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4o6a

Mouse cyclic GMP-AMP synthase (cGAS) in complex with DNA

Method: X-RAY DIFFRACTION Dmax: 95.1 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Cyclic GMP-AMP synthase

Mus musculus

UniProt Q8C6L5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Homooligomer Protein × 2 DNA 4 PDB declaration: hexameric（6） Consistent with all polymer counts Chain A； UniProt 147–507 Chain B； UniProt 147–507 Fragment：UNP residues 147-507 DNA1 × 2 DNA2 × 2 ZN ZINC ION × 2 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；25% Methanol, 0.1M Tris-HCl, pH 8.0, 0.01M MgCl2., VAPOR DIFFUSION, HANGING DROP, temperature 293K Resolution 1.86 Å R-free 0.218

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4O6A Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4K8V Structure of cyclic GMP-AMP Synthase (cGAS) Deposited 2013-04-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 147–507（361 aa） Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
Not recorded ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.6；277 K；0.1 M HEPES, 0.1 M MgAc2, 20% PEG3350, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.208
4K8V Structure of cyclic GMP-AMP Synthase (cGAS) Deposited 2013-04-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 147–507（361 aa） Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
Not recorded ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.6；277 K；0.1 M HEPES, 0.1 M MgAc2, 20% PEG3350, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.208
4K8V Structure of cyclic GMP-AMP Synthase (cGAS) Deposited 2013-04-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 147–507（361 aa） Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
Not recorded ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.6；277 K；0.1 M HEPES, 0.1 M MgAc2, 20% PEG3350, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.208
4K8V Structure of cyclic GMP-AMP Synthase (cGAS) Deposited 2013-04-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 147–507（361 aa） Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
Not recorded ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.6；277 K；0.1 M HEPES, 0.1 M MgAc2, 20% PEG3350, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.208
4K96 Structure of Binary Complex of cGAS with Bound dsDNA Deposited 2013-04-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain A 147–507（361 aa） Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
Not recorded ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；293 K；0.1 M MES, 8% MPD, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.08 Å R-free 0.226
4K96 Structure of Binary Complex of cGAS with Bound dsDNA Deposited 2013-04-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain B 147–507（361 aa） Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
Not recorded ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；293 K；0.1 M MES, 8% MPD, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.08 Å R-free 0.226
4K97 Structure of Ternary Complex of cGAS with dsDNA and Bound ATP Deposited 2013-04-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain A 147–507（361 aa） Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.7；293 K；0.1 M HEPES, 0.2 M CaAc2, 20% PEG300, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.41 Å R-free 0.236
4K98 Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -pppG(2 ,5 )pG Deposited 2013-04-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain A 147–507（361 aa） Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5；293 K；0.1 M NaAc, 10% MPD, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.94 Å R-free 0.199
4K99 Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -pppdG(2 ,5 )pdG Deposited 2013-04-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain A 147–507（361 aa） Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 2 GH3 3'-DEOXY-GUANOSINE-5'-TRIPHOSPHATE × 1 GDO 3'-deoxy-guanosine 5'-monophosphate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.2；293 K；0.1 M NaAc, 12% MPD, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.95 Å R-free 0.212
4K9A Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -pG(2 ,5 )pA Deposited 2013-04-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain A 147–507（361 aa） Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
Not recorded ZN ZINC ION × 1 PO4 PHOSPHATE ION × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 AMP ADENOSINE MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；0.1 M MES, 40% MPD, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.26 Å R-free 0.210
4K9B Structure of Ternary Complex of cGAS with dsDNA and Bound c[G(2 ,5 )pA(3 ,5 )p] Deposited 2013-04-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain A 147–507（361 aa） Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
Not recorded ZN ZINC ION × 1 PO4 PHOSPHATE ION × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 AMP ADENOSINE MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES, 0.2 M MgCl2, 30% PEG300, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.26 Å R-free 0.217
4LEY Structure of mouse cGAS bound to 18 bp DNA Deposited 2013-06-26 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 142–507（366 aa） Fragment：Catalytic domain, UNP residues 142-507
Chain C 142–507（366 aa） Fragment：Catalytic domain, UNP residues 142-507
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；24-36% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.50 Å R-free 0.191
4LEY Structure of mouse cGAS bound to 18 bp DNA Deposited 2013-06-26 Different construct Different experimental conditions Different structure-quality metrics Assembly 2 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain B 142–507（366 aa） Fragment：Catalytic domain, UNP residues 142-507
Chain D 142–507（366 aa） Fragment：Catalytic domain, UNP residues 142-507
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；24-36% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.50 Å R-free 0.191
4LEZ Structure of mouse cGAS bound to an 18bp DNA and cGAS product Deposited 2013-06-26 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 142–507（366 aa） Fragment：mouse cGAS catalytic domain, UNP residues 142-507
Chain C 142–507（366 aa） Fragment：mouse cGAS catalytic domain, UNP residues 142-507
Not recorded ZN ZINC ION × 2 1SY cGAMP × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；24-36% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.36 Å R-free 0.247
5N6I Crystal structure of mouse cGAS in complex with 39 bp DNA Deposited 2017-02-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 6 PDB declaration: tetradecameric
Chain A 139–507（369 aa）
Chain B 139–507（369 aa）
Chain C 139–507（369 aa）
Chain D 139–507（369 aa）
Chain E 139–507（369 aa）
Chain F 139–507（369 aa）
Not recorded ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；293.15 K；0.1M Tris pH 8, 0.2M ammonium citrate pH 7, 27,5% w/v PEG3350
Resolution 3.60 Å R-free 0.256
5XZB Mouse cGAS bound to the inhibitor RU365 Deposited 2017-07-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain A 149–505（357 aa） Fragment：UNP residues 149-505
Not recorded ZN ZINC ION × 1 A9Y (3R)-3-[1-(1H-benzimidazol-2-yl)-5-hydroxy-3-methyl-1H-pyrazol-4-yl]-2-benzofuran-1(3H)-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES, pH 6.3, 26% PEG400, 0.1 M magnesium chloride
Resolution 2.13 Å R-free 0.247
5XZE Mouse cGAS bound to the inhibitor RU332 Deposited 2017-07-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain A 147–507（361 aa） Fragment：UNP residues 147-505
Not recorded ZN ZINC ION × 1 AE7 (3R)-3-[1-(3H-1lambda~4~,3-benzothiazol-2-yl)-5-hydroxy-3-methyl-1H-pyrazol-4-yl]-2-benzofuran-1(3H)-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES, pH 6.3, 25% PEG400, 0.1 M magnesium chloride
Resolution 2.18 Å R-free 0.281
5XZG Mouse cGAS bound to the inhibitor RU521 Deposited 2017-07-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain A 147–507（361 aa） Fragment：UNP residues 147-505
Not recorded ZN ZINC ION × 1 AEV 2-(4,5-dichloro-1H-benzimidazol-2-yl)-5-methyl-4-[(1R)-3-oxo-1,3-dihydro-2-benzofuran-1-yl]-1,2-dihydro-3H-pyrazol-3-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES, pH 6.9, 22.5% PEG400, 0.08 M magnesium chloride
Resolution 1.83 Å R-free 0.232
6X59 The mouse cGAS catalytic domain binding to human assembled nucleosome Deposited 2020-05-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Heteromer；Protein × 9 PDB declaration: undecameric
Chain K 142–507（366 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.98 Å
6X5A The mouse cGAS catalytic domain binding to human nucleosome that purified from HEK293T cells Deposited 2020-05-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Heteromer；Protein × 9 PDB declaration: undecameric
Chain K 142–507（366 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.36 Å
6XJD Two mouse cGAS catalytic domain binding to human assembled nucleosome Deposited 2020-06-23 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Heteromer；Protein × 10 PDB declaration: dodecameric
Chain K 142–507（366 aa）
Chain L 142–507（366 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
7A08 CryoEM Structure of cGAS Nucleosome complex Deposited 2020-08-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Heteromer；Protein × 9 PDB declaration: undecameric
Chain a 139–507（369 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.11 Å
7BUJ mcGAS bound with pppGpG Deposited 2020-04-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 61–507（447 aa）
Chain B 61–507（447 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MN MANGANESE (II) ION × 4 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.15 M Ammonium sulfate, 0.1 M Sodium HEPES, 20 % w/v PEG-4000, pH 6.8
Resolution 2.13 Å R-free 0.278
7BUM mcGAS bound with pGpA Deposited 2020-04-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–507（507 aa）
Chain B 1–507（507 aa）
Not recorded ZN ZINC ION × 2 AMP ADENOSINE MONOPHOSPHATE × 2 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Ammonium sulfate, 0.1 M MES, 30 % w/v PEG-5000 MME, pH 6.5
Resolution 3.05 Å R-free 0.280
7BUQ mcGAS bound with 23-cGAMP Deposited 2020-04-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–507（507 aa）
Chain B 1–507（507 aa）
Not recorded ZN ZINC ION × 2 1SY cGAMP × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Ammonium sulfate, 0.1 M BIS-Tris, 25 % w/v PEG-3350, pH 6.5
Resolution 3.09 Å R-free 0.296
7JO9 1:1 cGAS-nucleosome complex Deposited 2020-08-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Heteromer；Protein × 9 PDB declaration: undecameric
Chain K 142–507（366 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7JOA 2:1 cGAS-nucleosome complex Deposited 2020-08-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Heteromer；Protein × 9 PDB declaration: undecameric
Chain K 142–507（366 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7KXS Computational design of constitutively active cGAS Deposited 2020-12-04 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 147–507（361 aa）
Chain B 147–507（361 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；20 % w/v Polyethylene glycol 3,350 200 mM Potassium thiocyanate
Resolution 2.60 Å R-free 0.261
7UTT Structure of Non-hydrolyzable ATP (ApCpp) binds to Cyclic GMP AMP synthase (cGAS) through Mn coordination Deposited 2022-04-27 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa）
Chain C 147–507（361 aa）
Not recorded APC DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER × 2 ZN ZINC ION × 2 MN MANGANESE (II) ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.04 Å R-free 0.210
7UUX ATP binds to Cyclic GMP AMP synthase (cGAS) through Mg coordination Deposited 2022-04-29 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa）
Chain C 147–507（361 aa）
Mutation：E211Q, D213N Mutation：E211Q, D213N ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.26 Å R-free 0.234
7UXW Structure of ATP and GTP bind to Cyclic GMP AMP synthase (cGAS) through Mg coordination Deposited 2022-05-06 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa）
Chain C 147–507（361 aa）
Mutation：E211Q, D213N Mutation：E211Q, D213N ATP ADENOSINE-5'-TRIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.57 Å R-free 0.248
7UYQ Structure of GTP binds to Cyclic GMP AMP synthase (cGAS) through Mg coordination Deposited 2022-05-07 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa）
Chain C 147–507（361 aa）
Mutation：E211Q, D213N Mutation：E211Q, D213N GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.57 Å R-free 0.241
7UYZ Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -pppG(2 ,5 )pG Deposited 2022-05-07 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa）
Chain C 147–507（361 aa）
Not recorded MG MAGNESIUM ION × 4 ZN ZINC ION × 2 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.49 Å R-free 0.251
7UZR Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -pppG(2 ,5 )pG Deposited 2022-05-09 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Chain C 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Not recorded OKR [[(2~{R},3~{R},4~{R},5~{R})-5-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-4-[[(2~{R},3~{S},4~{R},5~{R})-5-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 2 ZN ZINC ION × 2 MN MANGANESE (II) ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.70 Å R-free 0.245
7V0C Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -pppG(2 ,5 )pG Deposited 2022-05-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa） Fragment：catalytic domain
Chain C 147–507（361 aa） Fragment：catalytic domain
Not recorded OKR [[(2~{R},3~{R},4~{R},5~{R})-5-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-4-[[(2~{R},3~{S},4~{R},5~{R})-5-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 2 ZN ZINC ION × 2 MN MANGANESE (II) ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.57 Å R-free 0.240
7V0R Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -ppcpG(2 ,5 )pA Deposited 2022-05-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa） Fragment：catalytic domain
Chain C 147–507（361 aa） Fragment：catalytic domain
Not recorded OKX [(2~{R},3~{R},4~{R},5~{R})-4-[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-5-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-3-oxidanyl-oxolan-2-yl]methoxy-[[oxidanyl(phosphonooxy)phosphoryl]methyl]phosphinic acid × 2 MG MAGNESIUM ION × 4 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.51 Å R-free 0.250
7V0W Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -pppG(2,5 )pA Deposited 2022-05-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa）
Chain C 147–507（361 aa）
Mutation：E211Q, D213N Mutation：E211Q, D213N ZN ZINC ION × 2 MN MANGANESE (II) ION × 2 AMP ADENOSINE MONOPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.66 Å R-free 0.238
8EAE Structure of Ternary Complex of cGAS with dsDNA and Bound 5-pppG(2,5)pI Deposited 2022-08-29 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa）
Chain C 147–507（361 aa）
Not recorded VLO [[(2~{R},3~{R},4~{R},5~{R})-5-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-4-[[(2~{R},3~{S},4~{R},5~{R})-3,4-bis(oxidanyl)-5-(6-oxidanylidene-1~{H}-purin-9-yl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 2 ZN ZINC ION × 2 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.56 Å R-free 0.233
8ECC Structure of Ternary Complex of cGAS with dsDNA and Bound 5-pppI(2,5)pA Deposited 2022-09-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa）
Chain C 147–507（361 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 VWX [[(2~{R},3~{R},4~{R},5~{R})-4-[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-3-oxidanyl-5-(6-oxidanylidene-1~{H}-purin-9-yl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.44 Å R-free 0.259
8G10 Structure of Ternary Complex of cGAS with dsDNA and Bound ITP and GTP Deposited 2023-02-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Chain C 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Mutation：E211Q, D213N Mutation：E211Q, D213N GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate,32%MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.47 Å R-free 0.227
8G1J Structure of Ternary Complex of cGAS with dsDNA and Bound ATP and ITP Deposited 2023-02-02 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Chain C 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Mutation：E211Q, D213N Mutation：E211Q, D213N ATP ADENOSINE-5'-TRIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.30 Å R-free 0.234
8G23 Structure of Ternary Complex of cGAS with dsDNA and Bound pppIpA Deposited 2023-02-03 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Chain C 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 VWX [[(2~{R},3~{R},4~{R},5~{R})-4-[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-3-oxidanyl-5-(6-oxidanylidene-1~{H}-purin-9-yl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.71 Å R-free 0.248
8G2P Structure of Ternary Complex of cGAS with dsDNA and Bound ATP and GTP Deposited 2023-02-06 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa）
Chain C 147–507（361 aa）
Mutation：D307N Mutation：D307N ATP ADENOSINE-5'-TRIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.52 Å R-free 0.242
8G2Q Structure of Ternary Complex of mouse cGAS with dsDNA and Bound GTP Deposited 2023-02-06 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa）
Chain C 147–507（361 aa）
Mutation：D307N Mutation：D307N GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.37 Å R-free 0.244
8GIM Structure of Ternary Complex of mouse cGAS with dsDNA and Bound ATP: with 10mM Mg2+ Deposited 2023-03-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Chain C 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MG MAGNESIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.63 Å R-free 0.230
8GIN Structure of Ternary Complex of mouse cGAS with dsDNA and Bound ATP: with 10mM Mg2+ and 0.015mM Mn2+ Deposited 2023-03-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Chain C 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MN MANGANESE (II) ION × 2 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.75 Å R-free 0.225
8GIO Structure of Ternary Complex of mouse cGAS with dsDNA and Bound ATP: with 10mM Mg2+ and 0.1mM Mn2+ Deposited 2023-03-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Chain C 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MN MANGANESE (II) ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.67 Å R-free 0.241
8GIP Structure of Ternary Complex of mouse cGAS with dsDNA and Bound ATP: with 10mM Mg2+ and 0.040mM Mn2+ Deposited 2023-03-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Chain C 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 MN MANGANESE (II) ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.70 Å R-free 0.242
8GIR Structure of Ternary Complex of mouse cGAS with dsDNA and Bound ATP: with 10mM Mg2+ and 0.2mM Mn2+ Deposited 2023-03-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Chain C 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MN MANGANESE (II) ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.50 Å R-free 0.229
8GIS Structure of Ternary Complex of mouse cGAS with dsDNA and Bound ATP: with 10mM Mg2+ and 0.5mM Mn2+ Deposited 2023-03-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Chain C 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MN MANGANESE (II) ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.46 Å R-free 0.230
8GIT Structure of Ternary Complex of mouse cGAS with dsDNA and Bound ATP: with 10mM Mg2+ and 1mM Mn2+ Deposited 2023-03-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Chain C 147–507（361 aa） Fragment：catalytic domain, residues 147-507
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MN MANGANESE (II) ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
Resolution 2.72 Å R-free 0.230
8SHK Structure of binary complex of mouse cGAS and bound ATP Deposited 2023-04-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 147–507（361 aa） Fragment：catalytic domain (UNP residues 147-507)
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M magnesium acetate tetrahydrate, 0.1 M sodium cacodylate trihydrate, pH 6.5, 20% w/v PEG8000
Resolution 1.71 Å R-free 0.228
8SHU Structure of mouse cGAS Deposited 2023-04-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 147–507（361 aa） Fragment：catalytic domain (UNP residues 147-507)
Not recorded ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M magnesium acetate tetrahydrate, 0.1 M sodium cacodylate trihydrate, pH 6.5, 20% w/v PEG8000
Resolution 1.71 Å R-free 0.211
8SHY Structure of binary complex of mouse cGAS QN and bound ATP Deposited 2023-04-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 147–507（361 aa） Fragment：catalytic domain (UNP residues 147-507)
Mutation：E211Q, D213N ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M magnesium acetate tetrahydrate, 0.1 M sodium cacodylate trihydrate, pH 6.5, 20% w/v PEG8000
Resolution 1.77 Å R-free 0.214
8SJ0 Structure of ternary complex of cGAS with dsDNA and bound 2'-dATP Deposited 2023-04-17 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa） Fragment：catalytic domain (UNP residues 147-507)
Chain C 147–507（361 aa） Fragment：catalytic domain (UNP residues 147-507)
Not recorded DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, 0.1 M Bis-Tris pH 6.5
Resolution 2.55 Å R-free 0.237
8SJ1 Structure of ternary complex of cGAS with dsDNA and bound 3'-dATP Deposited 2023-04-17 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa） Fragment：catalytic domain (UNP residues 147-507)
Chain C 147–507（361 aa） Fragment：catalytic domain (UNP residues 147-507)
Not recorded 3AT 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, 0.1 M Bis-Tris, pH 6.5
Resolution 2.81 Å R-free 0.240
8SJ2 Structure of ternary complex of cGAS with dsDNA and bound ATP and 2'-dGTP Deposited 2023-04-17 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa） Fragment：catalytic domain (UNP residues 147-507)
Chain C 147–507（361 aa） Fragment：catalytic domain (UNP residues 147-507)
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, 0.1 M Bis-Tris, pH 6.5
Resolution 2.23 Å R-free 0.234
8SKT Structure of ternary complex of mouse cGAS with dsDNA and bound ATP with 5 mM Mn2+ Deposited 2023-04-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric
Chain A 147–507（361 aa） Fragment：catalytic domain (UNP residues 147-507)
Chain C 147–507（361 aa） Fragment：catalytic domain (UNP residues 147-507)
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MN MANGANESE (II) ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, 0.1 M Bis-Tris, pH 6.5
Resolution 2.69 Å R-free 0.247
9J2Z mouse cGAS catalytic domain bound with RU.521 Deposited 2024-08-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 146–507（362 aa）
Not recorded ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.6；277 K；0.1 M HEPES, 0.1 M MgAc2, 20% PEG3350
Resolution 2.39 Å R-free 0.263
9J2Z mouse cGAS catalytic domain bound with RU.521 Deposited 2024-08-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 146–507（362 aa）
Not recorded ZN ZINC ION × 1 A1EAM (4~{S},5~{R})-2-[4,5-bis(chloranyl)-1~{H}-benzimidazol-2-yl]-5-methyl-4-[(1~{S})-3-oxidanylidene-1~{H}-2-benzofuran-1-yl]pyrazolidin-3-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.6；277 K；0.1 M HEPES, 0.1 M MgAc2, 20% PEG3350
Resolution 2.39 Å R-free 0.263
9J2Z mouse cGAS catalytic domain bound with RU.521 Deposited 2024-08-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 146–507（362 aa）
Not recorded ZN ZINC ION × 1 A1EAM (4~{S},5~{R})-2-[4,5-bis(chloranyl)-1~{H}-benzimidazol-2-yl]-5-methyl-4-[(1~{S})-3-oxidanylidene-1~{H}-2-benzofuran-1-yl]pyrazolidin-3-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.6；277 K；0.1 M HEPES, 0.1 M MgAc2, 20% PEG3350
Resolution 2.39 Å R-free 0.263
9J2Z mouse cGAS catalytic domain bound with RU.521 Deposited 2024-08-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 146–507（362 aa）
Not recorded ZN ZINC ION × 1 A1EAM (4~{S},5~{R})-2-[4,5-bis(chloranyl)-1~{H}-benzimidazol-2-yl]-5-methyl-4-[(1~{S})-3-oxidanylidene-1~{H}-2-benzofuran-1-yl]pyrazolidin-3-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.6；277 K；0.1 M HEPES, 0.1 M MgAc2, 20% PEG3350
Resolution 2.39 Å R-free 0.263
9OH4 Cryo-EM structure of cGAS tetramer in complex with BuDNA (bubble DNA) Deposited 2025-05-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 4 PDB declaration: octameric
Chain A 149–505（357 aa） Fragment：UNP residues 149-505
Chain B 149–505（357 aa） Fragment：UNP residues 149-505
Chain C 149–505（357 aa） Fragment：UNP residues 149-505
Chain D 149–505（357 aa） Fragment：UNP residues 149-505
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM Tris pH 7.5, 150mM NaCl, and 0.5mM TCEP
cryo-EM vitrification conditions Cryogen ETHANE；Filter paper blotted with blot force 2 and blot time 2s, and plunge-frozen in liquid ethane using a Vitrobot Mark IV at 4 degree Celsius and 100% humidity.
Resolution 3.48 Å

55 other PDB entries and 63 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CGAS_MOUSE
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 2–362； UniProt 147–507 Author chain B； PDBConstruct 2–362； UniProt 147–507

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 4o6a

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 4o6a
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2. Structure Basics 2. Structure Basics

Entry ID entry_id4o6a
Deposition date deposition_date2013-12-20
Structure title titleMouse cyclic GMP-AMP synthase (cGAS) in complex with DNA
Keywords keywordsimmune response, TRANSFERASE-DNA complex; TRANSFERASE/DNA
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier30.44 Å
Radius of gyration Rg (electron density) rg_electron30.10 Å
Forward intensity I(0) i0203800000.00
Molecular weight molecular_weight103860.0 kDa
Excluded volume excluded_volume125790 ų
Envelope volume envelope_volume164920 ų
Hydration-shell volume shell_volume44419 ų
Envelope diameter envelope_diameter98.1 Å
Shell Rg shell_rg38.50 Å
Envelope Rg envelope_rg29.92 Å
Shape Rg shape_rg30.10 Å
Total Rg total_rg30.76 Å
Total atoms total_atoms7230
Residues n_residues782
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax95.1 Å
Rg (real space) rg_real30.28 Å
Rg uncertainty (real space) rg_real_error0.48 Å
I(0) (real space) i0_real2.0380e+08
I(0) uncertainty (real space) i0_real_error3.1650e+06
Rg (reciprocal space) rg_reciprocal30.35 Å
I(0) (reciprocal space) i0_reciprocal203800000.0000
Solution quality estimate total_estimate0.8959
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary38.4 Å
Skewness Skewness skewness0.205
Kurtosis Kurtosis kurtosis-0.387
Angular range angular_range— – 0.2600 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha26630000.0000
Real-space data points n_real_points53
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.900; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.987; Smooth: 0.954
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (5)

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7. Fold Classification (SCOP + CATH) 4 domains

CATH v4.4 (4 domains)

Domain ID domain_id4o6aA01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology1410 — Poly(a)-polymerase, middle domain
Homologous superfamily homologous superfamily40 —
Domain ID domain_id4o6aA02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology460 — Beta Polymerase; domain 2
Homologous superfamily homologous superfamily90 —
Domain ID domain_id4o6aB01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology1410 — Poly(a)-polymerase, middle domain
Homologous superfamily homologous superfamily40 —
Domain ID domain_id4o6aB02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology460 — Beta Polymerase; domain 2
Homologous superfamily homologous superfamily90 —
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8. Citations (1)

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9. Files and Curves (10)