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8k2r

The structure of HtpG M domain in complex with unstructured D131D binding site b

Method: SOLUTION NMR Dmax: 64.0 Å Quality: REASONABLE
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Molecular chaperone HtpG (Fragment)

Escherichia coli

UniProt A0A7A6VTW3

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain A； UniProt 230–494 Fragment：Heat shock protein 90 (Hsp90) M domain Disordered protein(D131D) × 1 （P00644） SOLUTION NMR NMR measurement conditions：pH 7；310 K；Ionic strength (raw mmCIF value) 150；Pressure 1 NMR sample composition：500 uM [U-100% 15N] Heat shock protein 90 (Hsp90) M domain, 500 uM [U-10% 13C; U-99% 15N] Heat shock protein 90 (Hsp90) M domain, 500 uM [U-13C; U-15N; U-2H] Heat shock protein 90 (Hsp90) M domain, 500 uM [U-100% 13C; U-100% 15N] Disordered protein (D131D), 93% H2O/7% D2O | 93% H2O/7% D2O Resolution not provided

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8K2R Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
8K2S The structure of HtpG M domain in complex with unstructured D131D binding site a Deposited 2023-07-13 Different construct Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 230–494（265 aa） Fragment：Heat shock protein 90(HtpG) M domain
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7；310 K；Ionic strength (raw mmCIF value) 150；Pressure 1
NMR sample composition 500 uM [U-15N] Heat shock protein 90(HtpG) M domain, 500 uM [U-13C; U-15N; U-2H] Heat shock protein 90(HtpG) M domain, 500 uM [U-100% 13C; U-100% 15N; U-80% 2H] Heat shock protein 90(HtpG) M domain, 500 uM [U-100% 13C; U-100% 15N] Disordered protein (D131D), 93% H2O/7% D2O | 93% H2O/7% D2O
Resolution not provided

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name A0A7A6VTW3_ECOLX
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 4–268； UniProt 230–494

Disordered protein(D131D)

Staphylococcus aureus

UniProt P00644

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain B； UniProt 152–222 Not recorded Molecular chaperone HtpG (Fragment) × 1 （A0A7A6VTW3） SOLUTION NMR NMR measurement conditions：pH 7；310 K；Ionic strength (raw mmCIF value) 150；Pressure 1 NMR sample composition：500 uM [U-100% 15N] Heat shock protein 90 (Hsp90) M domain, 500 uM [U-10% 13C; U-99% 15N] Heat shock protein 90 (Hsp90) M domain, 500 uM [U-13C; U-15N; U-2H] Heat shock protein 90 (Hsp90) M domain, 500 uM [U-100% 13C; U-100% 15N] Disordered protein (D131D), 93% H2O/7% D2O | 93% H2O/7% D2O Resolution not provided

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8K2R Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1A2T STAPHYLOCOCCAL NUCLEASE, B-MERCAPTOETHANOL DISULFIDE TO V23C VARIANT Deposited 1998-01-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23C, B-MERCAPTOETHANOL DISULFIDE Non-standard monomer：Yes (specific site not provided by mmCIF) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.15；277 K；10 MM POTASSIUM PHOSPHATE, PH 8.15 PROTEIN CONCENTRATION = 2 MGS/ML MPD =21% T=4 DEGREES C, temperature 277K
Resolution 1.96 Å
1A2U STAPHYLOCOCCAL NUCLEASE, V23C VARIANT, COMPLEX WITH 1-N-BUTANE THIOL AND 3',5'-THYMIDINE DIPHOSPHATE Deposited 1998-01-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23C, S-THIOBUTYL DISULFIDE Non-standard monomer：Yes (specific site not provided by mmCIF) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.15；277 K；10 MM POTASSIUM PHOSPHATE, PH 8.15 PROTEIN CONCENTRATION = 2 MGS/ML MPD =21% T=4 DEGREES C, temperature 277K
Resolution 2.00 Å
1A3T STAPHYLOCOCCAL NUCLEASE, V23C VARIANT, COMPLEX WITH 2-FLUOROETHANE THIOL AND 3',5'-THYMIDINE DIPHOSPHATE Deposited 1998-01-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23C, S-THIOETHYLFLUORO DISULFIDE Non-standard monomer：Yes (specific site not provided by mmCIF) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.15；277 K；10 MM POTASSIUM PHOSPHATE, PH 8.15 PROTEIN CONCENTRATION = 2 MGS/ML MPD =21% T=4 DEGREES C, temperature 277K
Resolution 2.10 Å
1A3U STAPHYLOCOCCAL NUCLEASE, CYCLOHEXANE THIOL DISULFIDE TO V23C VARIANT Deposited 1998-01-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23C, CYCLOHEXANE THIOL DISULFIDE Non-standard monomer：Yes (specific site not provided by mmCIF) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.15；277 K；10 MM POTASSIUM PHOSPHATE, PH 8.15 PROTEIN CONCENTRATION = 2 MGS/ML MPD =21% T=4 DEGREES C, temperature 277K
Resolution 2.05 Å
1A3V STAPHYLOCOCCAL NUCLEASE, CYCLOPENTANE THIOL DISULFIDE TO V23C VARIANT Deposited 1998-01-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23C, CYCLOPENTANE THIOL DISULFIDE Non-standard monomer：Yes (specific site not provided by mmCIF) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.15；277 K；10 MM POTASSIUM PHOSPHATE, PH 8.15 PROTEIN CONCENTRATION = 2 MGS/ML MPD =21% T=4 DEGREES C, temperature 277K
Resolution 2.20 Å
1AEX STAPHYLOCOCCAL NUCLEASE, METHANE THIOL DISULFIDE TO V23C VARIANT Deposited 1997-03-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23C Non-standard monomer：Yes (specific site not provided by mmCIF) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.15；10 MM POTASSIUM PHOSPHATE, PH = 8.15, PROTEIN CONC. = 2 MGS/ML, MPD = 21%, T = 4 C
Resolution 2.10 Å
1ENA CRYSTAL STRUCTURES OF THE BINARY CA2+ AND PDTP COMPLEXES AND THE TERNARY COMPLEX OF THE ASP 21->GLU MUTANT OF STAPHYLOCOCCAL NUCLEASE. IMPLICATIONS FOR CATALYSIS AND LIGAND BINDING Deposited 1994-02-14 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 89–223（135 aa）
Not recorded CA CALCIUM ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.15 Å
1ENC CRYSTAL STRUCTURES OF THE BINARY CA2+ AND PDTP COMPLEXES AND THE TERNARY COMPLEX OF THE ASP 21->GLU MUTANT OF STAPHYLOCOCCAL NUCLEASE. IMPLICATIONS FOR CATALYSIS AND LIGAND BINDING Deposited 1994-02-14 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Not recorded CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.95 Å
1EQV SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE Deposited 2000-04-06 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 88–223（136 aa）
Mutation：Y113A, V114A, Y115A, K116A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions SMALL TUBES；pH 8.15；293 K；10.5 mM potassium phosphate, MPD, pH 8.15, SMALL TUBES, temperature 293K
Resolution 1.94 Å
1EY0 STRUCTURE OF WILD-TYPE S. NUCLEASE AT 1.6 A RESOLUTION Deposited 2000-05-05 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；MPD, Sodium Phosphate Buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.60 Å R-free 0.255
1EY4 STRUCTURE OF S. NUCLEASE STABILIZING MUTANT S59A Deposited 2000-05-05 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：S59A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；MPD, Sodium phosphate buffer , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.60 Å R-free 0.256
1EY5 STRUCTURE OF S. NUCLEASE STABILIZING MUTANT T33V Deposited 2000-05-05 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：T33V No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；MPD, Sodium phosphate buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.70 Å R-free 0.266
1EY6 STRUCTURE OF S. NUCLEASE STABILIZING MUTANT T41I Deposited 2000-05-05 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：T41I No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；MPD, Sodium phosphate buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.75 Å R-free 0.263
1EY7 STRUCTURE OF S. NUCLEASE STABILIZING MUTANT S128A Deposited 2000-05-05 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：S128A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；MPD, Sodium phosphate buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.88 Å R-free 0.266
1EY8 STRUCTURE OF S. NUCLEASE STABILIZING TRIPLE MUTANT P117G/H124L/S128A Deposited 2000-05-05 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：P117G,H124L,S128A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；MPD, Sodium phosphate buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.75 Å R-free 0.240
1EY9 STRUCTURE OF S. NUCLEASE STABILIZING QUADRUPLE MUTANT T41I/P117G/H124L/S128A Deposited 2000-05-05 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：T41I,P117G,H124L,S128A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；MPD, Sodium phosphate buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.72 Å R-free 0.270
1EYA STRUCTURE OF S. NUCLEASE STABILIZING QUINTUPLE MUTANT T33V/T41I/P117G/H124L/S128A Deposited 2000-05-05 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：T33V,T41I,P117G,H124L,S128A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；MPD, Sodium phosphate buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.288
1EYC STRUCTURE OF S. NUCLEASE STABILIZING QUINTUPLE MUTANT T41I/S59A/P117G/H124L/S128A Deposited 2000-05-05 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：T41I/S59A/P117G/H124L/S128A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；MPD, Sodium phosphate buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.85 Å R-free 0.250
1EYD STRUCTURE OF WILD-TYPE S. NUCLEASE AT 1.7 A RESOLUTION Deposited 2000-05-05 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；MPD, Sodium phosphate buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.70 Å R-free 0.277
1EZ6 STRUCTURE OF S. NUCLEASE STABILIZING SEXTUPLE MUTANT T33V/T41I/S59A/P117G/H124L/S128A Deposited 2000-05-10 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：T33V/T41I/S59A/P117G/H124L/S128A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；MPD, Sodium Phosphate Buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.90 Å R-free 0.279
1EZ8 STRUCTURE OF S. NUCLEASE STABILIZING MUTANT T33V Deposited 2000-05-10 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：T33V No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；MPD, Sodium phosphate buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.85 Å R-free 0.265
1F2M SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE Deposited 2000-05-26 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V114A,K116A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.15；pH 8.15
Resolution 2.00 Å
1F2Y SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE Deposited 2000-05-31 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：Y113A, K116A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions SMALL TUBES；pH 8.15；298 K；10.5 MM POTASSIUM PHOSPHATE, MPD, pH 8.15, SMALL TUBES, temperature 298K
Resolution 2.00 Å
1F2Z SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE Deposited 2000-05-31 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions SMALL TUBES；pH 8.15；298 K；10.5 MM POTASSIUM PHOSPHATE, MPD, pH 8.15, SMALL TUBES, temperature 298K
Resolution 1.80 Å
1IHZ Structure of S. nuclease mutant quintuple mutant V23L/V66L/I72L/I92L/V99L Deposited 2001-04-20 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23L, V66L, I72L, I92L, V99L No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；MPD, SODIUM PHOSPHATE BUFFER, pH 8.00, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.65 Å R-free 0.261
1II3 Structure of S. nuclease quintuple mutant V23I/V66L/I72L/I92L/V99L Deposited 2001-04-20 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23I,V66L,I72L,I92L,V99L No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；MPD, SODIUM PHOSPHATE BUFFER, pH 8.00, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.72 Å R-free 0.270
1JOK Averaged structure for Staphylococcal nuclease-H124L in ternary complex with Ca2+ and thymidine-3',5'-bisphosphate Deposited 2001-07-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：nuclease A
Mutation：H124L THP THYMIDINE-3',5'-DIPHOSPHATE × 1 SOLUTION NMR
NMR measurement conditions pH 5.5；318 K；Ionic strength (raw mmCIF value) 300 nM KCl；Pressure ambient
NMR measurement conditions pH 5.1；318 K；Ionic strength (raw mmCIF value) 300 nM KCl；Pressure ambient
NMR sample composition 3.5-5 mM Nuclease-H124L U-50% 2H; 300 mM KCl; D2O | D2O
NMR sample composition 3.5-5 mM Nuclease-H124L U-95% 15N; 300 mM KCl; H2O | H2O
NMR sample composition 3.5-5 mM Nuclease-H124L; 300 mM KCl; D2O | D2O
NMR sample composition 3.5-5 mM Nuclease-H124L U-50% 2H; 300 mM KCl; H2O | H2O
NMR sample composition 3.5-5 mM Nuclease-H124L U-98% 13C; 300 mM KCl; H2O | H2O
NMR sample composition 3.5-5 mM Nuclease-H124L U-98% 13C U-99% 15N; 300 mM KCl; H2O | H20
Resolution not provided
1JOO Averaged structure for unligated Staphylococcal nuclease-H124L Deposited 2001-07-30 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：nuclease A
Mutation：H124L No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 5.5；318 K；Ionic strength (raw mmCIF value) 300 nM KCl；Pressure ambient
NMR measurement conditions pH 5.1；318 K；Ionic strength (raw mmCIF value) 300 nM KCl；Pressure ambient
NMR sample composition 3.5-5 mM Nuclease-H124L U-50% 2H; 300 mM KCl; D2O | D2O
NMR sample composition 3.5-5 mM Nuclease-H124L U-95% 15N; 300 mM KCl; H2O | H2O
NMR sample composition 3.5-5 mM Nuclease-H124L; 300 mM KCl; D2O | D2O
NMR sample composition 3.5-5 mM Nuclease-H124L U-50% 2H; 300 mM KCl; H2O | H2O
NMR sample composition 3.5-5 mM Nuclease-H124L U-98% 13C; 300 mM KCl; H2O | H2O
NMR sample composition 3.5-5 mM Nuclease-H124L U-98% 13C U-99% 15N; 300 mM KCl; H2O | H2O
Resolution not provided
1JOQ Ensemble structures for Staphylococcal nuclease-H124L in ternary complex with Ca2+ and thymidine-3',5'-bisphosphate Deposited 2001-07-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：nuclease A
Mutation：H124L THP THYMIDINE-3',5'-DIPHOSPHATE × 1 SOLUTION NMR
NMR measurement conditions pH 5.5；318 K；Ionic strength (raw mmCIF value) 300 nM KCl；Pressure ambient
NMR measurement conditions pH 5.1；318 K；Ionic strength (raw mmCIF value) 300 nM KCl；Pressure ambient
NMR sample composition 3.5-5 mM Nuclease-H124L U-50% 2H; 300 mM KCl; D2O | D2O
NMR sample composition 3.5-5 mM Nuclease-H124L U-95% 15N; 300 mM KCl; H2O | H2O
NMR sample composition 3.5-5 mM Nuclease-H124L; 300 mM KCl; D2O | D2O
NMR sample composition 3.5-5 mM Nuclease-H124L U-50% 2H; 300 mM KCl; H2O | H2O
NMR sample composition 3.5-5 mM Nuclease-H124L U-98% 13C; 300 mM KCl; H2O | H2O
NMR sample composition 3.5-5 mM Nuclease-H124L U-98% 13C U-99% 15N; 300 mM KCl; H2O | H2O
Resolution not provided
1JOR Ensemble structures for unligated Staphylococcal nuclease-H124L Deposited 2001-07-30 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：nuclease A
Mutation：H124L No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 5.5；318 K；Ionic strength (raw mmCIF value) 300 nM KCl；Pressure ambient
NMR measurement conditions pH 5.1；318 K；Ionic strength (raw mmCIF value) 300 nM KCl；Pressure ambient
NMR sample composition 3.5-5 mM Nuclease-H124L U-50% 2H; 300 mM KCl; D2O | D2O
NMR sample composition 3.5-5 mM Nuclease-H124L U-95% 15N; 300 mM KCl; H2O | H2O
NMR sample composition 3.5-5 mM Nuclease-H124L; 300 mM KCl; D2O | D2O
NMR sample composition 3.5-5 mM Nuclease-H124L U-50% 2H; 300 mM KCl; H2O | H2O
NMR sample composition 3.5-5 mM Nuclease-H124L U-98% 13C; 300 mM KCl; H2O | H2O
NMR sample composition 3.5-5 mM Nuclease-H124L U-98% 13C U-99% 15N; 300 mM KCl; H2O | H2O
Resolution not provided
1KAA STRESS AND STRAIN IN STAPHYLOCOCCAL NUCLEASE Deposited 1992-12-18 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 88–223（136 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å
1KAB STRESS AND STRAIN IN STAPHYLOCOCCAL NUCLEASE Deposited 1992-12-18 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 88–223（136 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å
1KDA STABILIZATION OF A STRAINED PROTEIN LOOP CONFORMATION THROUGH PROTEIN ENGINEERING Deposited 1994-08-25 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å
1KDB STABILIZATION OF A STRAINED PROTEIN LOOP CONFORMATION THROUGH PROTEIN ENGINEERING Deposited 1994-08-25 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å
1KDC STABILIZATION OF A STRAINED PROTEIN LOOP CONFORMATION THROUGH PROTEIN ENGINEERING Deposited 1994-08-25 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
1NSN THE CRYSTAL STRUCTURE OF ANTIBODY N10-STAPHYLOCOCCAL NUCLEASE COMPLEX AT 2.9 ANGSTROMS RESOLUTION Deposited 1995-06-06 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain S 83–231（149 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.80 Å
1NUC STAPHYLOCOCCAL NUCLEASE, V23C VARIANT Deposited 1997-02-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23C CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.15；277 K；10 MM POTASSIUM PHOSPHATE, PH = 8.15, PROTEIN CONC. = 2 MGS/ML, MPD = 21%, T = 4 C, temperature 277K
Resolution 1.90 Å
1RKN Solution structure of 1-110 fragment of Staphylococcal Nuclease with G88W mutation Deposited 2003-11-22 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–192（110 aa） Fragment：residues 1-110
Mutation：G88W No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 4.9；305 K；Ionic strength (raw mmCIF value) 0.05；Pressure ambient
NMR sample composition 1-2mM G88W110-15N,13C labeled; 50mM Acetate buffer, DSS, NaN3; 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition 1-2mM G88W110-13C labeled; 50mM Acetate buffer, DSS, NaN3; 100% D2O | 100% D2O
NMR sample composition 1-2mM G88W110-15N labeled; 50mM Acetate buffer, DSS, NaN3; 90% H2O, 10% D2O | 90% H2O/10% D2O
Resolution not provided
1SNC THE CRYSTAL STRUCTURE OF THE TERNARY COMPLEX OF STAPHYLOCOCCAL NUCLEASE, CA2+, AND THE INHIBITOR PD*TP, REFINED AT 1.65 ANGSTROMS Deposited 1989-07-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Not recorded CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.65 Å
1SND STAPHYLOCOCCAL NUCLEASE DIMER CONTAINING A DELETION OF RESIDUES 114-119 COMPLEXED WITH CALCIUM CHLORIDE AND THE COMPETITIVE INHIBITOR DEOXYTHYMIDINE-3',5'-DIPHOSPHATE Deposited 1996-08-23 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 83–231（149 aa）
Chain B 83–231（149 aa）
Mutation：DEL(114-119) Mutation：DEL(114-119) No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.84 Å
1SND STAPHYLOCOCCAL NUCLEASE DIMER CONTAINING A DELETION OF RESIDUES 114-119 COMPLEXED WITH CALCIUM CHLORIDE AND THE COMPETITIVE INHIBITOR DEOXYTHYMIDINE-3',5'-DIPHOSPHATE Deposited 1996-08-23 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 83–231（149 aa）
Chain B 83–231（149 aa）
Mutation：DEL(114-119) Mutation：DEL(114-119) No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.84 Å
1SNM ACTIVE SITE MUTANT GLU-43 (RIGHT ARROW) ASP IN STAPHYLOCOCCAL NUCLEASE DISPLAYS NONLOCAL STRUCTURAL CHANGES Deposited 1990-02-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：D43E CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.74 Å
1SNO PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE Deposited 1996-07-06 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.15；10.5 MM POTASSIUM PHOSPHATE, 20% - 30% 2-METHYL-2,4-PENTANEDIOL, pH 8.15
Resolution 1.70 Å R-free 0.224
1SNP PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE Deposited 1996-07-06 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：P117G No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.15；10.5 MM POTASSIUM PHOSPHATE, 20% - 30% 2-METHYL-2,4-PENTANEDIOL, pH 8.15
Resolution 1.95 Å R-free 0.255
1SNQ PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE Deposited 1996-07-06 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：P47G, P117G No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.15；10.5 MM POTASSIUM PHOSPHATE, 20% - 30% 2-METHYL-2,4-PENTANEDIOL, pH 8.15
Resolution 1.70 Å R-free 0.274
1STA ACCOMMODATION OF INSERTION MUTATIONS ON THE SURFACE AND IN THE INTERIOR OF STAPHYLOCOCCAL NUCLEASE Deposited 1994-01-17 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–229（147 aa）
Not recorded CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.70 Å
1STB ACCOMMODATION OF INSERTION MUTATIONS ON THE SURFACE AND IN THE INTERIOR OF STAPHYLOCOCCAL NUCLEASE Deposited 1994-01-17 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–230（148 aa）
Not recorded CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
1STG TWO DISTINCTLY DIFFERENT METAL BINDING MODES ARE SEEN IN X-RAY CRYSTAL STRUCTURES OF STAPHYLOCOCCAL NUCLEASE-COBALT(II)-NUCLEOTIDE COMPLEXES Deposited 1994-10-27 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Not recorded CO COBALT (II) ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions COMPOUND CRYSTAL SOAKED FOR ONE WEEK AT 277 K IN STABILIZING BUFFER COMPRISING 40% (WW) MPD IN 30 MM TRIS-CL PH 8.15, 50 UM COCL2, 100 UM POTASSIUM CITRATE AND 50 UM PDTP.
Resolution 1.70 Å
1STH TWO DISTINCTLY DIFFERENT METAL BINDING MODES ARE SEEN IN X-RAY CRYSTAL STRUCTURES OF STAPHYLOCOCCAL NUCLEASE-COBALT(II)-NUCLEOTIDE COMPLEXES Deposited 1994-10-27 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Not recorded CO COBALT (II) ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions CRYSTAL SOAKED FOR NINE MONTHS AT 277 K IN STABILIZING BUFFER COMPRISING 40% (WW) MPD IN 30 MM TRIS-CL PH 8.15, 50 UM COCL2, 100 UM POTASSIUM CITRATE AND 50 UM PDTP. SOME EVAPORATION OCCURRED DURING THIS PERIOD, SO MPD CONCENTRATION AT TIME OF DATA COLLECTION, WHILE NOT KNOWN PRECISELY, WAS SUBSTANTIALLY GREATER THAN 40%.
Resolution 1.85 Å
1STN THE CRYSTAL STRUCTURE OF STAPHYLOCOCCAL NUCLEASE REFINED AT 1.7 ANGSTROMS RESOLUTION Deposited 1993-02-17 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.70 Å
1STY THE ALPHA ANEURISM: A STRUCTURAL MOTIF REVEALED IN AN INSERTION MUTANT OF STAPHYLOCOCCAL NUCLEASE Deposited 1993-02-18 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–230（148 aa）
Not recorded CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.67 Å
1SYB TRANSFER OF A BETA-TURN STRUCTURE TO A NEW PROTEIN CONTEXT Deposited 1994-01-07 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Not recorded CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å
1SYC ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE Deposited 1994-01-07 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å
1SYD ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE Deposited 1994-01-07 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Not recorded CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.70 Å
1SYE ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE Deposited 1994-01-07 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å
1SYF ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE Deposited 1994-01-07 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Not recorded CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å
1SYG ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE Deposited 1994-01-07 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å
1TQO Cryogenic Crystal Structure of Staphylococcal Nuclease Variant truncated Delta+PHS I92E Deposited 2004-06-17 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–226（144 aa）
Mutation：G50F, V51N, I92E, P117G, H124L, S128A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；300 K；MPD, potassium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 27K
Resolution 2.00 Å R-free 0.249
1TR5 Room temperature structure of Staphylococcal nuclease variant truncated Delta+PHS I92E Deposited 2004-06-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–226（144 aa）
Mutation：Deleteion of residues 44-49 and 145-149; point mutations G50F, V51N, I92E, P117G, H124L and S128A CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；MPD, potassium phosphate, glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.10 Å R-free 0.250
1TT2 Cryogenic crystal structure of Staphylococcal nuclease variant truncated Delta+PHS I92K Deposited 2004-06-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–226（144 aa）
Mutation：Deletion of residues 44-49 and 145-149; point mutations G50F, V51N, I92K, P117G, H124L, S128A CA CALCIUM ION × 1 DMS DIMETHYL SULFOXIDE × 2 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.1；277 K；MPD, potassium phosphate, glycerol, DMSO, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.85 Å R-free 0.211
1U9R Crystal Structure of Staphylococcal Nuclease mutant V66E/P117G/H124L/S128A at Room Temperature Deposited 2004-08-10 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V66E/P117G/H124L/S128A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.4；277 K；MPD, potassium phosphate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.10 Å R-free 0.217
2ENB CRYSTAL STRUCTURES OF THE BINARY CA2+ AND PDTP COMPLEXES AND THE TERNARY COMPLEX OF THE ASP 21->GLU MUTANT OF STAPHYLOCOCCAL NUCLEASE. IMPLICATIONS FOR CATALYSIS AND LIGAND BINDING Deposited 1994-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 89–223（135 aa）
Not recorded THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.05 Å
2EXZ Crystal structure of Staphylococcal nuclease mutant T22C Deposited 2005-11-09 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：T22C No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.90 Å R-free 0.265
2EY1 Crystal structure of Staphylococcal nuclease mutant T22V Deposited 2005-11-09 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：T22V No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.85 Å R-free 0.264
2EY2 Crystal structure of Staphylococcal nuclease mutant T41C Deposited 2005-11-09 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：T41C No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.70 Å R-free 0.266
2EY5 Crystal structure of Staphylococcal nuclease mutant T41S Deposited 2005-11-09 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：T41S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.270
2EY6 Crystal structure of Staphylococcal nuclease mutant T41V Deposited 2005-11-09 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：T41V No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.65 Å R-free 0.247
2EYF Crystal structure of Staphylococcal nuclease mutant T44V Deposited 2005-11-09 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：T44V No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å R-free 0.266
2EYH Crystal structure of Staphylococcal nuclease mutant T62S Deposited 2005-11-09 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：T62S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.90 Å R-free 0.261
2EYJ Crystal structure of Staphylococcal nuclease mutant T62V Deposited 2005-11-09 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：T62V No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.85 Å R-free 0.235
2EYL Crystal structure of Staphylococcal nuclease mutant T82S Deposited 2005-11-09 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：T82S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.70 Å R-free 0.249
2EYM Crystal structure of Staphylococcal nuclease mutant T120C Deposited 2005-11-09 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：T120C No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.80 Å R-free 0.263
2EYO Crystal structure of Staphylococcal nuclease mutant T120S Deposited 2005-11-09 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：T120S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.70 Å R-free 0.238
2EYP Crystal structure of Staphylococcal nuclease mutant T120V Deposited 2005-11-09 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：T120V No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.80 Å R-free 0.251
2F0D Crystal structure of Staphylococcal nuclease mutant I92V Deposited 2005-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：I92V No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.50 Å R-free 0.162
2F0E Crystal structure of Staphylococcal nuclease mutant V23L Deposited 2005-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23L No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.95 Å R-free 0.254
2F0F Crystal structure of Staphylococcal nuclease mutant L25I Deposited 2005-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：L25I No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.15 Å R-free 0.288
2F0G Crystal structure of Staphylococcal nuclease mutant V66I Deposited 2005-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V66I No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.90 Å R-free 0.252
2F0H Crystal structure of Staphylococcal nuclease mutant V66L Deposited 2005-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V66L No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.15 Å R-free 0.282
2F0I Crystal structure of Staphylococcal nuclease mutant I72L Deposited 2005-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：I72L No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.80 Å R-free 0.271
2F0J Crystal structure of Staphylococcal nuclease mutant I72V Deposited 2005-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：I72V No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.80 Å R-free 0.251
2F0K Crystal structure of Staphylococcal nuclease mutant V23I/L25I Deposited 2005-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23I/L25I No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.90 Å R-free 0.262
2F0L Crystal structure of Staphylococcal nuclease mutant V23L/I72L Deposited 2005-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23L/I72L No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.90 Å R-free 0.264
2F0M Crystal structure of Staphylococcal nuclease mutant V23L/I72V Deposited 2005-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23L/I72V No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.266
2F0N Crystal structure of Staphylococcal nuclease mutant L25I/I72L Deposited 2005-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：L25I/I72L No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.70 Å R-free 0.280
2F0O Crystal structure of Staphylococcal nuclease mutant V66I/I72V Deposited 2005-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V66I/I72V No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.90 Å R-free 0.255
2F0P Crystal structure of Staphylococcal nuclease mutant V66I/V99I Deposited 2005-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V66I/V99I No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.05 Å R-free 0.258
2F0Q Crystal structure of Staphylococcal nuclease mutant V66L/I92L Deposited 2005-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V66L/I92L No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.95 Å R-free 0.270
2F0S Crystal structure of Staphylococcal nuclease mutant V66L/I92V Deposited 2005-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V66L/I92V No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.75 Å R-free 0.270
2F0T Crystal structure of Staphylococcal nuclease mutant V66L/V99I Deposited 2005-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V66L/V99I No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.70 Å R-free 0.274
2F0U Crystal structure of Staphylococcal nuclease mutant V23I/L25I/I72V Deposited 2005-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23I/L25I/I72V No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.83 Å R-free 0.243
2F0V Crystal structure of Staphylococcal nuclease mutant V23L/V66L/I72L Deposited 2005-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23L/V66L/I72L No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.254
2F0W Crystal structure of Staphylococcal nuclease mutant V23I/L25I/V66L/I72L Deposited 2005-11-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23I/L25I/V66L/I72L No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.10 Å R-free 0.277
2F3V Solution structure of 1-110 fragment of staphylococcal nuclease with V66W mutation Deposited 2005-11-22 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–192（110 aa） Fragment：Residues 1-110
Mutation：V66W No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 4.9；298 K；Ionic strength (raw mmCIF value) 50mM ACETATE BUFFER；Pressure ambient
NMR sample composition 2mM V66W110 fragment, 50MM ACETATE BUFFER, DSS, NAN3; 90% H2O, 10% D2O | 90% H2O/10% D2O
Resolution not provided
2F3W solution structure of 1-110 fragment of staphylococcal nuclease in 2M TMAO Deposited 2005-11-22 Different construct Different oligomeric state Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–192（110 aa） Fragment：residues 1-110
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6；298 K；Ionic strength (raw mmCIF value) 2M TMAO, 0.2M HCl；Pressure ambient
NMR sample composition 1mM SNase110 fragment, 2M TMAO, 0.2M HCl, DSS, NAN3; 90% H2O, 10% D2O | 90% H2O/10% D2O
Resolution not provided
2FXY Solution structure of 55-72 segment of staphylococcal nuclease Deposited 2006-02-07 Different construct Different oligomeric state Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 137–154（18 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 5；298 K；Ionic strength (raw mmCIF value) 0；Pressure ambient
NMR sample composition 2.0mM peptides; DSS, NaN3; 40% trifluoroethanol-d4, 60% H2O | 40% trifluoroethanol-d4, 60% H2O
NMR sample composition 2.0mM peptides; DSS, NaN3; 40% trifluoroethanol-d4, 60% D2O | 40% trifluoroethanol-d4, 60% D2O
Resolution not provided
2FXZ Solution structure of 97-109 segment of staphylococcal nuclease Deposited 2006-02-07 Different construct Different oligomeric state Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 179–191（13 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 5；298 K；Ionic strength (raw mmCIF value) 0；Pressure ambient
NMR sample composition 2.0mM peptides; DSS, NaN3; 40% trifluoroethanol-d4, 60% H2O | 40% trifluoroethanol-d4, 60% H2O
NMR sample composition 2.0mM peptides; DSS, NaN3; 40% trifluoroethanol-d4, 60% D2O | 40% trifluoroethanol-d4, 60% D2O
Resolution not provided
2KHS Solution structure of SNase121:SNase(111-143) complex Deposited 2009-04-10 Different construct Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 83–203（121 aa） Fragment：UNP residues 83-203
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 5；300 K；Ionic strength (raw mmCIF value) 0.15；Pressure ambient
NMR sample composition 1.0 mM [U-13C; U-15N] protein-1, 1.0 mM protein-2, 50 mM sodium acetate-3, 100 mM potassium chloride-4, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1.0 mM protein-1, 1.0 mM [U-13C; U-15N] protein-2, 50 mM sodium acetate-7, 100 mM potassium chloride-8, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-13C] protein-1, 1 mM [U-15N] protein-2, 50 mM sodium acetate-11, 100 mM potassium chloride-12, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-15N] protein-1, 1 mM [U-13C] protein-2, 50 mM sodium acetate-15, 100 mM potassium chloride-16, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2NUC STAPHLOCOCCAL NUCLEASE, ETHANE THIOL DISULFIDE TO V23C VARIANT Deposited 1997-12-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23C Non-standard monomer：Yes (specific site not provided by mmCIF) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.15；10 MM POTASSIUM PHOSPHATE, PH 8.15 PROTEIN CONCENTRATION 2 MGS/ML, 21 PERCENT MPD, AT 4C.
Resolution 2.00 Å
2OEO Cryogenic crystal structure of Staphylococcal Nuclease variant truncated Delta+PHS I92D Deposited 2006-12-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 80–223（144 aa）
Mutation：G50F, V51N, I92D, P117G, H124L, S128A CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20-35% MPD, 10% glycerol, 5% DMSO, 10.5 mM potassium phosphate in the presence of Ca2+ and pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K
Resolution 2.00 Å R-free 0.226
2OF1 Cryogenic crystal structure of the Staphylococcal Nuclease variant truncated Delta+PHS I92W Deposited 2007-01-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 80–223（144 aa）
Mutation：G50F, V51N, I92W, P117G, H124L, S128A CA CALCIUM ION × 1 DMS DIMETHYL SULFOXIDE × 2 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；291 K；20-30% MPD, 10.5 mM potassium phosphate, 5% glycerol, 5% DMSO, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 1.92 Å R-free 0.244
2QDB Crystal structure of staphylococcal nuclease variant E75Q/D21N/T33V/T41I/S59A/P117G/S128A at 100 K Deposited 2007-06-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A
Mutation：E75Q,D21N,T33V,T41I,S59A,P117G,S128A PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；38% MPD and 25 mM potassium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.20 Å R-free 0.276
2RBM Crystal structure of Staphylococcal nuclease variant Delta+PHS I72K at cryogenic temperature Deposited 2007-09-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：G50F,V51N,I72K,P117G,S128A,Del44-49 CA CALCIUM ION × 1 PO4 PHOSPHATE ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；33% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.90 Å R-free 0.238
2RDF Crystal Structure of staphyloccocal nuclease VIAGAN/E75A variant at cryogenic temperature Deposited 2007-09-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A
Mutation：E75A,D21N,T33V,T41I,S59A,P117G,S128A PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；37% MPD, 25 mM potassium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.01 Å R-free 0.244
2RKS Crystal structure of Staphylococcal nuclease variant PHS L38K at cryogenic temperature Deposited 2007-10-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：nuclease
Mutation：L38K,P117G,H124L,S128A PO4 PHOSPHATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 10.5；277 K；45% MPD, 50 mM Potassium Phosphate, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.01 Å R-free 0.240
2SNM IN A STAPHYLOCOCCAL NUCLEASE MUTANT THE SIDE-CHAIN of A LYSINE REPLACING VALINE 66 IS FULLY BURIED IN THE HYDROPHOBIC CORE Deposited 1991-04-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V66K CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.97 Å
2SNS STAPHYLOCOCCAL NUCLEASE. PROPOSED MECHANISM OF ACTION BASED ON STRUCTURE OF ENZYME-THYMIDINE 3(PRIME),5(PRIME)-BIPHOSPHATE-CALCIUM ION COMPLEX AT 1.5-ANGSTROMS RESOLUTION Deposited 1982-05-14 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Not recorded CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.50 Å
2SOB SN-OB, OB-FOLD SUB-DOMAIN OF STAPHYLOCOCCAL NUCLEASE, NMR, 10 STRUCTURES Deposited 1995-09-15 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–185（103 aa）
Mutation：V66L, G88V, DEL(104-109) No recorded non-water small molecule SOLUTION NMR mmCIF provides none of the parsed conditions Resolution not provided
3BDC Crystal structure of Staphylococcal nuclease variant Delta+PHS at cryogenic temperature Deposited 2007-11-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：G50F/V51N/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；17% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K
Resolution 1.80 Å R-free 0.251
3C1E Crystal structure of Staphylococcal nuclease variant Delta+PHS L125K at cryogenic temperature Deposited 2008-01-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：G50F, V51N, P117G, L125K, H124L, S128A CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；32% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 9.0, vapor diffusion, hanging drop, temperature 277K
Resolution 1.90 Å R-free 0.253
3C1F Crystal structure of Staphylococcal nuclease variant Delta+PHS V104K at cryogenic temperature Deposited 2008-01-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：G50F, V51N, V104K, P117G, H124L, S128A CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；40% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.261
3D4D Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91E at cryogenic temperature Deposited 2008-05-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：G50F,V51N,Y91E,P117G,H124L,S128A,Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；25% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K
Resolution 2.10 Å R-free 0.290
3D4D Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91E at cryogenic temperature Deposited 2008-05-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 83–231（149 aa）
Mutation：G50F,V51N,Y91E,P117G,H124L,S128A,Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；25% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K
Resolution 2.10 Å R-free 0.290
3D6C Crystal structure of Staphylococcal nuclease variant PHS L38E at cryogenic temperature Deposited 2008-05-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：sequence database residues 83-231
Mutation：L38E,P117G,H124L,S128A CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；35% MPD, 25 mM Potassium Phosphate, Calcium chloride, PTP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.234
3DHQ Crystal structure of Staphylococcal nuclease variant Delta+PHS A90R at cryogenic temperature Deposited 2008-06-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：nuclease
Mutation：G50F/V51N/A90R/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；24% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTP, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.15 Å R-free 0.276
3DMU Crystal structure of Staphylococcal nuclease variant PHS T62K at cryogenic temperature Deposited 2008-07-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：T62K/P117G/H124L/S128A PO4 PHOSPHATE ION × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；44% MPD, 25 mM Potassium Phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.80 Å R-free 0.255
3E5S Crystal structure of Staphylococcal nuclease variant Delta+PHS L103K at cryogenic temperature Deposited 2008-08-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：G50F/V51N/L103K/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；35% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.00 Å R-free 0.224
3E5S Crystal structure of Staphylococcal nuclease variant Delta+PHS L103K at cryogenic temperature Deposited 2008-08-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 83–231（149 aa）
Mutation：G50F/V51N/L103K/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；35% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.00 Å R-free 0.224
3ERO Crystal structure of Staphylococcal nuclease variant Delta+PHS I72E at cryogenic temperature Deposited 2008-10-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：G50F/V51N/I72E/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, Soaked in higher % MPD before freezing, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.85 Å R-free 0.226
3ERQ Crystal structure of Staphylococcal nuclease variant L25K at cryogenic temperature Deposited 2008-10-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：L25K/G50F/V51N/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；22% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, Soaked in higher % MPD before freezing, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.10 Å R-free 0.278
3EVQ Crystal structure of Staphylococcal nuclease variant Delta+PHS L25E at cryogenic temperature Deposited 2008-10-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：L25E/G50F/V51N/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, Soaked in higher % MPD before freezing, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.258
3H6M Crystal structure of Staphylococcal nuclease variant Delta+PHS V104E at cryogenic temperature Deposited 2009-04-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：44-49 deletion, G50F, V51N, V104E, P117G, H124L, S128A THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；30% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.70 Å R-free 0.222
3HEJ Crystal structure of Staphylococcal nuclease variant Delta+PHS T62R at cryogenic temperature Deposited 2009-05-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 83–231（149 aa）
Chain D 83–231（149 aa）
Mutation：G50F, V51N, T62R, P117G, H124L, S128A, Del44-49 Mutation：G50F, V51N, T62R, P117G, H124L, S128A, Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；24% MPD, 25 mM Potassium Phosphate, Calcium Chloride, 5% Glycerol, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K
Resolution 1.80 Å R-free 0.244
3HEJ Crystal structure of Staphylococcal nuclease variant Delta+PHS T62R at cryogenic temperature Deposited 2009-05-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain B 83–231（149 aa）
Chain C 83–231（149 aa）
Mutation：G50F, V51N, T62R, P117G, H124L, S128A, Del44-49 Mutation：G50F, V51N, T62R, P117G, H124L, S128A, Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 2 GOL GLYCEROL × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；24% MPD, 25 mM Potassium Phosphate, Calcium Chloride, 5% Glycerol, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K
Resolution 1.80 Å R-free 0.244
3HZX Crystal Structure of Staphylococcal nuclease variant D+PHS/V66K at pH 9 determined at 100 K Deposited 2009-06-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：G50F/V51N/V66K/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；39 % MPD, 25 mM Potassium phosphate, pdTp, CaCl2, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.237
3ITP Crystal structure of Staphylococcal nuclease variant Delta+PHS F34K at cryogenic temperature Deposited 2009-08-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：F34K, G50F, V51N, P117G, H124L, S128A, Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；29%(w/v) MPD, 25 mM Potassium Phosphate, Calcium Chloride (3 M eq), pdTp (2 M eq), 5.5 (mg protein)/mL in stock solution; 4uL protein stock and 4uL well solution per drop; 1-2 weeks of growth, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.75 Å R-free 0.235
3LX0 Crystal structure of Staphylococcal nuclease variant Delta+PHS D21N at cryogenic temperature Deposited 2010-02-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：residues 83-231, Deletion 126-131
Mutation：D103N,G132F,V133N,P117G,H199L,S210A THP THYMIDINE-3',5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；38% MPD, 25mM Potassium Phosphate, Calcium Chloride, pdTp, pH 6.0, vapor diffusion, hanging drop, temperature 277K
Resolution 1.50 Å R-free 0.222
3MEH Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A at cryogenic temperature Deposited 2010-03-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Deletion UNP residues 126-131
Mutation：G50F,V51N,I92A,P117G,H124L,S128A CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K
Resolution 1.50 Å R-free 0.234
3MHB Crystal structure of Staphylococcal nuclease variant Delta+PHS L38A at cryogenic temperature Deposited 2010-04-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Deletion UNP residues 126-131
Mutation：L38A,G50F,V51N,P117G,H124L,S128A THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；25% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.55 Å R-free 0.238
3MVV Crystal structure of Staphylococcal nuclease variant Delta+PHS F34A at cryogenic temperature Deposited 2010-05-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Deletion UNP residues 126-131
Mutation：F34A,G132F,V133N,P199G,S210A THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；25% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 9.0, vapor diffusion, hanging drop, temperature 277K
Resolution 1.72 Å R-free 0.230
3MXP Crystal structure of Staphylococcal nuclease variant Delta+PHS T62A at cryogenic temperature Deposited 2010-05-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Deletion UNP residues 126-131
Mutation：G50F,V51N,T62A,P117G,H124L,S128A THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；25% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.61 Å R-free 0.247
3MZ5 Crystal structure of Staphylococcal nuclease variant Delta+PHS L103A at cryogenic temperature Deposited 2010-05-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Deletion UNP residues 126-131
Mutation：G50F,V51N,L103A,P117G,H124L,S128A THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.58 Å R-free 0.238
3NHH Crystal structure of Staphylococcal nuclease variant V23E-L36K at cryogenic temperature Deposited 2010-06-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23E, L36K, G50F, V51N, P117G, H124L, S128A THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.255
3NK9 Crystal structure of Staphylococcal nuclease variant Delta+PHS V74A at cryogenic temperature Deposited 2010-06-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：G50F, V51N, V74A, P117G, H124L, S128A THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；22% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K
Resolution 1.65 Å R-free 0.224
3NP8 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36A at cryogenic temperature Deposited 2010-06-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Deletion UNP residues 126-131
Mutation：L36A,G50F,V51N,P117G,H124L,S128A THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 6.0, vapor diffusion, hanging drop, temperature 277K
Resolution 1.70 Å R-free 0.230
3NQT Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A at cryogenic temperature Deposited 2010-06-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Deletion UNP residues 126-131
Mutation：G50F,V51N,V66A,P117G,H124L,S128A THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；28% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 7.0, vapor diffusion, hanging drop, temperature 277K
Resolution 1.90 Å R-free 0.246
3NUC STAPHLOCOCCAL NUCLEASE, 1-N-PROPANE THIOL DISULFIDE TO V23C VARIANT Deposited 1997-12-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23C Non-standard monomer：Yes (specific site not provided by mmCIF) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.15；10 MM POTASSIUM PHOSPHATE, PH 8.15, PROTEIN CONCENTRATION 2 MGS/ML, 21 PERCENT MPD AT 4C.
Resolution 1.90 Å
3NXW Crystal structure of Staphylococcal nuclease variant Delta+PHS L125A at cryogenic temperature Deposited 2010-07-14 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Deletion UNP residues 126-131
Not recorded THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；29% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 9.0, vapor diffusion, hanging drop, temperature 277K
Resolution 1.65 Å R-free 0.238
3OSO Crystal structure of Staphylococcal nuclease variant Delta+PHS L25A at cryogenic temperature Deposited 2010-09-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Deletion UNP residues 126-131
Mutation：L25A, G132P, V133N, P199G, H206L, S210A THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 7.0, vapor diffusion, hanging drop, temperature 277K
Resolution 1.60 Å R-free 0.206
3OWF Crystal structure of Staphylococcal nuclease variant Delta+PHS V66R at cryogenic temperature Deposited 2010-09-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 83–231（149 aa）
Mutation：G50F/V51N/V66R/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 2 CA CALCIUM ION × 2 PO4 PHOSPHATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；277 K；19% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.85 Å R-free 0.213
3P1H Crystal structure of Staphylococcal nuclease variant Delta+PHS V23K/I92A at cryogenic temperature Deposited 2010-09-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Deletion UNP residues 126-131
Mutation：V23K,G50F,V51N,I92A,P117G,H124L,S128A THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；27% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.79 Å R-free 0.231
3P75 Crystal structure of Staphylococcal nuclease variant Delta+PHS V104D at cryogenic temperature Deposited 2010-10-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 89–223（135 aa）
Mutation：G50F/V51N/V104D/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 2 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9.5；277 K；35% MPD(w/v), 25 mM CHES, Calcium Chloride, pdTp, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.90 Å R-free 0.239
3PMF Crystal structure of Staphylococcal nuclease variant Delta+PHS V23A at cryogenic temperature Deposited 2010-11-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Deletion UNP residues 126-131
Mutation：V23A, G44F, V45N, P111G, H118L, S122A THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；22% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 7.0, vapor diffusion, hanging drop, temperature 277K
Resolution 1.60 Å R-free 0.213
3QB3 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92KL25A at cryogenic temperature Deposited 2011-01-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：UNP L107A,G132F,V133N,I174K,P199G,H206L,S210A, del(126-131) THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；27% MPD, 25 mM potassium phosphate, 4.5 mg/mL (0.28 mM) protein, 0.84 mM calcium chloride, 0.56 mM pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.63 Å R-free 0.208
3QOJ Cryogenic structure of Staphylococcal nuclease variant D+PHS/V23K Deposited 2011-02-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：V23K,G50F,V51N,P117G,H124L,S128A, del(44-49) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；24% MPD, 25 mM potassium phosphate, pdTp (2 eq.), calcium chloride (3 eq.), pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.60 Å R-free 0.228
3QOL Crystal structure of Staphylococcal nuclease variant D+PHS/V23E at pH 6 determined at 100 K Deposited 2011-02-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 41–189（149 aa） Fragment：DELETION UNP RESIDUES 126-131
Mutation：V23E,G50F,V51N,P117G,H124L,S128A CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6；277 K；22% MPD, 25 mM Potassium Phosphate, pdTp, CaCl2, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.90 Å R-free 0.227
3R3O Crystal structure of Staphylococcal nuclease variant Delta+PHS T62A at cryogenic temperature and with high redundancy Deposited 2011-03-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：UNP G132F,V133N,T144A,P199G,H206L,S210A,del(126-131) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.90 Å R-free 0.223
3RUZ Crystal structure of Staphylococcal nuclease variant Delta+PHS V74K at cryogenic temperature Deposited 2011-05-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F,V51N,V74K,P117G,H124L,S128A, del(44-49) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.58 Å R-free 0.207
3S9W Crystal structure of Staphylococcal nuclease variant Delta+PHS M98G bound to Ca2+ and thymidine-5',3'-diphosphate at cryogenic temperature Deposited 2011-06-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F/V51N/M98G/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.90 Å R-free 0.211
3SHL Crystal structure of Staphylococcal nuclease variant Delta+PHS V74KL25A at cryogenic temperature Deposited 2011-06-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：L25A,G50F,V51N,V74K,P117G,H124L,S128A, del(44-49) THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.48 Å R-free 0.216
3SK4 Crystal structure of Staphylococcal nuclease variant Delta+PHS D21N/V23E at cryogenic temperature Deposited 2011-06-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：D21N/V23E/G50F/V51N/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.85 Å R-free 0.228
3SK5 Crystal structure of Staphylococcal nuclease variant Delta+PHS V39D at cryogenic temperature Deposited 2011-06-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：V39D/G50F/V51N/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；26% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.70 Å R-free 0.213
3SK6 Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23K/L36E at cryogenic temperature Deposited 2011-06-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：D21N/V23K/T33V/L36E/T41I/G50F/V51N/S59A/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.80 Å R-free 0.238
3SK8 Crystal structure of Staphylococcal nuclease variant Delta+PHS M98G apo protein at cryogenic temperature Deposited 2011-06-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F/V51N/M98G/P117G/H124L/S128A/Del44-49 PO4 PHOSPHATE ION × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；34% MPD, 25 mM potassium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.90 Å R-free 0.210
3SR1 Crystal structure of Staphylococcal nuclease variant Delta+PHS A69G bound to Ca2+ and thymidine-5',3'-diphosphate at cryogenic temperature Deposited 2011-07-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F/V51N/A69G/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.45 Å R-free 0.202
3SXH Crystal structure of Staphylococcal nuclease variant Delta+PHS I92AL103A at cryogenic temperature Deposited 2011-07-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F,V51N,I92A,L103A,P117G,H124L,S128A, del(44-49) THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.40 Å R-free 0.199
3T13 Crystal structure of Staphylococcal nuclease variant Delta+PHS A69G at cryogenic temperature Deposited 2011-07-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F/V51N/A69G/P117G/H124L/S128A/Del44-49 PO4 PHOSPHATE ION × 2 CA CALCIUM ION × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；41% MPD, 25 mM potassium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.80 Å R-free 0.199
3T13 Crystal structure of Staphylococcal nuclease variant Delta+PHS A69G at cryogenic temperature Deposited 2011-07-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F/V51N/A69G/P117G/H124L/S128A/Del44-49 PO4 PHOSPHATE ION × 1 CA CALCIUM ION × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；41% MPD, 25 mM potassium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.80 Å R-free 0.199
3T16 Crystal structure of Staphylococcal nuclease variant NVIAGA/M98G at cryogenic temperature Deposited 2011-07-21 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：D21N/T33V/T41I/S59A/M98G/P117G/S128A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；41% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.80 Å R-free 0.211
3TME Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23E at cryogenic temperature Deposited 2011-08-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：D21N/V23E/T33V/T41I/G50F/V51N/S59A/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；38% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.40 Å R-free 0.208
3TP5 Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23E/L36E at cryogenic temperature Deposited 2011-09-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：D21N/V23E/T33V/L36E/T41I/G50F/V51N/S59A/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；31% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.80 Å R-free 0.231
3TP6 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E/L103K at cryogenic temperature Deposited 2011-09-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：L36E/G50F/V51N/L103K/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.80 Å R-free 0.217
3TP7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V99E at cryogenic temperature Deposited 2011-09-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F/V51N/V99E/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.90 Å R-free 0.246
3TP8 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E at cryogenic temperature Deposited 2011-09-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：L36E/G50F/V51N/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.60 Å R-free 0.212
3U9O Crystal structure of Staphylococcal nuclease variant NVIAGA/N100G at cryogenic temperature Deposited 2011-10-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：D21N/T33V/T41I/S59A/N100G/P117G/S128A PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；40% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.95 Å R-free 0.239
3V2T Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/V66A at cryogenic temperature Deposited 2011-12-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F,V51N,V66A,I92A,P117G,H124L,S128A,del(44-49) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.80 Å R-free 0.220
3VA5 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/V23A at cryogenic temperature Deposited 2011-12-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：unp residues 83-231
Mutation：V102A, G50F,V51N,I92A,L103A,P117G,H124L,S128A, del(44-49) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.55 Å R-free 0.218
4DF7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23L/V99I at cryogenic temperature Deposited 2012-01-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：V23L,G50F,V51N,V99I,P117G,H124L,S128A,del(44-49) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.50 Å R-free 0.224
4DFA Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/L36A at cryogenic temperature Deposited 2012-01-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：L36A,G50F,V51N,I92A,P117G,H124L,S128A,del(44-49) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7.0, vapor diffusion, hanging drop, temperature 277K
Resolution 1.40 Å R-free 0.188
4DGZ Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/L125A at cryogenic temperature Deposited 2012-01-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F,V51N,I92A,P117G,H124L,L125A,S128A,del(44-49) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.47 Å R-free 0.213
4DU9 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/V74A at cryogenic temperature Deposited 2012-02-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F,V51N,I92A,P117G,H124L,S128A,del(44-49) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.63 Å R-free 0.208
4E6I Crystal structure of Staphylococcal nuclease variant NVIAGA V99G at cryogenic temperature Deposited 2012-03-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：D21N/T33V/T41I/S59A/V99G/P117G/S128A PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；35% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.268
4E6I Crystal structure of Staphylococcal nuclease variant NVIAGA V99G at cryogenic temperature Deposited 2012-03-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：D21N/T33V/T41I/S59A/V99G/P117G/S128A MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；35% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.268
4EQN Crystal structure of Staphylococcal nuclease variant Delta+PHS V23E/I72K at cryogenic temperature Deposited 2012-04-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：V23E/G50F/V51N/I72K/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.80 Å R-free 0.214
4EQO Crystal structure of Staphylococcal nuclease variant Delta+PHS V99D at cryogenic temperature Deposited 2012-04-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F/V51N/V99D/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.70 Å R-free 0.234
4EQP Crystal structure of Staphylococcal nuclease variant Delta+PHS I72D at cryogenic temperature Deposited 2012-04-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F/V51N/I72D/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.35 Å R-free 0.188
4EVO Crystal structure of Staphylococcal nuclease variant NVIAGA/E122G at cryogenic temperature Deposited 2012-04-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：D21N/T33V/T41I/S59A/P117G/E122G/S128A PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；44% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.95 Å R-free 0.227
4F7X Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/L25A at cryogenic temperature Deposited 2012-05-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：L25A,G50F,V51N,I92A,P117G,H124L,S128A,del(44-49) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.70 Å R-free 0.197
4F8M Crystal structure of Staphylococcal nuclease variant Delta+PHS V23I/I92V at cryogenic temperature Deposited 2012-05-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：V23I,G50F,V51N,I92V,P117G,H124L,S128A,del(44-49) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.60 Å R-free 0.198
4G57 Staphylococcal Nuclease double mutant I72L, I92L Deposited 2012-07-17 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 89–223（135 aa）
Mutation：I72L, I92L No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；277 K；57% MPD, 200 mM Tris.HCl buffer, approx. 6 mg/ml protein, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.81 Å R-free 0.232
4H7B Crystal Structure of Staphylococcal nuclease mutant I72V/V99L Deposited 2012-09-20 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP Residues 83-231
Mutation：I72V, V99L No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25mM Sodium Phosphate buffer, 35-60% MPD, Vapor Diffusion, Hanging Drop, Temperature 277K, pH 7.0, VAPOR DIFFUSION, HANGING DROP
Resolution 1.60 Å R-free 0.248
4HMI Crystal structure of Staphylococcal nuclease variant Delta+PHS V99K at cryogenic temperature Deposited 2012-10-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F/V51N/V99K/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, with calcium chloride and pdTp added to protein in a 3:2:1 ratio, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.75 Å R-free 0.221
4HMJ Crystal structure of Staphylococcal nuclease variant Delta+PHS L36D at cryogenic temperature Deposited 2012-10-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：L36D/G50F/V51N/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, with calcium chloride and pdTp added to protein in a 3:2:1 ratio, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.35 Å R-free 0.200
4HTH Crystal structure of Staphylococcal nuclease variant Delta+VIAGLA at cryogenic temperature Deposited 2012-11-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：T33V/T41I/G50V/V51N/S59A/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；42% MPD, 25 mM potassium phosphate, with protein:pdTp:calcium chloride in 1:2:3 molar equivalent ratio, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.75 Å R-free 0.209
4I65 Crystal Structure of Staphylococcal nuclease mutant V23I/L25V/I72V/I92V Deposited 2012-11-29 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 88–223（136 aa） Fragment：UNP Residues 83-223
Mutation：V23I, L25V, I72V, I92V No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25mM Sodium Phosphate buffer, 35-60% MPD, Vapor Diffusion, Hanging Drop, Temperature 277K, pH 7.0, VAPOR DIFFUSION, HANGING DROP
Resolution 1.61 Å R-free 0.239
4IAL Crystal structure of Staphylococcal nuclease variant Delta+PHS H121E at cryogenic temperature Deposited 2012-12-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F/V51N/P117G/H121E/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；24% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.59 Å R-free 0.239
4ID6 Crystal Structure of Staphylococcal nuclease mutant V23I/I72L Deposited 2012-12-11 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23I, I72L No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.90 Å R-free 0.241
4IUN Crystal structure of Staphylococcal nuclease variant Delta+PHS R126E at cryogenic temperature Deposited 2013-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F/V51N/P117G/H124L/R126E/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；40% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.60 Å R-free 0.179
4IZ8 Crystal structure of Staphylococcal nuclease variant Delta+PHS H8E at cryogenic temperature Deposited 2013-01-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：H8E/G50F/V51N/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；23% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.70 Å R-free 0.213
4J1M Crystal structure of Staphylococcal nuclease variant Delta+PHS R105E at cryogenic temperature Deposited 2013-02-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F/V51N/R105E/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；27% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.65 Å R-free 0.214
4J6H Crystal structure of Staphylococcal nuclease variant Delta+PHS H121Q at cryogenic temperature Deposited 2013-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F/V51N/P117G/H121Q/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.60 Å R-free 0.205
4K14 Crystal Structure of Staphylococcal nuclease mutant V66I/V99L Deposited 2013-04-04 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 88–223（136 aa） Fragment：UNP Residues 83-231
Mutation：V66I V99L No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；50mM phosphate buffer,pH 7.5 45% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.60 Å R-free 0.252
4K2K Crystal structure of Staphylococcal nuclease variant Delta+PHS L25A/L36A/I92A at cryogenic temperature Deposited 2013-04-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：L25A,L36A,G50F,V51N,I92A,P117G,H124L,S128A,del(44-49) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.65 Å R-free 0.217
4K2L Crystal structure of Staphylococcal nuclease variant Delta+PHS I92Q at cryogenic temperature Deposited 2013-04-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50P,V51N,I92Q,P117G,H124L,S128A,del(44-49) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.55 Å R-free 0.214
4K5V Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/L36A/V99T at cryogenic temperature Deposited 2013-04-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：V23T,L36A,G50F,V51N,V99T,P117G,H124L,S128A,del(44-49) THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.75 Å R-free 0.221
4K5W Crystal structure of Staphylococcal nuclease variant V23M/L25F/T62F at cryogenic temperature Deposited 2013-04-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：V23M,L25F,T62F CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；32% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.65 Å R-free 0.202
4K5X Crystal structure of Staphylococcal nuclease variant V23M/L36F at cryogenic temperature Deposited 2013-04-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：V23M,L36F THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 2 PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.65 Å R-free 0.214
4K6D Crystal structure of Staphylococcal nuclease variant V23M/T62F at cryogenic temperature Deposited 2013-04-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：V23M,T62F THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；34% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.65 Å R-free 0.213
4K8I Crystal Structure of Staphylococcal Nuclease mutant I92V/V99L Deposited 2013-04-18 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 89–223（135 aa） Fragment：UNP Residues 89-223
Mutation：I174V/V191L No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25mM Sodium Phosphate, MPD 35-60%, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.10 Å R-free 0.247
4K8J Crystal Structure of Staphylococcal nuclease mutant V23L/V66I Deposited 2013-04-18 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 89–223（135 aa）
Mutation：V105L/V148I No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25mM Sodium Phosphate, 35-60% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.251
4KD3 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25A/V66A/I92A at cryogenic temperature Deposited 2013-04-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：L25A,G50F,V51N,V66A,I92A,P117G,H124L,S128A,del(44-49) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, vapor diffusion, hanging drop, temperature 277K, VAPOR DIFFUSION, HANGING DROP
Resolution 1.75 Å R-free 0.219
4KD4 Crystal structure of Staphylococcal nuclease variant V23L/L25V/V66I/I72V at cryogenic temperature Deposited 2013-04-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：V23L,L25V,V66I,I72V CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K
Resolution 1.55 Å R-free 0.219
4KHV Crystal structure of Staphylococcal nuclease variant Delta+PHS V23S at cryogenic temperature Deposited 2013-05-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：V23S/G50F/V51N/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；26% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.60 Å R-free 0.207
4KJN Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/V66A/V99T at cryogenic temperature Deposited 2013-05-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：V23T,G50F,V51N,V66A,V99T,P117G,H124L,S128A CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.55 Å R-free 0.228
4KJO Crystal structure of Staphylococcal nuclease variant Delta+PHS L36A/V66T/V99T at cryogenic temperature Deposited 2013-05-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：L36A, G50F,V51N,V66T,V99T,P117G,H124L,S128A CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.90 Å R-free 0.231
4KTA Crystal structure of Staphylococcal nuclease variant Delta+PHS R105Q at cryogenic temperatures Deposited 2013-05-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F/V51N/R105Q/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；29% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.95 Å R-free 0.210
4KV6 Crystal structure of Staphylococcal nuclease variant Delta+PHS R126Q at cryogenic temperature Deposited 2013-05-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F/V51N/P117G/H124L/Q126R/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；24% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.55 Å R-free 0.203
4KY5 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D at pH 7 and cryogenic temperature Deposited 2013-05-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：V23D/G50F/V51N/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；28% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.40 Å R-free 0.187
4KY6 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25D at pH 6 and cryogenic temperature Deposited 2013-05-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：L25D/G50F/V51N/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.70 Å R-free 0.227
4KY7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V74D at cryogenic temperature Deposited 2013-05-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F/V51N/V74D/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.55 Å R-free 0.207
4LAA Crystal structure of Staphylococcal nuclease variant Delta+PHS L36H at cryogenic temperature Deposited 2013-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：L36H/G50F/V51N/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.58 Å R-free 0.191
4ME5 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23S/V66A at cryogenic temperature Deposited 2013-08-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：V23S, G50F,V51N,V66A,P117G,H124L,S128A CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.80 Å R-free 0.223
4MIU Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/V99T at cryogenic temperature Deposited 2013-09-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：V23T, G50F,V51N,V99T,P117G,H124L,S128A,del(44-49) THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.67 Å R-free 0.235
4N9P Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/L25A/V99T at cryogenic temperature Deposited 2013-10-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：V23T,L25A,G50F,V51N,V99T,P117G,H124L,S128A,del(44-49) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.40 Å R-free 0.196
4N9T Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A/I92S at cryogenic temperature Deposited 2013-10-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F,V51N,V66A,I92S,P117G,H124L,S128A,del(44-49) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.60 Å R-free 0.223
4NDX Crystal structure of Staphylococcal nuclease variant Delta+PHS I92N at cryogenic temperature Deposited 2013-10-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F,V51N,I92N,P117G,H124L,S128A,del(44-49) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.50 Å R-free 0.216
4NKL Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A/I92Q at cryogenic temperature Deposited 2013-11-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F,V51N,V66A,I92Q,P117G,H124L,S128A,del(44-49) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.60 Å R-free 0.219
4NMZ Crystal structure of Staphylococcal nuclease variant Delta+PHS V23Q/V66A at cryogenic temperature Deposited 2013-11-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：V23Q,G50F,V51N,V66A,P117G,H124L,S128A,del(44-49) THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.70 Å R-free 0.212
4NP5 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A/I92N at cryogenic temperature Deposited 2013-11-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F,V51N,V66A,I92N,P117G,H124L,S128A,del(44-49) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.50 Å R-free 0.214
4ODG Crystal structure of Staphylococcal nuclease variant V23I/V66I/V74I/V99I at cryogenic temperature Deposited 2014-01-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：V23I/V66I/V74I/V99I THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；24% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.73 Å R-free 0.240
4OL7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66E A109E at cryogenic temperature Deposited 2014-01-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F/V51N/V66E/A109E/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.67 Å R-free 0.191
4OL7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66E A109E at cryogenic temperature Deposited 2014-01-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F/V51N/V66E/A109E/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.67 Å R-free 0.191
4PMB Crystal structure of Staphylcoccal nuclease variant Delta+PHS I92S at cryogenic temperature Deposited 2014-05-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：Delta 44-49, G50F, V51N, I92S, P117G, H124L, S128A THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；25% MPD, 25mM potassium phosphate, calcium chloride, pdTp
Resolution 1.80 Å R-free 0.227
4PMC Crystal structure of Staphylcoccal nuclease variant Delta+PHS I72K V74K at cryogenic temperature Deposited 2014-05-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：Delta 44-49, G50F, V51N, I72K, V74K, P117G, H124L, S128A CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25% MPD, 25mM potassium phosphate, calcium chloride, pdTp
Resolution 1.65 Å R-free 0.210
4PNY Crystal structure of Staphylococcal nuclease variant Delta+PHS I72H at cryogenic temperature Deposited 2014-02-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：G50F/V51N/I72H/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.68 Å R-free 0.242
4QB4 Crystal Structure of Staphylococcal Nuclease mutant V23L/L25V/V66L Deposited 2014-05-06 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 88–223（136 aa） Fragment：UNP Residues 88-223
Mutation：V23L, L25V, V66L No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；0.6 M Ammonium sulfate, MPD 50-60%, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K, temperature 277.0K
Resolution 1.60 Å R-free 0.236
4QF4 Crystal structure of Staphylococcal nuclease variant V23M at cryogenic temperature Deposited 2014-05-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 83–231（149 aa）
Chain B 83–231（149 aa）
Mutation：V23M Mutation：V23M THP THYMIDINE-3',5'-DIPHOSPHATE × 2 CA CALCIUM ION × 2 PO4 PHOSPHATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；32% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 6.0, vapor diffusion, hanging drop, temperature 277K
Resolution 1.80 Å R-free 0.239
4QF4 Crystal structure of Staphylococcal nuclease variant V23M at cryogenic temperature Deposited 2014-05-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 83–231（149 aa）
Mutation：V23M THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；32% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 6.0, vapor diffusion, hanging drop, temperature 277K
Resolution 1.80 Å R-free 0.239
4QF4 Crystal structure of Staphylococcal nuclease variant V23M at cryogenic temperature Deposited 2014-05-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23M THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；32% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 6.0, vapor diffusion, hanging drop, temperature 277K
Resolution 1.80 Å R-free 0.239
4R8N Crystal structure of Staphylococcal nuclease variant V23I/V66I/I72V/I92V at cryogenic temperature Deposited 2014-09-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–223（141 aa） Fragment：UNP residues 83-223
Not recorded THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 PO4 PHOSPHATE ION × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.65 Å R-free 0.239
4RKB Crystal structure of Staphylococcal nuclease variant Delta+PHS V66T/V99T at cryogenic temperature Deposited 2014-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-232
Not recorded THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；30% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp , pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.88 Å R-free 0.225
4RKL Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/V66T at cryogenic temperature Deposited 2014-10-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-232
Mutation：V23T, V60T THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K
Resolution 1.66 Å R-free 0.232
4S3S Crystal structure of Staphylococcal nuclease variant Delta+PHS I92K/V23A at cryogenic temperature Deposited 2015-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：Nuclease A (UNP residues 83-231)
Mutation：V23A/G50F/I92K/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.64 Å R-free 0.216
4TRD Crystal structure of Staphylcoccal nuclease variant Delta+PHS I72K/V74K at pH 9.0 at cryogenic temperature Deposited 2014-06-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：fragment 83-231
Mutation：Delta 44-49, G50F, V51N, I72K, V74K, P117G, H124L, S128A THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25mM potassium phosphate, calcium chloride, pdTp
Resolution 1.60 Å R-free 0.195
4WOR Staphylococcal nuclease in complex with Ca2+ and thymidine-3'-5'-diphosphate (pdTp) at room temperature Deposited 2014-10-16 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Not recorded CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.15；277 K；2-methyl-2,4-pentanediol (MPD), potassium phosphate, thymidine-3',5'-diphosphate (pdTp), Ca2+
Resolution 1.60 Å R-free 0.204
4YIJ Crystal structure of Staphylcoccal nuclease variant Delta+PHS A109E at cryogenic temperature Deposited 2015-03-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：G50F/V51N/A109E/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；25% MPD, 25mM potassium phosphate, calcium chloride, pdTp
Resolution 1.64 Å R-free 0.190
4YIJ Crystal structure of Staphylcoccal nuclease variant Delta+PHS A109E at cryogenic temperature Deposited 2015-03-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 83–231（149 aa）
Mutation：G50F/V51N/A109E/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；25% MPD, 25mM potassium phosphate, calcium chloride, pdTp
Resolution 1.64 Å R-free 0.190
4ZQ3 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92T at cryogenic temperature Deposited 2015-05-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：G50F/V51N/I92T/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；35% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.90 Å R-free 0.254
4ZUI Crystal structure of Staphylococcal nuclease variant Delta+PHS V23H at cryogenic temperature Deposited 2015-05-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23H/G50F/V51N/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.70 Å R-free 0.250
4ZUJ Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91H at cryogenic temperature Deposited 2015-05-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：G50F/V51N/Y91H/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.72 Å R-free 0.207
5C3W Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H at cryogenic temperature Deposited 2015-06-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/V66H/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.72 Å R-free 0.207
5C3X Crystal structure of Staphylococcal nuclease variant Delta+PHS I72D/N100E at cryogenic temperature Deposited 2015-06-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/I72D/N100E/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.95 Å R-free 0.208
5C4H Crystal structure of Staphylococcal nuclease variant Delta+PHS I92H at cryogenic temperature Deposited 2015-06-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/I92H/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.80 Å R-free 0.196
5C4Z Crystal structure of Staphylococcal nuclease variant Delta+PHS L25H at cryogenic temperature Deposited 2015-06-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：L25H/G50F/V51N/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.62 Å R-free 0.192
5C5L Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E / L125E at cryogenic temperature Deposited 2015-06-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/T62E/P117G/H124L/L125E/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；35% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.75 Å R-free 0.191
5C5M Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D / V104E at cryogenic temperature Deposited 2015-06-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/T62D/V104E/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.70 Å R-free 0.213
5C6A Crystal structure of Staphylococcal nuclease variant Delta+PHS L25E / N100E at cryogenic temperature Deposited 2015-06-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：L25E/G50F/V51N/N100E/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.85 Å R-free 0.203
5CC4 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E/V66E at cryogenic temperature Deposited 2015-07-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/T62E/V66E/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.90 Å R-free 0.227
5CGK Crystal structure of Staphylococcal nuclease variant Delta+PHS V66D/N100E at cryogenic temperature Deposited 2015-07-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/V66D/N100E/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.85 Å R-free 0.239
5CR3 Crystal structure of Staphylococcal nuclease variant Delta+PHS V104E/L125E at cryogenic temperature Deposited 2015-07-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：K16A,G50P,V51N,V104E,P117G,H124L,L125E,S128A,del(44-49) THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.80 Å R-free 0.232
5CV4 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D/V66H at cryogenic temperature Deposited 2015-07-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23D/G50F/V51N/V66H/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.80 Å R-free 0.232
5CV5 Crystal structure of Staphylococcal nuclease variant Delta+PHS K64G/V66K/E67G at cryogenic temperature Deposited 2015-07-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：G50F/V51N/K64G/V66K/E67G/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.80 Å R-free 0.225
5CV5 Crystal structure of Staphylococcal nuclease variant Delta+PHS K64G/V66K/E67G at cryogenic temperature Deposited 2015-07-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 83–231（149 aa）
Mutation：G50F/V51N/K64G/V66K/E67G/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.80 Å R-free 0.225
5CV6 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I92E at cryogenic temperature Deposited 2015-07-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：G50F/V51N/V66H/I92E/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.95 Å R-free 0.232
5CV7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I92D at cryogenic temperature Deposited 2015-07-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：G50F/V51N/V66H/I92D/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.65 Å R-free 0.205
5CV8 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D/T62H at cryogenic temperature Deposited 2015-07-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23D/G50F/V51N/T62H/P117G/H124L/S128A/del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.58 Å R-free 0.198
5CV9 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E/V66H at cryogenic temperature Deposited 2015-07-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：L36E/G50F/V51N/V66H/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.62 Å R-free 0.220
5DAU Crystal structure of Staphylococcal nuclease variant Delta+PHS V39E/V104E at cryogenic temperature Deposited 2015-08-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 803-231
Mutation：V39E/G50F/V51N/V104E/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.50 Å R-free 0.190
5DEH Crystal structure of Staphylococcal nuclease variant Delta+PHS N100D at cryogenic temperature Deposited 2015-08-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/N100D/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.70 Å R-free 0.200
5E1F Crystal structure of Staphylococcal nuclease variant Delta+PHS G20E at cryogenic temperature Deposited 2015-09-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/I92K/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；35% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.90 Å R-free 0.228
5E3F Crystal structure of Staphylococcal nuclease variant Delta+PHS I92K at cryogenic temperature Deposited 2015-10-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：G50F/V51N/I92K/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；26% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.75 Å R-free 0.224
5EGT Crystal structure of Staphylococcal nuclease variant Delta+PHS V66E at cryogenic temperature Deposited 2015-10-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/V66E/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25mM potassium phosphate, calcium chloride, pdTp
Resolution 1.85 Å R-free 0.218
5EKK Crystal structure of Staphylococcal nuclease variant Delta+PHS V39D/L125E at cryogenic temperature Deposited 2015-11-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V39D/V51N/P117G/H124L/L125E/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 2.10 Å R-free 0.238
5EKL Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D/N100E at cryogenic temperature Deposited 2015-11-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/T62D/N100E/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 2.10 Å R-free 0.244
5F2D Crystal structure of Staphylococcal nuclease variant Delta+PHS I92N/V99T at cryogenic temperature Deposited 2015-12-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/I92N/V99T/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.70 Å R-free 0.222
5HGT Crystal structure of Staphylococcal nuclease variant Delta+PHS L25K/I92T at cryogenic temperature Deposited 2016-01-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：L25K/G50F/V51N/I92T/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.75 Å R-free 0.212
5HUR Crystal structure of Staphylococcal nuclease variant Delta+PHS L25T/I92K at cryogenic temperature Deposited 2016-01-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：L25T/G50F/V51N/I92K/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.50 Å R-free 0.231
5I6W Crystal structure of Staphylococcal nuclease variant Delta+PHS A58D at cryogenic temperature Deposited 2016-02-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 80-228
Mutation：G50F/V51N/A58D/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；18% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.90 Å R-free 0.235
5I6Y Crystal structure of Staphylococcal nuclease variant Delta+PHS A90E at cryogenic temperature Deposited 2016-02-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 80-228
Mutation：G50F/V51N/A90E/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.90 Å R-free 0.222
5I9O Crystal structure of Staphylococcal nuclease variant Delta+PHS L36K L103K at cryogenic temperature Deposited 2016-02-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：L36K/G50F/V51N/L103K/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.95 Å R-free 0.238
5I9O Crystal structure of Staphylococcal nuclease variant Delta+PHS L36K L103K at cryogenic temperature Deposited 2016-02-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：L36K/G50F/V51N/L103K/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.95 Å R-free 0.238
5I9P Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H at cryogenic temperature Deposited 2016-02-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/T62H/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；19% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.85 Å R-free 0.228
5IGB Crystal structure of Staphylococcal nuclease variant Delta+PHS L36D/V66H at cryogenic temperature Deposited 2016-02-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：L36D/G50F/V51N/V66H/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.80 Å R-free 0.226
5IGC Crystal structure of Staphylococcal nuclease variant Delta+PHS V23E/V66H at cryogenic temperature Deposited 2016-02-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：V23E/G50F/V51N/V66H/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.80 Å R-free 0.225
5IGD Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/I92E at cryogenic temperature Deposited 2016-02-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/T62H/I92E/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.80 Å R-free 0.234
5IGE Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/V99D at cryogenic temperature Deposited 2016-02-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/T62H/V99D/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；28% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.80 Å R-free 0.211
5IGF Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/V66D at cryogenic temperature Deposited 2016-02-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/T62H/V66D/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.70 Å R-free 0.221
5IGG Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I72E at cryogenic temperature Deposited 2016-02-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/V66H/I72E/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.70 Å R-free 0.237
5IIF Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D at cryogenic temperature Deposited 2016-03-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/T62D/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.85 Å R-free 0.238
5IOC Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/V99D at cryogenic temperature Deposited 2016-03-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/V66H/V99D/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；24% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.90 Å R-free 0.235
5IOD Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/V99E at cryogenic temperature Deposited 2016-03-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/V66H/V99E/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.60 Å R-free 0.231
5ISR Crystal structure of Staphylococcal nuclease variant Delta+PHS L38E at cryogenic temperature Deposited 2016-03-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：L38E/G50F/V51N/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.90 Å R-free 0.236
5J1Z Crystal structure of Staphylococcal nuclease variant Delta+PHS A90D at cryogenic temperature Deposited 2016-03-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/A90D/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.73 Å R-free 0.209
5J22 Crystal structure of Staphylococcal nuclease variant Delta+PHS L103D at cryogenic temperature Deposited 2016-03-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/L103D/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.90 Å R-free 0.235
5JAV Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91D at cryogenic temperature Deposited 2016-04-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/Y91D/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；26% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.90 Å R-free 0.245
5JOB Crystal structure of Staphylococcal nuclease variant Delta+PHS L25K/I92A at cryogenic temperature Deposited 2016-05-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：L25K/G50F/V51N/I92A/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.45 Å R-free 0.182
5K5P Crystal structure of Staphylococcal nuclease variant Delta+PHS I72K L103K at cryogenic temperature Deposited 2016-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/I72K/L103K/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.83 Å R-free 0.237
5KEE Crystal structure of Staphylococcal nuclease variant Delta+PHS L25K/I92F at cryogenic temperature Deposited 2016-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：L25K/G50F/V51N/I92F/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.50 Å R-free 0.203
5KGU Crystal structure of Staphylococcal nuclease variant Delta+PHS N118D at cryogenic temperature Deposited 2016-06-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/P117G/N118D/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.90 Å R-free 0.233
5KIX Crystal structure of Staphylococcal nuclease variant Delta+PHS I92E at cryogenic temperature Deposited 2016-06-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/I92E/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.75 Å R-free 0.223
5KRU Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E/V66K at cryogenic temperature Deposited 2016-07-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/T62E/V66K/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；28% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.60 Å R-free 0.221
5KYI Crystal structure of Staphylococcal nuclease variant Delta+PHS T62K/V66E pH 9 at cryogenic temperature Deposited 2016-07-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/T62K/V66E/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；40% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.70 Å R-free 0.203
5KYL Crystal structure of Staphylococcal nuclease variant Delta+PHS T62K/V66E pH 7 at cryogenic temperature Deposited 2016-07-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/T62K/V66E/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.85 Å R-free 0.220
5NUC STAPHYLOCOCCAL NUCLEASE, 1-N-PENTANE THIOL DISULFIDE TO V23C VARIANT Deposited 1998-01-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23C, 1-N-PENTANE THIOL DISULFIDE Non-standard monomer：Yes (specific site not provided by mmCIF) CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.15；277 K；10 MM POTASSIUM PHOSPHATE, PH 8.15 PROTEIN CONCENTRATION = 2 MGS/ML MPD =21% T=4 DEGREES C, temperature 277K
Resolution 2.10 Å
6AMF Crystal structure of Staphylococcal nuclease variant Delta+PHS V23K/L36E at cryogenic temperature Deposited 2017-08-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23K/L36E/G50F/V51N/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；24% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.85 Å R-free 0.261
6B8R Crystal structure of Staphylococcal nuclease variant Delta+PHS V23K/L36Q at cryogenic temperature Deposited 2017-10-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa）
Mutation：V23K/L36Q/G50F/V51N/P117G/H124L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.65 Å R-free 0.216
6EEG Crystal structure of Staphylcoccal nuclease variant Delta+PHS T62E at cryogenic temperature Deposited 2018-08-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/T62E/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp
Resolution 1.95 Å R-free 0.219
6EEK Crystal structure of apo Staphylcoccal nuclease variant Delta+PHS T62E/V66K, pH 7 at cryogenic temperature Deposited 2018-08-14 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/T62E/V66K/P117G/H124L/S128A/Del44-49 No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM HEPES, 29% Jeffamine ED-2001
Resolution 2.20 Å R-free 0.309
6OK8 Crystal structure of Staphylococcal nuclease variant Delta+PHS K127L at cryogenic temperature Deposited 2019-04-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/P117G/H124L/K127L/S128A/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；46% MPD, 25 mM potassium phosphate, calcium chloride, THP
Resolution 1.80 Å R-free 0.225
6U0W Crystal structure of Staphylococcal nuclease variant Delta+PHS K133M at cryogenic temperature Deposited 2019-08-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F,V51N,P117G,H124L,S128A,K133M,Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；22% MPD, 25 mM potassium phosphate, calcium chloride, THP
Resolution 1.90 Å R-free 0.273
6U0X Crystal structure of Staphylococcal nuclease variant Delta+PHS Q123D at cryogenic temperature Deposited 2019-08-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F,V51N,P117G,Q123D,H124L,S128A,Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, THP
Resolution 1.86 Å R-free 0.272
6XSB Crystal structure of Staphylococcal nuclease variant Delta+PHS A132S at cryogenic temperature Deposited 2020-07-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/P117G/H124L/S128A/A132S/Del44-49 CA CALCIUM ION × 1 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25% MPD, 25 mM potassium phosphate, calcium chloride, THP
Resolution 1.95 Å R-free 0.259
6XSC Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T at cryogenic temperature Deposited 2020-07-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：V23T/G50F/V51N/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride
Resolution 2.00 Å R-free 0.259
6XSE Crystal structure of Staphylococcal nuclease variant Delta+PHS F34Y at cryogenic temperature Deposited 2020-07-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：F34Y/G50F/V51N/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；26% MPD, 25 mM potassium phosphate, calcium chloride, THP
Resolution 2.10 Å R-free 0.279
6XSF Crystal structure of Staphylococcal nuclease variant Delta+PHS T41V at cryogenic temperature Deposited 2020-07-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；35% MPD, 25 mM potassium phosphate, calcium chloride, THP
Resolution 1.60 Å R-free 0.227
6XSG Crystal structure of Staphylococcal nuclease variant Delta+PHS V66T at cryogenic temperature Deposited 2020-07-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/V66T/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；32% MPD, 25 mM potassium phosphate, calcium chloride, THP
Resolution 2.00 Å R-free 0.247
6XSH Crystal structure of Staphylococcal nuclease variant Delta+PHS T62V at cryogenic temperature Deposited 2020-07-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/T62V/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, THP
Resolution 2.00 Å R-free 0.276
6XSI Crystal structure of Staphylococcal nuclease variant Delta+PHS V104T at cryogenic temperature Deposited 2020-07-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 83–231（149 aa） Fragment：UNP residues 83-231
Mutation：G50F/V51N/V104T/P117G/H124L/S128A/Del44-49 THP THYMIDINE-3',5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；20% MPD, 25 mM potassium phosphate, calcium chloride, THP
Resolution 2.00 Å R-free 0.271
8K2T Solution structure of full-length HtpG in complex with D131D Deposited 2023-07-13 Different construct Different oligomeric state Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 95–222（128 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7；310 K；Ionic strength (raw mmCIF value) 150；Pressure 1
NMR sample composition 500 uM [U-13C; U-15N; U-2H] Heat shock protein 90(HtpG M domain), 500 uM [U-2H, 15N; Ala- 13CH3; Met- 13CH3; Ile-d1- 13CH3; Leu, Val- 13CH3/ 13CH3; Thr- 13CH3] Heat shock protein 90(HtpG M domian), 500 uM [U-13C; U-15N; U-2H] Heat shock protein 90(HtpG N domain), 500 uM [U-100% 13C; U-100% 15N] Disordered protein(D131D), 500 uM [U-2H, 15N; Ala- 13CH3; Met- 13CH3; Ile-d1- 13CH3; Leu, Val- 13CH3/ 13CH3; Thr- 13CH3] Heat shock protein 90(HtpG N domain), 600 uM [U-2H, 15N; Ala- 13CH3; Met- 13CH3; Ile-d1- 13CH3; Leu, Val- 13CH3/ 13CH3; Thr- 13CH3] Heat shock protein 90(HtpG C domain), 500 uM [U-2H, 15N; Ala- 13CH3; Met- 13CH3; Ile-d1- 13CH3; Leu, Val- 13CH3/ 13CH3; Thr- 13CH3] Heat shock protein 90(HtpG-D131D), 500 uM [U-2H, 15N; Ala- 13CH3; Met- 13CH3; Ile-d1- 13CH3; Leu, Val- 13CH3/ 13CH3; Thr- 13CH3] Heat shock protein 90(HtpG M-D131D N), 500 uM [U-2H, 15N; Ala- 13CH3; Met- 13CH3; Ile-d1- 13CH3; Leu, Val- 13CH3/ 13CH3; Thr- 13CH3] Heat shock protein 90(HtpG M-D131D C), 93% H2O/7% D2O | 93% H2O/7% D2O
Resolution not provided
9C11 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36R at cryogenic temperature Deposited 2024-05-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 83–231（149 aa）
Chain B 83–231（149 aa）
Not recorded THP THYMIDINE-3',5'-DIPHOSPHATE × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；28% MPD 25mM K Phosphate
Resolution 1.95 Å R-free 0.261

298 other PDB entries and 310 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NUC_STAAU
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 5–75； UniProt 152–222

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 8k2r

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 8k2r
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2. Structure Basics 2. Structure Basics

Entry ID entry_id8k2r
Deposition date deposition_date2023-07-13
Structure title titleThe structure of HtpG M domain in complex with unstructured D131D binding site b
Keywords keywordsE.coli Hsp90, CHAPERONE; CHAPERONE
Experimental Method methodSOLUTION NMR
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier24.40 Å
Radius of gyration Rg (electron density) rg_electron24.60 Å
Forward intensity I(0) i08378660000.00
Molecular weight molecular_weight784670.0 kDa
Excluded volume excluded_volume983840 ų
Envelope volume envelope_volume194360 ų
Hydration-shell volume shell_volume47231 ų
Envelope diameter envelope_diameter119.4 Å
Shell Rg shell_rg39.92 Å
Envelope Rg envelope_rg37.02 Å
Shape Rg shape_rg24.59 Å
Total Rg total_rg24.86 Å
Total atoms total_atoms110260
Residues n_residues6720
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax64.0 Å
Rg (real space) rg_real22.44 Å
Rg uncertainty (real space) rg_real_error0.10 Å
I(0) (real space) i0_real7.9000e+09
I(0) uncertainty (real space) i0_real_error7.6560e+07
Rg (reciprocal space) rg_reciprocal24.74 Å
I(0) (reciprocal space) i0_reciprocal8378000000.0000
Solution quality estimate total_estimate0.6833
Solution quality rating solution_quality REASONABLE a REASONABLE solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary24.4 Å
Skewness Skewness skewness0.367
Kurtosis Kurtosis kurtosis-0.452
Angular range angular_range— – 0.3250 Å−1
Current regularization parameter α current_alpha2.6010
Highest regularization parameter α highest_alpha7901000.0000
Real-space data points n_real_points64
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.005; Oscil: 0.976; Stabil: 0.986; Sysdev: 0.000; Positv: 1.000; Valcen: 0.998; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (2)

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8. Citations (1)

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9. Files and Curves (10)