4r18

Ligand-induced Lys33-Thr1 crosslinking at subunit beta5 of the yeast 20S proteasome

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

PROTEASOME SUBUNIT ALPHA TYPE-2

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	PROTEASOME SUBUNIT ALPHA TYPE-3 × 2 （P23638） PROTEASOME SUBUNIT ALPHA TYPE-4 × 2 （P40303） PROTEASOME SUBUNIT ALPHA TYPE-5 × 2 （P32379） PROTEASOME SUBUNIT ALPHA TYPE-6 × 2 （P40302） PROTEASOME SUBUNIT ALPHA TYPE-7 × 2 （P21242） PROTEASOME SUBUNIT ALPHA TYPE-1 × 2 （P21243） PROTEASOME SUBUNIT BETA TYPE-2 × 2 （P25043） PROTEASOME SUBUNIT BETA TYPE-3 × 2 （P25451） PROTEASOME SUBUNIT BETA TYPE-4 × 2 （P22141） PROTEASOME SUBUNIT BETA TYPE-5 × 2 （P30656） PROTEASOME SUBUNIT BETA TYPE-6 × 2 （P23724） PROTEASOME SUBUNIT BETA TYPE-7 × 2 （P30657） PROTEASOME SUBUNIT BETA TYPE-1 × 2 （P38624） MAGNESIUM ION × 11 ALPHA-AMINOBUTYRIC ACID × 2 water × 28	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSA2_YEAST
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–250； UniProt 1–250 Author chain O； PDBConstruct 1–250； UniProt 1–250

PROTEASOME SUBUNIT ALPHA TYPE-3

OrganismNot specified

UniProt P23638

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	PROTEASOME SUBUNIT ALPHA TYPE-2 × 2 （P23639） PROTEASOME SUBUNIT ALPHA TYPE-4 × 2 （P40303） PROTEASOME SUBUNIT ALPHA TYPE-5 × 2 （P32379） PROTEASOME SUBUNIT ALPHA TYPE-6 × 2 （P40302） PROTEASOME SUBUNIT ALPHA TYPE-7 × 2 （P21242） PROTEASOME SUBUNIT ALPHA TYPE-1 × 2 （P21243） PROTEASOME SUBUNIT BETA TYPE-2 × 2 （P25043） PROTEASOME SUBUNIT BETA TYPE-3 × 2 （P25451） PROTEASOME SUBUNIT BETA TYPE-4 × 2 （P22141） PROTEASOME SUBUNIT BETA TYPE-5 × 2 （P30656） PROTEASOME SUBUNIT BETA TYPE-6 × 2 （P23724） PROTEASOME SUBUNIT BETA TYPE-7 × 2 （P30657） PROTEASOME SUBUNIT BETA TYPE-1 × 2 （P38624） MAGNESIUM ION × 11 ALPHA-AMINOBUTYRIC ACID × 2 water × 28	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSA3_YEAST
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–258； UniProt 1–258 Author chain P； PDBConstruct 1–258； UniProt 1–258

PROTEASOME SUBUNIT ALPHA TYPE-4

OrganismNot specified

UniProt P40303

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	PROTEASOME SUBUNIT ALPHA TYPE-2 × 2 （P23639） PROTEASOME SUBUNIT ALPHA TYPE-3 × 2 （P23638） PROTEASOME SUBUNIT ALPHA TYPE-5 × 2 （P32379） PROTEASOME SUBUNIT ALPHA TYPE-6 × 2 （P40302） PROTEASOME SUBUNIT ALPHA TYPE-7 × 2 （P21242） PROTEASOME SUBUNIT ALPHA TYPE-1 × 2 （P21243） PROTEASOME SUBUNIT BETA TYPE-2 × 2 （P25043） PROTEASOME SUBUNIT BETA TYPE-3 × 2 （P25451） PROTEASOME SUBUNIT BETA TYPE-4 × 2 （P22141） PROTEASOME SUBUNIT BETA TYPE-5 × 2 （P30656） PROTEASOME SUBUNIT BETA TYPE-6 × 2 （P23724） PROTEASOME SUBUNIT BETA TYPE-7 × 2 （P30657） PROTEASOME SUBUNIT BETA TYPE-1 × 2 （P38624） MAGNESIUM ION × 11 ALPHA-AMINOBUTYRIC ACID × 2 water × 28	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSA4_YEAST
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain C； PDBConstruct 1–254； UniProt 1–254 Author chain Q； PDBConstruct 1–254； UniProt 1–254

PROTEASOME SUBUNIT ALPHA TYPE-5

OrganismNot specified

UniProt P32379

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	PROTEASOME SUBUNIT ALPHA TYPE-2 × 2 （P23639） PROTEASOME SUBUNIT ALPHA TYPE-3 × 2 （P23638） PROTEASOME SUBUNIT ALPHA TYPE-4 × 2 （P40303） PROTEASOME SUBUNIT ALPHA TYPE-6 × 2 （P40302） PROTEASOME SUBUNIT ALPHA TYPE-7 × 2 （P21242） PROTEASOME SUBUNIT ALPHA TYPE-1 × 2 （P21243） PROTEASOME SUBUNIT BETA TYPE-2 × 2 （P25043） PROTEASOME SUBUNIT BETA TYPE-3 × 2 （P25451） PROTEASOME SUBUNIT BETA TYPE-4 × 2 （P22141） PROTEASOME SUBUNIT BETA TYPE-5 × 2 （P30656） PROTEASOME SUBUNIT BETA TYPE-6 × 2 （P23724） PROTEASOME SUBUNIT BETA TYPE-7 × 2 （P30657） PROTEASOME SUBUNIT BETA TYPE-1 × 2 （P38624） MAGNESIUM ION × 11 ALPHA-AMINOBUTYRIC ACID × 2 water × 28	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSA5_YEAST
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain D； PDBConstruct 1–260； UniProt 1–260 Author chain R； PDBConstruct 1–260； UniProt 1–260

PROTEASOME SUBUNIT ALPHA TYPE-6

OrganismNot specified

UniProt P40302

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	PROTEASOME SUBUNIT ALPHA TYPE-2 × 2 （P23639） PROTEASOME SUBUNIT ALPHA TYPE-3 × 2 （P23638） PROTEASOME SUBUNIT ALPHA TYPE-4 × 2 （P40303） PROTEASOME SUBUNIT ALPHA TYPE-5 × 2 （P32379） PROTEASOME SUBUNIT ALPHA TYPE-7 × 2 （P21242） PROTEASOME SUBUNIT ALPHA TYPE-1 × 2 （P21243） PROTEASOME SUBUNIT BETA TYPE-2 × 2 （P25043） PROTEASOME SUBUNIT BETA TYPE-3 × 2 （P25451） PROTEASOME SUBUNIT BETA TYPE-4 × 2 （P22141） PROTEASOME SUBUNIT BETA TYPE-5 × 2 （P30656） PROTEASOME SUBUNIT BETA TYPE-6 × 2 （P23724） PROTEASOME SUBUNIT BETA TYPE-7 × 2 （P30657） PROTEASOME SUBUNIT BETA TYPE-1 × 2 （P38624） MAGNESIUM ION × 11 ALPHA-AMINOBUTYRIC ACID × 2 water × 28	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSA6_YEAST
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain E； PDBConstruct 1–234； UniProt 1–234 Author chain S； PDBConstruct 1–234； UniProt 1–234

PROTEASOME SUBUNIT ALPHA TYPE-7

OrganismNot specified

UniProt P21242

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	PROTEASOME SUBUNIT ALPHA TYPE-2 × 2 （P23639） PROTEASOME SUBUNIT ALPHA TYPE-3 × 2 （P23638） PROTEASOME SUBUNIT ALPHA TYPE-4 × 2 （P40303） PROTEASOME SUBUNIT ALPHA TYPE-5 × 2 （P32379） PROTEASOME SUBUNIT ALPHA TYPE-6 × 2 （P40302） PROTEASOME SUBUNIT ALPHA TYPE-1 × 2 （P21243） PROTEASOME SUBUNIT BETA TYPE-2 × 2 （P25043） PROTEASOME SUBUNIT BETA TYPE-3 × 2 （P25451） PROTEASOME SUBUNIT BETA TYPE-4 × 2 （P22141） PROTEASOME SUBUNIT BETA TYPE-5 × 2 （P30656） PROTEASOME SUBUNIT BETA TYPE-6 × 2 （P23724） PROTEASOME SUBUNIT BETA TYPE-7 × 2 （P30657） PROTEASOME SUBUNIT BETA TYPE-1 × 2 （P38624） MAGNESIUM ION × 11 ALPHA-AMINOBUTYRIC ACID × 2 water × 28	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSA7_YEAST
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain F； PDBConstruct 1–288； UniProt 1–288 Author chain T； PDBConstruct 1–288； UniProt 1–288

PROTEASOME SUBUNIT ALPHA TYPE-1

OrganismNot specified

UniProt P21243

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	PROTEASOME SUBUNIT ALPHA TYPE-2 × 2 （P23639） PROTEASOME SUBUNIT ALPHA TYPE-3 × 2 （P23638） PROTEASOME SUBUNIT ALPHA TYPE-4 × 2 （P40303） PROTEASOME SUBUNIT ALPHA TYPE-5 × 2 （P32379） PROTEASOME SUBUNIT ALPHA TYPE-6 × 2 （P40302） PROTEASOME SUBUNIT ALPHA TYPE-7 × 2 （P21242） PROTEASOME SUBUNIT BETA TYPE-2 × 2 （P25043） PROTEASOME SUBUNIT BETA TYPE-3 × 2 （P25451） PROTEASOME SUBUNIT BETA TYPE-4 × 2 （P22141） PROTEASOME SUBUNIT BETA TYPE-5 × 2 （P30656） PROTEASOME SUBUNIT BETA TYPE-6 × 2 （P23724） PROTEASOME SUBUNIT BETA TYPE-7 × 2 （P30657） PROTEASOME SUBUNIT BETA TYPE-1 × 2 （P38624） MAGNESIUM ION × 11 ALPHA-AMINOBUTYRIC ACID × 2 water × 28	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSA1_YEAST
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain G； PDBConstruct 1–252； UniProt 1–252 Author chain U； PDBConstruct 1–252； UniProt 1–252

PROTEASOME SUBUNIT BETA TYPE-2

OrganismNot specified

UniProt P25043

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	PROTEASOME SUBUNIT ALPHA TYPE-2 × 2 （P23639） PROTEASOME SUBUNIT ALPHA TYPE-3 × 2 （P23638） PROTEASOME SUBUNIT ALPHA TYPE-4 × 2 （P40303） PROTEASOME SUBUNIT ALPHA TYPE-5 × 2 （P32379） PROTEASOME SUBUNIT ALPHA TYPE-6 × 2 （P40302） PROTEASOME SUBUNIT ALPHA TYPE-7 × 2 （P21242） PROTEASOME SUBUNIT ALPHA TYPE-1 × 2 （P21243） PROTEASOME SUBUNIT BETA TYPE-3 × 2 （P25451） PROTEASOME SUBUNIT BETA TYPE-4 × 2 （P22141） PROTEASOME SUBUNIT BETA TYPE-5 × 2 （P30656） PROTEASOME SUBUNIT BETA TYPE-6 × 2 （P23724） PROTEASOME SUBUNIT BETA TYPE-7 × 2 （P30657） PROTEASOME SUBUNIT BETA TYPE-1 × 2 （P38624） MAGNESIUM ION × 11 ALPHA-AMINOBUTYRIC ACID × 2 water × 28	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSB2_YEAST
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain H； PDBConstruct 1–232； UniProt 30–261 Author chain V； PDBConstruct 1–232； UniProt 30–261

PROTEASOME SUBUNIT BETA TYPE-3

OrganismNot specified

UniProt P25451

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	PROTEASOME SUBUNIT ALPHA TYPE-2 × 2 （P23639） PROTEASOME SUBUNIT ALPHA TYPE-3 × 2 （P23638） PROTEASOME SUBUNIT ALPHA TYPE-4 × 2 （P40303） PROTEASOME SUBUNIT ALPHA TYPE-5 × 2 （P32379） PROTEASOME SUBUNIT ALPHA TYPE-6 × 2 （P40302） PROTEASOME SUBUNIT ALPHA TYPE-7 × 2 （P21242） PROTEASOME SUBUNIT ALPHA TYPE-1 × 2 （P21243） PROTEASOME SUBUNIT BETA TYPE-2 × 2 （P25043） PROTEASOME SUBUNIT BETA TYPE-4 × 2 （P22141） PROTEASOME SUBUNIT BETA TYPE-5 × 2 （P30656） PROTEASOME SUBUNIT BETA TYPE-6 × 2 （P23724） PROTEASOME SUBUNIT BETA TYPE-7 × 2 （P30657） PROTEASOME SUBUNIT BETA TYPE-1 × 2 （P38624） MAGNESIUM ION × 11 ALPHA-AMINOBUTYRIC ACID × 2 water × 28	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSB3_YEAST
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain I； PDBConstruct 1–205； UniProt 1–205 Author chain W； PDBConstruct 1–205； UniProt 1–205

PROTEASOME SUBUNIT BETA TYPE-4

OrganismNot specified

UniProt P22141

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	PROTEASOME SUBUNIT ALPHA TYPE-2 × 2 （P23639） PROTEASOME SUBUNIT ALPHA TYPE-3 × 2 （P23638） PROTEASOME SUBUNIT ALPHA TYPE-4 × 2 （P40303） PROTEASOME SUBUNIT ALPHA TYPE-5 × 2 （P32379） PROTEASOME SUBUNIT ALPHA TYPE-6 × 2 （P40302） PROTEASOME SUBUNIT ALPHA TYPE-7 × 2 （P21242） PROTEASOME SUBUNIT ALPHA TYPE-1 × 2 （P21243） PROTEASOME SUBUNIT BETA TYPE-2 × 2 （P25043） PROTEASOME SUBUNIT BETA TYPE-3 × 2 （P25451） PROTEASOME SUBUNIT BETA TYPE-5 × 2 （P30656） PROTEASOME SUBUNIT BETA TYPE-6 × 2 （P23724） PROTEASOME SUBUNIT BETA TYPE-7 × 2 （P30657） PROTEASOME SUBUNIT BETA TYPE-1 × 2 （P38624） MAGNESIUM ION × 11 ALPHA-AMINOBUTYRIC ACID × 2 water × 28	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSB4_YEAST
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain J； PDBConstruct 1–198； UniProt 1–198 Author chain X； PDBConstruct 1–198； UniProt 1–198

PROTEASOME SUBUNIT BETA TYPE-5

OrganismNot specified

UniProt P30656

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	PROTEASOME SUBUNIT ALPHA TYPE-2 × 2 （P23639） PROTEASOME SUBUNIT ALPHA TYPE-3 × 2 （P23638） PROTEASOME SUBUNIT ALPHA TYPE-4 × 2 （P40303） PROTEASOME SUBUNIT ALPHA TYPE-5 × 2 （P32379） PROTEASOME SUBUNIT ALPHA TYPE-6 × 2 （P40302） PROTEASOME SUBUNIT ALPHA TYPE-7 × 2 （P21242） PROTEASOME SUBUNIT ALPHA TYPE-1 × 2 （P21243） PROTEASOME SUBUNIT BETA TYPE-2 × 2 （P25043） PROTEASOME SUBUNIT BETA TYPE-3 × 2 （P25451） PROTEASOME SUBUNIT BETA TYPE-4 × 2 （P22141） PROTEASOME SUBUNIT BETA TYPE-6 × 2 （P23724） PROTEASOME SUBUNIT BETA TYPE-7 × 2 （P30657） PROTEASOME SUBUNIT BETA TYPE-1 × 2 （P38624） MAGNESIUM ION × 11 ALPHA-AMINOBUTYRIC ACID × 2 water × 28	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSB5_YEAST
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain K； PDBConstruct 1–212； UniProt 76–287 Author chain Y； PDBConstruct 1–212； UniProt 76–287

PROTEASOME SUBUNIT BETA TYPE-6

OrganismNot specified

UniProt P23724

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	PROTEASOME SUBUNIT ALPHA TYPE-2 × 2 （P23639） PROTEASOME SUBUNIT ALPHA TYPE-3 × 2 （P23638） PROTEASOME SUBUNIT ALPHA TYPE-4 × 2 （P40303） PROTEASOME SUBUNIT ALPHA TYPE-5 × 2 （P32379） PROTEASOME SUBUNIT ALPHA TYPE-6 × 2 （P40302） PROTEASOME SUBUNIT ALPHA TYPE-7 × 2 （P21242） PROTEASOME SUBUNIT ALPHA TYPE-1 × 2 （P21243） PROTEASOME SUBUNIT BETA TYPE-2 × 2 （P25043） PROTEASOME SUBUNIT BETA TYPE-3 × 2 （P25451） PROTEASOME SUBUNIT BETA TYPE-4 × 2 （P22141） PROTEASOME SUBUNIT BETA TYPE-5 × 2 （P30656） PROTEASOME SUBUNIT BETA TYPE-7 × 2 （P30657） PROTEASOME SUBUNIT BETA TYPE-1 × 2 （P38624） MAGNESIUM ION × 11 ALPHA-AMINOBUTYRIC ACID × 2 water × 28	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSB6_YEAST
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain L； PDBConstruct 1–222； UniProt 20–241 Author chain Z； PDBConstruct 1–222； UniProt 20–241

PROTEASOME SUBUNIT BETA TYPE-7

OrganismNot specified

UniProt P30657

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	PROTEASOME SUBUNIT ALPHA TYPE-2 × 2 （P23639） PROTEASOME SUBUNIT ALPHA TYPE-3 × 2 （P23638） PROTEASOME SUBUNIT ALPHA TYPE-4 × 2 （P40303） PROTEASOME SUBUNIT ALPHA TYPE-5 × 2 （P32379） PROTEASOME SUBUNIT ALPHA TYPE-6 × 2 （P40302） PROTEASOME SUBUNIT ALPHA TYPE-7 × 2 （P21242） PROTEASOME SUBUNIT ALPHA TYPE-1 × 2 （P21243） PROTEASOME SUBUNIT BETA TYPE-2 × 2 （P25043） PROTEASOME SUBUNIT BETA TYPE-3 × 2 （P25451） PROTEASOME SUBUNIT BETA TYPE-4 × 2 （P22141） PROTEASOME SUBUNIT BETA TYPE-5 × 2 （P30656） PROTEASOME SUBUNIT BETA TYPE-6 × 2 （P23724） PROTEASOME SUBUNIT BETA TYPE-1 × 2 （P38624） MAGNESIUM ION × 11 ALPHA-AMINOBUTYRIC ACID × 2 water × 28	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSB7_YEAST
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain M； PDBConstruct 1–246； UniProt 21–266 Author chain a； PDBConstruct 1–246； UniProt 21–266

PROTEASOME SUBUNIT BETA TYPE-1

OrganismNot specified

UniProt P38624

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	PROTEASOME SUBUNIT ALPHA TYPE-2 × 2 （P23639） PROTEASOME SUBUNIT ALPHA TYPE-3 × 2 （P23638） PROTEASOME SUBUNIT ALPHA TYPE-4 × 2 （P40303） PROTEASOME SUBUNIT ALPHA TYPE-5 × 2 （P32379） PROTEASOME SUBUNIT ALPHA TYPE-6 × 2 （P40302） PROTEASOME SUBUNIT ALPHA TYPE-7 × 2 （P21242） PROTEASOME SUBUNIT ALPHA TYPE-1 × 2 （P21243） PROTEASOME SUBUNIT BETA TYPE-2 × 2 （P25043） PROTEASOME SUBUNIT BETA TYPE-3 × 2 （P25451） PROTEASOME SUBUNIT BETA TYPE-4 × 2 （P22141） PROTEASOME SUBUNIT BETA TYPE-5 × 2 （P30656） PROTEASOME SUBUNIT BETA TYPE-6 × 2 （P23724） PROTEASOME SUBUNIT BETA TYPE-7 × 2 （P30657） MAGNESIUM ION × 11 ALPHA-AMINOBUTYRIC ACID × 2 water × 28	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSB1_YEAST
Isoform	—
PDB entities	14
Chains and sequence ranges	Author chain N； PDBConstruct 1–196； UniProt 20–215 Author chain b； PDBConstruct 1–196； UniProt 20–215

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	4r18
Deposition date deposition_date	2014-08-04
Structure title title	Ligand-induced Lys33-Thr1 crosslinking at subunit beta5 of the yeast 20S proteasome
Keywords keywords	Proteasome, Drug Development, Binding Analysis, Umpolung, Crosslink, HYDROLASE-HYDROLASE INHIBITOR complex; HYDROLASE/HYDROLASE INHIBITOR
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

4r18__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

4r18__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

4r18__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	60.49 Å
Rg (electron density)	60.03 Å
Total Rg	60.24 Å
Atom count	49375
Residues	6342
Excluded volume	879060 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`4r18__assembly_1__model_1`	28-meric （28）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (17)

Entity 1 Entity 1 polymer

Description description	PROTEASOME SUBUNIT ALPHA TYPE-2
Molecular weight formula_weight	27191.8 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,O
Atom count atom_count	51115

MTDRYSFSLTTFSPSGKLGQIDYALTAVKQGVTSLGIKATNGVVIATEKKSSSPLAMSETLSKVSLLTPDIGAVYSGMGPDYRVLVDKSRKVAHTSYKRIYGEYPPTKLLVSEVAKIMQEATQSGGVRPFGVSLLIAGHDEFNGFSLYQVDPSGSYFPWKATAIGKGSVAAKTFLEKRWNDELELEDAIHIALLTLKESVEGEFNGDTIELAIIGDENPDLLGYTGIPTDKGPRFRKLTSQEINDRLEAL

Entity 2 Entity 2 polymer

Description description	PROTEASOME SUBUNIT ALPHA TYPE-3
Molecular weight formula_weight	28748.2 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B,P

MGSRRYDSRTTIFSPEGRLYQVEYALESISHAGTAIGIMASDGIVLAAERKVTSTLLEQDTSTEKLYKLNDKIAVAVAGLTADAEILINTARIHAQNYLKTYNEDIPVEILVRRLSDIKQGYTQHGGLRPFGVSFIYAGYDDRYGYQLYTSNPSGNYTGWKAISVGANTSAAQTLLQMDYKDDMKVDDAIELALKTLSKTTDSSALTYDRLEFATIRKGANDGEVYQKIFKPQEIKDILVKTGITKKDEDEEADEDMK

Entity 3 Entity 3 polymer

Description description	PROTEASOME SUBUNIT ALPHA TYPE-4
Molecular weight formula_weight	28478.1 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C,Q

MSGYDRALSIFSPDGHIFQVEYALEAVKRGTCAVGVKGKNCVVLGCERRSTLKLQDTRITPSKVSKIDSHVVLSFSGLNADSRILIEKARVEAQSHRLTLEDPVTVEYLTRYVAGVQQRYTQSGGVRPFGVSTLIAGFDPRDDEPKLYQTEPSGIYSSWSAQTIGRNSKTVREFLEKNYDRKEPPATVEECVKLTVRSLLEVVQTGAKNIEITVVKPDSDIVALSSEEINQYVTQIEQEKQEQQEQDKKKKSNH

Entity 4 Entity 4 polymer

Description description	PROTEASOME SUBUNIT ALPHA TYPE-5
Molecular weight formula_weight	28649.1 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D,R

MFLTRSEYDRGVSTFSPEGRLFQVEYSLEAIKLGSTAIGIATKEGVVLGVEKRATSPLLESDSIEKIVEIDRHIGCAMSGLTADARSMIEHARTAAVTHNLYYDEDINVESLTQSVCDLALRFGEGASGEERLMSRPFGVALLIAGHDADDGYQLFHAEPSGTFYRYNAKAIGSGSEGAQAELLNEWHSSLTLKEAELLVLKILKQVMEEKLDENNAQLSCITKQDGFKIYDNEKTAELIKELKEKEAAESPEEADVEMS

Entity 5 Entity 5 polymer

Description description	PROTEASOME SUBUNIT ALPHA TYPE-6
Molecular weight formula_weight	25634.0 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E,S

MFRNNYDGDTVTFSPTGRLFQVEYALEAIKQGSVTVGLRSNTHAVLVALKRNADELSSYQKKIIKCDEHMGLSLAGLAPDARVLSNYLRQQCNYSSLVFNRKLAVERAGHLLCDKAQKNTQSYGGRPYGVGLLIIGYDKSGAHLLEFQPSGNVTELYGTAIGARSQGAKTYLERTLDTFIKIDGNPDELIKAGVEAISQSLRDESLTVDNLSIAIVGKDTPFTIYDGEAVAKYI

Entity 6 Entity 6 polymer

Description description	PROTEASOME SUBUNIT ALPHA TYPE-7
Molecular weight formula_weight	31575.1 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F,T

MTSIGTGYDLSNSVFSPDGRNFQVEYAVKAVENGTTSIGIKCNDGVVFAVEKLITSKLLVPQKNVKIQVVDRHIGCVYSGLIPDGRHLVNRGREEAASFKKLYKTPIPIPAFADRLGQYVQAHTLYNSVRPFGVSTIFGGVDKNGAHLYMLEPSGSYWGYKGAATGKGRQSAKAELEKLVDHHPEGLSAREAVKQAAKIIYLAHEDNKEKDFELEISWCSLSETNGLHKFVKGDLLQEAIDFAQKEINGDDDEDEDDSDNVMSSDDENAPVATNANATTDQEGDIHLE

Entity 7 Entity 7 polymer

Description description	PROTEASOME SUBUNIT ALPHA TYPE-1
Molecular weight formula_weight	28033.8 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G,U

MSGAAAASAAGYDRHITIFSPEGRLYQVEYAFKATNQTNINSLAVRGKDCTVVISQKKVPDKLLDPTTVSYIFCISRTIGMVVNGPIPDARNAALRAKAEAAEFRYKYGYDMPCDVLAKRMANLSQIYTQRAYMRPLGVILTFVSVDEELGPSIYKTDPAGYYVGYKATATGPKQQEITTNLENHFKKSKIDHINEESWEKVVEFAITHMIDALGTEFSKNDLEVGVATKDKFFTLSAENIEERLVAIAEQD

Entity 8 Entity 8 polymer

Description description	PROTEASOME SUBUNIT BETA TYPE-2
Molecular weight formula_weight	25114.5 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H,V

TTIVGVKFNNGVVIAADTRSTQGPIVADKNCAKLHRISPKIWCAGAGTAADTEAVTQLIGSNIELHSLYTSREPRVVSALQMLKQHLFKYQGHIGAYLIVAGVDPTGSHLFSIHAHGSTDVGYYLSLGSGSLAAMAVLESHWKQDLTKEEAIKLASDAIQAGIWNDLGSGSNVDVCVMEIGKDAEYLRNYLTPNVREEKQKSYKFPRGTTAVLKESIVNICDIQEEQVDITA

Entity 9 Entity 9 polymer

Description description	PROTEASOME SUBUNIT BETA TYPE-3
Molecular weight formula_weight	22627.8 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I,W

MSDPSSINGGIVVAMTGKDCVAIACDLRLGSQSLGVSNKFEKIFHYGHVFLGITGLATDVTTLNEMFRYKTNLYKLKEERAIEPETFTQLVSSSLYERRFGPYFVGPVVAGINSKSGKPFIAGFDLIGCIDEAKDFIVSGTASDQLFGMCESLYEPNLEPEDLFETISQALLNAADRDALSGWGAVVYIIKKDEVVKRYLKMRQD

Entity 10 Entity 10 polymer

Description description	PROTEASOME SUBUNIT BETA TYPE-4
Molecular weight formula_weight	22545.7 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J,X

MDIILGIRVQDSVILASSKAVTRGISVLKDSDDKTRQLSPHTLMSFAGEAGDTVQFAEYIQANIQLYSIREDYELSPQAVSSFVRQELAKSIRSRRPYQVNVLIGGYDKKKNKPELYQIDYLGTKVELPYGAHGYSGFYTFSLLDHHYRPDMTTEEGLDLLKLCVQELEKRMPMDFKGVIVKIVDKDGIRQVDDFQAQ

Entity 11 Entity 11 polymer

Description description	PROTEASOME SUBUNIT BETA TYPE-5
Molecular weight formula_weight	23325.2 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	K,Y

TTTLAFRFQGGIIVAVDSRATAGNWVASQTVKKVIEINPFLLGTMAGGAADCQFWETWLGSQCRLHELREKERISVAAASKILSNLVYQYKGAGLSMGTMICGYTRKEGPTIYYVDSDGTRLKGDIFCVGSGQTFAYGVLDSNYKWDLSVEDALYLGKRSILAAAHRDAYSGGSVNLYHVTEDGWIYHGNHDVGELFWKVKEEEGSFNNVIG

Entity 12 Entity 12 polymer

Description description	PROTEASOME SUBUNIT BETA TYPE-6
Molecular weight formula_weight	24883.9 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	L,Z

QFNPYGDNGGTILGIAGEDFAVLAGDTRNITDYSINSRYEPKVFDCGDNIVMSANGFAADGDALVKRFKNSVKWYHFDHNDKKLSINSAARNIQHLLYGKRFFPYYVHTIIAGLDEDGKGAVYSFDPVGSYEREQCRAGGAAASLIMPFLDNQVNFKNQYEPGTNGKVKKPLKYLSVEEVIKLVRDSFTSATERHIQVGDGLEILIVTKDGVRKEFYELKRD

Entity 13 Entity 13 polymer

Description description	PROTEASOME SUBUNIT BETA TYPE-7
Molecular weight formula_weight	27200.9 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	M,a

TQIANAGASPMVNTQQPIVTGTSVISMKYDNGVIIAADNLGSYGSLLRFNGVERLIPVGDNTVVGISGDISDMQHIERLLKDLVTENAYDNPLADAEEALEPSYIFEYLATVMYQRRSKMNPLWNAIIVAGVQSNGDQFLRYVNLLGVTYSSPTLATGFGAHMANPLLRKVVDRESDIPKTTVQVAEEAIVNAMRVLYYRDARSSRNFSLAIIDKNTGLTFKKNLQVENMKWDFAKDIKGYGTQKI

Entity 14 Entity 14 polymer

Description description	PROTEASOME SUBUNIT BETA TYPE-1
Molecular weight formula_weight	21517.2 kDa
EC 编号 ec_number	3.4.25.1
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	N,b

TSIMAVTFKDGVILGADSRTTTGAYIANRVTDKLTRVHDKIWCCRSGSAADTQAIADIVQYHLELYTSQYGTPSTETAASVFKELCYENKDNLTAGIIVAGYDDKNKGEVYTIPLGGSVHKLPYAIAGSGSTFIYGYCDKNFRENMSKEETVDFIKHSLSQAIKWDGSSGGVIRMVVLTAAGVERLIFYPDEYEQL

Entity 15 Entity 15 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	11

Entity 16 Entity 16 non-polymer

Description description	ALPHA-AMINOBUTYRIC ACID
Molecular weight formula_weight	103.1 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 17 Entity 17 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	1740

▼

6. Fold Classification (SCOP + CATH) 56 domains

SCOP 2.08 (28 domains)

Domain ID domain_id	d4r18a_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18b_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18c1
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18c2
Class class	l — Artifacts
Fold Fold fold	l.1 — Tags
Superfamily Superfamily superfamily	l.1.1 — Tags
Family Family family	l.1.1.1 — Tags

Domain ID domain_id	d4r18d_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18e_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18f_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18g_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18h_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18i_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18j_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18k_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18l_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18m_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18n_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18o_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18p_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18q1
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18q2
Class class	l — Artifacts
Fold Fold fold	l.1 — Tags
Superfamily Superfamily superfamily	l.1.1 — Tags
Family Family family	l.1.1.1 — Tags

Domain ID domain_id	d4r18r_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18s_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18t_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18u_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18v_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18w_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18x_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18y_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

Domain ID domain_id	d4r18z_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.153 — Ntn hydrolase-like
Superfamily Superfamily superfamily	d.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family family	d.153.1.4 — Proteasome subunits

CATH v4.4 (28 domains)

Domain ID domain_id	4r18A00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18B00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18C00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18D00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18E00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18F00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18G00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18H00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18I00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18J00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18K00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18L00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18M00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18N00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18O00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18P00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18Q00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18R00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18S00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18T00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18U00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18V00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18W00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18X00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18Y00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18Z00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18a00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

Domain ID domain_id	4r18b00
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily	10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain

▶

Unit-cell length a length_a	136.580 Å
Unit-cell length b length_b	300.820 Å
Unit-cell length c length_c	146.290 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	113.20 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	4

Space group space_group	P 1 21 1
Space-group number int_tables_number	4
Crystal system crystal_system	—

Resolution resolution	2.400 Å
Low-resolution limit resolution_low	15.000 Å
R factor r_factor	0.1781
R_work r_work	0.1772
R_free r_free	0.1949

Ligand-induced Lys33-Thr1 crosslinking at subunit beta5 of the yeast 20S proteasome

1. Protein Identity and Related Structures Protein Identity & Related Structures

PROTEASOME SUBUNIT ALPHA TYPE-2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

PROTEASOME SUBUNIT ALPHA TYPE-3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

PROTEASOME SUBUNIT ALPHA TYPE-4

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

PROTEASOME SUBUNIT ALPHA TYPE-5

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

PROTEASOME SUBUNIT ALPHA TYPE-6

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

PROTEASOME SUBUNIT ALPHA TYPE-7

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

PROTEASOME SUBUNIT ALPHA TYPE-1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

PROTEASOME SUBUNIT BETA TYPE-2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

PROTEASOME SUBUNIT BETA TYPE-3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

PROTEASOME SUBUNIT BETA TYPE-4

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer