glycerol solution. The reservoir contained a 250 mM Na2SO4. For cryo-protection, it was supplemented with 25% glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 1.74 Å R-free 0.203 |
| 3MU4 Comparison of the character and the speed of X-ray-induced structural changes of porcine pancreatic elastase at two temperatures, 100 and 15K. The data set was collected from region B of the crystal. First step of radiation damage Deposited 2010-05-01 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291 K；The initial concentration of the protein was 20 mg/ml in 10% glycerol solution. The reservoir contained a 250 mM Na2SO4. For cryo-protection, it was supplemented with 25% glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 1.10 Å R-free 0.127 |
| 3MU8 Comparison of the character and the speed of X-ray-induced structural changes of porcine pancreatic elastase at two temperatures, 100 and 15K. The data set was collected from region B of the crystal. Fifth step of radiation damage Deposited 2010-05-02 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291 K；The initial concentration of the protein was 20 mg/ml in 10% glycerol solution. The reservoir contained a 250 mM Na2SO4. For cryo-protection, it was supplemented with 25% glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 1.55 Å R-free 0.176 |
| 3ODD Comparison of the character and the speed of X-ray-induced structural changes of porcine pancreatic elastase at two temperatures, 100 and 15K. The data set was collected from region B of the crystal. Second step of radiation damage Deposited 2010-08-11 | Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；The initial concentration of the protein was 20 mg/ml in 10% glycerol solution. The reservoir contained a 250 mM Na2SO4, solution at pH 7.5 (adjusted with NaOH). For cryo-protection, the 250 mM Na2SO4 was supplemented with 25% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 1.10 Å R-free 0.138 |
| 3ODF Comparison of the character and the speed of X-ray-induced structural changes of porcine pancreatic elastase at two temperatures, 100 and 15K. The data set was collected from region A of the crystal. Second step of radiation damage Deposited 2010-08-11 | Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；The initial concentration of the protein was 20 mg/ml in 10% glycerol solution. The reservoir contained a 250 mM Na2SO4, solution at pH 7.5 (adjusted with NaOH). For cryo-protection, the 250 mM Na2SO4 was supplemented with 25% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 1.10 Å R-free 0.147 |
| 3UOU Crystal structure of the Kunitz-type protease inhibitor ShPI-1 Lys13Leu mutant in complex with pancreatic elastase Deposited 2011-11-17 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
27–266（240 aa）
Fragment：Peptidase S1 domain
|
Not recorded | SO4 SULFATE ION × 8 GOL GLYCEROL × 7 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.6；288 K；0.425M ammonium sulfate, 0.085M tri-sodium citrate, 0.85M Lithium sulfate, 15% glycerol, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 288K
|
Resolution 2.00 Å R-free 0.211 |
| 4EST CRYSTAL STRUCTURE OF THE COVALENT COMPLEX FORMED BY A PEPTIDYL ALPHA,ALPHA-DIFLUORO-BETA-KETO AMIDE WITH PORCINE PANCREATIC ELASTASE AT 1.78-ANGSTROMS RESOLUTION Deposited 1989-05-15 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
27–266（240 aa）
|
Not recorded | SO4 SULFATE ION × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.78 Å |
| 4GVU Lyngbyastatin 7-Porcine Pancreatic Elastase Co-crystal Structure Deposited 2012-08-31 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；0.01 M sodium acetate pH 5.0, 0.42 M sodium sulfate, hanging drop, lyngbyastatin 7 dissolved in methanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.55 Å R-free 0.206 |
| 4YM9 Crystal structure of Porcine Pancreatic Elastase (PPE) in complex with the novel inhibitor JM102 Deposited 2015-03-06 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | 4E4 2-ethyl-2-(hydroxymethyl)-N-(6-methylpyridin-3-yl)butanamide × 1 SO4 SULFATE ION × 5 ACT ACETATE ION × 5 NA SODIUM ION × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.1；295.15 K；200 mM Na2SO4
100 mM NaCH3COO
|
Resolution 1.80 Å R-free 0.157 |
| 5AVD The 0.86 angstrom structure of elastase crystallized in high-strength agarose hydrogel Deposited 2015-06-15 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 5；293 K；0.1 M Na acetate (pH 5.0), 0.03 M (NH4)2SO4
|
Resolution 0.86 Å R-free 0.140 |
| 5EST Crystallographic analysis of the inhibition of porcine pancreatic elastase by a peptidyl boronic acid: structure of a reaction intermediate Deposited 1989-05-15 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
27–266（240 aa）
|
Not recorded | 0P2 N~2~-[(benzyloxy)carbonyl]-N-[(1R,2S)-1-(dihydroxyboranyl)-2-methylbutyl]-L-alaninamide × 1 SO4 SULFATE ION × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.09 Å |
| 6EST INTERACTION OF THE PEPTIDE CF3-LEU-ALA-NH-C6H4-CF3(TFLA) WITH PORCINE PANCREATIC ELASTASE. X-RAY STUDIES AT 1.8 ANGSTROMS Deposited 1990-06-15 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 DMF DIMETHYLFORMAMIDE × 3 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.80 Å |
| 6Q8S Elastase (PPE) under 2 kbar of argon Deposited 2018-12-16 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | NA SODIUM ION × 1 SO4 SULFATE ION × 2 AR ARGON × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 5.5；298 K；20 MICROLITER OF PROTEIN (20 mg/ML) BUFFER ACETATE 0.05M + 2 MICROLITER SODIUM SULFATE SATURATED.
|
Resolution 1.80 Å R-free 0.200 |
| 6QBU Crystal structure of Porcine Pancreatic Elastase (PPE) in complex with the 3-Oxo-beta-Sultam inhibitor LMC188 Deposited 2018-12-21 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | HVZ ~{N}-[[1-[(4-chlorophenyl)methyl]-1,2,3-triazol-4-yl]methyl]-2-hydrosulfonyl-2-methyl-propanamide × 1 PO4 PHOSPHATE ION × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.9；293.15 K；70% (V/V) MPD
10 mM KPi buffer pH 5.9
|
Resolution 1.38 Å R-free 0.163 |
| 6QEN Crystal structure of Porcine Pancreatic Elastase (PPE) in complex with the 3-Oxo-beta-Sultam inhibitor LMC240 Deposited 2019-01-08 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | J05 1-[[1-[(4-bromophenyl)methyl]-1,2,3-triazol-4-yl]methylamino]-2-methyl-1-oxidanylidene-propane-2-sulfonic acid × 1 PO4 PHOSPHATE ION × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.9；293.15 K；70% (V/V) MPD
10 mM KP Buffer pH 5.9
|
Resolution 1.20 Å R-free 0.174 |
| 6QEO Crystal structure of Porcine Pancreatic Elastase (PPE) in complex with the 3-Oxo-beta-Sultam inhibitor LMC269 Deposited 2019-01-08 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | J02 2-methyl-1-[[1-[(4-nitrophenyl)methyl]-1,2,3-triazol-4-yl]methylamino]-1-oxidanylidene-propane-2-sulfonic acid × 1 PO4 PHOSPHATE ION × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.9；293.15 K；70 % (V/V) MPD
10 mM KP pH 5.9
|
Resolution 1.30 Å R-free 0.167 |
| 6TH7 Structure of porcine pancreatic elastase in complex with tutuilamide Deposited 2019-11-18 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；0sodium acetate, tri-sodium citrate, ammonium sulfate
|
Resolution 2.20 Å R-free 0.244 |
| 6TH7 Structure of porcine pancreatic elastase in complex with tutuilamide Deposited 2019-11-18 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；0sodium acetate, tri-sodium citrate, ammonium sulfate
|
Resolution 2.20 Å R-free 0.244 |
| 7EST Interaction of the peptide CF3-LEU-ALA-NH-C6H4-CF3(TFLA) with porcine pancreatic elastase. X-ray studies at 1.8 Angstroms Deposited 1990-06-15 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
27–266（240 aa）
|
Not recorded | 0Z2 N-(trifluoroacetyl)-L-leucyl-N-[4-(trifluoromethyl)phenyl]-L-alaninamide × 1 SO4 SULFATE ION × 1 CA CALCIUM ION × 1 DMF DIMETHYLFORMAMIDE × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.7；10% DMF SOLUTION BUFFERED AT PH 5.7 WITH ACETATE
|
Resolution 1.80 Å |
| 7FAG Room temperature structure of elastase with high-strength agarose hydrogel Deposited 2021-07-06 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 SO3 SULFITE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；277 K；0.1M Na acetate (pH8.5), 0.03M ammonium sulfate
|
Resolution 1.30 Å R-free 0.153 |
| 8B04 STRUCTURE OF PORCINE PANCREATIC ELASTASE BOUND TO A FRAGMENT OF AN ISOXAZOLONE INHIBITOR Deposited 2022-09-07 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–266（266 aa）
|
Not recorded | DMS DIMETHYL SULFOXIDE × 2 OU0 2-cyclopropylcarbonyl-3-propan-2-yl-1,2-oxazol-5-one × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.1；296 K；sodium sulphate, sodium acetate
|
Resolution 1.60 Å R-free 0.188 |
| 8B1Y STRUCTURE OF PORCINE PANCREATIC ELASTASE BOUND TO A FRAGMENT OF A 5-AZAINDAZOLE INHIBITOR Deposited 2022-09-12 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–266（266 aa）
|
Not recorded | OTO 1-cyclopropylcarbonylpyrazolo[4,3-c]pyridine-3-carbonitrile × 1 GOL GLYCEROL × 1 SO4 SULFATE ION × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.1；296 K；SODIUM SULFATE, SODIUM ACETATE
|
Resolution 1.12 Å R-free 0.150 |
| 8B49 STRUCTURE OF PORCINE PANCREATIC ELASTASE BOUND TO A FRAGMENT (m-toluoylcarbonyl group) OF A 5-AZAINDOLE INHIBITOR Deposited 2022-09-20 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–266（266 aa）
|
Not recorded | OW9 1-(3-methylphenyl)carbonylpyrrolo[3,2-c]pyridine-3-carbonitrile × 1 GOL GLYCEROL × 1 CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.1；296 K；sodium sulfate, sodium acetate
|
Resolution 1.19 Å R-free 0.143 |
| 8B53 Structure of porcine pancreatic elastase bound to a fragment of a 4-azaindole inhibitor Deposited 2022-09-21 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–266（266 aa）
|
Not recorded | OVV 3-methylbenzoic acid × 1 SO4 SULFATE ION × 1 GOL GLYCEROL × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.1；296 K；sodium sulfate, sodium acetate
|
Resolution 1.25 Å R-free 0.135 |
| 8EST REACTION OF PORCINE PANCREATIC ELASTASE WITH 7-SUBSTITUTED 3-ALKOXY-4-CHLOROISOCOUMARINS: DESIGN OF POTENT INHIBITORS USING THE CRYSTAL STRUCTURE OF THE COMPLEX FORMED WITH 4-CHLORO-3-ETHOXY-7-GUANIDINO-ISOCOUMARIN Deposited 1990-02-21 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
27–266（240 aa）
|
Not recorded | SO4 SULFATE ION × 1 CA CALCIUM ION × 1 GIS ETHYL-(2-CARBOXY-4-GUANIDINIUM-PHENYL)-CHLOROACETATE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.78 Å |
| 9EST STRUCTURAL STUDY OF PORCINE PANCREATIC ELASTASE COMPLEXED WITH 7-AMINO-3-(2-BROMOETHOXY)-4-CHLOROISOCOUMARIN AS A NONREACTIVATABLE DOUBLY COVALENT ENZYME-INHIBITOR COMPLEX Deposited 1991-01-14 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
27–266（240 aa）
|
Not recorded | SO4 SULFATE ION × 1 CA CALCIUM ION × 1 IBR (2-BROMOETHYL)(2-'FORMYL-4'-AMINOPHENYL) ACETATE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å |
126 other PDB entries and 131 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | CELA1_PIG |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–240； UniProt 27–266 |
