Cyclic GMP-AMP synthase
Mus musculus
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain C； UniProt 147–507 | Fragment：catalytic domain (UNP residues 147-507) | ZN ZINC ION × 1 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M magnesium acetate tetrahydrate, 0.1 M sodium cacodylate trihydrate, pH 6.5, 20% w/v PEG8000 | Resolution 1.71 Å R-free 0.211 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 8SHU | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 4K8V Structure of cyclic GMP-AMP Synthase (cGAS) Deposited 2013-04-18 | Different construct Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
147–507（361 aa）
Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
|
Not recorded | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；277 K；0.1 M HEPES, 0.1 M MgAc2, 20% PEG3350, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.208 |
| 4K8V Structure of cyclic GMP-AMP Synthase (cGAS) Deposited 2013-04-18 | Different construct Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
147–507（361 aa）
Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
|
Not recorded | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；277 K；0.1 M HEPES, 0.1 M MgAc2, 20% PEG3350, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.208 |
| 4K8V Structure of cyclic GMP-AMP Synthase (cGAS) Deposited 2013-04-18 | Different construct Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
147–507（361 aa）
Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
|
Not recorded | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；277 K；0.1 M HEPES, 0.1 M MgAc2, 20% PEG3350, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.208 |
| 4K8V Structure of cyclic GMP-AMP Synthase (cGAS) Deposited 2013-04-18 | Different construct Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
147–507（361 aa）
Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
|
Not recorded | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；277 K；0.1 M HEPES, 0.1 M MgAc2, 20% PEG3350, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.208 |
| 4K96 Structure of Binary Complex of cGAS with Bound dsDNA Deposited 2013-04-19 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
147–507（361 aa）
Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
|
Not recorded | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；293 K；0.1 M MES, 8% MPD, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.08 Å R-free 0.226 |
| 4K96 Structure of Binary Complex of cGAS with Bound dsDNA Deposited 2013-04-19 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain B
147–507（361 aa）
Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
|
Not recorded | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；293 K；0.1 M MES, 8% MPD, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.08 Å R-free 0.226 |
| 4K97 Structure of Ternary Complex of cGAS with dsDNA and Bound ATP Deposited 2013-04-19 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
147–507（361 aa）
Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
|
Not recorded | ZN ZINC ION × 1 MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.7；293 K；0.1 M HEPES, 0.2 M CaAc2, 20% PEG300, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.41 Å R-free 0.236 |
| 4K98 Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -pppG(2 ,5 )pG Deposited 2013-04-19 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
147–507（361 aa）
Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
|
Not recorded | ZN ZINC ION × 1 MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；293 K；0.1 M NaAc, 10% MPD, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.94 Å R-free 0.199 |
| 4K99 Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -pppdG(2 ,5 )pdG Deposited 2013-04-19 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
147–507（361 aa）
Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
|
Not recorded | ZN ZINC ION × 1 MG MAGNESIUM ION × 2 GH3 3'-DEOXY-GUANOSINE-5'-TRIPHOSPHATE × 1 GDO 3'-deoxy-guanosine 5'-monophosphate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.2；293 K；0.1 M NaAc, 12% MPD, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.95 Å R-free 0.212 |
| 4K9A Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -pG(2 ,5 )pA Deposited 2013-04-19 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
147–507（361 aa）
Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
|
Not recorded | ZN ZINC ION × 1 PO4 PHOSPHATE ION × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 AMP ADENOSINE MONOPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；0.1 M MES, 40% MPD, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.26 Å R-free 0.210 |
| 4K9B Structure of Ternary Complex of cGAS with dsDNA and Bound c[G(2 ,5 )pA(3 ,5 )p] Deposited 2013-04-19 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
147–507（361 aa）
Fragment：C-TERMINAL DOMAIN, UNP residues 147-507
|
Not recorded | ZN ZINC ION × 1 PO4 PHOSPHATE ION × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 AMP ADENOSINE MONOPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES, 0.2 M MgCl2, 30% PEG300, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.26 Å R-free 0.217 |
| 4LEY Structure of mouse cGAS bound to 18 bp DNA Deposited 2013-06-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
142–507（366 aa）
Fragment：Catalytic domain, UNP residues 142-507
Chain C
142–507（366 aa）
Fragment：Catalytic domain, UNP residues 142-507
|
Not recorded | ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；24-36% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.50 Å R-free 0.191 |
| 4LEY Structure of mouse cGAS bound to 18 bp DNA Deposited 2013-06-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain B
142–507（366 aa）
Fragment：Catalytic domain, UNP residues 142-507
Chain D
142–507（366 aa）
Fragment：Catalytic domain, UNP residues 142-507
|
Not recorded | ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；24-36% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.50 Å R-free 0.191 |
| 4LEZ Structure of mouse cGAS bound to an 18bp DNA and cGAS product Deposited 2013-06-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
142–507（366 aa）
Fragment：mouse cGAS catalytic domain, UNP residues 142-507
Chain C
142–507（366 aa）
Fragment：mouse cGAS catalytic domain, UNP residues 142-507
|
Not recorded | ZN ZINC ION × 2 1SY cGAMP × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；24-36% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.36 Å R-free 0.247 |
| 4O6A Mouse cyclic GMP-AMP synthase (cGAS) in complex with DNA Deposited 2013-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Fragment：UNP residues 147-507
Chain B
147–507（361 aa）
Fragment：UNP residues 147-507
|
Not recorded | ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；25% Methanol, 0.1M Tris-HCl, pH 8.0, 0.01M MgCl2., VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.86 Å R-free 0.218 |
| 5N6I Crystal structure of mouse cGAS in complex with 39 bp DNA Deposited 2017-02-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 6 PDB declaration: tetradecameric |
Chain A
139–507（369 aa）
Chain B
139–507（369 aa）
Chain C
139–507（369 aa）
Chain D
139–507（369 aa）
Chain E
139–507（369 aa）
Chain F
139–507（369 aa）
|
Not recorded | ZN ZINC ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293.15 K；0.1M Tris pH 8, 0.2M ammonium citrate pH 7, 27,5% w/v PEG3350
|
Resolution 3.60 Å R-free 0.256 |
| 5XZB Mouse cGAS bound to the inhibitor RU365 Deposited 2017-07-12 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
149–505（357 aa）
Fragment：UNP residues 149-505
|
Not recorded | ZN ZINC ION × 1 A9Y (3R)-3-[1-(1H-benzimidazol-2-yl)-5-hydroxy-3-methyl-1H-pyrazol-4-yl]-2-benzofuran-1(3H)-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES, pH 6.3, 26% PEG400, 0.1 M magnesium chloride
|
Resolution 2.13 Å R-free 0.247 |
| 5XZE Mouse cGAS bound to the inhibitor RU332 Deposited 2017-07-12 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
147–507（361 aa）
Fragment：UNP residues 147-505
|
Not recorded | ZN ZINC ION × 1 AE7 (3R)-3-[1-(3H-1lambda~4~,3-benzothiazol-2-yl)-5-hydroxy-3-methyl-1H-pyrazol-4-yl]-2-benzofuran-1(3H)-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES, pH 6.3, 25% PEG400, 0.1 M magnesium chloride
|
Resolution 2.18 Å R-free 0.281 |
| 5XZG Mouse cGAS bound to the inhibitor RU521 Deposited 2017-07-12 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
147–507（361 aa）
Fragment：UNP residues 147-505
|
Not recorded | ZN ZINC ION × 1 AEV 2-(4,5-dichloro-1H-benzimidazol-2-yl)-5-methyl-4-[(1R)-3-oxo-1,3-dihydro-2-benzofuran-1-yl]-1,2-dihydro-3H-pyrazol-3-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M MES, pH 6.9, 22.5% PEG400, 0.08 M magnesium chloride
|
Resolution 1.83 Å R-free 0.232 |
| 6X59 The mouse cGAS catalytic domain binding to human assembled nucleosome Deposited 2020-05-25 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Heteromer；Protein × 9 PDB declaration: undecameric |
Chain K
142–507（366 aa）
|
Not recorded | ZN ZINC ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.98 Å |
| 6X5A The mouse cGAS catalytic domain binding to human nucleosome that purified from HEK293T cells Deposited 2020-05-25 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Heteromer；Protein × 9 PDB declaration: undecameric |
Chain K
142–507（366 aa）
|
Not recorded | ZN ZINC ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.36 Å |
| 6XJD Two mouse cGAS catalytic domain binding to human assembled nucleosome Deposited 2020-06-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Heteromer；Protein × 10 PDB declaration: dodecameric |
Chain K
142–507（366 aa）
Chain L
142–507（366 aa）
|
Not recorded | ZN ZINC ION × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.80 Å |
| 7A08 CryoEM Structure of cGAS Nucleosome complex Deposited 2020-08-07 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Heteromer；Protein × 9 PDB declaration: undecameric |
Chain a
139–507（369 aa）
|
Not recorded | ZN ZINC ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.11 Å |
| 7BUJ mcGAS bound with pppGpG Deposited 2020-04-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
61–507（447 aa）
Chain B
61–507（447 aa）
|
Not recorded | ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MN MANGANESE (II) ION × 4 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.15 M Ammonium sulfate, 0.1 M Sodium HEPES, 20 % w/v PEG-4000, pH 6.8
|
Resolution 2.13 Å R-free 0.278 |
| 7BUM mcGAS bound with pGpA Deposited 2020-04-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–507（507 aa）
Chain B
1–507（507 aa）
|
Not recorded | ZN ZINC ION × 2 AMP ADENOSINE MONOPHOSPHATE × 2 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Ammonium sulfate, 0.1 M MES, 30 % w/v PEG-5000 MME, pH 6.5
|
Resolution 3.05 Å R-free 0.280 |
| 7BUQ mcGAS bound with 23-cGAMP Deposited 2020-04-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–507（507 aa）
Chain B
1–507（507 aa）
|
Not recorded | ZN ZINC ION × 2 1SY cGAMP × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Ammonium sulfate, 0.1 M BIS-Tris, 25 % w/v PEG-3350, pH 6.5
|
Resolution 3.09 Å R-free 0.296 |
| 7JO9 1:1 cGAS-nucleosome complex Deposited 2020-08-06 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Heteromer；Protein × 9 PDB declaration: undecameric |
Chain K
142–507（366 aa）
|
Not recorded | ZN ZINC ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 7JOA 2:1 cGAS-nucleosome complex Deposited 2020-08-06 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Heteromer；Protein × 9 PDB declaration: undecameric |
Chain K
142–507（366 aa）
|
Not recorded | ZN ZINC ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 7KXS Computational design of constitutively active cGAS Deposited 2020-12-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
147–507（361 aa）
Chain B
147–507（361 aa）
|
Not recorded | ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20 % w/v Polyethylene glycol 3,350
200 mM Potassium thiocyanate
|
Resolution 2.60 Å R-free 0.261 |
| 7UTT Structure of Non-hydrolyzable ATP (ApCpp) binds to Cyclic GMP AMP synthase (cGAS) through Mn coordination Deposited 2022-04-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Chain C
147–507（361 aa）
|
Not recorded | APC DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER × 2 ZN ZINC ION × 2 MN MANGANESE (II) ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.04 Å R-free 0.210 |
| 7UUX ATP binds to Cyclic GMP AMP synthase (cGAS) through Mg coordination Deposited 2022-04-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Chain C
147–507（361 aa）
|
Mutation：E211Q, D213N Mutation：E211Q, D213N | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.26 Å R-free 0.234 |
| 7UXW Structure of ATP and GTP bind to Cyclic GMP AMP synthase (cGAS) through Mg coordination Deposited 2022-05-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Chain C
147–507（361 aa）
|
Mutation：E211Q, D213N Mutation：E211Q, D213N | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.57 Å R-free 0.248 |
| 7UYQ Structure of GTP binds to Cyclic GMP AMP synthase (cGAS) through Mg coordination Deposited 2022-05-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Chain C
147–507（361 aa）
|
Mutation：E211Q, D213N Mutation：E211Q, D213N | GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.57 Å R-free 0.241 |
| 7UYZ Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -pppG(2 ,5 )pG Deposited 2022-05-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Chain C
147–507（361 aa）
|
Not recorded | MG MAGNESIUM ION × 4 ZN ZINC ION × 2 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.49 Å R-free 0.251 |
| 7UZR Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -pppG(2 ,5 )pG Deposited 2022-05-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
Chain C
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
|
Not recorded | OKR [[(2~{R},3~{R},4~{R},5~{R})-5-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-4-[[(2~{R},3~{S},4~{R},5~{R})-5-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 2 ZN ZINC ION × 2 MN MANGANESE (II) ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.70 Å R-free 0.245 |
| 7V0C Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -pppG(2 ,5 )pG Deposited 2022-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Fragment：catalytic domain
Chain C
147–507（361 aa）
Fragment：catalytic domain
|
Not recorded | OKR [[(2~{R},3~{R},4~{R},5~{R})-5-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-4-[[(2~{R},3~{S},4~{R},5~{R})-5-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 2 ZN ZINC ION × 2 MN MANGANESE (II) ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.57 Å R-free 0.240 |
| 7V0R Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -ppcpG(2 ,5 )pA Deposited 2022-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Fragment：catalytic domain
Chain C
147–507（361 aa）
Fragment：catalytic domain
|
Not recorded | OKX [(2~{R},3~{R},4~{R},5~{R})-4-[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-5-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-3-oxidanyl-oxolan-2-yl]methoxy-[[oxidanyl(phosphonooxy)phosphoryl]methyl]phosphinic acid × 2 MG MAGNESIUM ION × 4 ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.51 Å R-free 0.250 |
| 7V0W Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -pppG(2,5 )pA Deposited 2022-05-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Chain C
147–507（361 aa）
|
Mutation：E211Q, D213N Mutation：E211Q, D213N | ZN ZINC ION × 2 MN MANGANESE (II) ION × 2 AMP ADENOSINE MONOPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.66 Å R-free 0.238 |
| 8EAE Structure of Ternary Complex of cGAS with dsDNA and Bound 5-pppG(2,5)pI Deposited 2022-08-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Chain C
147–507（361 aa）
|
Not recorded | VLO [[(2~{R},3~{R},4~{R},5~{R})-5-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-4-[[(2~{R},3~{S},4~{R},5~{R})-3,4-bis(oxidanyl)-5-(6-oxidanylidene-1~{H}-purin-9-yl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 2 ZN ZINC ION × 2 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.56 Å R-free 0.233 |
| 8ECC Structure of Ternary Complex of cGAS with dsDNA and Bound 5-pppI(2,5)pA Deposited 2022-09-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Chain C
147–507（361 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 VWX [[(2~{R},3~{R},4~{R},5~{R})-4-[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-3-oxidanyl-5-(6-oxidanylidene-1~{H}-purin-9-yl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.44 Å R-free 0.259 |
| 8G10 Structure of Ternary Complex of cGAS with dsDNA and Bound ITP and GTP Deposited 2023-02-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
Chain C
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
|
Mutation：E211Q, D213N Mutation：E211Q, D213N | GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate,32%MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.47 Å R-free 0.227 |
| 8G1J Structure of Ternary Complex of cGAS with dsDNA and Bound ATP and ITP Deposited 2023-02-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
Chain C
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
|
Mutation：E211Q, D213N Mutation：E211Q, D213N | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.30 Å R-free 0.234 |
| 8G23 Structure of Ternary Complex of cGAS with dsDNA and Bound pppIpA Deposited 2023-02-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
Chain C
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 VWX [[(2~{R},3~{R},4~{R},5~{R})-4-[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-3-oxidanyl-5-(6-oxidanylidene-1~{H}-purin-9-yl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.71 Å R-free 0.248 |
| 8G2P Structure of Ternary Complex of cGAS with dsDNA and Bound ATP and GTP Deposited 2023-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Chain C
147–507（361 aa）
|
Mutation：D307N Mutation：D307N | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.52 Å R-free 0.242 |
| 8G2Q Structure of Ternary Complex of mouse cGAS with dsDNA and Bound GTP Deposited 2023-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Chain C
147–507（361 aa）
|
Mutation：D307N Mutation：D307N | GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.37 Å R-free 0.244 |
| 8GIM Structure of Ternary Complex of mouse cGAS with dsDNA and Bound ATP: with 10mM Mg2+ Deposited 2023-03-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
Chain C
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MG MAGNESIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.63 Å R-free 0.230 |
| 8GIN Structure of Ternary Complex of mouse cGAS with dsDNA and Bound ATP: with 10mM Mg2+ and 0.015mM Mn2+ Deposited 2023-03-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
Chain C
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MN MANGANESE (II) ION × 2 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.75 Å R-free 0.225 |
| 8GIO Structure of Ternary Complex of mouse cGAS with dsDNA and Bound ATP: with 10mM Mg2+ and 0.1mM Mn2+ Deposited 2023-03-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
Chain C
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MN MANGANESE (II) ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.67 Å R-free 0.241 |
| 8GIP Structure of Ternary Complex of mouse cGAS with dsDNA and Bound ATP: with 10mM Mg2+ and 0.040mM Mn2+ Deposited 2023-03-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
Chain C
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 MN MANGANESE (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.70 Å R-free 0.242 |
| 8GIR Structure of Ternary Complex of mouse cGAS with dsDNA and Bound ATP: with 10mM Mg2+ and 0.2mM Mn2+ Deposited 2023-03-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
Chain C
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MN MANGANESE (II) ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.50 Å R-free 0.229 |
| 8GIS Structure of Ternary Complex of mouse cGAS with dsDNA and Bound ATP: with 10mM Mg2+ and 0.5mM Mn2+ Deposited 2023-03-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
Chain C
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MN MANGANESE (II) ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.46 Å R-free 0.230 |
| 8GIT Structure of Ternary Complex of mouse cGAS with dsDNA and Bound ATP: with 10mM Mg2+ and 1mM Mn2+ Deposited 2023-03-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
Chain C
147–507（361 aa）
Fragment：catalytic domain, residues 147-507
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MN MANGANESE (II) ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5
|
Resolution 2.72 Å R-free 0.230 |
| 8SHK Structure of binary complex of mouse cGAS and bound ATP Deposited 2023-04-14 | Different ligand/ion Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
147–507（361 aa）
Fragment：catalytic domain (UNP residues 147-507)
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M magnesium acetate tetrahydrate, 0.1 M sodium cacodylate trihydrate, pH 6.5, 20% w/v PEG8000
|
Resolution 1.71 Å R-free 0.228 |
| 8SHY Structure of binary complex of mouse cGAS QN and bound ATP Deposited 2023-04-14 | Different mutation/modification Different ligand/ion Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
147–507（361 aa）
Fragment：catalytic domain (UNP residues 147-507)
|
Mutation：E211Q, D213N | ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M magnesium acetate tetrahydrate, 0.1 M sodium cacodylate trihydrate, pH 6.5, 20% w/v PEG8000
|
Resolution 1.77 Å R-free 0.214 |
| 8SJ0 Structure of ternary complex of cGAS with dsDNA and bound 2'-dATP Deposited 2023-04-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Fragment：catalytic domain (UNP residues 147-507)
Chain C
147–507（361 aa）
Fragment：catalytic domain (UNP residues 147-507)
|
Not recorded | DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, 0.1 M Bis-Tris pH 6.5
|
Resolution 2.55 Å R-free 0.237 |
| 8SJ1 Structure of ternary complex of cGAS with dsDNA and bound 3'-dATP Deposited 2023-04-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Fragment：catalytic domain (UNP residues 147-507)
Chain C
147–507（361 aa）
Fragment：catalytic domain (UNP residues 147-507)
|
Not recorded | 3AT 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, 0.1 M Bis-Tris, pH 6.5
|
Resolution 2.81 Å R-free 0.240 |
| 8SJ2 Structure of ternary complex of cGAS with dsDNA and bound ATP and 2'-dGTP Deposited 2023-04-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Fragment：catalytic domain (UNP residues 147-507)
Chain C
147–507（361 aa）
Fragment：catalytic domain (UNP residues 147-507)
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, 0.1 M Bis-Tris, pH 6.5
|
Resolution 2.23 Å R-free 0.234 |
| 8SKT Structure of ternary complex of mouse cGAS with dsDNA and bound ATP with 5 mM Mn2+ Deposited 2023-04-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric |
Chain A
147–507（361 aa）
Fragment：catalytic domain (UNP residues 147-507)
Chain C
147–507（361 aa）
Fragment：catalytic domain (UNP residues 147-507)
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MN MANGANESE (II) ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.15 K；0.2 M ammonium acetate, 32% MPD, 0.1 M Bis-Tris, pH 6.5
|
Resolution 2.69 Å R-free 0.247 |
| 9J2Z mouse cGAS catalytic domain bound with RU.521 Deposited 2024-08-07 | Different construct Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
146–507（362 aa）
|
Not recorded | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；277 K；0.1 M HEPES, 0.1 M MgAc2, 20% PEG3350
|
Resolution 2.39 Å R-free 0.263 |
| 9J2Z mouse cGAS catalytic domain bound with RU.521 Deposited 2024-08-07 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
146–507（362 aa）
|
Not recorded | ZN ZINC ION × 1 A1EAM (4~{S},5~{R})-2-[4,5-bis(chloranyl)-1~{H}-benzimidazol-2-yl]-5-methyl-4-[(1~{S})-3-oxidanylidene-1~{H}-2-benzofuran-1-yl]pyrazolidin-3-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；277 K；0.1 M HEPES, 0.1 M MgAc2, 20% PEG3350
|
Resolution 2.39 Å R-free 0.263 |
| 9J2Z mouse cGAS catalytic domain bound with RU.521 Deposited 2024-08-07 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
146–507（362 aa）
|
Not recorded | ZN ZINC ION × 1 A1EAM (4~{S},5~{R})-2-[4,5-bis(chloranyl)-1~{H}-benzimidazol-2-yl]-5-methyl-4-[(1~{S})-3-oxidanylidene-1~{H}-2-benzofuran-1-yl]pyrazolidin-3-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；277 K；0.1 M HEPES, 0.1 M MgAc2, 20% PEG3350
|
Resolution 2.39 Å R-free 0.263 |
| 9J2Z mouse cGAS catalytic domain bound with RU.521 Deposited 2024-08-07 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
146–507（362 aa）
|
Not recorded | ZN ZINC ION × 1 A1EAM (4~{S},5~{R})-2-[4,5-bis(chloranyl)-1~{H}-benzimidazol-2-yl]-5-methyl-4-[(1~{S})-3-oxidanylidene-1~{H}-2-benzofuran-1-yl]pyrazolidin-3-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；277 K；0.1 M HEPES, 0.1 M MgAc2, 20% PEG3350
|
Resolution 2.39 Å R-free 0.263 |
| 9OH4 Cryo-EM structure of cGAS tetramer in complex with BuDNA (bubble DNA) Deposited 2025-05-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 4 PDB declaration: octameric |
Chain A
149–505（357 aa）
Fragment：UNP residues 149-505
Chain B
149–505（357 aa）
Fragment：UNP residues 149-505
Chain C
149–505（357 aa）
Fragment：UNP residues 149-505
Chain D
149–505（357 aa）
Fragment：UNP residues 149-505
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；20mM Tris pH 7.5, 150mM NaCl, and 0.5mM TCEP
cryo-EM vitrification conditions
Cryogen ETHANE；Filter paper blotted with blot force 2 and blot time 2s, and plunge-frozen in liquid ethane using a Vitrobot Mark IV at 4 degree Celsius and 100% humidity.
|
Resolution 3.48 Å |
55 other PDB entries and 63 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | CGAS_MOUSE |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain C； PDBConstruct 4–364； UniProt 147–507 |
