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4daj

Structure of the M3 Muscarinic Acetylcholine Receptor

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Muscarinic acetylcholine receptor M3, Lysozyme

Enterobacteria phage T4

UniProt P00720

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Insufficient information Monomer Protein 1 (1R,2R,4S,5S,7S)-7-{[hydroxy(dithiophen-2-yl)acetyl]oxy}-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.0~2,4~]nonane × 1 PHOSPHATE ION × 4 Consistent with protein count
2 Insufficient information Monomer Protein 1 (1R,2R,4S,5S,7S)-7-{[hydroxy(dithiophen-2-yl)acetyl]oxy}-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.0~2,4~]nonane × 1 PHOSPHATE ION × 1 Consistent with protein count
3 Insufficient information Monomer Protein 1 (1R,2R,4S,5S,7S)-7-{[hydroxy(dithiophen-2-yl)acetyl]oxy}-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.0~2,4~]nonane × 1 PHOSPHATE ION × 2 Consistent with protein count
4 Insufficient information Monomer Protein 1 (1R,2R,4S,5S,7S)-7-{[hydroxy(dithiophen-2-yl)acetyl]oxy}-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.0~2,4~]nonane × 1 PHOSPHATE ION × 3 Consistent with protein count
5 Insufficient information Homooligomer Protein 2 (1R,2R,4S,5S,7S)-7-{[hydroxy(dithiophen-2-yl)acetyl]oxy}-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.0~2,4~]nonane × 2 PHOSPHATE ION × 5 Consistent with protein count
6 Insufficient information Homooligomer Protein 2 (1R,2R,4S,5S,7S)-7-{[hydroxy(dithiophen-2-yl)acetyl]oxy}-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.0~2,4~]nonane × 2 PHOSPHATE ION × 5 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
102l 103l 104l 104l 107l 108l 109l 110l 111l 112l 113l 114l

656 assemblies; showing the first 12

Homodimer
139l 1lwg 2huk 2hul 2hum 3fad 3hwl 3sb6 3sb7 3sb8 3sb9 4ekp

15 assemblies; showing the first 12

Homotetramer
3sbb

1 assemblies

Other homooligomers
1t8g 3sb5 3sb5 3sb5 3sb5 3sb7 3sba

7 assemblies

Heteromeric or nucleic-acid complex
148l 29aj 2qb0 2qb0 2rh1 3d4s 3eml 3ny8 3ny9 3nya 3odu 3oe0

168 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name LYS_BPT4
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 205–365； UniProt 1–161 Author chain B； PDBConstruct 205–365； UniProt 1–161 Author chain C； PDBConstruct 205–365； UniProt 1–161 Author chain D； PDBConstruct 205–365； UniProt 1–161

Muscarinic acetylcholine receptor M3, Lysozyme

Enterobacteria phage T4

UniProt P08483

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Insufficient information Monomer Protein 1 (1R,2R,4S,5S,7S)-7-{[hydroxy(dithiophen-2-yl)acetyl]oxy}-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.0~2,4~]nonane × 1 PHOSPHATE ION × 4 Consistent with protein count
2 Insufficient information Monomer Protein 1 (1R,2R,4S,5S,7S)-7-{[hydroxy(dithiophen-2-yl)acetyl]oxy}-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.0~2,4~]nonane × 1 PHOSPHATE ION × 1 Consistent with protein count
3 Insufficient information Monomer Protein 1 (1R,2R,4S,5S,7S)-7-{[hydroxy(dithiophen-2-yl)acetyl]oxy}-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.0~2,4~]nonane × 1 PHOSPHATE ION × 2 Consistent with protein count
4 Insufficient information Monomer Protein 1 (1R,2R,4S,5S,7S)-7-{[hydroxy(dithiophen-2-yl)acetyl]oxy}-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.0~2,4~]nonane × 1 PHOSPHATE ION × 3 Consistent with protein count
5 Insufficient information Homooligomer Protein 2 (1R,2R,4S,5S,7S)-7-{[hydroxy(dithiophen-2-yl)acetyl]oxy}-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.0~2,4~]nonane × 2 PHOSPHATE ION × 5 Consistent with protein count
6 Insufficient information Homooligomer Protein 2 (1R,2R,4S,5S,7S)-7-{[hydroxy(dithiophen-2-yl)acetyl]oxy}-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.0~2,4~]nonane × 2 PHOSPHATE ION × 5 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
4daj 4daj 4daj 4daj 4daj 4daj 4u14 4u14 4u15 4u15 4u15 4u16

15 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name ACM3_RAT
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 2–204； UniProt 57–259 Author chain A； PDBConstruct 366–473； UniProt 482–589 Author chain B； PDBConstruct 2–204； UniProt 57–259 Author chain B； PDBConstruct 366–473； UniProt 482–589 Author chain C； PDBConstruct 2–204； UniProt 57–259 Author chain C； PDBConstruct 366–473； UniProt 482–589 Author chain D； PDBConstruct 2–204； UniProt 57–259 Author chain D； PDBConstruct 366–473； UniProt 482–589

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id4daj
Deposition date deposition_date2012-01-12
Structure title titleStructure of the M3 Muscarinic Acetylcholine Receptor
Keywords keywordsG protein-coupled receptor, muscarinic receptor, Acetylcholine receptor, SIGNALING PROTEIN, Hydrolase; SIGNALING PROTEIN, Hydrolase
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

4daj__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

4daj__assembly_1__model_1 | I(q)

10-2 10-1 105 106 107 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

4daj__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）28.78 Å
Rg (electron density)28.03 Å
Total Rg28.67 Å
Atom count3451
Residues429
Excluded volume62329 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 4daj__assembly_1__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 4daj__assembly_2__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
3 1 4daj__assembly_3__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
4 1 4daj__assembly_4__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
5 1 4daj__assembly_5__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
6 1 4daj__assembly_6__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (3)

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6. Fold Classification (SCOP + CATH) 8 domains

CATH v4.4 (8 domains)

Domain ID domain_id4dajA01
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1070 — Rhopdopsin 7-helix transmembrane proteins
Homologous superfamily homologous superfamily10 — Rhodopsin 7-helix transmembrane proteins
Domain ID domain_id4dajA02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology530 — Lysozyme
Homologous superfamily homologous superfamily40 —
Domain ID domain_id4dajB01
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1070 — Rhopdopsin 7-helix transmembrane proteins
Homologous superfamily homologous superfamily10 — Rhodopsin 7-helix transmembrane proteins
Domain ID domain_id4dajB02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology530 — Lysozyme
Homologous superfamily homologous superfamily40 —
Domain ID domain_id4dajC01
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1070 — Rhopdopsin 7-helix transmembrane proteins
Homologous superfamily homologous superfamily10 — Rhodopsin 7-helix transmembrane proteins
Domain ID domain_id4dajC02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology530 — Lysozyme
Homologous superfamily homologous superfamily40 —
Domain ID domain_id4dajD01
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1070 — Rhopdopsin 7-helix transmembrane proteins
Homologous superfamily homologous superfamily10 — Rhodopsin 7-helix transmembrane proteins
Domain ID domain_id4dajD02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology530 — Lysozyme
Homologous superfamily homologous superfamily40 —
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7. Citations (1)