← Back to search
3th0

P22 Tailspike complexed with S.Paratyphi O antigen octasaccharide

Method: X-RAY DIFFRACTION Dmax: 128.4 Å Quality: REASONABLE
▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

Bifunctional tail protein

Enterobacteria phage P22

UniProt P12528

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Homooligomer Protein × 3 其他Polymer 3 PDB declaration: trimeric（3） Consistent with protein copy count Chain A； UniProt 109–667 Fragment：UNP residues 109-657 ;alpha-D-galactopyranose-(1-2)-[alpha-D-Paratopyranose-(1-3)]alpha-D-mannopyranose-(1-4)-alpha-L-rhamnopyranose-(1-3)-alpha-D-galactopyranose-(1-2)-[alpha-D-Paratopyranose-(1-3)]alpha-D-mannopyranose-(1-4)-alpha-L-rhamnopyranose ; × 3 GOL GLYCEROL × 9 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 10；277.15 K；1.5 Ammonium sulfate, 0.1M sodium phosphate over reservoir 1.0 Ammonium sulfate, 0.1M sodium phosphate, pH 10, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K X-ray crystallization conditions：MICRODIALYSIS；pH 7.5；293.15 K；addition of 2mM S.Paratyphi o antigen octasaccharide in 0.1M Tris, 1M sodium phosphate, pH 7.5, MICRODIALYSIS, temperature 293.15K Resolution 1.75 Å R-free 0.156

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 3TH0 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1CLW TAILSPIKE PROTEIN FROM PHAGE P22, V331A MUTANT Deposited 1999-05-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 114–667（554 aa） Fragment：CATALYTIC FRAGMENT
Mutation：V331A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 10；pH 10.00
Resolution 2.00 Å
1CLW TAILSPIKE PROTEIN FROM PHAGE P22, V331A MUTANT Deposited 1999-05-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 114–667（554 aa） Fragment：CATALYTIC FRAGMENT
Mutation：V331A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 10；pH 10.00
Resolution 2.00 Å
1LKT CRYSTAL STRUCTURE OF THE HEAD-BINDING DOMAIN OF PHAGE P22 TAILSPIKE PROTEIN Deposited 1997-10-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 6–109（104 aa） Fragment：HEAD-BINDING DOMAIN
Chain B 6–109（104 aa） Fragment：HEAD-BINDING DOMAIN
Chain C 6–109（104 aa） Fragment：HEAD-BINDING DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.6；20% PEG 8K, 0.2 M MGCL2, 0.1 M BIS-TRIS, PH 6.6
Resolution 2.60 Å
1LKT CRYSTAL STRUCTURE OF THE HEAD-BINDING DOMAIN OF PHAGE P22 TAILSPIKE PROTEIN Deposited 1997-10-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain D 6–109（104 aa） Fragment：HEAD-BINDING DOMAIN
Chain E 6–109（104 aa） Fragment：HEAD-BINDING DOMAIN
Chain F 6–109（104 aa） Fragment：HEAD-BINDING DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.6；20% PEG 8K, 0.2 M MGCL2, 0.1 M BIS-TRIS, PH 6.6
Resolution 2.60 Å
1QA1 TAILSPIKE PROTEIN, MUTANT V331G Deposited 1999-04-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 114–667（554 aa） Fragment：RECEPTOR BINDING C-TERMINAL DOMAIN
Mutation：V331G No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 10；274 K；VAPOR DIFFUSION, HANGING DROP, 274 K, PH 10.0, 1M AMMONIUM SULPHATE 0.1M NA- PHOSPHATE
Resolution 2.00 Å
1QA1 TAILSPIKE PROTEIN, MUTANT V331G Deposited 1999-04-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 114–667（554 aa） Fragment：RECEPTOR BINDING C-TERMINAL DOMAIN
Mutation：V331G No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 10；274 K；VAPOR DIFFUSION, HANGING DROP, 274 K, PH 10.0, 1M AMMONIUM SULPHATE 0.1M NA- PHOSPHATE
Resolution 2.00 Å
1QA2 TAILSPIKE PROTEIN, MUTANT A334V Deposited 1999-04-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 114–667（554 aa） Fragment：RECEPTOR BINDING C-TERMINAL FRAGMENT
Mutation：A334V No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 10；277 K；VAPOR DIFFUSION, HANGING DROP, 277K, PH 10.0, 1M AMMONIUM SULPHATE 0.1M NA- PHOSPHATE
Resolution 2.00 Å
1QA2 TAILSPIKE PROTEIN, MUTANT A334V Deposited 1999-04-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 114–667（554 aa） Fragment：RECEPTOR BINDING C-TERMINAL FRAGMENT
Mutation：A334V No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 10；277 K；VAPOR DIFFUSION, HANGING DROP, 277K, PH 10.0, 1M AMMONIUM SULPHATE 0.1M NA- PHOSPHATE
Resolution 2.00 Å
1QA3 TAILSPIKE PROTEIN, MUTANT A334I Deposited 1999-04-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 114–667（554 aa） Fragment：RECEPTOR BINDING C-TERMINAL FRAGMENT
Mutation：A334I No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 10；277 K；VAPOR DIFFUSION, HANGING DROP, 277K, PH 10.0, 1M AMMONIUM SULPHATE 0.1M NA- PHOSPHATE
Resolution 2.00 Å
1QA3 TAILSPIKE PROTEIN, MUTANT A334I Deposited 1999-04-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 114–667（554 aa） Fragment：RECEPTOR BINDING C-TERMINAL FRAGMENT
Mutation：A334I No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 10；277 K；VAPOR DIFFUSION, HANGING DROP, 277K, PH 10.0, 1M AMMONIUM SULPHATE 0.1M NA- PHOSPHATE
Resolution 2.00 Å
1QQ1 TAILSPIKE PROTEIN, MUTANT E359G Deposited 1999-06-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 109–667（559 aa） Fragment：C-TERMINAL FRAGMENT
Mutation：E359G No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 10；293 K；1M AMMONIUM SULPHATE, 0.1M SODIUM PHOSPHATE, pH 10.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.80 Å
1QRB PLASTICITY AND STERIC STRAIN IN A PARALLEL BETA-HELIX: RATIONAL MUTATIONS IN P22 TAILSPIKE PROTEIN Deposited 1999-06-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 109–667（559 aa） Fragment：C-TERMINAL FRAGMENT
Mutation：T326F No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 10；293 K；1M AMMONIUM SULPHATE 0.1 M SODIUM PHOSPHATE, pH 10.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.00 Å
1QRC TAILSPIKE PROTEIN, MUTANT W391A Deposited 1999-06-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 109–667（559 aa） Fragment：C-TERMINAL FRAGMENT
Mutation：W391A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 10；293 K；1M AMMONIUM SULPHATE, 0.1M NA-PHOSPHATE, pH 10.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.50 Å
1TSP CRYSTAL STRUCTURE OF P22 TAILSPIKE PROTEIN: INTERDIGITATED SUBUNITS IN A THERMOSTABLE TRIMER Deposited 1994-06-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 109–667（559 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
1TYU STRUCTURE OF TAILSPIKE-PROTEIN Deposited 1996-07-26 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 114–667（554 aa） Fragment：RESIDUES 109-666 LACKING THE N-TERMINAL, HEAD-BINDING DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；COMPLEX FORMED BY SOAKING WITH 2MM OCTASACCHARIDE FROM SALMONELLA ENTERITIDIS AT PH 7.5
Resolution 1.80 Å
1TYV STRUCTURE OF TAILSPIKE-PROTEIN Deposited 1996-07-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 114–667（554 aa） Fragment：RESIDUES 109-666 LACKING THE N-TERMINAL, HEAD-BINDING DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；pH 7.5
Resolution 1.80 Å
1TYW STRUCTURE OF TAILSPIKE-PROTEIN Deposited 1996-07-26 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 114–667（554 aa） Fragment：RESIDUES 109-666 LACKING THE N-TERMINAL, HEAD-BINDING DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；COMPLEX FORMED BY SOAKING WITH DECASACCHARIDE FROM S. TYPHI 253TY O-ANTIGEN AT PH 7.5
Resolution 1.80 Å
1TYX TITLE OF TAILSPIKE-PROTEIN Deposited 1996-07-26 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 114–667（554 aa） Fragment：RESIDUES 109-666 LACKING THE N-TERMINAL, HEAD-BINDING DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；COMPLEX FORMED BY SOAKING WITH 2MM OCTASACCHARIDE FROM SALMONELLA TYPHIMURIUM O-ANTIGEN AT PH 7.5.
Resolution 1.80 Å
2VFM Low Temperature Structure of P22 Tailspike Protein Fragment (109-666) Deposited 2007-11-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 110–667（558 aa） Fragment：RESIDUES 110-667
Not recorded GOL GLYCEROL × 33 SO4 SULFATE ION × 6 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 10；DROP: 2 MICROLITER 1.5 M AMMONIUM SULFATE, 0.1 M SODIUM PHOSPHATE, PH 10.0, PLUS 3.3 MICROLITER 10 MG/ML PROTEIN SOLUTION IN 10 MM HEPES, PH 7.0; RESERVOIR: 750 MICOLITER 1.0 M AMMONIUM SULFATE, 0.1 M SODIUM PHOSPHATE, PH 10.0
Resolution 1.50 Å R-free 0.164
2VFN Low Temperature Structure of P22 Tailspike Protein Fragment (109-666), Mutant V125A Deposited 2007-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 110–667（558 aa） Fragment：RESIDUES 110-667
Mutation：YES GOL GLYCEROL × 30 SO4 SULFATE ION × 3 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 10；DROP: 2 MICROLITER 1.5 M AMMONIUM SULFATE, 0.1 M SODIUM PHOSPHATE, PH 10.0, PLUS 3.3 MICROLITER, 10 MG/ML PROTEIN SOLUTION IN 10 MM HEPES, PH 7.0; RESERVOIR: 750 MICOLITER 1.0 M AMMONIUM SULFATE, 0.1 M SODIUM PHOSPHATE, PH 10.0
Resolution 1.50 Å R-free 0.155
2VFO Low Temperature Structure of P22 Tailspike Protein Fragment (109-666), Mutant V125L Deposited 2007-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 110–667（558 aa） Fragment：RESIDUES 110-667
Mutation：YES GOL GLYCEROL × 21 SO4 SULFATE ION × 3 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 10；DROP: 2 MICROLITER 1.5 M AMMONIUM SULFATE, 0.1 M SODIUM PHOSPHATE, PH 10.0, PLUS 3.3 MICROLITER 10 MG/ML PROTEIN SOLUTION IN 10 MM HEPES, PH 7.0; RESERVOIR: 750 MICOLITER 1.0 M AMMONIUM SULFATE, 0.1 M SODIUM PHOSPHATE, PH 10.0
Resolution 1.50 Å R-free 0.144
2VFP Low Temperature Structure of P22 Tailspike Protein Fragment (109-666), Mutant V349L Deposited 2007-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 110–667（558 aa） Fragment：RESIDUES 110-667
Mutation：YES GOL GLYCEROL × 18 SO4 SULFATE ION × 3 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 10；DROP: 2 MICROLITER 1.5 M AMMONIUM SULFATE, 0.1 M SODIUM PHOSPHATE, PH 10.0, PLUS 3.3 MICROLITER 10 MG/ML PROTEIN SOLUTION IN 10 MM HEPES, PH 7.0; RESERVOIR: 750 MICOLITER 1.0 M AMMONIUM SULFATE, 0.1 M SODIUM PHOSPHATE, PH 10.0
Resolution 1.55 Å R-free 0.152
2VFQ Low Temperature Structure of P22 Tailspike Protein Fragment (109-666), Mutant V450A Deposited 2007-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 110–667（558 aa） Fragment：RESIDUES 110-667 LACKING THE N-TERMINAL HEAD-BINDING DOMAIN
Mutation：YES GOL GLYCEROL × 12 SO4 SULFATE ION × 6 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 10；DROP: 2 MICROLITER 1.5 M AMMONIUM SULFATE, 0.1 M SODIUM PHOSPHATE, PH 10.0, PLUS 3.3 MICROLITER 10 MG/ML PROTEIN SOLUTION IN 10 MM HEPES, PH 7.0; RESERVOIR: 750 MICOLITER 1.0 M AMMONIUM SULFATE, 0.1 M SODIUM PHOSPHATE, PH 10.0
Resolution 1.55 Å R-free 0.145
2VKY Headbinding Domain of Phage P22 Tailspike C-Terminally Fused to Isoleucine Zipper pIIGCN4 (Chimera I) Deposited 2008-01-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain B 2–124（123 aa） Fragment：HEAD-BINDING DOMAIN, RESIDUES 2-124
Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；VAPOR DIFFUSION, HANGING DROP. PROTEIN: CONC. 8.2 MG/ML,BUFFER 50MM HEPES, PH6.5; RESERVOIR: 20% ISOPROPANOL, 0.1M NA-ACETATE, PH4.6, 0.2M CACL2; DROPLET 2 MICROL: 2 MICROL.CRYO:30% GLYCEROL.
Resolution 2.05 Å R-free 0.187
2VNL MUTANT Y108Wdel OF THE HEADBINDING DOMAIN OF PHAGE P22 TAILSPIKE C- TERMINally fused to ISOLEUCINE ZIPPER pIIGCN4 (chimera II) Deposited 2008-02-05 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 2–122（121 aa） Fragment：HEAD-BINDING DOMAIN, RESIDUES 2-122
Mutation：YES GOL GLYCEROL × 9 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；VAPOR DIFFUSION, HANGING DROP. PROTEIN: CONC. 9.3 MG/ML,BUFFER 50MM HEPES, PH6.5; RESERVOIR:20% ISOPROPANOL, 0.1M NA-ACETATE, PH4.6, 0.2M CACL2; DROPLET 2 MICROL:2 MICROL.CRYO:30% GLYCEROL.
Resolution 1.80 Å R-free 0.229
2XC1 Full-length Tailspike Protein Mutant Y108W of Bacteriophage P22 Deposited 2010-04-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 2–667（666 aa） Fragment：RESIDUES 2-667
Chain B 2–667（666 aa） Fragment：RESIDUES 2-667
Chain C 2–667（666 aa） Fragment：RESIDUES 2-667
Mutation：YES Mutation：YES Mutation：YES GOL GLYCEROL × 13 CA CALCIUM ION × 1 PE4 2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL × 4 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；292 K；PROTEIN- 15MG/ML IN 10MM HEPES PH7;RESERVOIR:750 ML 0.2M AMMONIUM ACETATE,0.1M TRI-SODIUM CITRATE DIHYDRATE PH 5.6, 30% W/V POLYETHYLENE GLYCOL 4000; HANGING DROPS:1.5MICROL RESERVOIR- 1.5MICROL PROTEIN SOLUTION; TEMPERATURE: 19 DEGR.; CRYO: 3% GLYCEROL
Resolution 1.65 Å R-free 0.210
5GAI Probabilistic Structural Models of Mature P22 Bacteriophage Portal, Hub, and Tailspike proteins Deposited 2015-12-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric
Chain 0 6–667（662 aa）
Chain Y 6–667（662 aa）
Chain Z 6–667（662 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 2 seconds before plunging.
Resolution 10.50 Å
8EAN Cryo-EM structure of in-situ tailspike in bacteriophage P22 Deposited 2022-08-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain 0 6–667（662 aa）
Chain Y 6–667（662 aa）
Chain Z 6–667（662 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8EB7 Cryo-EM structure of the in-situ gp4-gp10-gp9N from bacteriophage P22 Deposited 2022-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain 0 6–116（111 aa）
Chain A 6–116（111 aa）
Chain B 6–116（111 aa）
Chain C 6–116（111 aa）
Chain D 6–116（111 aa）
Chain F 6–116（111 aa）
Chain X 6–116（111 aa）
Chain Y 6–116（111 aa）
Chain Z 6–116（111 aa）
Chain a 6–116（111 aa）
Chain b 6–116（111 aa）
Chain c 6–116（111 aa）
Chain d 6–116（111 aa）
Chain e 6–116（111 aa）
Chain f 6–116（111 aa）
Chain g 6–116（111 aa）
Chain h 6–116（111 aa）
Chain i 6–116（111 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8TVR In situ cryo-EM structure of bacteriophage P22 tail hub protein: tailspike protein complex at 2.8A resolution Deposited 2023-08-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain A 1–667（667 aa）
Chain B 1–667（667 aa）
Chain C 1–667（667 aa）
Chain D 1–667（667 aa）
Chain E 1–667（667 aa）
Chain F 1–667（667 aa）
Chain H 1–667（667 aa）
Chain I 1–667（667 aa）
Chain J 1–667（667 aa）
Chain L 1–667（667 aa）
Chain M 1–667（667 aa）
Chain N 1–667（667 aa）
Chain P 1–667（667 aa）
Chain Q 1–667（667 aa）
Chain R 1–667（667 aa）
Chain V 1–667（667 aa）
Chain W 1–667（667 aa）
Chain X 1–667（667 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
8U10 In situ cryo-EM structure of bacteriophage P22 gp1:gp4:gp5:gp10:gp9 N-term complex in conformation 1 at 3.2A resolution Deposited 2023-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 58 PDB declaration: 58-meric
Chain 10 1–667（667 aa）
Chain 11 1–667（667 aa）
Chain 12 1–667（667 aa）
Chain 13 1–667（667 aa）
Chain 14 1–667（667 aa）
Chain 15 1–667（667 aa）
Chain 16 1–667（667 aa）
Chain 17 1–667（667 aa）
Chain 18 1–667（667 aa）
Chain 19 1–667（667 aa）
Chain 20 1–667（667 aa）
Chain 21 1–667（667 aa）
Chain 22 1–667（667 aa）
Chain 23 1–667（667 aa）
Chain 24 1–667（667 aa）
Chain 7 1–667（667 aa）
Chain 8 1–667（667 aa）
Chain 9 1–667（667 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8U11 In situ cryo-EM structure of bacteriophage P22 gp1:gp5:gp4: gp10: gp9 N-term complex in conformation 2 at 3.1A resolution Deposited 2023-08-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 58 PDB declaration: 58-meric
Chain 10 1–667（667 aa）
Chain 11 1–667（667 aa）
Chain 12 1–667（667 aa）
Chain 13 1–667（667 aa）
Chain 14 1–667（667 aa）
Chain 15 1–667（667 aa）
Chain 16 1–667（667 aa）
Chain 17 1–667（667 aa）
Chain 18 1–667（667 aa）
Chain 19 1–667（667 aa）
Chain 20 1–667（667 aa）
Chain 21 1–667（667 aa）
Chain 22 1–667（667 aa）
Chain 23 1–667（667 aa）
Chain 24 1–667（667 aa）
Chain 7 1–667（667 aa）
Chain 8 1–667（667 aa）
Chain 9 1–667（667 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8U1O In situ cryo-EM structure of bacteriophage P22 tailspike protein complex at 3.4A resolution Deposited 2023-09-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain o 1–667（667 aa）
Chain p 1–667（667 aa）
Chain q 1–667（667 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å

28 other PDB entries and 33 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name TSPE_BPP22
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–559； UniProt 109–667

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 3th0

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 3th0
Download Download
▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id3th0
Deposition date deposition_date2011-08-18
Structure title titleP22 Tailspike complexed with S.Paratyphi O antigen octasaccharide
Keywords keywords;VIRAL ADHESION PROTEIN, RECEPTOR, ENDOGLYCOSIDASE, CARBOHYDRATE, CELL RECEPTOR, RECOGNITION, BINDING PROTEIN LIPOPOLYSACCHARIDE, beta helix, host recognition, Bacteriophage P22 baseplate, HYDROLASE, VIRAL PROTEIN ;; VIRAL PROTEIN
Experimental Method methodX-RAY DIFFRACTION
▼

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier32.02 Å
Radius of gyration Rg (electron density) rg_electron32.99 Å
Forward intensity I(0) i060201600.00
Molecular weight molecular_weight60850.0 kDa
Excluded volume excluded_volume75993 ų
Envelope volume envelope_volume99427 ų
Hydration-shell volume shell_volume28730 ų
Envelope diameter envelope_diameter135.1 Å
Shell Rg shell_rg34.23 Å
Envelope Rg envelope_rg34.49 Å
Shape Rg shape_rg32.98 Å
Total Rg total_rg33.12 Å
Total atoms total_atoms4283
Residues n_residues552
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
▼

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax128.4 Å
Rg (real space) rg_real32.80 Å
Rg uncertainty (real space) rg_real_error1.67 Å
I(0) (real space) i0_real6.0200e+07
I(0) uncertainty (real space) i0_real_error1.1560e+06
Rg (reciprocal space) rg_reciprocal32.47 Å
I(0) (reciprocal space) i0_reciprocal60180000.0000
Solution quality estimate total_estimate0.6792
Solution quality rating solution_quality REASONABLE a REASONABLE solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary28.8 Å
Skewness Skewness skewness0.815
Kurtosis Kurtosis kurtosis0.174
Angular range angular_range— – 0.2450 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha9675000.0000
Real-space data points n_real_points50
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.230; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.157; Smooth: 0.978
▶

5. Crystallography and Experiment 5. Crystallography & Experiment

▶

6. Entities and Polymers Entities & Polymers (4)

▼

7. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

Domain ID domain_idd3th0a_
Class classb — All beta proteins
Fold Fold foldb.80 — Single-stranded right-handed beta-helix
Superfamily Superfamily superfamilyb.80.1 — Pectin lyase-like
Family Family familyb.80.1.6 — P22 tailspike protein

CATH v4.4 (1 domains)

Domain ID domain_id3th0A00
Class class2 — Mainly Beta
Architecture architecture160 — 3 Solenoid
Topology topology20 — Pectate Lyase C-like
Homologous superfamily homologous superfamily20 —
▶

8. Citations (3)

▶

9. Files and Curves (10)