Thermonuclease
Staphylococcus aureus
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain A； UniProt 83–231 | Fragment：Nuclease A (UNP residues 83-231) Mutation：L25A,G50F,V51N,V66A,I92A,P117G,H124L,S128A,del(44-49) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, vapor diffusion, hanging drop, temperature 277K, VAPOR DIFFUSION, HANGING DROP | Resolution 1.75 Å R-free 0.219 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 4KD3 | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1A2T STAPHYLOCOCCAL NUCLEASE, B-MERCAPTOETHANOL DISULFIDE TO V23C VARIANT Deposited 1998-01-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23C, B-MERCAPTOETHANOL DISULFIDE Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.15；277 K；10 MM POTASSIUM PHOSPHATE, PH 8.15 PROTEIN CONCENTRATION = 2 MGS/ML MPD =21% T=4 DEGREES C, temperature 277K
|
Resolution 1.96 Å |
| 1A2U STAPHYLOCOCCAL NUCLEASE, V23C VARIANT, COMPLEX WITH 1-N-BUTANE THIOL AND 3',5'-THYMIDINE DIPHOSPHATE Deposited 1998-01-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23C, S-THIOBUTYL DISULFIDE Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.15；277 K；10 MM POTASSIUM PHOSPHATE, PH 8.15 PROTEIN CONCENTRATION = 2 MGS/ML MPD =21% T=4 DEGREES C, temperature 277K
|
Resolution 2.00 Å |
| 1A3T STAPHYLOCOCCAL NUCLEASE, V23C VARIANT, COMPLEX WITH 2-FLUOROETHANE THIOL AND 3',5'-THYMIDINE DIPHOSPHATE Deposited 1998-01-24 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23C, S-THIOETHYLFLUORO DISULFIDE Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.15；277 K；10 MM POTASSIUM PHOSPHATE, PH 8.15 PROTEIN CONCENTRATION = 2 MGS/ML MPD =21% T=4 DEGREES C, temperature 277K
|
Resolution 2.10 Å |
| 1A3U STAPHYLOCOCCAL NUCLEASE, CYCLOHEXANE THIOL DISULFIDE TO V23C VARIANT Deposited 1998-01-24 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23C, CYCLOHEXANE THIOL DISULFIDE Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.15；277 K；10 MM POTASSIUM PHOSPHATE, PH 8.15 PROTEIN CONCENTRATION = 2 MGS/ML MPD =21% T=4 DEGREES C, temperature 277K
|
Resolution 2.05 Å |
| 1A3V STAPHYLOCOCCAL NUCLEASE, CYCLOPENTANE THIOL DISULFIDE TO V23C VARIANT Deposited 1998-01-24 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23C, CYCLOPENTANE THIOL DISULFIDE Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.15；277 K；10 MM POTASSIUM PHOSPHATE, PH 8.15 PROTEIN CONCENTRATION = 2 MGS/ML MPD =21% T=4 DEGREES C, temperature 277K
|
Resolution 2.20 Å |
| 1AEX STAPHYLOCOCCAL NUCLEASE, METHANE THIOL DISULFIDE TO V23C VARIANT Deposited 1997-03-01 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23C Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.15；10 MM POTASSIUM PHOSPHATE, PH = 8.15, PROTEIN CONC. = 2 MGS/ML, MPD = 21%, T = 4 C
|
Resolution 2.10 Å |
| 1ENA CRYSTAL STRUCTURES OF THE BINARY CA2+ AND PDTP COMPLEXES AND THE TERNARY COMPLEX OF THE ASP 21->GLU MUTANT OF STAPHYLOCOCCAL NUCLEASE. IMPLICATIONS FOR CATALYSIS AND LIGAND BINDING Deposited 1994-02-14 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
89–223（135 aa）
|
Not recorded | CA CALCIUM ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.15 Å |
| 1ENC CRYSTAL STRUCTURES OF THE BINARY CA2+ AND PDTP COMPLEXES AND THE TERNARY COMPLEX OF THE ASP 21->GLU MUTANT OF STAPHYLOCOCCAL NUCLEASE. IMPLICATIONS FOR CATALYSIS AND LIGAND BINDING Deposited 1994-02-14 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Not recorded | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.95 Å |
| 1EQV SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE Deposited 2000-04-06 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
88–223（136 aa）
|
Mutation：Y113A, V114A, Y115A, K116A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
SMALL TUBES；pH 8.15；293 K；10.5 mM potassium phosphate, MPD, pH 8.15, SMALL TUBES, temperature 293K
|
Resolution 1.94 Å |
| 1EY0 STRUCTURE OF WILD-TYPE S. NUCLEASE AT 1.6 A RESOLUTION Deposited 2000-05-05 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；MPD, Sodium Phosphate Buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.60 Å R-free 0.255 |
| 1EY4 STRUCTURE OF S. NUCLEASE STABILIZING MUTANT S59A Deposited 2000-05-05 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：S59A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；MPD,
Sodium phosphate buffer
, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.60 Å R-free 0.256 |
| 1EY5 STRUCTURE OF S. NUCLEASE STABILIZING MUTANT T33V Deposited 2000-05-05 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：T33V | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；MPD,
Sodium phosphate buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.70 Å R-free 0.266 |
| 1EY6 STRUCTURE OF S. NUCLEASE STABILIZING MUTANT T41I Deposited 2000-05-05 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：T41I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；MPD,
Sodium phosphate buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.75 Å R-free 0.263 |
| 1EY7 STRUCTURE OF S. NUCLEASE STABILIZING MUTANT S128A Deposited 2000-05-05 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：S128A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；MPD,
Sodium phosphate buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.88 Å R-free 0.266 |
| 1EY8 STRUCTURE OF S. NUCLEASE STABILIZING TRIPLE MUTANT P117G/H124L/S128A Deposited 2000-05-05 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：P117G,H124L,S128A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；MPD,
Sodium phosphate buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.75 Å R-free 0.240 |
| 1EY9 STRUCTURE OF S. NUCLEASE STABILIZING QUADRUPLE MUTANT T41I/P117G/H124L/S128A Deposited 2000-05-05 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：T41I,P117G,H124L,S128A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；MPD,
Sodium phosphate buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.72 Å R-free 0.270 |
| 1EYA STRUCTURE OF S. NUCLEASE STABILIZING QUINTUPLE MUTANT T33V/T41I/P117G/H124L/S128A Deposited 2000-05-05 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：T33V,T41I,P117G,H124L,S128A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；MPD,
Sodium phosphate buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.288 |
| 1EYC STRUCTURE OF S. NUCLEASE STABILIZING QUINTUPLE MUTANT T41I/S59A/P117G/H124L/S128A Deposited 2000-05-05 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：T41I/S59A/P117G/H124L/S128A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；MPD,
Sodium phosphate buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.85 Å R-free 0.250 |
| 1EYD STRUCTURE OF WILD-TYPE S. NUCLEASE AT 1.7 A RESOLUTION Deposited 2000-05-05 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；MPD,
Sodium phosphate buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.70 Å R-free 0.277 |
| 1EZ6 STRUCTURE OF S. NUCLEASE STABILIZING SEXTUPLE MUTANT T33V/T41I/S59A/P117G/H124L/S128A Deposited 2000-05-10 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：T33V/T41I/S59A/P117G/H124L/S128A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；MPD,
Sodium Phosphate Buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.279 |
| 1EZ8 STRUCTURE OF S. NUCLEASE STABILIZING MUTANT T33V Deposited 2000-05-10 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：T33V | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；MPD,
Sodium phosphate buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.85 Å R-free 0.265 |
| 1F2M SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE Deposited 2000-05-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V114A,K116A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.15；pH 8.15
|
Resolution 2.00 Å |
| 1F2Y SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE Deposited 2000-05-31 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：Y113A, K116A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
SMALL TUBES；pH 8.15；298 K；10.5 MM POTASSIUM PHOSPHATE, MPD, pH 8.15, SMALL TUBES, temperature 298K
|
Resolution 2.00 Å |
| 1F2Z SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE Deposited 2000-05-31 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：YES | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
SMALL TUBES；pH 8.15；298 K；10.5 MM POTASSIUM PHOSPHATE, MPD, pH 8.15, SMALL TUBES, temperature 298K
|
Resolution 1.80 Å |
| 1IHZ Structure of S. nuclease mutant quintuple mutant V23L/V66L/I72L/I92L/V99L Deposited 2001-04-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23L, V66L, I72L, I92L, V99L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；MPD, SODIUM PHOSPHATE BUFFER, pH 8.00, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.65 Å R-free 0.261 |
| 1II3 Structure of S. nuclease quintuple mutant V23I/V66L/I72L/I92L/V99L Deposited 2001-04-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23I,V66L,I72L,I92L,V99L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；MPD, SODIUM PHOSPHATE BUFFER, pH 8.00, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.72 Å R-free 0.270 |
| 1JOK Averaged structure for Staphylococcal nuclease-H124L in ternary complex with Ca2+ and thymidine-3',5'-bisphosphate Deposited 2001-07-30 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：nuclease A
|
Mutation：H124L | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
SOLUTION NMR
NMR measurement conditions
pH 5.5；318 K；Ionic strength (raw mmCIF value) 300 nM KCl；Pressure ambient
NMR measurement conditions
pH 5.1；318 K；Ionic strength (raw mmCIF value) 300 nM KCl；Pressure ambient
NMR sample composition
3.5-5 mM Nuclease-H124L U-50% 2H; 300 mM KCl; D2O | D2O
NMR sample composition
3.5-5 mM Nuclease-H124L U-95% 15N; 300 mM KCl; H2O | H2O
NMR sample composition
3.5-5 mM Nuclease-H124L; 300 mM KCl; D2O | D2O
NMR sample composition
3.5-5 mM Nuclease-H124L U-50% 2H; 300 mM KCl; H2O | H2O
NMR sample composition
3.5-5 mM Nuclease-H124L U-98% 13C; 300 mM KCl; H2O | H2O
NMR sample composition
3.5-5 mM Nuclease-H124L U-98% 13C U-99% 15N; 300 mM KCl; H2O | H20
|
Resolution not provided |
| 1JOO Averaged structure for unligated Staphylococcal nuclease-H124L Deposited 2001-07-30 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：nuclease A
|
Mutation：H124L | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 5.5；318 K；Ionic strength (raw mmCIF value) 300 nM KCl；Pressure ambient
NMR measurement conditions
pH 5.1；318 K；Ionic strength (raw mmCIF value) 300 nM KCl；Pressure ambient
NMR sample composition
3.5-5 mM Nuclease-H124L U-50% 2H; 300 mM KCl; D2O | D2O
NMR sample composition
3.5-5 mM Nuclease-H124L U-95% 15N; 300 mM KCl; H2O | H2O
NMR sample composition
3.5-5 mM Nuclease-H124L; 300 mM KCl; D2O | D2O
NMR sample composition
3.5-5 mM Nuclease-H124L U-50% 2H; 300 mM KCl; H2O | H2O
NMR sample composition
3.5-5 mM Nuclease-H124L U-98% 13C; 300 mM KCl; H2O | H2O
NMR sample composition
3.5-5 mM Nuclease-H124L U-98% 13C U-99% 15N; 300 mM KCl; H2O | H2O
|
Resolution not provided |
| 1JOQ Ensemble structures for Staphylococcal nuclease-H124L in ternary complex with Ca2+ and thymidine-3',5'-bisphosphate Deposited 2001-07-30 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：nuclease A
|
Mutation：H124L | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
SOLUTION NMR
NMR measurement conditions
pH 5.5；318 K；Ionic strength (raw mmCIF value) 300 nM KCl；Pressure ambient
NMR measurement conditions
pH 5.1；318 K；Ionic strength (raw mmCIF value) 300 nM KCl；Pressure ambient
NMR sample composition
3.5-5 mM Nuclease-H124L U-50% 2H; 300 mM KCl; D2O | D2O
NMR sample composition
3.5-5 mM Nuclease-H124L U-95% 15N; 300 mM KCl; H2O | H2O
NMR sample composition
3.5-5 mM Nuclease-H124L; 300 mM KCl; D2O | D2O
NMR sample composition
3.5-5 mM Nuclease-H124L U-50% 2H; 300 mM KCl; H2O | H2O
NMR sample composition
3.5-5 mM Nuclease-H124L U-98% 13C; 300 mM KCl; H2O | H2O
NMR sample composition
3.5-5 mM Nuclease-H124L U-98% 13C U-99% 15N; 300 mM KCl; H2O | H2O
|
Resolution not provided |
| 1JOR Ensemble structures for unligated Staphylococcal nuclease-H124L Deposited 2001-07-30 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：nuclease A
|
Mutation：H124L | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 5.5；318 K；Ionic strength (raw mmCIF value) 300 nM KCl；Pressure ambient
NMR measurement conditions
pH 5.1；318 K；Ionic strength (raw mmCIF value) 300 nM KCl；Pressure ambient
NMR sample composition
3.5-5 mM Nuclease-H124L U-50% 2H; 300 mM KCl; D2O | D2O
NMR sample composition
3.5-5 mM Nuclease-H124L U-95% 15N; 300 mM KCl; H2O | H2O
NMR sample composition
3.5-5 mM Nuclease-H124L; 300 mM KCl; D2O | D2O
NMR sample composition
3.5-5 mM Nuclease-H124L U-50% 2H; 300 mM KCl; H2O | H2O
NMR sample composition
3.5-5 mM Nuclease-H124L U-98% 13C; 300 mM KCl; H2O | H2O
NMR sample composition
3.5-5 mM Nuclease-H124L U-98% 13C U-99% 15N; 300 mM KCl; H2O | H2O
|
Resolution not provided |
| 1KAA STRESS AND STRAIN IN STAPHYLOCOCCAL NUCLEASE Deposited 1992-12-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
88–223（136 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å |
| 1KAB STRESS AND STRAIN IN STAPHYLOCOCCAL NUCLEASE Deposited 1992-12-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
88–223（136 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.80 Å |
| 1KDA STABILIZATION OF A STRAINED PROTEIN LOOP CONFORMATION THROUGH PROTEIN ENGINEERING Deposited 1994-08-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å |
| 1KDB STABILIZATION OF A STRAINED PROTEIN LOOP CONFORMATION THROUGH PROTEIN ENGINEERING Deposited 1994-08-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å |
| 1KDC STABILIZATION OF A STRAINED PROTEIN LOOP CONFORMATION THROUGH PROTEIN ENGINEERING Deposited 1994-08-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1NSN THE CRYSTAL STRUCTURE OF ANTIBODY N10-STAPHYLOCOCCAL NUCLEASE COMPLEX AT 2.9 ANGSTROMS RESOLUTION Deposited 1995-06-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain S
83–231（149 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.80 Å |
| 1NUC STAPHYLOCOCCAL NUCLEASE, V23C VARIANT Deposited 1997-02-15 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23C | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.15；277 K；10 MM POTASSIUM PHOSPHATE, PH = 8.15, PROTEIN CONC. = 2 MGS/ML, MPD = 21%, T = 4 C, temperature 277K
|
Resolution 1.90 Å |
| 1RKN Solution structure of 1-110 fragment of Staphylococcal Nuclease with G88W mutation Deposited 2003-11-22 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–192（110 aa）
Fragment：residues 1-110
|
Mutation：G88W | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 4.9；305 K；Ionic strength (raw mmCIF value) 0.05；Pressure ambient
NMR sample composition
1-2mM G88W110-15N,13C labeled; 50mM Acetate buffer, DSS, NaN3; 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition
1-2mM G88W110-13C labeled; 50mM Acetate buffer, DSS, NaN3; 100% D2O | 100% D2O
NMR sample composition
1-2mM G88W110-15N labeled; 50mM Acetate buffer, DSS, NaN3; 90% H2O, 10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 1SNC THE CRYSTAL STRUCTURE OF THE TERNARY COMPLEX OF STAPHYLOCOCCAL NUCLEASE, CA2+, AND THE INHIBITOR PD*TP, REFINED AT 1.65 ANGSTROMS Deposited 1989-07-21 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Not recorded | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.65 Å |
| 1SND STAPHYLOCOCCAL NUCLEASE DIMER CONTAINING A DELETION OF RESIDUES 114-119 COMPLEXED WITH CALCIUM CHLORIDE AND THE COMPETITIVE INHIBITOR DEOXYTHYMIDINE-3',5'-DIPHOSPHATE Deposited 1996-08-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
83–231（149 aa）
Chain B
83–231（149 aa）
|
Mutation：DEL(114-119) Mutation：DEL(114-119) | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.84 Å |
| 1SND STAPHYLOCOCCAL NUCLEASE DIMER CONTAINING A DELETION OF RESIDUES 114-119 COMPLEXED WITH CALCIUM CHLORIDE AND THE COMPETITIVE INHIBITOR DEOXYTHYMIDINE-3',5'-DIPHOSPHATE Deposited 1996-08-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
83–231（149 aa）
Chain B
83–231（149 aa）
|
Mutation：DEL(114-119) Mutation：DEL(114-119) | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.84 Å |
| 1SNM ACTIVE SITE MUTANT GLU-43 (RIGHT ARROW) ASP IN STAPHYLOCOCCAL NUCLEASE DISPLAYS NONLOCAL STRUCTURAL CHANGES Deposited 1990-02-15 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：D43E | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.74 Å |
| 1SNO PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE Deposited 1996-07-06 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.15；10.5 MM POTASSIUM PHOSPHATE, 20% - 30% 2-METHYL-2,4-PENTANEDIOL, pH 8.15
|
Resolution 1.70 Å R-free 0.224 |
| 1SNP PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE Deposited 1996-07-06 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：P117G | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.15；10.5 MM POTASSIUM PHOSPHATE, 20% - 30% 2-METHYL-2,4-PENTANEDIOL, pH 8.15
|
Resolution 1.95 Å R-free 0.255 |
| 1SNQ PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE Deposited 1996-07-06 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：P47G, P117G | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.15；10.5 MM POTASSIUM PHOSPHATE, 20% - 30% 2-METHYL-2,4-PENTANEDIOL, pH 8.15
|
Resolution 1.70 Å R-free 0.274 |
| 1STA ACCOMMODATION OF INSERTION MUTATIONS ON THE SURFACE AND IN THE INTERIOR OF STAPHYLOCOCCAL NUCLEASE Deposited 1994-01-17 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–229（147 aa）
|
Not recorded | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.70 Å |
| 1STB ACCOMMODATION OF INSERTION MUTATIONS ON THE SURFACE AND IN THE INTERIOR OF STAPHYLOCOCCAL NUCLEASE Deposited 1994-01-17 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–230（148 aa）
|
Not recorded | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1STG TWO DISTINCTLY DIFFERENT METAL BINDING MODES ARE SEEN IN X-RAY CRYSTAL STRUCTURES OF STAPHYLOCOCCAL NUCLEASE-COBALT(II)-NUCLEOTIDE COMPLEXES Deposited 1994-10-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Not recorded | CO COBALT (II) ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
COMPOUND
CRYSTAL SOAKED FOR ONE WEEK AT 277 K IN STABILIZING
BUFFER COMPRISING 40% (WW) MPD IN 30 MM TRIS-CL PH 8.15,
50 UM COCL2, 100 UM POTASSIUM CITRATE AND 50 UM PDTP.
|
Resolution 1.70 Å |
| 1STH TWO DISTINCTLY DIFFERENT METAL BINDING MODES ARE SEEN IN X-RAY CRYSTAL STRUCTURES OF STAPHYLOCOCCAL NUCLEASE-COBALT(II)-NUCLEOTIDE COMPLEXES Deposited 1994-10-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Not recorded | CO COBALT (II) ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
CRYSTAL SOAKED FOR NINE MONTHS AT 277 K IN STABILIZING
BUFFER COMPRISING 40% (WW) MPD IN 30 MM TRIS-CL PH 8.15,
50 UM COCL2, 100 UM POTASSIUM CITRATE AND 50 UM PDTP.
SOME EVAPORATION OCCURRED DURING THIS PERIOD, SO MPD
CONCENTRATION AT TIME OF DATA COLLECTION, WHILE NOT
KNOWN PRECISELY, WAS SUBSTANTIALLY GREATER THAN 40%.
|
Resolution 1.85 Å |
| 1STN THE CRYSTAL STRUCTURE OF STAPHYLOCOCCAL NUCLEASE REFINED AT 1.7 ANGSTROMS RESOLUTION Deposited 1993-02-17 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.70 Å |
| 1STY THE ALPHA ANEURISM: A STRUCTURAL MOTIF REVEALED IN AN INSERTION MUTANT OF STAPHYLOCOCCAL NUCLEASE Deposited 1993-02-18 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–230（148 aa）
|
Not recorded | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.67 Å |
| 1SYB TRANSFER OF A BETA-TURN STRUCTURE TO A NEW PROTEIN CONTEXT Deposited 1994-01-07 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Not recorded | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.80 Å |
| 1SYC ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE Deposited 1994-01-07 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.80 Å |
| 1SYD ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE Deposited 1994-01-07 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Not recorded | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.70 Å |
| 1SYE ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE Deposited 1994-01-07 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.80 Å |
| 1SYF ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE Deposited 1994-01-07 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Not recorded | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.80 Å |
| 1SYG ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE Deposited 1994-01-07 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å |
| 1TQO Cryogenic Crystal Structure of Staphylococcal Nuclease Variant truncated Delta+PHS I92E Deposited 2004-06-17 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–226（144 aa）
|
Mutation：G50F, V51N, I92E, P117G, H124L, S128A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；300 K；MPD, potassium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 27K
|
Resolution 2.00 Å R-free 0.249 |
| 1TR5 Room temperature structure of Staphylococcal nuclease variant truncated Delta+PHS I92E Deposited 2004-06-20 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–226（144 aa）
|
Mutation：Deleteion of residues 44-49 and 145-149; point mutations G50F, V51N, I92E, P117G, H124L and S128A | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；MPD, potassium phosphate, glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.10 Å R-free 0.250 |
| 1TT2 Cryogenic crystal structure of Staphylococcal nuclease variant truncated Delta+PHS I92K Deposited 2004-06-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–226（144 aa）
|
Mutation：Deletion of residues 44-49 and 145-149; point mutations G50F, V51N, I92K, P117G, H124L, S128A | CA CALCIUM ION × 1 DMS DIMETHYL SULFOXIDE × 2 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；277 K；MPD, potassium phosphate, glycerol, DMSO, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.85 Å R-free 0.211 |
| 1U9R Crystal Structure of Staphylococcal Nuclease mutant V66E/P117G/H124L/S128A at Room Temperature Deposited 2004-08-10 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V66E/P117G/H124L/S128A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.4；277 K；MPD, potassium phosphate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.10 Å R-free 0.217 |
| 2ENB CRYSTAL STRUCTURES OF THE BINARY CA2+ AND PDTP COMPLEXES AND THE TERNARY COMPLEX OF THE ASP 21->GLU MUTANT OF STAPHYLOCOCCAL NUCLEASE. IMPLICATIONS FOR CATALYSIS AND LIGAND BINDING Deposited 1994-03-23 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
89–223（135 aa）
|
Not recorded | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.05 Å |
| 2EXZ Crystal structure of Staphylococcal nuclease mutant T22C Deposited 2005-11-09 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：T22C | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.265 |
| 2EY1 Crystal structure of Staphylococcal nuclease mutant T22V Deposited 2005-11-09 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：T22V | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.85 Å R-free 0.264 |
| 2EY2 Crystal structure of Staphylococcal nuclease mutant T41C Deposited 2005-11-09 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：T41C | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.70 Å R-free 0.266 |
| 2EY5 Crystal structure of Staphylococcal nuclease mutant T41S Deposited 2005-11-09 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：T41S | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.270 |
| 2EY6 Crystal structure of Staphylococcal nuclease mutant T41V Deposited 2005-11-09 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：T41V | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.65 Å R-free 0.247 |
| 2EYF Crystal structure of Staphylococcal nuclease mutant T44V Deposited 2005-11-09 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：T44V | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.80 Å R-free 0.266 |
| 2EYH Crystal structure of Staphylococcal nuclease mutant T62S Deposited 2005-11-09 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：T62S | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.261 |
| 2EYJ Crystal structure of Staphylococcal nuclease mutant T62V Deposited 2005-11-09 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：T62V | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.85 Å R-free 0.235 |
| 2EYL Crystal structure of Staphylococcal nuclease mutant T82S Deposited 2005-11-09 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：T82S | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.70 Å R-free 0.249 |
| 2EYM Crystal structure of Staphylococcal nuclease mutant T120C Deposited 2005-11-09 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：T120C | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.263 |
| 2EYO Crystal structure of Staphylococcal nuclease mutant T120S Deposited 2005-11-09 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：T120S | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.70 Å R-free 0.238 |
| 2EYP Crystal structure of Staphylococcal nuclease mutant T120V Deposited 2005-11-09 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：T120V | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.251 |
| 2F0D Crystal structure of Staphylococcal nuclease mutant I92V Deposited 2005-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：I92V | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.50 Å R-free 0.162 |
| 2F0E Crystal structure of Staphylococcal nuclease mutant V23L Deposited 2005-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.95 Å R-free 0.254 |
| 2F0F Crystal structure of Staphylococcal nuclease mutant L25I Deposited 2005-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：L25I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.15 Å R-free 0.288 |
| 2F0G Crystal structure of Staphylococcal nuclease mutant V66I Deposited 2005-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V66I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.252 |
| 2F0H Crystal structure of Staphylococcal nuclease mutant V66L Deposited 2005-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V66L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.15 Å R-free 0.282 |
| 2F0I Crystal structure of Staphylococcal nuclease mutant I72L Deposited 2005-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：I72L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.271 |
| 2F0J Crystal structure of Staphylococcal nuclease mutant I72V Deposited 2005-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：I72V | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.251 |
| 2F0K Crystal structure of Staphylococcal nuclease mutant V23I/L25I Deposited 2005-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23I/L25I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.262 |
| 2F0L Crystal structure of Staphylococcal nuclease mutant V23L/I72L Deposited 2005-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23L/I72L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.264 |
| 2F0M Crystal structure of Staphylococcal nuclease mutant V23L/I72V Deposited 2005-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23L/I72V | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.266 |
| 2F0N Crystal structure of Staphylococcal nuclease mutant L25I/I72L Deposited 2005-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：L25I/I72L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.70 Å R-free 0.280 |
| 2F0O Crystal structure of Staphylococcal nuclease mutant V66I/I72V Deposited 2005-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V66I/I72V | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.255 |
| 2F0P Crystal structure of Staphylococcal nuclease mutant V66I/V99I Deposited 2005-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V66I/V99I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.05 Å R-free 0.258 |
| 2F0Q Crystal structure of Staphylococcal nuclease mutant V66L/I92L Deposited 2005-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V66L/I92L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.95 Å R-free 0.270 |
| 2F0S Crystal structure of Staphylococcal nuclease mutant V66L/I92V Deposited 2005-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V66L/I92V | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.75 Å R-free 0.270 |
| 2F0T Crystal structure of Staphylococcal nuclease mutant V66L/V99I Deposited 2005-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V66L/V99I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.70 Å R-free 0.274 |
| 2F0U Crystal structure of Staphylococcal nuclease mutant V23I/L25I/I72V Deposited 2005-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23I/L25I/I72V | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.83 Å R-free 0.243 |
| 2F0V Crystal structure of Staphylococcal nuclease mutant V23L/V66L/I72L Deposited 2005-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23L/V66L/I72L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.254 |
| 2F0W Crystal structure of Staphylococcal nuclease mutant V23I/L25I/V66L/I72L Deposited 2005-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23I/L25I/V66L/I72L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.10 Å R-free 0.277 |
| 2F3V Solution structure of 1-110 fragment of staphylococcal nuclease with V66W mutation Deposited 2005-11-22 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–192（110 aa）
Fragment：Residues 1-110
|
Mutation：V66W | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 4.9；298 K；Ionic strength (raw mmCIF value) 50mM ACETATE BUFFER；Pressure ambient
NMR sample composition
2mM V66W110 fragment, 50MM ACETATE BUFFER, DSS, NAN3; 90% H2O, 10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2F3W solution structure of 1-110 fragment of staphylococcal nuclease in 2M TMAO Deposited 2005-11-22 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–192（110 aa）
Fragment：residues 1-110
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6；298 K；Ionic strength (raw mmCIF value) 2M TMAO, 0.2M HCl；Pressure ambient
NMR sample composition
1mM SNase110 fragment, 2M TMAO, 0.2M HCl, DSS, NAN3; 90% H2O, 10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2FXY Solution structure of 55-72 segment of staphylococcal nuclease Deposited 2006-02-07 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
137–154（18 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 5；298 K；Ionic strength (raw mmCIF value) 0；Pressure ambient
NMR sample composition
2.0mM peptides; DSS, NaN3; 40% trifluoroethanol-d4, 60% H2O | 40% trifluoroethanol-d4, 60% H2O
NMR sample composition
2.0mM peptides; DSS, NaN3; 40% trifluoroethanol-d4, 60% D2O | 40% trifluoroethanol-d4, 60% D2O
|
Resolution not provided |
| 2FXZ Solution structure of 97-109 segment of staphylococcal nuclease Deposited 2006-02-07 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
179–191（13 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 5；298 K；Ionic strength (raw mmCIF value) 0；Pressure ambient
NMR sample composition
2.0mM peptides; DSS, NaN3; 40% trifluoroethanol-d4, 60% H2O | 40% trifluoroethanol-d4, 60% H2O
NMR sample composition
2.0mM peptides; DSS, NaN3; 40% trifluoroethanol-d4, 60% D2O | 40% trifluoroethanol-d4, 60% D2O
|
Resolution not provided |
| 2KHS Solution structure of SNase121:SNase(111-143) complex Deposited 2009-04-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
83–203（121 aa）
Fragment：UNP residues 83-203
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 5；300 K；Ionic strength (raw mmCIF value) 0.15；Pressure ambient
NMR sample composition
1.0 mM [U-13C; U-15N] protein-1, 1.0 mM protein-2, 50 mM sodium acetate-3, 100 mM potassium chloride-4, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1.0 mM protein-1, 1.0 mM [U-13C; U-15N] protein-2, 50 mM sodium acetate-7, 100 mM potassium chloride-8, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1 mM [U-13C] protein-1, 1 mM [U-15N] protein-2, 50 mM sodium acetate-11, 100 mM potassium chloride-12, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1 mM [U-15N] protein-1, 1 mM [U-13C] protein-2, 50 mM sodium acetate-15, 100 mM potassium chloride-16, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2NUC STAPHLOCOCCAL NUCLEASE, ETHANE THIOL DISULFIDE TO V23C VARIANT Deposited 1997-12-06 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23C Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.15；10 MM POTASSIUM PHOSPHATE, PH 8.15 PROTEIN CONCENTRATION 2 MGS/ML, 21 PERCENT MPD, AT 4C.
|
Resolution 2.00 Å |
| 2OEO Cryogenic crystal structure of Staphylococcal Nuclease variant truncated Delta+PHS I92D Deposited 2006-12-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
80–223（144 aa）
|
Mutation：G50F, V51N, I92D, P117G, H124L, S128A | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20-35% MPD, 10% glycerol, 5% DMSO, 10.5 mM potassium phosphate in the presence of Ca2+ and pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K
|
Resolution 2.00 Å R-free 0.226 |
| 2OF1 Cryogenic crystal structure of the Staphylococcal Nuclease variant truncated Delta+PHS I92W Deposited 2007-01-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
80–223（144 aa）
|
Mutation：G50F, V51N, I92W, P117G, H124L, S128A | CA CALCIUM ION × 1 DMS DIMETHYL SULFOXIDE × 2 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；291 K；20-30% MPD, 10.5 mM potassium phosphate, 5% glycerol, 5% DMSO, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 1.92 Å R-free 0.244 |
| 2QDB Crystal structure of staphylococcal nuclease variant E75Q/D21N/T33V/T41I/S59A/P117G/S128A at 100 K Deposited 2007-06-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A
|
Mutation：E75Q,D21N,T33V,T41I,S59A,P117G,S128A | PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；38% MPD and 25 mM potassium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.276 |
| 2RBM Crystal structure of Staphylococcal nuclease variant Delta+PHS I72K at cryogenic temperature Deposited 2007-09-19 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：G50F,V51N,I72K,P117G,S128A,Del44-49 | CA CALCIUM ION × 1 PO4 PHOSPHATE ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；33% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.238 |
| 2RDF Crystal Structure of staphyloccocal nuclease VIAGAN/E75A variant at cryogenic temperature Deposited 2007-09-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A
|
Mutation：E75A,D21N,T33V,T41I,S59A,P117G,S128A | PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；37% MPD, 25 mM potassium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.01 Å R-free 0.244 |
| 2RKS Crystal structure of Staphylococcal nuclease variant PHS L38K at cryogenic temperature Deposited 2007-10-17 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：nuclease
|
Mutation：L38K,P117G,H124L,S128A | PO4 PHOSPHATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 10.5；277 K；45% MPD, 50 mM Potassium Phosphate, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.01 Å R-free 0.240 |
| 2SNM IN A STAPHYLOCOCCAL NUCLEASE MUTANT THE SIDE-CHAIN of A LYSINE REPLACING VALINE 66 IS FULLY BURIED IN THE HYDROPHOBIC CORE Deposited 1991-04-03 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V66K | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.97 Å |
| 2SNS STAPHYLOCOCCAL NUCLEASE. PROPOSED MECHANISM OF ACTION BASED ON STRUCTURE OF ENZYME-THYMIDINE 3(PRIME),5(PRIME)-BIPHOSPHATE-CALCIUM ION COMPLEX AT 1.5-ANGSTROMS RESOLUTION Deposited 1982-05-14 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Not recorded | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.50 Å |
| 2SOB SN-OB, OB-FOLD SUB-DOMAIN OF STAPHYLOCOCCAL NUCLEASE, NMR, 10 STRUCTURES Deposited 1995-09-15 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–185（103 aa）
|
Mutation：V66L, G88V, DEL(104-109) | No recorded non-water small molecule | SOLUTION NMR mmCIF provides none of the parsed conditions | Resolution not provided |
| 3BDC Crystal structure of Staphylococcal nuclease variant Delta+PHS at cryogenic temperature Deposited 2007-11-14 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：G50F/V51N/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；17% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 1.80 Å R-free 0.251 |
| 3C1E Crystal structure of Staphylococcal nuclease variant Delta+PHS L125K at cryogenic temperature Deposited 2008-01-23 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：G50F, V51N, P117G, L125K, H124L, S128A | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；32% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 9.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 1.90 Å R-free 0.253 |
| 3C1F Crystal structure of Staphylococcal nuclease variant Delta+PHS V104K at cryogenic temperature Deposited 2008-01-23 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：G50F, V51N, V104K, P117G, H124L, S128A | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；40% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.261 |
| 3D4D Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91E at cryogenic temperature Deposited 2008-05-14 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：G50F,V51N,Y91E,P117G,H124L,S128A,Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；25% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 2.10 Å R-free 0.290 |
| 3D4D Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91E at cryogenic temperature Deposited 2008-05-14 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
83–231（149 aa）
|
Mutation：G50F,V51N,Y91E,P117G,H124L,S128A,Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；25% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 2.10 Å R-free 0.290 |
| 3D6C Crystal structure of Staphylococcal nuclease variant PHS L38E at cryogenic temperature Deposited 2008-05-19 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：sequence database residues 83-231
|
Mutation：L38E,P117G,H124L,S128A | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；35% MPD, 25 mM Potassium Phosphate, Calcium chloride, PTP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.234 |
| 3DHQ Crystal structure of Staphylococcal nuclease variant Delta+PHS A90R at cryogenic temperature Deposited 2008-06-18 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：nuclease
|
Mutation：G50F/V51N/A90R/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；24% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTP, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.15 Å R-free 0.276 |
| 3DMU Crystal structure of Staphylococcal nuclease variant PHS T62K at cryogenic temperature Deposited 2008-07-01 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：T62K/P117G/H124L/S128A | PO4 PHOSPHATE ION × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；44% MPD, 25 mM Potassium Phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.255 |
| 3E5S Crystal structure of Staphylococcal nuclease variant Delta+PHS L103K at cryogenic temperature Deposited 2008-08-14 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：G50F/V51N/L103K/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；35% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.224 |
| 3E5S Crystal structure of Staphylococcal nuclease variant Delta+PHS L103K at cryogenic temperature Deposited 2008-08-14 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
83–231（149 aa）
|
Mutation：G50F/V51N/L103K/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；35% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.224 |
| 3ERO Crystal structure of Staphylococcal nuclease variant Delta+PHS I72E at cryogenic temperature Deposited 2008-10-02 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：G50F/V51N/I72E/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, Soaked in higher % MPD before freezing, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.85 Å R-free 0.226 |
| 3ERQ Crystal structure of Staphylococcal nuclease variant L25K at cryogenic temperature Deposited 2008-10-02 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：L25K/G50F/V51N/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；22% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, Soaked in higher % MPD before freezing, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.10 Å R-free 0.278 |
| 3EVQ Crystal structure of Staphylococcal nuclease variant Delta+PHS L25E at cryogenic temperature Deposited 2008-10-13 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：L25E/G50F/V51N/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, Soaked in higher % MPD before freezing, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.258 |
| 3H6M Crystal structure of Staphylococcal nuclease variant Delta+PHS V104E at cryogenic temperature Deposited 2009-04-23 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：44-49 deletion, G50F, V51N, V104E, P117G, H124L, S128A | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；30% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.70 Å R-free 0.222 |
| 3HEJ Crystal structure of Staphylococcal nuclease variant Delta+PHS T62R at cryogenic temperature Deposited 2009-05-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
83–231（149 aa）
Chain D
83–231（149 aa）
|
Mutation：G50F, V51N, T62R, P117G, H124L, S128A, Del44-49 Mutation：G50F, V51N, T62R, P117G, H124L, S128A, Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；24% MPD, 25 mM Potassium Phosphate, Calcium Chloride, 5% Glycerol, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 1.80 Å R-free 0.244 |
| 3HEJ Crystal structure of Staphylococcal nuclease variant Delta+PHS T62R at cryogenic temperature Deposited 2009-05-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
83–231（149 aa）
Chain C
83–231（149 aa）
|
Mutation：G50F, V51N, T62R, P117G, H124L, S128A, Del44-49 Mutation：G50F, V51N, T62R, P117G, H124L, S128A, Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 2 GOL GLYCEROL × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；24% MPD, 25 mM Potassium Phosphate, Calcium Chloride, 5% Glycerol, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 1.80 Å R-free 0.244 |
| 3HZX Crystal Structure of Staphylococcal nuclease variant D+PHS/V66K at pH 9 determined at 100 K Deposited 2009-06-24 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：G50F/V51N/V66K/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；39 % MPD, 25 mM Potassium phosphate, pdTp, CaCl2, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.237 |
| 3ITP Crystal structure of Staphylococcal nuclease variant Delta+PHS F34K at cryogenic temperature Deposited 2009-08-28 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：F34K, G50F, V51N, P117G, H124L, S128A, Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；29%(w/v) MPD, 25 mM Potassium Phosphate, Calcium Chloride (3 M eq), pdTp (2 M eq), 5.5 (mg protein)/mL in stock solution; 4uL protein stock and 4uL well solution per drop; 1-2 weeks of growth, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.75 Å R-free 0.235 |
| 3LX0 Crystal structure of Staphylococcal nuclease variant Delta+PHS D21N at cryogenic temperature Deposited 2010-02-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：residues 83-231, Deletion 126-131
|
Mutation：D103N,G132F,V133N,P117G,H199L,S210A | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；38% MPD, 25mM Potassium Phosphate, Calcium Chloride, pdTp, pH 6.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 1.50 Å R-free 0.222 |
| 3MEH Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A at cryogenic temperature Deposited 2010-03-31 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Deletion UNP residues 126-131
|
Mutation：G50F,V51N,I92A,P117G,H124L,S128A | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 1.50 Å R-free 0.234 |
| 3MHB Crystal structure of Staphylococcal nuclease variant Delta+PHS L38A at cryogenic temperature Deposited 2010-04-07 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Deletion UNP residues 126-131
|
Mutation：L38A,G50F,V51N,P117G,H124L,S128A | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；25% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.55 Å R-free 0.238 |
| 3MVV Crystal structure of Staphylococcal nuclease variant Delta+PHS F34A at cryogenic temperature Deposited 2010-05-04 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Deletion UNP residues 126-131
|
Mutation：F34A,G132F,V133N,P199G,S210A | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；25% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 9.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 1.72 Å R-free 0.230 |
| 3MXP Crystal structure of Staphylococcal nuclease variant Delta+PHS T62A at cryogenic temperature Deposited 2010-05-07 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Deletion UNP residues 126-131
|
Mutation：G50F,V51N,T62A,P117G,H124L,S128A | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；25% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.61 Å R-free 0.247 |
| 3MZ5 Crystal structure of Staphylococcal nuclease variant Delta+PHS L103A at cryogenic temperature Deposited 2010-05-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Deletion UNP residues 126-131
|
Mutation：G50F,V51N,L103A,P117G,H124L,S128A | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.58 Å R-free 0.238 |
| 3NHH Crystal structure of Staphylococcal nuclease variant V23E-L36K at cryogenic temperature Deposited 2010-06-14 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23E, L36K, G50F, V51N, P117G, H124L, S128A | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.255 |
| 3NK9 Crystal structure of Staphylococcal nuclease variant Delta+PHS V74A at cryogenic temperature Deposited 2010-06-18 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：G50F, V51N, V74A, P117G, H124L, S128A | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；22% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 1.65 Å R-free 0.224 |
| 3NP8 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36A at cryogenic temperature Deposited 2010-06-28 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Deletion UNP residues 126-131
|
Mutation：L36A,G50F,V51N,P117G,H124L,S128A | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 6.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 1.70 Å R-free 0.230 |
| 3NQT Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A at cryogenic temperature Deposited 2010-06-29 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Deletion UNP residues 126-131
|
Mutation：G50F,V51N,V66A,P117G,H124L,S128A | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；28% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 7.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 1.90 Å R-free 0.246 |
| 3NUC STAPHLOCOCCAL NUCLEASE, 1-N-PROPANE THIOL DISULFIDE TO V23C VARIANT Deposited 1997-12-06 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23C Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.15；10 MM POTASSIUM PHOSPHATE, PH 8.15, PROTEIN CONCENTRATION 2 MGS/ML, 21 PERCENT MPD AT 4C.
|
Resolution 1.90 Å |
| 3NXW Crystal structure of Staphylococcal nuclease variant Delta+PHS L125A at cryogenic temperature Deposited 2010-07-14 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Deletion UNP residues 126-131
|
Not recorded | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；29% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 9.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 1.65 Å R-free 0.238 |
| 3OSO Crystal structure of Staphylococcal nuclease variant Delta+PHS L25A at cryogenic temperature Deposited 2010-09-09 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Deletion UNP residues 126-131
|
Mutation：L25A, G132P, V133N, P199G, H206L, S210A | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 7.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 1.60 Å R-free 0.206 |
| 3OWF Crystal structure of Staphylococcal nuclease variant Delta+PHS V66R at cryogenic temperature Deposited 2010-09-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
83–231（149 aa）
|
Mutation：G50F/V51N/V66R/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 2 CA CALCIUM ION × 2 PO4 PHOSPHATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；277 K；19% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.85 Å R-free 0.213 |
| 3P1H Crystal structure of Staphylococcal nuclease variant Delta+PHS V23K/I92A at cryogenic temperature Deposited 2010-09-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Deletion UNP residues 126-131
|
Mutation：V23K,G50F,V51N,I92A,P117G,H124L,S128A | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；27% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.79 Å R-free 0.231 |
| 3P75 Crystal structure of Staphylococcal nuclease variant Delta+PHS V104D at cryogenic temperature Deposited 2010-10-12 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
89–223（135 aa）
|
Mutation：G50F/V51N/V104D/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 2 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9.5；277 K；35% MPD(w/v), 25 mM CHES, Calcium Chloride, pdTp, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.239 |
| 3PMF Crystal structure of Staphylococcal nuclease variant Delta+PHS V23A at cryogenic temperature Deposited 2010-11-16 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Deletion UNP residues 126-131
|
Mutation：V23A, G44F, V45N, P111G, H118L, S122A | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；22% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 7.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 1.60 Å R-free 0.213 |
| 3QB3 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92KL25A at cryogenic temperature Deposited 2011-01-12 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：UNP L107A,G132F,V133N,I174K,P199G,H206L,S210A, del(126-131) | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；27% MPD, 25 mM potassium phosphate, 4.5 mg/mL (0.28 mM) protein, 0.84 mM calcium chloride, 0.56 mM pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.63 Å R-free 0.208 |
| 3QOJ Cryogenic structure of Staphylococcal nuclease variant D+PHS/V23K Deposited 2011-02-10 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：V23K,G50F,V51N,P117G,H124L,S128A, del(44-49) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；24% MPD, 25 mM potassium phosphate, pdTp (2 eq.), calcium chloride (3 eq.), pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.60 Å R-free 0.228 |
| 3QOL Crystal structure of Staphylococcal nuclease variant D+PHS/V23E at pH 6 determined at 100 K Deposited 2011-02-10 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
41–189（149 aa）
Fragment：DELETION UNP RESIDUES 126-131
|
Mutation：V23E,G50F,V51N,P117G,H124L,S128A | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6；277 K；22% MPD, 25 mM Potassium Phosphate, pdTp, CaCl2, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.227 |
| 3R3O Crystal structure of Staphylococcal nuclease variant Delta+PHS T62A at cryogenic temperature and with high redundancy Deposited 2011-03-16 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：UNP G132F,V133N,T144A,P199G,H206L,S210A,del(126-131) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.223 |
| 3RUZ Crystal structure of Staphylococcal nuclease variant Delta+PHS V74K at cryogenic temperature Deposited 2011-05-05 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F,V51N,V74K,P117G,H124L,S128A, del(44-49) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.58 Å R-free 0.207 |
| 3S9W Crystal structure of Staphylococcal nuclease variant Delta+PHS M98G bound to Ca2+ and thymidine-5',3'-diphosphate at cryogenic temperature Deposited 2011-06-02 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F/V51N/M98G/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.211 |
| 3SHL Crystal structure of Staphylococcal nuclease variant Delta+PHS V74KL25A at cryogenic temperature Deposited 2011-06-16 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：L25A,G50F,V51N,V74K,P117G,H124L,S128A, del(44-49) | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.48 Å R-free 0.216 |
| 3SK4 Crystal structure of Staphylococcal nuclease variant Delta+PHS D21N/V23E at cryogenic temperature Deposited 2011-06-22 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：D21N/V23E/G50F/V51N/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.85 Å R-free 0.228 |
| 3SK5 Crystal structure of Staphylococcal nuclease variant Delta+PHS V39D at cryogenic temperature Deposited 2011-06-22 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：V39D/G50F/V51N/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；26% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.70 Å R-free 0.213 |
| 3SK6 Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23K/L36E at cryogenic temperature Deposited 2011-06-22 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：D21N/V23K/T33V/L36E/T41I/G50F/V51N/S59A/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.238 |
| 3SK8 Crystal structure of Staphylococcal nuclease variant Delta+PHS M98G apo protein at cryogenic temperature Deposited 2011-06-22 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F/V51N/M98G/P117G/H124L/S128A/Del44-49 | PO4 PHOSPHATE ION × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；34% MPD, 25 mM potassium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.210 |
| 3SR1 Crystal structure of Staphylococcal nuclease variant Delta+PHS A69G bound to Ca2+ and thymidine-5',3'-diphosphate at cryogenic temperature Deposited 2011-07-06 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F/V51N/A69G/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.45 Å R-free 0.202 |
| 3SXH Crystal structure of Staphylococcal nuclease variant Delta+PHS I92AL103A at cryogenic temperature Deposited 2011-07-14 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F,V51N,I92A,L103A,P117G,H124L,S128A, del(44-49) | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.40 Å R-free 0.199 |
| 3T13 Crystal structure of Staphylococcal nuclease variant Delta+PHS A69G at cryogenic temperature Deposited 2011-07-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F/V51N/A69G/P117G/H124L/S128A/Del44-49 | PO4 PHOSPHATE ION × 2 CA CALCIUM ION × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；41% MPD, 25 mM potassium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.199 |
| 3T13 Crystal structure of Staphylococcal nuclease variant Delta+PHS A69G at cryogenic temperature Deposited 2011-07-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F/V51N/A69G/P117G/H124L/S128A/Del44-49 | PO4 PHOSPHATE ION × 1 CA CALCIUM ION × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；41% MPD, 25 mM potassium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.199 |
| 3T16 Crystal structure of Staphylococcal nuclease variant NVIAGA/M98G at cryogenic temperature Deposited 2011-07-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：D21N/T33V/T41I/S59A/M98G/P117G/S128A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；41% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.211 |
| 3TME Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23E at cryogenic temperature Deposited 2011-08-31 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：D21N/V23E/T33V/T41I/G50F/V51N/S59A/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；38% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.40 Å R-free 0.208 |
| 3TP5 Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23E/L36E at cryogenic temperature Deposited 2011-09-07 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：D21N/V23E/T33V/L36E/T41I/G50F/V51N/S59A/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；31% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.231 |
| 3TP6 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E/L103K at cryogenic temperature Deposited 2011-09-07 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：L36E/G50F/V51N/L103K/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.217 |
| 3TP7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V99E at cryogenic temperature Deposited 2011-09-07 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F/V51N/V99E/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.246 |
| 3TP8 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E at cryogenic temperature Deposited 2011-09-07 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：L36E/G50F/V51N/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.60 Å R-free 0.212 |
| 3U9O Crystal structure of Staphylococcal nuclease variant NVIAGA/N100G at cryogenic temperature Deposited 2011-10-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：D21N/T33V/T41I/S59A/N100G/P117G/S128A | PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；40% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.95 Å R-free 0.239 |
| 3V2T Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/V66A at cryogenic temperature Deposited 2011-12-12 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F,V51N,V66A,I92A,P117G,H124L,S128A,del(44-49) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.220 |
| 3VA5 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/V23A at cryogenic temperature Deposited 2011-12-28 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：unp residues 83-231
|
Mutation：V102A, G50F,V51N,I92A,L103A,P117G,H124L,S128A, del(44-49) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.55 Å R-free 0.218 |
| 4DF7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23L/V99I at cryogenic temperature Deposited 2012-01-23 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：V23L,G50F,V51N,V99I,P117G,H124L,S128A,del(44-49) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.50 Å R-free 0.224 |
| 4DFA Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/L36A at cryogenic temperature Deposited 2012-01-23 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：L36A,G50F,V51N,I92A,P117G,H124L,S128A,del(44-49) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 1.40 Å R-free 0.188 |
| 4DGZ Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/L125A at cryogenic temperature Deposited 2012-01-27 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F,V51N,I92A,P117G,H124L,L125A,S128A,del(44-49) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.47 Å R-free 0.213 |
| 4DU9 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/V74A at cryogenic temperature Deposited 2012-02-21 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F,V51N,I92A,P117G,H124L,S128A,del(44-49) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.63 Å R-free 0.208 |
| 4E6I Crystal structure of Staphylococcal nuclease variant NVIAGA V99G at cryogenic temperature Deposited 2012-03-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：D21N/T33V/T41I/S59A/V99G/P117G/S128A | PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；35% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.268 |
| 4E6I Crystal structure of Staphylococcal nuclease variant NVIAGA V99G at cryogenic temperature Deposited 2012-03-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：D21N/T33V/T41I/S59A/V99G/P117G/S128A | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；35% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.268 |
| 4EQN Crystal structure of Staphylococcal nuclease variant Delta+PHS V23E/I72K at cryogenic temperature Deposited 2012-04-19 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：V23E/G50F/V51N/I72K/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.214 |
| 4EQO Crystal structure of Staphylococcal nuclease variant Delta+PHS V99D at cryogenic temperature Deposited 2012-04-19 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F/V51N/V99D/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.70 Å R-free 0.234 |
| 4EQP Crystal structure of Staphylococcal nuclease variant Delta+PHS I72D at cryogenic temperature Deposited 2012-04-19 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F/V51N/I72D/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.35 Å R-free 0.188 |
| 4EVO Crystal structure of Staphylococcal nuclease variant NVIAGA/E122G at cryogenic temperature Deposited 2012-04-26 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：D21N/T33V/T41I/S59A/P117G/E122G/S128A | PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；44% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.95 Å R-free 0.227 |
| 4F7X Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/L25A at cryogenic temperature Deposited 2012-05-16 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：L25A,G50F,V51N,I92A,P117G,H124L,S128A,del(44-49) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.70 Å R-free 0.197 |
| 4F8M Crystal structure of Staphylococcal nuclease variant Delta+PHS V23I/I92V at cryogenic temperature Deposited 2012-05-17 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：V23I,G50F,V51N,I92V,P117G,H124L,S128A,del(44-49) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.60 Å R-free 0.198 |
| 4G57 Staphylococcal Nuclease double mutant I72L, I92L Deposited 2012-07-17 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
89–223（135 aa）
|
Mutation：I72L, I92L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.4；277 K；57% MPD, 200 mM Tris.HCl buffer, approx. 6 mg/ml protein, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.81 Å R-free 0.232 |
| 4H7B Crystal Structure of Staphylococcal nuclease mutant I72V/V99L Deposited 2012-09-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP Residues 83-231
|
Mutation：I72V, V99L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25mM Sodium Phosphate buffer, 35-60% MPD, Vapor Diffusion, Hanging Drop, Temperature 277K, pH 7.0, VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.60 Å R-free 0.248 |
| 4HMI Crystal structure of Staphylococcal nuclease variant Delta+PHS V99K at cryogenic temperature Deposited 2012-10-18 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F/V51N/V99K/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, with calcium chloride and pdTp added to protein in a 3:2:1 ratio, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.75 Å R-free 0.221 |
| 4HMJ Crystal structure of Staphylococcal nuclease variant Delta+PHS L36D at cryogenic temperature Deposited 2012-10-18 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：L36D/G50F/V51N/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, with calcium chloride and pdTp added to protein in a 3:2:1 ratio, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.35 Å R-free 0.200 |
| 4HTH Crystal structure of Staphylococcal nuclease variant Delta+VIAGLA at cryogenic temperature Deposited 2012-11-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：T33V/T41I/G50V/V51N/S59A/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；42% MPD, 25 mM potassium phosphate, with protein:pdTp:calcium chloride in 1:2:3 molar equivalent ratio, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.75 Å R-free 0.209 |
| 4I65 Crystal Structure of Staphylococcal nuclease mutant V23I/L25V/I72V/I92V Deposited 2012-11-29 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
88–223（136 aa）
Fragment：UNP Residues 83-223
|
Mutation：V23I, L25V, I72V, I92V | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25mM Sodium Phosphate buffer, 35-60% MPD, Vapor Diffusion, Hanging Drop, Temperature 277K, pH 7.0, VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.61 Å R-free 0.239 |
| 4IAL Crystal structure of Staphylococcal nuclease variant Delta+PHS H121E at cryogenic temperature Deposited 2012-12-06 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F/V51N/P117G/H121E/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；24% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.59 Å R-free 0.239 |
| 4ID6 Crystal Structure of Staphylococcal nuclease mutant V23I/I72L Deposited 2012-12-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23I, I72L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.241 |
| 4IUN Crystal structure of Staphylococcal nuclease variant Delta+PHS R126E at cryogenic temperature Deposited 2013-01-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F/V51N/P117G/H124L/R126E/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；40% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.60 Å R-free 0.179 |
| 4IZ8 Crystal structure of Staphylococcal nuclease variant Delta+PHS H8E at cryogenic temperature Deposited 2013-01-29 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：H8E/G50F/V51N/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；23% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.70 Å R-free 0.213 |
| 4J1M Crystal structure of Staphylococcal nuclease variant Delta+PHS R105E at cryogenic temperature Deposited 2013-02-01 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F/V51N/R105E/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；27% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.65 Å R-free 0.214 |
| 4J6H Crystal structure of Staphylococcal nuclease variant Delta+PHS H121Q at cryogenic temperature Deposited 2013-02-11 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F/V51N/P117G/H121Q/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.60 Å R-free 0.205 |
| 4K14 Crystal Structure of Staphylococcal nuclease mutant V66I/V99L Deposited 2013-04-04 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
88–223（136 aa）
Fragment：UNP Residues 83-231
|
Mutation：V66I V99L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer,pH 7.5 45% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.60 Å R-free 0.252 |
| 4K2K Crystal structure of Staphylococcal nuclease variant Delta+PHS L25A/L36A/I92A at cryogenic temperature Deposited 2013-04-09 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：L25A,L36A,G50F,V51N,I92A,P117G,H124L,S128A,del(44-49) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.65 Å R-free 0.217 |
| 4K2L Crystal structure of Staphylococcal nuclease variant Delta+PHS I92Q at cryogenic temperature Deposited 2013-04-09 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50P,V51N,I92Q,P117G,H124L,S128A,del(44-49) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.55 Å R-free 0.214 |
| 4K5V Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/L36A/V99T at cryogenic temperature Deposited 2013-04-15 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：V23T,L36A,G50F,V51N,V99T,P117G,H124L,S128A,del(44-49) | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.75 Å R-free 0.221 |
| 4K5W Crystal structure of Staphylococcal nuclease variant V23M/L25F/T62F at cryogenic temperature Deposited 2013-04-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：V23M,L25F,T62F | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；32% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.65 Å R-free 0.202 |
| 4K5X Crystal structure of Staphylococcal nuclease variant V23M/L36F at cryogenic temperature Deposited 2013-04-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：V23M,L36F | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 2 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.65 Å R-free 0.214 |
| 4K6D Crystal structure of Staphylococcal nuclease variant V23M/T62F at cryogenic temperature Deposited 2013-04-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：V23M,T62F | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；34% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.65 Å R-free 0.213 |
| 4K8I Crystal Structure of Staphylococcal Nuclease mutant I92V/V99L Deposited 2013-04-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
89–223（135 aa）
Fragment：UNP Residues 89-223
|
Mutation：I174V/V191L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25mM Sodium Phosphate, MPD 35-60%, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.10 Å R-free 0.247 |
| 4K8J Crystal Structure of Staphylococcal nuclease mutant V23L/V66I Deposited 2013-04-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
89–223（135 aa）
|
Mutation：V105L/V148I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25mM Sodium Phosphate, 35-60% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.251 |
| 4KD4 Crystal structure of Staphylococcal nuclease variant V23L/L25V/V66I/I72V at cryogenic temperature Deposited 2013-04-24 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：V23L,L25V,V66I,I72V | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 1.55 Å R-free 0.219 |
| 4KHV Crystal structure of Staphylococcal nuclease variant Delta+PHS V23S at cryogenic temperature Deposited 2013-05-01 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：V23S/G50F/V51N/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；26% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.60 Å R-free 0.207 |
| 4KJN Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/V66A/V99T at cryogenic temperature Deposited 2013-05-03 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：V23T,G50F,V51N,V66A,V99T,P117G,H124L,S128A | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.55 Å R-free 0.228 |
| 4KJO Crystal structure of Staphylococcal nuclease variant Delta+PHS L36A/V66T/V99T at cryogenic temperature Deposited 2013-05-03 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：L36A, G50F,V51N,V66T,V99T,P117G,H124L,S128A | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.231 |
| 4KTA Crystal structure of Staphylococcal nuclease variant Delta+PHS R105Q at cryogenic temperatures Deposited 2013-05-20 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F/V51N/R105Q/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；29% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.95 Å R-free 0.210 |
| 4KV6 Crystal structure of Staphylococcal nuclease variant Delta+PHS R126Q at cryogenic temperature Deposited 2013-05-22 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F/V51N/P117G/H124L/Q126R/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；24% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.55 Å R-free 0.203 |
| 4KY5 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D at pH 7 and cryogenic temperature Deposited 2013-05-28 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：V23D/G50F/V51N/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；28% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.40 Å R-free 0.187 |
| 4KY6 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25D at pH 6 and cryogenic temperature Deposited 2013-05-28 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：L25D/G50F/V51N/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.70 Å R-free 0.227 |
| 4KY7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V74D at cryogenic temperature Deposited 2013-05-28 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F/V51N/V74D/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.55 Å R-free 0.207 |
| 4LAA Crystal structure of Staphylococcal nuclease variant Delta+PHS L36H at cryogenic temperature Deposited 2013-06-19 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：L36H/G50F/V51N/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.58 Å R-free 0.191 |
| 4ME5 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23S/V66A at cryogenic temperature Deposited 2013-08-25 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：V23S, G50F,V51N,V66A,P117G,H124L,S128A | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.223 |
| 4MIU Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/V99T at cryogenic temperature Deposited 2013-09-02 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：V23T, G50F,V51N,V99T,P117G,H124L,S128A,del(44-49) | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.67 Å R-free 0.235 |
| 4N9P Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/L25A/V99T at cryogenic temperature Deposited 2013-10-21 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：V23T,L25A,G50F,V51N,V99T,P117G,H124L,S128A,del(44-49) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.40 Å R-free 0.196 |
| 4N9T Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A/I92S at cryogenic temperature Deposited 2013-10-21 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F,V51N,V66A,I92S,P117G,H124L,S128A,del(44-49) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.60 Å R-free 0.223 |
| 4NDX Crystal structure of Staphylococcal nuclease variant Delta+PHS I92N at cryogenic temperature Deposited 2013-10-28 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F,V51N,I92N,P117G,H124L,S128A,del(44-49) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.50 Å R-free 0.216 |
| 4NKL Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A/I92Q at cryogenic temperature Deposited 2013-11-12 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F,V51N,V66A,I92Q,P117G,H124L,S128A,del(44-49) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.60 Å R-free 0.219 |
| 4NMZ Crystal structure of Staphylococcal nuclease variant Delta+PHS V23Q/V66A at cryogenic temperature Deposited 2013-11-15 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：V23Q,G50F,V51N,V66A,P117G,H124L,S128A,del(44-49) | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.70 Å R-free 0.212 |
| 4NP5 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A/I92N at cryogenic temperature Deposited 2013-11-20 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F,V51N,V66A,I92N,P117G,H124L,S128A,del(44-49) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.50 Å R-free 0.214 |
| 4ODG Crystal structure of Staphylococcal nuclease variant V23I/V66I/V74I/V99I at cryogenic temperature Deposited 2014-01-10 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：V23I/V66I/V74I/V99I | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；24% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.73 Å R-free 0.240 |
| 4OL7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66E A109E at cryogenic temperature Deposited 2014-01-23 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F/V51N/V66E/A109E/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.67 Å R-free 0.191 |
| 4OL7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66E A109E at cryogenic temperature Deposited 2014-01-23 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F/V51N/V66E/A109E/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.67 Å R-free 0.191 |
| 4PMB Crystal structure of Staphylcoccal nuclease variant Delta+PHS I92S at cryogenic temperature Deposited 2014-05-20 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：Delta 44-49, G50F, V51N, I92S, P117G, H124L, S128A | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；25% MPD, 25mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.80 Å R-free 0.227 |
| 4PMC Crystal structure of Staphylcoccal nuclease variant Delta+PHS I72K V74K at cryogenic temperature Deposited 2014-05-20 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：Delta 44-49, G50F, V51N, I72K, V74K, P117G, H124L, S128A | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25% MPD, 25mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.65 Å R-free 0.210 |
| 4PNY Crystal structure of Staphylococcal nuclease variant Delta+PHS I72H at cryogenic temperature Deposited 2014-02-22 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：G50F/V51N/I72H/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.68 Å R-free 0.242 |
| 4QB4 Crystal Structure of Staphylococcal Nuclease mutant V23L/L25V/V66L Deposited 2014-05-06 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
88–223（136 aa）
Fragment：UNP Residues 88-223
|
Mutation：V23L, L25V, V66L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；0.6 M Ammonium sulfate, MPD 50-60%, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K, temperature 277.0K
|
Resolution 1.60 Å R-free 0.236 |
| 4QF4 Crystal structure of Staphylococcal nuclease variant V23M at cryogenic temperature Deposited 2014-05-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
83–231（149 aa）
Chain B
83–231（149 aa）
|
Mutation：V23M Mutation：V23M | THP THYMIDINE-3',5'-DIPHOSPHATE × 2 CA CALCIUM ION × 2 PO4 PHOSPHATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；32% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 6.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 1.80 Å R-free 0.239 |
| 4QF4 Crystal structure of Staphylococcal nuclease variant V23M at cryogenic temperature Deposited 2014-05-19 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
83–231（149 aa）
|
Mutation：V23M | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；32% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 6.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 1.80 Å R-free 0.239 |
| 4QF4 Crystal structure of Staphylococcal nuclease variant V23M at cryogenic temperature Deposited 2014-05-19 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23M | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；32% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 6.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 1.80 Å R-free 0.239 |
| 4R8N Crystal structure of Staphylococcal nuclease variant V23I/V66I/I72V/I92V at cryogenic temperature Deposited 2014-09-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–223（141 aa）
Fragment：UNP residues 83-223
|
Not recorded | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 PO4 PHOSPHATE ION × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.65 Å R-free 0.239 |
| 4RKB Crystal structure of Staphylococcal nuclease variant Delta+PHS V66T/V99T at cryogenic temperature Deposited 2014-10-12 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-232
|
Not recorded | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；30% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp , pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.88 Å R-free 0.225 |
| 4RKL Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/V66T at cryogenic temperature Deposited 2014-10-13 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-232
|
Mutation：V23T, V60T | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K
|
Resolution 1.66 Å R-free 0.232 |
| 4S3S Crystal structure of Staphylococcal nuclease variant Delta+PHS I92K/V23A at cryogenic temperature Deposited 2015-06-19 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：Nuclease A (UNP residues 83-231)
|
Mutation：V23A/G50F/I92K/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.64 Å R-free 0.216 |
| 4TRD Crystal structure of Staphylcoccal nuclease variant Delta+PHS I72K/V74K at pH 9.0 at cryogenic temperature Deposited 2014-06-16 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：fragment 83-231
|
Mutation：Delta 44-49, G50F, V51N, I72K, V74K, P117G, H124L, S128A | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.60 Å R-free 0.195 |
| 4WOR Staphylococcal nuclease in complex with Ca2+ and thymidine-3'-5'-diphosphate (pdTp) at room temperature Deposited 2014-10-16 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Not recorded | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.15；277 K；2-methyl-2,4-pentanediol (MPD), potassium phosphate, thymidine-3',5'-diphosphate (pdTp), Ca2+
|
Resolution 1.60 Å R-free 0.204 |
| 4YIJ Crystal structure of Staphylcoccal nuclease variant Delta+PHS A109E at cryogenic temperature Deposited 2015-03-02 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：G50F/V51N/A109E/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；25% MPD, 25mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.64 Å R-free 0.190 |
| 4YIJ Crystal structure of Staphylcoccal nuclease variant Delta+PHS A109E at cryogenic temperature Deposited 2015-03-02 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
83–231（149 aa）
|
Mutation：G50F/V51N/A109E/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；25% MPD, 25mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.64 Å R-free 0.190 |
| 4ZQ3 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92T at cryogenic temperature Deposited 2015-05-08 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：G50F/V51N/I92T/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；35% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.90 Å R-free 0.254 |
| 4ZUI Crystal structure of Staphylococcal nuclease variant Delta+PHS V23H at cryogenic temperature Deposited 2015-05-16 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23H/G50F/V51N/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.70 Å R-free 0.250 |
| 4ZUJ Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91H at cryogenic temperature Deposited 2015-05-16 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：G50F/V51N/Y91H/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.72 Å R-free 0.207 |
| 5C3W Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H at cryogenic temperature Deposited 2015-06-17 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/V66H/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.72 Å R-free 0.207 |
| 5C3X Crystal structure of Staphylococcal nuclease variant Delta+PHS I72D/N100E at cryogenic temperature Deposited 2015-06-17 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/I72D/N100E/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.95 Å R-free 0.208 |
| 5C4H Crystal structure of Staphylococcal nuclease variant Delta+PHS I92H at cryogenic temperature Deposited 2015-06-18 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/I92H/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.80 Å R-free 0.196 |
| 5C4Z Crystal structure of Staphylococcal nuclease variant Delta+PHS L25H at cryogenic temperature Deposited 2015-06-18 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：L25H/G50F/V51N/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.62 Å R-free 0.192 |
| 5C5L Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E / L125E at cryogenic temperature Deposited 2015-06-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/T62E/P117G/H124L/L125E/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；35% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.75 Å R-free 0.191 |
| 5C5M Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D / V104E at cryogenic temperature Deposited 2015-06-20 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/T62D/V104E/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.70 Å R-free 0.213 |
| 5C6A Crystal structure of Staphylococcal nuclease variant Delta+PHS L25E / N100E at cryogenic temperature Deposited 2015-06-22 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：L25E/G50F/V51N/N100E/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.85 Å R-free 0.203 |
| 5CC4 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E/V66E at cryogenic temperature Deposited 2015-07-01 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/T62E/V66E/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.90 Å R-free 0.227 |
| 5CGK Crystal structure of Staphylococcal nuclease variant Delta+PHS V66D/N100E at cryogenic temperature Deposited 2015-07-09 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/V66D/N100E/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.85 Å R-free 0.239 |
| 5CR3 Crystal structure of Staphylococcal nuclease variant Delta+PHS V104E/L125E at cryogenic temperature Deposited 2015-07-22 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：K16A,G50P,V51N,V104E,P117G,H124L,L125E,S128A,del(44-49) | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.80 Å R-free 0.232 |
| 5CV4 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D/V66H at cryogenic temperature Deposited 2015-07-25 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23D/G50F/V51N/V66H/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.80 Å R-free 0.232 |
| 5CV5 Crystal structure of Staphylococcal nuclease variant Delta+PHS K64G/V66K/E67G at cryogenic temperature Deposited 2015-07-25 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：G50F/V51N/K64G/V66K/E67G/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.80 Å R-free 0.225 |
| 5CV5 Crystal structure of Staphylococcal nuclease variant Delta+PHS K64G/V66K/E67G at cryogenic temperature Deposited 2015-07-25 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
83–231（149 aa）
|
Mutation：G50F/V51N/K64G/V66K/E67G/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.80 Å R-free 0.225 |
| 5CV6 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I92E at cryogenic temperature Deposited 2015-07-25 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：G50F/V51N/V66H/I92E/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.95 Å R-free 0.232 |
| 5CV7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I92D at cryogenic temperature Deposited 2015-07-25 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：G50F/V51N/V66H/I92D/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.65 Å R-free 0.205 |
| 5CV8 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D/T62H at cryogenic temperature Deposited 2015-07-25 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23D/G50F/V51N/T62H/P117G/H124L/S128A/del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.58 Å R-free 0.198 |
| 5CV9 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E/V66H at cryogenic temperature Deposited 2015-07-25 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：L36E/G50F/V51N/V66H/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.62 Å R-free 0.220 |
| 5DAU Crystal structure of Staphylococcal nuclease variant Delta+PHS V39E/V104E at cryogenic temperature Deposited 2015-08-20 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 803-231
|
Mutation：V39E/G50F/V51N/V104E/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.50 Å R-free 0.190 |
| 5DEH Crystal structure of Staphylococcal nuclease variant Delta+PHS N100D at cryogenic temperature Deposited 2015-08-25 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/N100D/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.70 Å R-free 0.200 |
| 5E1F Crystal structure of Staphylococcal nuclease variant Delta+PHS G20E at cryogenic temperature Deposited 2015-09-29 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/I92K/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；35% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.90 Å R-free 0.228 |
| 5E3F Crystal structure of Staphylococcal nuclease variant Delta+PHS I92K at cryogenic temperature Deposited 2015-10-02 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：G50F/V51N/I92K/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；26% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.75 Å R-free 0.224 |
| 5EGT Crystal structure of Staphylococcal nuclease variant Delta+PHS V66E at cryogenic temperature Deposited 2015-10-27 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/V66E/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.85 Å R-free 0.218 |
| 5EKK Crystal structure of Staphylococcal nuclease variant Delta+PHS V39D/L125E at cryogenic temperature Deposited 2015-11-03 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V39D/V51N/P117G/H124L/L125E/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 2.10 Å R-free 0.238 |
| 5EKL Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D/N100E at cryogenic temperature Deposited 2015-11-03 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/T62D/N100E/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 2.10 Å R-free 0.244 |
| 5F2D Crystal structure of Staphylococcal nuclease variant Delta+PHS I92N/V99T at cryogenic temperature Deposited 2015-12-01 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/I92N/V99T/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.70 Å R-free 0.222 |
| 5HGT Crystal structure of Staphylococcal nuclease variant Delta+PHS L25K/I92T at cryogenic temperature Deposited 2016-01-08 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：L25K/G50F/V51N/I92T/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.75 Å R-free 0.212 |
| 5HUR Crystal structure of Staphylococcal nuclease variant Delta+PHS L25T/I92K at cryogenic temperature Deposited 2016-01-27 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：L25T/G50F/V51N/I92K/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.50 Å R-free 0.231 |
| 5I6W Crystal structure of Staphylococcal nuclease variant Delta+PHS A58D at cryogenic temperature Deposited 2016-02-16 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 80-228
|
Mutation：G50F/V51N/A58D/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；18% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.90 Å R-free 0.235 |
| 5I6Y Crystal structure of Staphylococcal nuclease variant Delta+PHS A90E at cryogenic temperature Deposited 2016-02-16 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 80-228
|
Mutation：G50F/V51N/A90E/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.90 Å R-free 0.222 |
| 5I9O Crystal structure of Staphylococcal nuclease variant Delta+PHS L36K L103K at cryogenic temperature Deposited 2016-02-20 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：L36K/G50F/V51N/L103K/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.95 Å R-free 0.238 |
| 5I9O Crystal structure of Staphylococcal nuclease variant Delta+PHS L36K L103K at cryogenic temperature Deposited 2016-02-20 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：L36K/G50F/V51N/L103K/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.95 Å R-free 0.238 |
| 5I9P Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H at cryogenic temperature Deposited 2016-02-20 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/T62H/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；19% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.85 Å R-free 0.228 |
| 5IGB Crystal structure of Staphylococcal nuclease variant Delta+PHS L36D/V66H at cryogenic temperature Deposited 2016-02-27 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：L36D/G50F/V51N/V66H/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.80 Å R-free 0.226 |
| 5IGC Crystal structure of Staphylococcal nuclease variant Delta+PHS V23E/V66H at cryogenic temperature Deposited 2016-02-27 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：V23E/G50F/V51N/V66H/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.80 Å R-free 0.225 |
| 5IGD Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/I92E at cryogenic temperature Deposited 2016-02-27 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/T62H/I92E/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.80 Å R-free 0.234 |
| 5IGE Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/V99D at cryogenic temperature Deposited 2016-02-27 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/T62H/V99D/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；28% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.80 Å R-free 0.211 |
| 5IGF Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/V66D at cryogenic temperature Deposited 2016-02-27 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/T62H/V66D/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.70 Å R-free 0.221 |
| 5IGG Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I72E at cryogenic temperature Deposited 2016-02-27 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/V66H/I72E/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.70 Å R-free 0.237 |
| 5IIF Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D at cryogenic temperature Deposited 2016-03-01 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/T62D/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.85 Å R-free 0.238 |
| 5IOC Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/V99D at cryogenic temperature Deposited 2016-03-08 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/V66H/V99D/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；24% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.90 Å R-free 0.235 |
| 5IOD Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/V99E at cryogenic temperature Deposited 2016-03-08 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/V66H/V99E/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.60 Å R-free 0.231 |
| 5ISR Crystal structure of Staphylococcal nuclease variant Delta+PHS L38E at cryogenic temperature Deposited 2016-03-15 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：L38E/G50F/V51N/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.90 Å R-free 0.236 |
| 5J1Z Crystal structure of Staphylococcal nuclease variant Delta+PHS A90D at cryogenic temperature Deposited 2016-03-29 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/A90D/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.73 Å R-free 0.209 |
| 5J22 Crystal structure of Staphylococcal nuclease variant Delta+PHS L103D at cryogenic temperature Deposited 2016-03-29 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/L103D/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.90 Å R-free 0.235 |
| 5JAV Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91D at cryogenic temperature Deposited 2016-04-12 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/Y91D/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；26% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.90 Å R-free 0.245 |
| 5JOB Crystal structure of Staphylococcal nuclease variant Delta+PHS L25K/I92A at cryogenic temperature Deposited 2016-05-02 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：L25K/G50F/V51N/I92A/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.45 Å R-free 0.182 |
| 5K5P Crystal structure of Staphylococcal nuclease variant Delta+PHS I72K L103K at cryogenic temperature Deposited 2016-05-23 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/I72K/L103K/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.83 Å R-free 0.237 |
| 5KEE Crystal structure of Staphylococcal nuclease variant Delta+PHS L25K/I92F at cryogenic temperature Deposited 2016-06-09 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：L25K/G50F/V51N/I92F/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.50 Å R-free 0.203 |
| 5KGU Crystal structure of Staphylococcal nuclease variant Delta+PHS N118D at cryogenic temperature Deposited 2016-06-13 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/P117G/N118D/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.90 Å R-free 0.233 |
| 5KIX Crystal structure of Staphylococcal nuclease variant Delta+PHS I92E at cryogenic temperature Deposited 2016-06-17 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/I92E/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.75 Å R-free 0.223 |
| 5KRU Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E/V66K at cryogenic temperature Deposited 2016-07-07 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/T62E/V66K/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；28% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.60 Å R-free 0.221 |
| 5KYI Crystal structure of Staphylococcal nuclease variant Delta+PHS T62K/V66E pH 9 at cryogenic temperature Deposited 2016-07-21 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/T62K/V66E/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；40% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.70 Å R-free 0.203 |
| 5KYL Crystal structure of Staphylococcal nuclease variant Delta+PHS T62K/V66E pH 7 at cryogenic temperature Deposited 2016-07-21 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/T62K/V66E/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.85 Å R-free 0.220 |
| 5NUC STAPHYLOCOCCAL NUCLEASE, 1-N-PENTANE THIOL DISULFIDE TO V23C VARIANT Deposited 1998-01-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23C, 1-N-PENTANE THIOL DISULFIDE Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.15；277 K；10 MM POTASSIUM PHOSPHATE, PH 8.15 PROTEIN CONCENTRATION = 2 MGS/ML MPD =21% T=4 DEGREES C, temperature 277K
|
Resolution 2.10 Å |
| 6AMF Crystal structure of Staphylococcal nuclease variant Delta+PHS V23K/L36E at cryogenic temperature Deposited 2017-08-09 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23K/L36E/G50F/V51N/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；24% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.85 Å R-free 0.261 |
| 6B8R Crystal structure of Staphylococcal nuclease variant Delta+PHS V23K/L36Q at cryogenic temperature Deposited 2017-10-09 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
|
Mutation：V23K/L36Q/G50F/V51N/P117G/H124L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.65 Å R-free 0.216 |
| 6EEG Crystal structure of Staphylcoccal nuclease variant Delta+PHS T62E at cryogenic temperature Deposited 2018-08-14 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/T62E/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
|
Resolution 1.95 Å R-free 0.219 |
| 6EEK Crystal structure of apo Staphylcoccal nuclease variant Delta+PHS T62E/V66K, pH 7 at cryogenic temperature Deposited 2018-08-14 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/T62E/V66K/P117G/H124L/S128A/Del44-49 | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM HEPES, 29% Jeffamine ED-2001
|
Resolution 2.20 Å R-free 0.309 |
| 6OK8 Crystal structure of Staphylococcal nuclease variant Delta+PHS K127L at cryogenic temperature Deposited 2019-04-12 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/P117G/H124L/K127L/S128A/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；46% MPD, 25 mM potassium phosphate, calcium chloride, THP
|
Resolution 1.80 Å R-free 0.225 |
| 6U0W Crystal structure of Staphylococcal nuclease variant Delta+PHS K133M at cryogenic temperature Deposited 2019-08-15 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F,V51N,P117G,H124L,S128A,K133M,Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；22% MPD, 25 mM potassium phosphate, calcium chloride, THP
|
Resolution 1.90 Å R-free 0.273 |
| 6U0X Crystal structure of Staphylococcal nuclease variant Delta+PHS Q123D at cryogenic temperature Deposited 2019-08-15 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F,V51N,P117G,Q123D,H124L,S128A,Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, THP
|
Resolution 1.86 Å R-free 0.272 |
| 6XSB Crystal structure of Staphylococcal nuclease variant Delta+PHS A132S at cryogenic temperature Deposited 2020-07-15 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/P117G/H124L/S128A/A132S/Del44-49 | CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, THP
|
Resolution 1.95 Å R-free 0.259 |
| 6XSC Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T at cryogenic temperature Deposited 2020-07-15 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：V23T/G50F/V51N/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride
|
Resolution 2.00 Å R-free 0.259 |
| 6XSE Crystal structure of Staphylococcal nuclease variant Delta+PHS F34Y at cryogenic temperature Deposited 2020-07-15 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：F34Y/G50F/V51N/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；26% MPD, 25 mM potassium phosphate, calcium chloride, THP
|
Resolution 2.10 Å R-free 0.279 |
| 6XSF Crystal structure of Staphylococcal nuclease variant Delta+PHS T41V at cryogenic temperature Deposited 2020-07-15 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；35% MPD, 25 mM potassium phosphate, calcium chloride, THP
|
Resolution 1.60 Å R-free 0.227 |
| 6XSG Crystal structure of Staphylococcal nuclease variant Delta+PHS V66T at cryogenic temperature Deposited 2020-07-15 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/V66T/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；32% MPD, 25 mM potassium phosphate, calcium chloride, THP
|
Resolution 2.00 Å R-free 0.247 |
| 6XSH Crystal structure of Staphylococcal nuclease variant Delta+PHS T62V at cryogenic temperature Deposited 2020-07-15 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/T62V/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, THP
|
Resolution 2.00 Å R-free 0.276 |
| 6XSI Crystal structure of Staphylococcal nuclease variant Delta+PHS V104T at cryogenic temperature Deposited 2020-07-15 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
83–231（149 aa）
Fragment：UNP residues 83-231
|
Mutation：G50F/V51N/V104T/P117G/H124L/S128A/Del44-49 | THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, THP
|
Resolution 2.00 Å R-free 0.271 |
| 8K2R The structure of HtpG M domain in complex with unstructured D131D binding site b Deposited 2023-07-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
152–222（71 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；310 K；Ionic strength (raw mmCIF value) 150；Pressure 1
NMR sample composition
500 uM [U-100% 15N] Heat shock protein 90 (Hsp90) M domain, 500 uM [U-10% 13C; U-99% 15N] Heat shock protein 90 (Hsp90) M domain, 500 uM [U-13C; U-15N; U-2H] Heat shock protein 90 (Hsp90) M domain, 500 uM [U-100% 13C; U-100% 15N] Disordered protein (D131D), 93% H2O/7% D2O | 93% H2O/7% D2O
|
Resolution not provided |
| 8K2T Solution structure of full-length HtpG in complex with D131D Deposited 2023-07-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
95–222（128 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；310 K；Ionic strength (raw mmCIF value) 150；Pressure 1
NMR sample composition
500 uM [U-13C; U-15N; U-2H] Heat shock protein 90(HtpG M domain), 500 uM [U-2H, 15N; Ala- 13CH3; Met- 13CH3; Ile-d1- 13CH3; Leu, Val- 13CH3/ 13CH3; Thr- 13CH3] Heat shock protein 90(HtpG M domian), 500 uM [U-13C; U-15N; U-2H] Heat shock protein 90(HtpG N domain), 500 uM [U-100% 13C; U-100% 15N] Disordered protein(D131D), 500 uM [U-2H, 15N; Ala- 13CH3; Met- 13CH3; Ile-d1- 13CH3; Leu, Val- 13CH3/ 13CH3; Thr- 13CH3] Heat shock protein 90(HtpG N domain), 600 uM [U-2H, 15N; Ala- 13CH3; Met- 13CH3; Ile-d1- 13CH3; Leu, Val- 13CH3/ 13CH3; Thr- 13CH3] Heat shock protein 90(HtpG C domain), 500 uM [U-2H, 15N; Ala- 13CH3; Met- 13CH3; Ile-d1- 13CH3; Leu, Val- 13CH3/ 13CH3; Thr- 13CH3] Heat shock protein 90(HtpG-D131D), 500 uM [U-2H, 15N; Ala- 13CH3; Met- 13CH3; Ile-d1- 13CH3; Leu, Val- 13CH3/ 13CH3; Thr- 13CH3] Heat shock protein 90(HtpG M-D131D N), 500 uM [U-2H, 15N; Ala- 13CH3; Met- 13CH3; Ile-d1- 13CH3; Leu, Val- 13CH3/ 13CH3; Thr- 13CH3] Heat shock protein 90(HtpG M-D131D C), 93% H2O/7% D2O | 93% H2O/7% D2O
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Resolution not provided |
| 9C11 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36R at cryogenic temperature Deposited 2024-05-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
83–231（149 aa）
Chain B
83–231（149 aa）
|
Not recorded | THP THYMIDINE-3',5'-DIPHOSPHATE × 2 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；28% MPD
25mM K Phosphate
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Resolution 1.95 Å R-free 0.261 |
298 other PDB entries and 310 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | NUC_STAAU |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–143； UniProt 83–231 |
